ORF
Human Ortholog
Description Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified gene MRPL38
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0.12 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.1
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.87 0.79 0.76 0.79 0.87 0.83 – 0.78 0.67 0.63 0.64 0.63 0.79 0.91 0.96 0.56 0.57 0.52 0.79 0.79 0.76 0.77 0.77 0.54
Endoplasmic Reticulum 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0.06 0 0.08 0 0 0 0 0 0.07
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0.07 0 0 0 0 0 0.05
Mitochondria 0 0 0.18 0.17 0.07 0.33 – 0.41 0.6 0.55 0.52 0.58 0 0 0 0 0.06 0 0 0 0.05 0 0 0
Nucleus 0 0 0.06 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0.07 0.06 0 0.06 0 0
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0.13 0 0.09 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0.07
Vac/Vac Membrane 0.12 0.17 0 0.11 0.09 0 – 0 0 0 0 0 0.23 0.08 0 0.31 0.32 0.3 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 – 0 0 0 1 1 0 0 0 0 0 1 0 0 0 0 2 7
Bud 0 0 2 4 3 4 – 7 7 8 4 6 2 1 2 0 0 0 5 3 1 4 21 42
Bud Neck 0 0 0 0 0 0 – 2 0 3 0 0 0 0 0 0 0 0 2 0 0 0 1 4
Bud Site 0 0 0 0 0 0 – 1 0 0 0 1 0 0 0 0 0 0 – – – – – –
Cell Periphery 1 0 0 0 0 1 – 2 1 2 4 2 0 0 1 1 0 0 0 0 0 0 0 0
Cytoplasm 227 46 13 120 215 192 – 406 183 200 112 162 261 429 519 53 39 47 210 76 46 79 282 241
Endoplasmic Reticulum 2 0 0 0 2 0 – 0 0 1 0 3 1 1 1 1 1 4 0 0 0 0 1 3
Endosome 2 1 0 1 3 1 – 2 0 2 1 1 8 5 4 6 3 7 6 1 1 0 8 29
Golgi 1 0 0 1 0 0 – 0 0 1 0 0 1 0 0 3 2 6 3 0 0 0 2 22
Mitochondria 7 2 3 26 17 77 – 213 163 176 90 148 4 12 6 4 4 4 8 1 2 0 3 11
Nucleus 3 0 1 0 1 0 – 6 0 8 3 3 3 0 1 1 0 0 18 5 1 6 13 17
Nuclear Periphery 0 0 0 1 0 0 – 3 1 1 0 2 0 0 0 0 0 0 1 0 0 0 0 1
Nucleolus 1 1 0 0 0 0 – 2 0 2 1 0 2 5 1 12 3 8 0 0 0 1 1 1
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 3 1 1 1 0 0 1 0 0 0 5
SpindlePole 0 0 0 0 0 0 – 5 2 1 1 2 1 1 1 1 0 1 3 1 1 6 10 30
Vac/Vac Membrane 32 10 0 17 23 2 – 16 3 5 7 3 75 37 15 29 22 27 5 2 2 4 13 19
Unique Cell Count 260 58 17 152 248 231 521 272 319 174 256 332 474 538 95 68 90 268 96 61 103 369 444
Labelled Cell Count 276 60 19 170 264 277 665 360 410 224 334 358 494 552 112 75 105 268 96 61 103 369 444


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 7.2 6.8 4.9 6.1 5.7 3.5 – 3.5 3.0 3.5 3.2 3.6 10.6 9.1 6.6 18.9 16.6 17.7 7.2 7.2 7.5
Std Deviation (1e-4) 1.7 2.0 1.5 3.3 1.8 1.2 – 1.4 1.3 1.8 1.6 8.6 3.6 3.9 1.8 13.9 7.5 9.2 3.4 2.2 2.3
Intensity Change (Log2) – – – 0.32 0.22 -0.47 – -0.48 -0.72 -0.48 -0.59 -0.44 1.11 0.9 0.44 1.96 1.77 1.86 0.56 0.55 0.62


