Standard name
Human Ortholog
Description Vacuolar membrane zinc transporter; transports zinc from storage in the vacuole to the cytoplasm when needed; transcription is induced under conditions of zinc deficiency
DOWNLOAD

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Cytoplasm 0.61 0.77 0.9 0.87 0.64 0.74 0.79 0.87 – 0.8 0.86 0.81 0.93 0.99 1.0 – 0.63 0.57 0.15 0.23 0.2 0.2 0.18 0.19
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – 0.06 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Mitochondria 0.09 0.13 0 0 0.08 0.15 0.13 0.13 – 0.23 0.21 0.21 0 0 0 – 0 0 0 0 0 0 0 0.05
Nucleus 0.07 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – 0.11 0.08 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.34 0.18 0.17 0.23 0.42 0.35 0.3 0.16 – 0.18 0.14 0.18 0.12 0 0 – 0.42 0.51 0.76 0.68 0.67 0.72 0.7 0.67
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – 0 0 1 0 1 1 3 4
Bud 0 3 0 1 0 1 4 5 – 2 7 4 0 0 0 – 0 0 1 2 6 2 9 8
Bud Neck 0 0 1 0 0 0 0 1 – 0 0 0 0 2 1 – 0 0 0 0 2 1 1 1
Bud Site 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – 0 0 – – – – – –
Cell Periphery 0 0 0 0 1 0 0 1 – 0 1 0 4 0 0 – 0 0 0 0 0 0 0 0
Cytoplasm 98 172 422 288 202 363 538 573 – 495 542 558 381 451 509 – 373 247 32 60 98 96 105 120
Endoplasmic Reticulum 2 4 2 0 0 0 0 0 – 1 0 1 1 1 0 – 35 19 1 0 6 6 6 7
Endosome 1 0 4 0 7 0 0 3 – 0 0 0 2 1 0 – 12 9 4 6 9 4 12 10
Golgi 1 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – 0 0 0 0 2 0 1 0
Mitochondria 14 29 8 7 24 72 88 86 – 142 131 147 0 0 0 – 21 11 1 7 20 11 23 31
Nucleus 12 8 2 0 7 16 7 11 – 14 8 4 4 3 1 – 63 34 1 2 0 1 1 1
Nuclear Periphery 3 0 0 2 0 3 12 3 – 16 2 8 0 0 0 – 0 0 1 1 1 0 1 1
Nucleolus 0 0 2 0 1 0 0 1 – 1 2 3 0 0 0 – 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 1 0 – 0 0 0 0 0 0 – 0 0 0 0 1 0 0 0
SpindlePole 1 0 0 0 1 0 1 0 – 1 1 0 0 0 0 – 1 0 4 1 0 1 5 8
Vac/Vac Membrane 55 39 80 75 133 171 207 107 – 109 91 123 50 7 2 – 253 221 164 179 325 342 421 426
Unique Cell Count 161 222 467 331 315 492 682 661 621 628 689 408 457 511 596 436 218 264 487 476 602 633
Labelled Cell Count 187 255 521 373 376 626 858 791 781 785 848 442 465 513 758 541 218 264 487 476 602 633


Vacuole

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.4 4.0 3.9 4.3 4.1 3.7 3.7 3.8 – 3.3 3.4 3.4 5.6 5.4 5.6 4.8 5.4 5.8 5.3 5.5 5.7
Std Deviation (1e-4) 0.7 0.6 0.9 1.5 1.4 1.2 1.5 1.0 – 1.0 0.7 0.8 1.0 1.1 1.2 1.1 1.3 1.3 1.2 1.3 1.6
Intensity Change (Log2) – – – 0.15 0.06 -0.08 -0.07 -0.05 – -0.26 -0.18 -0.2 0.52 0.47 0.51 0.29 0.46 0.56 0.43 0.49 0.56


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – 0 0
Bud Neck – – – – – – – – – – – – – 0 0
Bud Site – – – – – – – – – – – – – 0 0
Cell Periphery – – – – – – – – – – – – – 0 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – 0 0
Endosome – – – – – – – – – – – – – 0 0
Golgi – – – – – – – – – – – – – 0 0
Mitochondria – – – – – – – – – – – – – 0 0
Nuclear Periphery – – – – – – – – – – – – – 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – 0 0
Peroxisomes – – – – – – – – – – – – – 0 0
SpindlePole – – – – – – – – – – – – – 0 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – 0 0
Cytoplasm – – – – – – – – – – – – – 0 0
Nucleus – – – – – – – – – – – – – 0 0
Vacuole – – – – – – – – – – – – – 8.8 10.7

