Standard name
Human Ortholog
Description eIF4E-associated protein, competes with eIF4G for binding to eIF4E; accelerates mRNA degradation by promoting decapping, facilitated by interaction with eIF4E; essential for Puf5p mediated repression; associates with Puf5p and Dhh1p; inhibits cap-dependent translation; functions independently of eIF4E to maintain genetic stability; plays a role in cell growth, implicated in the TOR signaling cascade
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0.05 0 0 0.07 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0.05 0 0.06 0.08 0 0 0 0.07 0.09 0.12 0 0 0 0 0 0
Cytoplasm 0.97 0.99 0.98 0.96 0.93 0.89 0.77 – 0.65 0.69 0.62 0.54 0.98 0.99 0.98 0.89 0.91 0.86 0.93 0.94 0.89 0.95 0.94 0.93
Endoplasmic Reticulum 0 0 0 0 0.07 0.08 0.14 – 0.15 0.14 0.07 0.13 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0.06 – 0.15 0.12 0.36 0.25 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 – 0.06 0.07 0 0.09 0 0 0 0.07 0.06 0.09 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 1 4 13 – 15 8 7 18 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 1 0 3 – 0 1 0 2 1 0 0 1 0 0 0 1 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 1 13 4 6 9 6 9 – 15 10 12 22 3 6 12 18 24 27 0 0 0 0 0 0
Cytoplasm 119 340 121 170 222 205 240 – 183 187 133 144 131 313 315 227 230 195 118 326 120 133 298 297
Endoplasmic Reticulum 2 8 1 6 16 18 43 – 41 38 15 35 2 3 4 7 6 4 3 5 4 0 5 4
Endosome 0 0 0 0 0 0 1 – 2 0 0 1 0 0 1 0 1 0 0 2 0 0 1 1
Golgi 0 0 0 0 0 2 5 – 3 2 0 10 0 0 1 3 0 2 1 1 0 0 0 1
Mitochondria 1 1 0 1 1 7 18 – 41 33 77 66 0 0 0 2 1 3 0 1 2 2 3 3
Nucleus 0 1 0 2 2 0 1 – 2 2 0 0 1 1 0 7 3 2 0 1 0 0 0 1
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 1 1 0 0 0 0 0 0 0 0 1 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Vac/Vac Membrane 1 0 4 1 2 2 12 – 18 18 8 25 1 0 0 19 15 21 0 4 3 0 4 2
Unique Cell Count 123 345 124 177 239 230 311 280 271 215 268 134 315 320 255 254 226 127 346 136 140 317 319
Labelled Cell Count 124 363 130 186 254 244 345 320 299 253 324 139 323 333 284 280 254 127 346 136 140 317 319


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 10.9 15.0 12.8 12.0 12.9 11.3 11.1 – 10.0 10.4 9.2 10.2 11.8 12.5 12.4 18.8 18.2 17.3 11.2 11.9 12.6
Std Deviation (1e-4) 1.6 2.1 1.8 2.0 2.1 1.6 1.6 – 1.7 2.1 1.8 2.0 1.7 1.5 1.6 4.5 4.1 4.6 1.9 1.7 1.8
Intensity Change (Log2) – – – -0.09 0.02 -0.18 -0.21 – -0.36 -0.3 -0.48 -0.32 -0.11 -0.03 -0.04 0.55 0.51 0.44 -0.19 -0.1 -0.02


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 – 0 0 0 3.0 0 0 0 0 0 0
Bud 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 – 0.9 0 1.0 1.8 0 0 0 1.5 2.2 2.7
Cytoplasm -0.7 -1.9 -2.8 -5.1 – -6.9 -6.4 -7.3 -8.6 0.1 1.6 0.6 -2.9 -2.5 -3.4
Endoplasmic Reticulum 0 2.5 2.8 4.1 – 4.2 4.1 2.6 3.9 0 0 0 0 0 0
Endosome 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 2.7 – 4.5 4.1 7.6 6.1 0 0 0 0 0 0
Nucleus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 – 1.3 1.4 0 2.1 0 0 0 1.6 1.1 2.1

