Standard name
Human Ortholog
Description Mitochondrial ribosomal protein of the large subunit; appears as two protein spots (YmL34 and YmL38) on two-dimensional SDS gels; protein abundance increases in response to DNA replication stress
DOWNLOAD

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0.15 0.09 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0.1 0 0.05 0 0.05 0
Bud 0.06 0.06 0.19 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0.22 0.49 0.38 0.45 0.26 0.19 – 0.12 0.15 0.21 0.2 0.14 0.49 0.63 0.5 0.6 0.34 0.71 0.12 0.23 0.38 0.23 0.2 0.19
Cytoplasm 0.7 0.59 0.51 0.47 0.53 0.67 – 0.66 0.75 0.69 0.75 0.68 0.57 0.59 0.57 0.59 0.85 0.67 0.29 0.46 0.19 0.32 0.29 0.34
Endoplasmic Reticulum 0.25 0.18 0.11 0.31 0.39 0.29 – 0.3 0.19 0.19 0.15 0.24 0.19 0.15 0.17 0 0.06 0 0.39 0.22 0.3 0.28 0.3 0.28
Endosome 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 – 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0.09 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 4 5 25 17 6 1 – 8 8 5 11 4 7 2 2 0 0 0 10 7 11 2 12 6
Bud 5 11 32 4 6 4 – 4 4 4 6 4 7 6 2 1 0 0 2 1 2 3 5 7
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 1 5 7 4 4 2
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 20 95 65 81 58 45 – 45 33 53 55 39 92 124 100 41 18 17 11 51 77 23 46 41
Cytoplasm 62 113 87 85 118 158 – 254 161 171 208 189 107 116 115 40 45 16 29 102 39 33 69 74
Endoplasmic Reticulum 22 34 19 56 87 68 – 117 40 47 42 67 35 29 33 0 3 1 39 48 62 29 70 60
Endosome 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 2 1
Golgi 0 0 0 0 0 0 – 0 0 2 0 1 0 0 0 0 0 0 0 0 0 1 5 4
Mitochondria 0 1 1 2 0 2 – 2 6 9 13 7 1 0 0 0 1 0 1 0 1 0 2 1
Nucleus 0 0 0 0 0 0 – 0 0 0 1 1 0 0 2 0 0 0 0 0 0 1 3 1
Nuclear Periphery 0 0 0 1 0 1 – 0 1 0 0 0 0 0 0 0 0 0 2 0 0 0 1 0
Nucleolus 0 0 0 0 3 0 – 0 0 1 1 0 0 0 0 1 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 1 0 2 1 – 3 0 3 0 0 0 1 3 2 5 1 0 0 1 1 6 3
Unique Cell Count 89 193 170 182 223 236 386 215 247 279 276 187 197 200 68 53 24 102 221 206 103 237 215
Labelled Cell Count 113 259 230 246 280 280 433 253 295 337 312 249 278 257 85 73 35 102 221 206 103 237 215


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 24.4 30.2 19.9 26.0 28.5 29.2 – 27.9 27.3 25.9 24.6 24.7 35.6 40.8 42.6 58.8 56.0 57.2 34.6 34.8 34.9
Std Deviation (1e-4) 7.4 5.8 10.3 6.1 6.4 6.5 – 6.4 6.6 8.2 6.5 7.9 10.9 12.9 14.2 29.9 31.5 26.6 18.3 18.8 17.3
Intensity Change (Log2) – – – 0.39 0.52 0.56 – 0.49 0.46 0.38 0.31 0.31 0.84 1.04 1.1 1.56 1.49 1.52 0.8 0.81 0.81


