Standard name | |
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Human Ortholog | |
Description | Golgi membrane protein with similarity to mammalian CASP; genetic interactions with GOS1 (encoding a Golgi snare protein) suggest a role in Golgi function |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 9 | 37 | 7 | 15 | 11 | 1 | 0 | – | 0 | 1 | 1 | 1 | 22 | 11 | 2 | 18 | 7 | 6 | 21 | 37 | 13 | 17 | 21 | 29 |
Bud | 4 | 11 | 0 | 1 | 2 | 0 | 1 | – | 2 | 4 | 6 | 4 | 3 | 3 | 0 | 1 | 1 | 0 | 1 | 4 | 0 | 2 | 2 | 6 |
Bud Neck | 0 | 0 | 1 | 0 | 0 | 0 | 0 | – | 1 | 4 | 0 | 1 | 0 | 0 | 1 | 1 | 0 | 0 | 3 | 3 | 1 | 5 | 1 | 6 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 2 | 2 | 1 | 1 | 2 | 0 | 2 | – | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 37 | 41 | 32 | 48 | 109 | 77 | 126 | – | 94 | 119 | 81 | 105 | 26 | 50 | 47 | 37 | 24 | 26 | 13 | 9 | 15 | 22 | 25 | 30 |
Endoplasmic Reticulum | 65 | 59 | 19 | 26 | 18 | 7 | 3 | – | 4 | 2 | 6 | 11 | 60 | 33 | 14 | 9 | 6 | 8 | 32 | 42 | 18 | 16 | 20 | 31 |
Endosome | 31 | 43 | 12 | 34 | 24 | 14 | 13 | – | 7 | 7 | 6 | 4 | 44 | 100 | 64 | 77 | 89 | 95 | 75 | 69 | 26 | 46 | 43 | 65 |
Golgi | 0 | 3 | 0 | 0 | 1 | 0 | 0 | – | 0 | 1 | 0 | 0 | 2 | 1 | 0 | 9 | 11 | 4 | 14 | 35 | 6 | 6 | 11 | 19 |
Mitochondria | 27 | 4 | 0 | 5 | 1 | 3 | 19 | – | 46 | 60 | 149 | 108 | 0 | 1 | 1 | 13 | 10 | 3 | 31 | 19 | 29 | 35 | 40 | 39 |
Nucleus | 0 | 0 | 1 | 2 | 1 | 3 | 1 | – | 0 | 1 | 2 | 7 | 2 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 2 |
Nuclear Periphery | 1 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 1 | 2 | 1 |
Nucleolus | 0 | 0 | 0 | 1 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 2 | 1 | 1 | 0 | 1 | 1 | 1 | – | 0 | 0 | 0 | 0 | 1 | 5 | 1 | 0 | 0 | 1 | 7 | 2 | 0 | 5 | 5 | 5 |
SpindlePole | 14 | 5 | 1 | 11 | 8 | 1 | 2 | – | 3 | 8 | 3 | 8 | 26 | 27 | 10 | 29 | 23 | 25 | 6 | 2 | 2 | 10 | 6 | 6 |
Vac/Vac Membrane | 0 | 15 | 5 | 3 | 6 | 4 | 5 | – | 5 | 6 | 6 | 6 | 1 | 7 | 5 | 19 | 19 | 23 | 10 | 7 | 11 | 11 | 11 | 13 |
Unique Cell Count | 158 | 174 | 70 | 119 | 165 | 103 | 158 | 153 | 191 | 223 | 226 | 150 | 191 | 131 | 174 | 156 | 150 | 217 | 239 | 131 | 188 | 198 | 263 | |
Labelled Cell Count | 192 | 221 | 80 | 147 | 184 | 111 | 173 | 163 | 213 | 261 | 257 | 187 | 239 | 147 | 217 | 190 | 192 | 217 | 239 | 131 | 188 | 198 | 263 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 5.3 | 8.2 | 7.5 | 7.6 | 7.3 | 6.2 | 5.8 | – | 4.7 | 5.1 | 4.4 | 4.6 | 8.3 | 8.2 | 7.2 | 8.5 | 9.5 | 8.5 | 7.4 | 7.3 | 8.0 |
Std Deviation (1e-4) | 0.7 | 1.1 | 1.2 | 1.8 | 1.6 | 2.3 | 1.8 | – | 1.1 | 1.7 | 1.6 | 1.5 | 1.4 | 1.7 | 1.3 | 1.4 | 2.0 | 1.7 | 1.1 | 1.2 | 1.6 |