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 4.951 6.8147 6.2255 4.9251 4.9961 6.5595 6.3352 8.5145 7.1434 7.3146 6.5221 7.4828 5.8483 7.9144 6.749 6.9345 5.498 6.8393
Actin 0.0175 0.0007 0.0144 0.0128 0.0027 0.0036 0.0033 0.0004 0.0029 0.0025 0.0011 0.0008 0.0609 0.0004 0.0258 0.001 0.0127 0.0065
Bud 0.0006 0.0006 0.0051 0.0006 0.0024 0.0012 0.0004 0.0004 0.0007 0.0008 0.0006 0.0007 0.0008 0.0021 0.0017 0.0007 0.0004 0.0006
Bud Neck 0.0021 0.0046 0.0033 0.0007 0.0016 0.0012 0.0003 0.0002 0.0052 0.01 0.0119 0.0007 0.0033 0.0001 0.0014 0.0009 0.0008 0.0009
Bud Periphery 0.0005 0.0004 0.0013 0.0003 0.0031 0.0014 0.0003 0.0002 0.0004 0.0004 0.0003 0.0004 0.0007 0.0005 0.0023 0.0003 0.0003 0.0004
Bud Site 0.0041 0.0067 0.0117 0.0012 0.0028 0.0007 0.0008 0.001 0.0019 0.0016 0.0164 0.0001 0.0106 0.0013 0.0044 0.0013 0.0021 0.0002
Cell Periphery 0.0006 0.0006 0.0003 0.0003 0.0004 0.0008 0.0003 0.0002 0.0002 0.0003 0.001 0.0001 0.0005 0.0002 0.0003 0.0001 0.0001 0.0001
Cytoplasm 0.7167 0.9283 0.851 0.6962 0.7028 0.7929 0.7843 0.9384 0.8057 0.7935 0.7921 0.831 0.6838 0.9509 0.8248 0.8001 0.7397 0.7872
Cytoplasmic Foci 0.0392 0.0112 0.0221 0.0368 0.0275 0.0211 0.0255 0.0097 0.0207 0.0236 0.0265 0.022 0.0356 0.0113 0.0329 0.0602 0.0449 0.0233
Eisosomes 0.0004 0 0.0001 0.0001 0.0001 0 0 0 0.0001 0 0 0 0.0007 0 0.0001 0 0.0002 0
Endoplasmic Reticulum 0.0481 0.0078 0.0131 0.0314 0.0145 0.0408 0.0446 0.0234 0.019 0.0187 0.0131 0.0366 0.0585 0.0071 0.0198 0.0047 0.0075 0.0382
Endosome 0.0943 0.0251 0.0386 0.1446 0.0516 0.0886 0.0819 0.0151 0.0758 0.0989 0.0997 0.0697 0.0714 0.0158 0.0381 0.0742 0.068 0.0921
Golgi 0.0213 0.003 0.0077 0.0192 0.0131 0.0176 0.0121 0.0019 0.0139 0.0096 0.0109 0.011 0.028 0.0011 0.0129 0.0125 0.019 0.0094
Lipid Particles 0.0072 0.0002 0.0058 0.0196 0.0475 0.0001 0.0034 0.0001 0.0023 0.0015 0.0099 0.0039 0.0046 0.0001 0.0112 0.0083 0.0077 0.0043
Mitochondria 0.0113 0.0016 0.0042 0.0062 0.0193 0.0153 0.0058 0.0013 0.0258 0.0032 0.0018 0.0108 0.0065 0.0008 0.0037 0.003 0.0043 0.0069
None 0.0075 0.0008 0.003 0.002 0.0027 0.0008 0.0092 0.0011 0.0013 0.0015 0.001 0.0011 0.0048 0.0035 0.0033 0.0006 0.0159 0.0039
Nuclear Periphery 0.0054 0.0006 0.0016 0.0031 0.0019 0.0016 0.0054 0.0011 0.0025 0.0024 0.0023 0.001 0.0056 0.0006 0.0017 0.0009 0.037 0.0092
Nucleolus 0.0005 0.0003 0.0003 0.0001 0.0239 0 0.0001 0 0.0001 0.0002 0.0001 0 0.0001 0.0001 0.0015 0.0003 0.0006 0.0002
Nucleus 0.0029 0.0006 0.0013 0.0013 0.0248 0.0009 0.0022 0.0008 0.001 0.0023 0.0014 0.0008 0.0015 0.0006 0.0016 0.001 0.004 0.0021
Peroxisomes 0.0032 0.0014 0.0067 0.0088 0.0056 0.0001 0.0018 0 0.0038 0.0003 0.0012 0.002 0.0102 0.0001 0.004 0.0104 0.0175 0.0021
Punctate Nuclear 0.0045 0.0001 0.0011 0.0009 0.0325 0.0001 0.0012 0.0001 0.0002 0.0013 0.0002 0.0001 0.0018 0.0001 0.0011 0.0017 0.0066 0.0062
Vacuole 0.0091 0.0044 0.0058 0.0099 0.0144 0.0072 0.0139 0.0039 0.0129 0.0215 0.0063 0.0047 0.008 0.0025 0.0055 0.0138 0.007 0.0035
Vacuole Periphery 0.0032 0.0011 0.0014 0.0037 0.0046 0.0038 0.0028 0.0007 0.0038 0.006 0.0022 0.0024 0.002 0.0007 0.0017 0.004 0.0036 0.0026

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 17.4313 23.3738 24.6676 37.4809 32.0174 20.906 32.2988 34.383 34.6001 31.7562
Translational Efficiency 2.962 2.8886 2.5288 1.5318 1.9022 2.8067 1.9468 1.7105 2.0348 2.018

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘
Standard name
Human Ortholog
Description eIF4E-associated protein, competes with eIF4G for binding to eIF4E; accelerates mRNA degradation by promoting decapping, facilitated by interaction with eIF4E; essential for Puf5p mediated repression; associates with Puf5p and Dhh1p; inhibits cap-dependent translation; functions independently of eIF4E to maintain genetic stability; plays a role in cell growth, implicated in the TOR signaling cascade
Localization
Cell Percentages cytoplasm (98%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-5

Eap1

Eap1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Eap1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available