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.5909 2.1627 2.1861 2.0109 1.9859 2.0655 2.7692 3.5592 3.4549 3.6674 3.9681 3.4114 2.1668 3.4166 3.0915 3.2682 2.727 3.5953
Actin 0.0024 0.0008 0.0087 0.0086 0.0009 0.0002 0.0047 0.0002 0.0076 0.0299 0.0188 0.0015 0.0001 0.0001 0.0001 0 0 0.0001
Bud 0.0008 0.0019 0.0007 0.013 0.0005 0.0005 0.0018 0.0039 0.0009 0.0007 0.0012 0.002 0.0003 0.0007 0.0005 0.0002 0.0003 0.0006
Bud Neck 0.022 0.0016 0.0012 0.0007 0.0013 0.0016 0.0025 0.0009 0.0021 0.0184 0.0055 0.003 0.0003 0.0013 0.0005 0.0007 0.0005 0.0013
Bud Periphery 0.002 0.0023 0.0013 0.015 0.0011 0.001 0.0033 0.002 0.002 0.0016 0.0027 0.0015 0.0005 0.0009 0.0007 0.0003 0.0004 0.0013
Bud Site 0.0053 0.0093 0.0014 0.0022 0.0015 0.0009 0.0043 0.0096 0.0035 0.0017 0.0017 0.0011 0.0009 0.0029 0.0012 0.0007 0.0004 0.0006
Cell Periphery 0.0013 0.001 0.0007 0.0006 0.0006 0.0009 0.0009 0.0008 0.0008 0.001 0.0006 0.0006 0.001 0.0009 0.0005 0.0006 0.001 0.0008
Cytoplasm 0.1197 0.1386 0.1145 0.1243 0.0901 0.1045 0.0994 0.1085 0.1312 0.0902 0.0717 0.1674 0.0975 0.1712 0.1628 0.0793 0.1783 0.101
Cytoplasmic Foci 0.0699 0.0335 0.0659 0.0249 0.0515 0.0411 0.0621 0.0312 0.0411 0.0213 0.0443 0.0598 0.0225 0.0292 0.0301 0.0488 0.0248 0.036
Eisosomes 0.0003 0.0001 0.0001 0 0 0 0.0001 0 0.0002 0.002 0.0002 0 0.0001 0.0001 0.0001 0 0 0
Endoplasmic Reticulum 0.0046 0.0072 0.0053 0.0044 0.0015 0.0054 0.0129 0.007 0.0103 0.0043 0.0104 0.0052 0.0063 0.0055 0.0067 0.0021 0.0045 0.0044
Endosome 0.2275 0.165 0.2383 0.2202 0.1908 0.2253 0.2578 0.1436 0.1936 0.2307 0.2495 0.1855 0.1619 0.1184 0.1847 0.3788 0.1991 0.2342
Golgi 0.0068 0.0028 0.006 0.0024 0.0023 0.0037 0.0063 0.0016 0.0038 0.004 0.0062 0.0019 0.0011 0.0016 0.003 0.0035 0.001 0.0024
Lipid Particles 0.0153 0.0056 0.014 0.0021 0.0057 0.0057 0.0267 0.0034 0.0061 0.003 0.0061 0.0041 0.0053 0.005 0.0081 0.0027 0.0017 0.0047
Mitochondria 0.0069 0.0038 0.0216 0.0069 0.0031 0.0025 0.0105 0.0028 0.0127 0.0066 0.0063 0.0039 0.0018 0.0017 0.0122 0.0013 0.0008 0.003
None 0.1972 0.1608 0.2292 0.1315 0.1715 0.1428 0.1559 0.2134 0.2301 0.1898 0.1056 0.1291 0.2274 0.3066 0.2461 0.0155 0.1227 0.1074
Nuclear Periphery 0.0041 0.0115 0.015 0.0039 0.0109 0.0072 0.0195 0.0066 0.018 0.0088 0.027 0.0064 0.0069 0.0053 0.0076 0.0026 0.0036 0.0087
Nucleolus 0.0046 0.0084 0.003 0.0014 0.004 0.0026 0.0026 0.0022 0.0024 0.0022 0.0035 0.0015 0.0016 0.0028 0.0013 0.0017 0.0009 0.0016
Nucleus 0.0069 0.0205 0.0086 0.0042 0.0081 0.0127 0.0083 0.0084 0.0064 0.0161 0.0125 0.0105 0.0106 0.0096 0.0198 0.0029 0.003 0.0142
Peroxisomes 0.0063 0.0007 0.0097 0.0015 0.0025 0.0014 0.0078 0.0027 0.0019 0.001 0.0023 0.0008 0.0002 0.0005 0.0008 0.0005 0.0002 0.0005
Punctate Nuclear 0.0029 0.0022 0.0018 0.0006 0.0208 0.0023 0.0099 0.0027 0.0035 0.0009 0.0112 0.0039 0.0013 0.0034 0.0007 0.0004 0.0006 0.0007
Vacuole 0.2651 0.3953 0.2258 0.3933 0.3967 0.3981 0.2774 0.4138 0.285 0.3278 0.3629 0.3719 0.4013 0.306 0.2752 0.4246 0.4026 0.4221
Vacuole Periphery 0.0282 0.0271 0.0271 0.0385 0.0346 0.0393 0.0254 0.0347 0.0368 0.0382 0.0497 0.0382 0.051 0.0265 0.0376 0.0327 0.0537 0.0542

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 62.03 61.3223 67.2876 59.7934 58.1896 66.3718 83.7699 86.3668 73.5499 74.7248
Translational Efficiency 1.0009 1.0483 0.8779 0.7783 0.7278 0.8195 0.8352 0.7124 0.7928 0.6639

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘
Standard name
Human Ortholog
Description Vacuolar membrane zinc transporter; transports zinc from storage in the vacuole to the cytoplasm when needed; transcription is induced under conditions of zinc deficiency
Localization
Cell Percentages vacuole (81%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Zrt3

Zrt3


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Zrt3-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available