Intensity Change (Log2) | – | – | – | 0.03 | -0.04 | -0.26 | -0.37 | – | -0.66 | -0.54 | -0.77 | -0.7 | 0.16 | 0.13 | -0.06 | 0.18 | 0.34 | 0.19 | -0.01 | -0.03 | 0.09 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0.5 | -0.9 | 0 | 0 | – | 0 | 0 | 0 | 0 | 1.0 | 0 | 0 | 0.1 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | -0.7 | 2.9 | 3.9 | 5.1 | – | 2.2 | 2.4 | -1.4 | 0.1 | -4.5 | -3.0 | -1.4 | -3.8 | -4.9 | -4.5 |
Endoplasmic Reticulum | -0.8 | -3.1 | -3.7 | -6.0 | – | 0 | -6.9 | 0 | 0 | 1.9 | -1.8 | -3.0 | -4.9 | -5.2 | -4.6 |
Endosome | 1.8 | -0.5 | -0.6 | -2.0 | – | 0 | -3.7 | 0 | 0 | 1.9 | 5.1 | 4.4 | 4.0 | 5.6 | 6.4 |
Golgi | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 3.0 | – | 0 | 5.3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 3.3 | 2.9 | 0 | 3.3 | 3.0 | 3.3 |
Vacuole | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0.9 | 1.1 | 1.7 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 2.3255 | 3.5745 | 2.8705 | 2.5065 | 2.5836 | 3.3056 | 2.7665 | 3.9676 | 3.6213 | 3.3332 | 3.1562 | 3.5994 | 3.9006 | 5.3202 | 4.4827 | 4.4032 | 4.1636 | 4.5308 |
Actin | 0.0234 | 0.0021 | 0.0384 | 0.029 | 0.003 | 0.0342 | 0.0088 | 0.0093 | 0.0097 | 0.0093 | 0.0106 | 0.0047 | 0.0035 | 0.0033 | 0.0033 | 0.0018 | 0.001 | 0.0009 |
Bud | 0.0004 | 0.0001 | 0.0007 | 0.0009 | 0.0003 | 0.0002 | 0.0003 | 0.0002 | 0.0004 | 0.0004 | 0.0004 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0.0003 | 0 | 0.0001 |
Bud Neck | 0.0023 | 0.0014 | 0.0026 | 0.0016 | 0.0007 | 0.0012 | 0.0009 | 0.001 | 0.0046 | 0.0028 | 0.0009 | 0.0008 | 0.0003 | 0.0006 | 0.0007 | 0.0004 | 0.0003 | 0.0004 |
Bud Periphery | 0.0006 | 0.0001 | 0.0006 | 0.001 | 0.0006 | 0.0002 | 0.0005 | 0.0002 | 0.0003 | 0.0005 | 0.0005 | 0.0003 | 0.0001 | 0.0002 | 0.0001 | 0.001 | 0 | 0.0001 |
Bud Site | 0.0054 | 0.0031 | 0.016 | 0.0044 | 0.0005 | 0.0006 | 0.0047 | 0.0095 | 0.0045 | 0.0023 | 0.0013 | 0.0003 | 0.0003 | 0.008 | 0.0016 | 0.0002 | 0.0002 | 0.0002 |
Cell Periphery | 0.0006 | 0.0003 | 0.0005 | 0.0004 | 0.0003 | 0.0003 | 0.0003 | 0.0008 | 0.0004 | 0.0002 | 0.0002 | 0.0002 | 0.0001 | 0.0002 | 0.0002 | 0.0001 | 0.0001 | 0.0001 |
Cytoplasm | 0.0067 | 0.0767 | 0.0219 | 0.0056 | 0.0023 | 0.0045 | 0.0062 | 0.013 | 0.0214 | 0.0069 | 0.0016 | 0.0051 | 0.0063 | 0.0114 | 0.0131 | 0.0375 | 0.0032 | 0.0054 |
Cytoplasmic Foci | 0.1634 | 0.1561 | 0.179 | 0.1022 | 0.0993 | 0.0972 | 0.1343 | 0.1458 | 0.1555 | 0.1875 | 0.1183 | 0.1222 | 0.1179 | 0.1175 | 0.1346 | 0.1107 | 0.1483 | 0.1058 |
Eisosomes | 0.0002 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.0142 | 0.0197 | 0.008 | 0.0776 | 0.0067 | 0.0243 | 0.0041 | 0.0168 | 0.0127 | 0.0019 | 0.0013 | 0.0089 | 0.0129 | 0.0179 | 0.0083 | 0.0109 | 0.0017 | 0.0117 |
