Standard name
Human Ortholog
Description Golgi membrane protein with similarity to mammalian CASP; genetic interactions with GOS1 (encoding a Golgi snare protein) suggest a role in Golgi function

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.06 0.21 0.1 0.13 0.07 0 0 0 0 0 0 0.15 0.06 0 0.1 0 0 0.1 0.16 0.1 0.09 0.11 0.11
Bud 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.23 0.24 0.46 0.4 0.66 0.75 0.8 0.61 0.62 0.36 0.46 0.17 0.26 0.36 0.21 0.15 0.17 0.06 0 0.12 0.12 0.13 0.12
Endoplasmic Reticulum 0.41 0.34 0.27 0.22 0.11 0.07 0 0 0 0 0.05 0.4 0.17 0.11 0.05 0 0.05 0.15 0.18 0.14 0.09 0.1 0.12
Endosome 0.2 0.25 0.17 0.29 0.15 0.14 0.08 0.05 0 0 0 0.29 0.52 0.49 0.44 0.57 0.63 0.35 0.29 0.2 0.25 0.22 0.25
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.07 0 0.07 0.15 0.05 0 0.06 0.07
Mitochondria 0.17 0 0 0 0 0 0.12 0.3 0.31 0.67 0.48 0 0 0 0.07 0.06 0 0.14 0.08 0.22 0.19 0.21 0.15
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0.09 0 0 0.09 0.05 0 0 0 0 0 0 0.17 0.14 0.08 0.17 0.15 0.17 0 0 0 0.05 0 0
Vac/Vac Membrane 0 0.09 0.07 0 0 0 0 0 0 0 0 0 0 0 0.11 0.12 0.15 0.05 0 0.08 0.06 0.06 0.05
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 9 37 7 15 11 1 0 0 1 1 1 22 11 2 18 7 6 21 37 13 17 21 29
Bud 4 11 0 1 2 0 1 2 4 6 4 3 3 0 1 1 0 1 4 0 2 2 6
Bud Neck 0 0 1 0 0 0 0 1 4 0 1 0 0 1 1 0 0 3 3 1 5 1 6
Bud Site 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0
Cell Periphery 2 2 1 1 2 0 2 1 0 0 0 0 0 0 1 0 1 0 0 0 0 0 0
Cytoplasm 37 41 32 48 109 77 126 94 119 81 105 26 50 47 37 24 26 13 9 15 22 25 30
Endoplasmic Reticulum 65 59 19 26 18 7 3 4 2 6 11 60 33 14 9 6 8 32 42 18 16 20 31
Endosome 31 43 12 34 24 14 13 7 7 6 4 44 100 64 77 89 95 75 69 26 46 43 65
Golgi 0 3 0 0 1 0 0 0 1 0 0 2 1 0 9 11 4 14 35 6 6 11 19
Mitochondria 27 4 0 5 1 3 19 46 60 149 108 0 1 1 13 10 3 31 19 29 35 40 39
Nucleus 0 0 1 2 1 3 1 0 1 2 7 2 1 1 1 0 0 0 0 0 1 0 2
Nuclear Periphery 1 0 0 0 0 0 0 0 0 1 1 0 0 0 1 0 0 0 1 0 1 2 1
Nucleolus 0 0 0 1 0 0 0 0 0 0 0 0 0 1 1 0 0 0 0 0 0 0 0
Peroxisomes 2 1 1 0 1 1 1 0 0 0 0 1 5 1 0 0 1 7 2 0 5 5 5
SpindlePole 14 5 1 11 8 1 2 3 8 3 8 26 27 10 29 23 25 6 2 2 10 6 6
Vac/Vac Membrane 0 15 5 3 6 4 5 5 6 6 6 1 7 5 19 19 23 10 7 11 11 11 13
Unique Cell Count 158 174 70 119 165 103 158 153 191 223 226 150 191 131 174 156 150 217 239 131 188 198 263
Labelled Cell Count 192 221 80 147 184 111 173 163 213 261 257 187 239 147 217 190 192 217 239 131 188 198 263


Lipid Particles

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.3 8.2 7.5 7.6 7.3 6.2 5.8 4.7 5.1 4.4 4.6 8.3 8.2 7.2 8.5 9.5 8.5 7.4 7.3 8.0
Std Deviation (1e-4) 0.7 1.1 1.2 1.8 1.6 2.3 1.8 1.1 1.7 1.6 1.5 1.4 1.7 1.3 1.4 2.0 1.7 1.1 1.2 1.6
Intensity Change (Log2) 0.03 -0.04 -0.26 -0.37 -0.66 -0.54 -0.77 -0.7 0.16 0.13 -0.06 0.18 0.34 0.19 -0.01 -0.03 0.09


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0.5 -0.9 0 0 0 0 0 0 1.0 0 0 0.1 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -0.7 2.9 3.9 5.1 2.2 2.4 -1.4 0.1 -4.5 -3.0 -1.4 -3.8 -4.9 -4.5
Endoplasmic Reticulum -0.8 -3.1 -3.7 -6.0 0 -6.9 0 0 1.9 -1.8 -3.0 -4.9 -5.2 -4.6
Endosome 1.8 -0.5 -0.6 -2.0 0 -3.7 0 0 1.9 5.1 4.4 4.0 5.6 6.4
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 3.0 0 5.3 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 3.3 2.9 0 3.3 3.0 3.3
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0.9 1.1 1.7