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches -1.6 -4.4 -5.8 – -5.8 -3.8 -4.9 -4.1 -5.5 -3.6 -5.0 -5.1 -3.3 -3.0 0
Bud -5.1 -5.4 -6.0 – -7.9 -5.7 -6.1 -6.2 -6.5 -4.6 -4.9 -5.9 -3.5 -3.4 0
Bud Neck 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 1.2 -2.6 -4.3 – -7.2 -5.1 -3.7 -4.3 -5.8 2.1 4.7 2.3 3.1 -0.6 3.0
Cytoplasm -0.8 0.3 3.2 – 3.3 4.8 3.7 5.1 3.7 1.1 1.5 1.2 1.1 4.4 1.4
Endoplasmic Reticulum 4.5 6.2 4.3 – 4.8 2.0 2.2 1.2 3.4 2.0 1.0 1.5 -2.9 -1.2 0
Endosome 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 – 0 0 0 2.4 0 0 0 0 0 0 0
Nucleus 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 24.1915 27.2059 24.6365 24.6791 21.883 26.2416 24.1319 27.9316 24.1575 27.1053 22.6325 25.8192 26.3112 27.6178 26.0298 21.5911 26.5773 27.1041
Actin 0.046 0.0148 0.0374 0.0455 0.0652 0.0374 0.0338 0.0268 0.0534 0.014 0.0366 0.0296 0.0365 0.0203 0.0633 0.1057 0.0287 0.0583
Bud 0.0075 0.0009 0.0156 0.0105 0.0028 0.0017 0.0026 0.002 0.0152 0.0048 0.0045 0.0009 0.0035 0.0013 0.0176 0.0046 0.0012 0.0011
Bud Neck 0.0072 0.007 0.019 0.0157 0.0397 0.1173 0.0115 0.0061 0.0097 0.0291 0.0285 0.1324 0.0099 0.0063 0.0115 0.0105 0.0168 0.1399
Bud Periphery 0.0213 0.0022 0.0518 0.0433 0.0088 0.0121 0.0098 0.0056 0.0601 0.0462 0.0274 0.0079 0.0134 0.0026 0.0654 0.0309 0.0119 0.0097
Bud Site 0.0175 0.0988 0.0188 0.0087 0.0242 0.0014 0.012 0.0798 0.0284 0.0017 0.0023 0.0017 0.0146 0.1152 0.027 0.0087 0.0012 0.0011
Cell Periphery 0.1493 0.2263 0.2445 0.238 0.1241 0.1547 0.1905 0.281 0.2992 0.3255 0.2575 0.1759 0.1788 0.2338 0.2547 0.2146 0.1822 0.1109
Cytoplasm 0.4566 0.4727 0.3999 0.2363 0.4271 0.3823 0.3502 0.3813 0.3328 0.295 0.2958 0.2995 0.3884 0.4724 0.3692 0.2453 0.3352 0.3051
Cytoplasmic Foci 0.0054 0.0015 0.0073 0.0038 0.0451 0.0103 0.0151 0.0024 0.0059 0.0006 0.0023 0.0027 0.0081 0.0018 0.0037 0.0063 0.0176 0.0053
Eisosomes 0.0017 0.0014 0.001 0.001 0.0009 0.0015 0.0042 0.0018 0.0019 0.001 0.005 0.0017 0.0014 0.0014 0.002 0.0017 0.0025 0.0014
Endoplasmic Reticulum 0.2042 0.1387 0.1344 0.2821 0.1858 0.2431 0.2688 0.1659 0.1258 0.238 0.2656 0.3056 0.2634 0.117 0.1456 0.2075 0.3376 0.3285
Endosome 0.0105 0.0006 0.011 0.0165 0.0095 0.0028 0.0165 0.0013 0.0021 0.0004 0.0124 0.0016 0.0073 0.0008 0.0034 0.0147 0.0045 0.0077
Golgi 0.01 0.0031 0.0081 0.0253 0.011 0.0105 0.0122 0.0049 0.0048 0.0014 0.0164 0.0059 0.0078 0.0043 0.0043 0.013 0.0106 0.0115
Lipid Particles 0.0148 0.017 0.0142 0.0338 0.0238 0.0109 0.0259 0.0234 0.0172 0.0106 0.0179 0.0173 0.0151 0.0112 0.0132 0.0439 0.0165 0.0074
Mitochondria 0.0129 0.0001 0.0134 0.0116 0.0087 0.002 0.0075 0.0005 0.0125 0.0001 0.0053 0.0033 0.0125 0.0002 0.0014 0.0562 0.0006 0.0007
None 0.0036 0.0003 0.0006 0.0004 0.0014 0.0006 0.003 0.0002 0.0026 0.0002 0.0004 0.0003 0.009 0.0002 0.0015 0.0019 0.0209 0.0006
Nuclear Periphery 0.0116 0.0063 0.0075 0.0065 0.0033 0.004 0.0111 0.0057 0.0066 0.0053 0.0051 0.0046 0.0089 0.0051 0.0066 0.0083 0.0043 0.0048
Nucleolus 0.0007 0.0002 0.0004 0.0003 0.0002 0.0003 0.0009 0.0002 0.0011 0.0001 0.0002 0.0002 0.0003 0.0001 0.0001 0.0013 0.0002 0.0001
Nucleus 0.0023 0.001 0.0019 0.0015 0.0011 0.0012 0.0029 0.0009 0.002 0.001 0.001 0.0013 0.0018 0.001 0.0015 0.002 0.0007 0.0012
Peroxisomes 0.0013 0.0001 0.001 0.0005 0.0101 0.0003 0.002 0.0001 0.0051 0 0.0002 0.0002 0.0027 0.0001 0.0007 0.0063 0.0013 0.0003
Punctate Nuclear 0.0006 0.0002 0.0004 0.0003 0.0019 0.0003 0.0023 0.0002 0.0004 0.0001 0.0002 0.0002 0.0035 0.0002 0.0006 0.0035 0.0024 0.0008
Vacuole 0.0137 0.0069 0.0108 0.0168 0.005 0.0051 0.016 0.0096 0.0125 0.0243 0.0133 0.0067 0.012 0.0045 0.0065 0.0102 0.0026 0.0033
Vacuole Periphery 0.0013 0.0002 0.0008 0.0016 0.0004 0.0004 0.0012 0.0002 0.0006 0.0007 0.0021 0.0004 0.0011 0.0001 0.0004 0.0029 0.0003 0.0004

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 14.5533 7.0962 10.5314 26.1497 22.2997 37.0242 26.3083 34.7056 42.1303 38.6863
Translational Efficiency 2.6529 4.7676 3.4738 1.3144 1.5965 1.5426 2.1698 1.8704 1.4715 1.7445

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✔
Gene Expression ✔
Translational Efficiency ✘
Standard name
Human Ortholog
Description Mitochondrial ribosomal protein of the large subunit; appears as two protein spots (YmL34 and YmL38) on two-dimensional SDS gels; protein abundance increases in response to DNA replication stress
Localization
Cell Percentages ER (37%), cytoplasm (6%), mixed (56%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Mrpl38

Mrpl38


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Mrpl38-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available