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.2 1.2 0.7 – 0.1 -0.8 -1.1 -1.0 -1.1 0.2 1.9 4.1 -1.6 -1.4 -1.8
Endoplasmic Reticulum 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 3.9248 7.4421 6.3978 5.7154 5.1401 6.6306 5.196 9.7457 7.5961 6.5702 7.8193 7.5345 7.3681 10.7155 9.8428 8.428 8.9045 9.0336
Actin 0.0308 0.0026 0.0264 0.0573 0.0059 0.0072 0.037 0.0004 0.0088 0.0176 0.0069 0.0021 0.0622 0.0005 0.003 0.0084 0.0163 0.0024
Bud 0.001 0.0009 0.0016 0.0011 0.0008 0.0004 0.0016 0.0003 0.0006 0.0005 0.0018 0.0003 0.0006 0.0002 0.0003 0.0022 0.0004 0.0003
Bud Neck 0.0054 0.0005 0.0018 0.0097 0.0012 0.0017 0.0075 0.0004 0.0016 0.0252 0.0018 0.0023 0.0033 0.0003 0.0008 0.0127 0.001 0.0017
Bud Periphery 0.0006 0.0004 0.0009 0.0029 0.001 0.0005 0.0014 0.0002 0.0006 0.0003 0.0005 0.0002 0.0004 0 0.0001 0.0032 0.0006 0.0002
Bud Site 0.0065 0.0014 0.0022 0.0025 0.0038 0.0004 0.0075 0.0005 0.0018 0.0009 0.0008 0.0005 0.0008 0.0004 0.0037 0.003 0.0006 0.0002
Cell Periphery 0.0004 0.0001 0.0001 0.0017 0.0001 0 0.001 0 0.0001 0.0001 0.0001 0 0.0001 0 0.0001 0.0003 0.0002 0
Cytoplasm 0.6952 0.844 0.6719 0.6221 0.6868 0.6947 0.6143 0.7499 0.6688 0.6007 0.6543 0.6269 0.6828 0.8004 0.7385 0.6927 0.7231 0.7022
Cytoplasmic Foci 0.0378 0.0036 0.0169 0.0166 0.0178 0.019 0.0381 0.0123 0.0271 0.0163 0.0539 0.0148 0.0077 0.0022 0.0054 0.0022 0.0038 0.0032
Eisosomes 0.0003 0.0001 0.0001 0.0005 0.0001 0.0001 0.0007 0 0 0.0001 0 0 0.0003 0 0.0002 0 0.0004 0
Endoplasmic Reticulum 0.0143 0.0029 0.0057 0.0043 0.0039 0.0054 0.0122 0.0026 0.0046 0.0042 0.0058 0.0035 0.0116 0.0024 0.0027 0.0023 0.0142 0.003
Endosome 0.0539 0.0034 0.0273 0.0177 0.0349 0.0172 0.0539 0.0034 0.0227 0.0332 0.0295 0.0149 0.0187 0.0011 0.0025 0.004 0.0109 0.0044
Golgi 0.0083 0.0006 0.0154 0.0212 0.011 0.0047 0.01 0.0004 0.0069 0.0221 0.002 0.0033 0.0042 0.0001 0.0003 0.0018 0.0024 0.0048
Lipid Particles 0.0107 0.0002 0.0086 0.0087 0.0071 0.0051 0.0094 0.0002 0.007 0.0108 0.0034 0.0024 0.003 0 0.001 0.0003 0.0012 0.0044
Mitochondria 0.0023 0.0089 0.0074 0.0195 0.0551 0.0128 0.0074 0.0063 0.0092 0.0111 0.0017 0.0074 0.0021 0.0002 0.0013 0.0011 0.0083 0.0067
None 0.0329 0.0038 0.0082 0.0088 0.0093 0.0049 0.0184 0.0114 0.003 0.0026 0.0068 0.002 0.0339 0.0023 0.0111 0.0013 0.0034 0.0017
Nuclear Periphery 0.0202 0.008 0.0142 0.0104 0.0129 0.0174 0.0321 0.0129 0.0154 0.0153 0.0196 0.0175 0.0247 0.0095 0.0101 0.0117 0.0229 0.0153
Nucleolus 0.0007 0.0001 0.0006 0.0004 0.0005 0.0005 0.0008 0.0001 0.0004 0.0007 0.0005 0.0002 0.0004 0.0001 0.0004 0.0003 0.0003 0.0004
Nucleus 0.0574 0.113 0.1732 0.1717 0.0856 0.1945 0.1199 0.185 0.1995 0.2179 0.1867 0.2907 0.1308 0.1779 0.2029 0.2461 0.1813 0.2406
Peroxisomes 0.0045 0.0002 0.006 0.0083 0.0508 0.0024 0.007 0.0004 0.0073 0.0128 0.0051 0.0012 0.002 0.0001 0.0014 0.0004 0.0003 0.0008
Punctate Nuclear 0.0094 0.0035 0.0079 0.0045 0.0069 0.0062 0.0106 0.0118 0.0083 0.004 0.0154 0.007 0.0065 0.0014 0.0131 0.003 0.0047 0.0062
Vacuole 0.006 0.0014 0.0026 0.007 0.0029 0.0034 0.0067 0.0011 0.0048 0.0028 0.0026 0.0021 0.0024 0.0008 0.0007 0.0024 0.0021 0.001
Vacuole Periphery 0.0013 0.0005 0.001 0.003 0.0014 0.0015 0.0024 0.0003 0.0013 0.0007 0.0008 0.0005 0.0013 0.0002 0.0002 0.0005 0.0017 0.0005

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression – – – – – – – – – –
Translational Efficiency – – – – – – – – – –

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression –
Translational Efficiency –
ORF
Human Ortholog
Description Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified gene MRPL38
Localization
Cell Percentages cytoplasm (54%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

YKL169C

YKL169C


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ykl169c-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available