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.3255 3.5745 2.8705 2.5065 2.5836 3.3056 2.7665 3.9676 3.6213 3.3332 3.1562 3.5994 3.9006 5.3202 4.4827 4.4032 4.1636 4.5308
Actin 0.0234 0.0021 0.0384 0.029 0.003 0.0342 0.0088 0.0093 0.0097 0.0093 0.0106 0.0047 0.0035 0.0033 0.0033 0.0018 0.001 0.0009
Bud 0.0004 0.0001 0.0007 0.0009 0.0003 0.0002 0.0003 0.0002 0.0004 0.0004 0.0004 0.0001 0.0001 0.0002 0.0001 0.0003 0 0.0001
Bud Neck 0.0023 0.0014 0.0026 0.0016 0.0007 0.0012 0.0009 0.001 0.0046 0.0028 0.0009 0.0008 0.0003 0.0006 0.0007 0.0004 0.0003 0.0004
Bud Periphery 0.0006 0.0001 0.0006 0.001 0.0006 0.0002 0.0005 0.0002 0.0003 0.0005 0.0005 0.0003 0.0001 0.0002 0.0001 0.001 0 0.0001
Bud Site 0.0054 0.0031 0.016 0.0044 0.0005 0.0006 0.0047 0.0095 0.0045 0.0023 0.0013 0.0003 0.0003 0.008 0.0016 0.0002 0.0002 0.0002
Cell Periphery 0.0006 0.0003 0.0005 0.0004 0.0003 0.0003 0.0003 0.0008 0.0004 0.0002 0.0002 0.0002 0.0001 0.0002 0.0002 0.0001 0.0001 0.0001
Cytoplasm 0.0067 0.0767 0.0219 0.0056 0.0023 0.0045 0.0062 0.013 0.0214 0.0069 0.0016 0.0051 0.0063 0.0114 0.0131 0.0375 0.0032 0.0054
Cytoplasmic Foci 0.1634 0.1561 0.179 0.1022 0.0993 0.0972 0.1343 0.1458 0.1555 0.1875 0.1183 0.1222 0.1179 0.1175 0.1346 0.1107 0.1483 0.1058
Eisosomes 0.0002 0.0002 0.0001 0.0001 0.0001 0.0001 0.0002 0.0001 0.0001 0.0001 0.0001 0 0.0001 0 0 0 0 0
Endoplasmic Reticulum 0.0142 0.0197 0.008 0.0776 0.0067 0.0243 0.0041 0.0168 0.0127 0.0019 0.0013 0.0089 0.0129 0.0179 0.0083 0.0109 0.0017 0.0117
Endosome 0.1989 0.2562 0.1911 0.3465 0.2639 0.1983 0.2011 0.2085 0.2106 0.2277 0.1823 0.1931 0.2889 0.2603 0.3017 0.3019 0.2803 0.2291
Golgi 0.0998 0.0971 0.1319 0.1263 0.1916 0.1256 0.0918 0.083 0.1057 0.1392 0.1218 0.1096 0.0885 0.1117 0.1411 0.1649 0.1846 0.1024
Lipid Particles 0.4004 0.3238 0.3437 0.2343 0.1768 0.4779 0.4712 0.4609 0.423 0.3217 0.4503 0.5085 0.4446 0.4356 0.3573 0.3129 0.3111 0.5094
Mitochondria 0.0144 0.0026 0.0196 0.0402 0.1326 0.0025 0.0126 0.0134 0.0048 0.0313 0.0528 0.0059 0.0049 0.0031 0.0034 0.0036 0.0017 0.0037
None 0.0062 0.016 0.0011 0.0002 0.0006 0.0002 0.0162 0.0017 0.0086 0.0003 0.0002 0.0012 0.0005 0.0007 0.0002 0.0016 0.0001 0.0003
Nuclear Periphery 0.0063 0.0038 0.0013 0.003 0.0215 0.0086 0.0052 0.0014 0.0019 0.0006 0.001 0.0056 0.0083 0.0076 0.0052 0.0089 0.0005 0.0033
Nucleolus 0.0006 0.0011 0.0001 0.0002 0.0002 0.0001 0.0005 0.0004 0.0004 0.0005 0.0002 0.0001 0.0002 0.0005 0.0001 0.0001 0.0001 0.0002
Nucleus 0.0009 0.0019 0.0002 0.0003 0.0013 0.0002 0.0009 0.0005 0.0006 0.0002 0.0002 0.0002 0.0005 0.0006 0.0007 0.0008 0.0001 0.0003
Peroxisomes 0.0363 0.0254 0.0359 0.01 0.0668 0.0127 0.0258 0.0211 0.0247 0.0598 0.0504 0.0261 0.0125 0.0096 0.0173 0.0143 0.061 0.0174
Punctate Nuclear 0.0056 0.0065 0.0018 0.0007 0.0008 0.0015 0.0085 0.0019 0.0021 0.0018 0.001 0.0019 0.0025 0.0017 0.0039 0.0017 0.0008 0.0021
Vacuole 0.0082 0.0043 0.0044 0.0116 0.0116 0.007 0.0037 0.0096 0.0065 0.0033 0.0025 0.0034 0.0051 0.0073 0.0054 0.0151 0.0035 0.0053
Vacuole Periphery 0.0053 0.0014 0.0013 0.0039 0.0185 0.0028 0.0023 0.0009 0.0015 0.0018 0.0022 0.0016 0.0017 0.0019 0.0015 0.0114 0.0013 0.0017

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 27.3147 21.2748 22.245 27.091 22.7916 21.6234 23.222 28.2119 32.9837 29.0437
Translational Efficiency 1.2277 1.412 1.147 1.2542 1.2645 1.3958 1.3748 1.1087 1.0358 1.1431

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency
Standard name
Human Ortholog
Description Golgi membrane protein with similarity to mammalian CASP; genetic interactions with GOS1 (encoding a Golgi snare protein) suggest a role in Golgi function
Localization
Cell Percentages ER (30%)
Cell Cycle Regulation No
Subcompartmental Group ER-2

Coy1

Coy1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Coy1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available