Endosome | 0.1989 | 0.2562 | 0.1911 | 0.3465 | 0.2639 | 0.1983 | 0.2011 | 0.2085 | 0.2106 | 0.2277 | 0.1823 | 0.1931 | 0.2889 | 0.2603 | 0.3017 | 0.3019 | 0.2803 | 0.2291 |
Golgi | 0.0998 | 0.0971 | 0.1319 | 0.1263 | 0.1916 | 0.1256 | 0.0918 | 0.083 | 0.1057 | 0.1392 | 0.1218 | 0.1096 | 0.0885 | 0.1117 | 0.1411 | 0.1649 | 0.1846 | 0.1024 |
Lipid Particles | 0.4004 | 0.3238 | 0.3437 | 0.2343 | 0.1768 | 0.4779 | 0.4712 | 0.4609 | 0.423 | 0.3217 | 0.4503 | 0.5085 | 0.4446 | 0.4356 | 0.3573 | 0.3129 | 0.3111 | 0.5094 |
Mitochondria | 0.0144 | 0.0026 | 0.0196 | 0.0402 | 0.1326 | 0.0025 | 0.0126 | 0.0134 | 0.0048 | 0.0313 | 0.0528 | 0.0059 | 0.0049 | 0.0031 | 0.0034 | 0.0036 | 0.0017 | 0.0037 |
None | 0.0062 | 0.016 | 0.0011 | 0.0002 | 0.0006 | 0.0002 | 0.0162 | 0.0017 | 0.0086 | 0.0003 | 0.0002 | 0.0012 | 0.0005 | 0.0007 | 0.0002 | 0.0016 | 0.0001 | 0.0003 |
Nuclear Periphery | 0.0063 | 0.0038 | 0.0013 | 0.003 | 0.0215 | 0.0086 | 0.0052 | 0.0014 | 0.0019 | 0.0006 | 0.001 | 0.0056 | 0.0083 | 0.0076 | 0.0052 | 0.0089 | 0.0005 | 0.0033 |
Nucleolus | 0.0006 | 0.0011 | 0.0001 | 0.0002 | 0.0002 | 0.0001 | 0.0005 | 0.0004 | 0.0004 | 0.0005 | 0.0002 | 0.0001 | 0.0002 | 0.0005 | 0.0001 | 0.0001 | 0.0001 | 0.0002 |
Nucleus | 0.0009 | 0.0019 | 0.0002 | 0.0003 | 0.0013 | 0.0002 | 0.0009 | 0.0005 | 0.0006 | 0.0002 | 0.0002 | 0.0002 | 0.0005 | 0.0006 | 0.0007 | 0.0008 | 0.0001 | 0.0003 |
Peroxisomes | 0.0363 | 0.0254 | 0.0359 | 0.01 | 0.0668 | 0.0127 | 0.0258 | 0.0211 | 0.0247 | 0.0598 | 0.0504 | 0.0261 | 0.0125 | 0.0096 | 0.0173 | 0.0143 | 0.061 | 0.0174 |
Punctate Nuclear | 0.0056 | 0.0065 | 0.0018 | 0.0007 | 0.0008 | 0.0015 | 0.0085 | 0.0019 | 0.0021 | 0.0018 | 0.001 | 0.0019 | 0.0025 | 0.0017 | 0.0039 | 0.0017 | 0.0008 | 0.0021 |
Vacuole | 0.0082 | 0.0043 | 0.0044 | 0.0116 | 0.0116 | 0.007 | 0.0037 | 0.0096 | 0.0065 | 0.0033 | 0.0025 | 0.0034 | 0.0051 | 0.0073 | 0.0054 | 0.0151 | 0.0035 | 0.0053 |
Vacuole Periphery | 0.0053 | 0.0014 | 0.0013 | 0.0039 | 0.0185 | 0.0028 | 0.0023 | 0.0009 | 0.0015 | 0.0018 | 0.0022 | 0.0016 | 0.0017 | 0.0019 | 0.0015 | 0.0114 | 0.0013 | 0.0017 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 27.3147 | 21.2748 | 22.245 | 27.091 | 22.7916 | 21.6234 | 23.222 | 28.2119 | 32.9837 | 29.0437 |
Translational Efficiency | 1.2277 | 1.412 | 1.147 | 1.2542 | 1.2645 | 1.3958 | 1.3748 | 1.1087 | 1.0358 | 1.1431 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✔ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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