Standard name | |
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Human Ortholog | |
Description | 5-oxoprolinase; enzyme is ATP-dependent and functions as a dimer; similar to mouse Oplah gene; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; protein abundance increases in response to DNA replication stress |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 3 | 1 | 0 | 0 | 4 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 2 | 2 | 0 | 3 | 2 | 7 | 7 | 5 | 8 | 6 | 9 | 1 | 0 | 7 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 13 | 1 | 4 | 4 | 4 | 10 | 11 | 20 | 13 | 21 | 18 | 27 | 0 | 3 | 7 | 6 | 8 | 6 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 268 | 199 | 268 | 218 | 200 | 323 | 376 | 439 | 234 | 329 | 260 | 323 | 186 | 248 | 258 | 186 | 131 | 200 | 257 | 187 | 269 | 158 | 259 | 194 |
Endoplasmic Reticulum | 0 | 2 | 2 | 1 | 1 | 8 | 10 | 9 | 3 | 6 | 9 | 4 | 0 | 0 | 0 | 6 | 3 | 8 | 1 | 2 | 4 | 3 | 0 | 3 |
Endosome | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 1 | 2 | 3 | 0 | 1 | 1 | 1 |
Golgi | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 4 | 1 | 14 | 10 | 58 | 62 | 102 | 94 | 165 | 124 | 235 | 1 | 2 | 8 | 3 | 1 | 1 | 2 | 1 | 3 | 0 | 0 | 2 |
Nucleus | 1 | 1 | 1 | 1 | 1 | 3 | 5 | 9 | 1 | 2 | 1 | 6 | 0 | 3 | 2 | 6 | 4 | 5 | 0 | 2 | 0 | 0 | 1 | 0 |
Nuclear Periphery | 0 | 1 | 0 | 0 | 0 | 1 | 1 | 1 | 0 | 3 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 1 | 0 | 2 | 2 | 2 | 2 | 11 | 32 | 4 | 19 | 12 | 33 | 0 | 0 | 0 | 8 | 13 | 12 | 0 | 5 | 1 | 0 | 0 | 1 |
Unique Cell Count | 272 | 204 | 273 | 224 | 211 | 367 | 434 | 558 | 305 | 462 | 364 | 512 | 187 | 251 | 262 | 200 | 146 | 218 | 269 | 209 | 285 | 171 | 268 | 209 |
Labelled Cell Count | 284 | 211 | 281 | 240 | 221 | 408 | 485 | 624 | 355 | 553 | 431 | 642 | 188 | 257 | 284 | 219 | 162 | 234 | 269 | 209 | 285 | 171 | 268 | 209 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 11.9 | 8.9 | 6.7 | 6.5 | 7.0 | 6.7 | 6.4 | 6.5 | 6.1 | 5.8 | 5.8 | 5.5 | 9.3 | 9.6 | 8.3 | 9.8 | 10.8 | 11.0 | 9.3 | 10.0 | 10.6 |
Std Deviation (1e-4) | 1.7 | 1.6 | 0.9 | 1.0 | 1.3 | 1.3 | 1.2 | 1.3 | 1.3 | 1.2 | 1.1 | 1.1 | 1.4 | 1.7 | 1.4 | 2.4 | 3.0 | 2.8 | 1.4 | 2.0 | 1.9 |
Intensity Change (Log2) | – | – | – | -0.06 | 0.06 | -0.0 | -0.06 | -0.05 | -0.14 | -0.21 | -0.21 | -0.29 | 0.47 | 0.51 | 0.31 | 0.54 | 0.68 | 0.71 | 0.47 | 0.57 | 0.65 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 1.1 | 1.0 | 1.7 | 2.0 | 2.2 | 2.4 | 2.6 | 0 | 0 | 1.0 | 0 | 0 | 0 |
Cytoplasm | -0.6 | -2.1 | -4.8 | -5.2 | -7.4 | -7.6 | -9.0 | -8.9 | -10.9 | 1.2 | 0.6 | 0.3 | -2.8 | -3.9 | -3.3 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 3.8 | 0 | 6.7 | 6.3 | 7.4 | 9.9 | 11.1 | 10.6 | 13.3 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 1.7 | 3.4 | 0 | 2.7 | 2.2 | 3.7 | 0 | 0 | 0 | 0 | 4.3 | 3.2 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 4.1479 | 5.8953 | 5.7232 | 5.0409 | 4.4225 | 5.1601 | 4.7128 | 6.3515 | 5.1482 | 5.199 | 4.8324 | 5.1108 | 5.9482 | 6.5182 | 6.267 | 6.4588 | 6.3727 | 6.2594 |
Actin | 0.0002 | 0.0003 | 0.0007 | 0.0004 | 0.0003 | 0.0004 | 0.0577 | 0.001 | 0.0165 | 0.0143 | 0.042 | 0.0096 | 0.0198 | 0.0046 | 0.0249 | 0.0136 | 0.0311 | 0.0036 |
Bud | 0.0001 | 0.0004 | 0.0004 | 0.0002 | 0.0001 | 0.0002 | 0.0013 | 0.0007 | 0.0009 | 0.0008 | 0.0029 | 0.0002 | 0.004 | 0.0013 | 0.0087 | 0.0021 | 0.0023 | 0.0035 |
Bud Neck | 0.0002 | 0.0002 | 0.0004 | 0.0005 | 0.0004 | 0.0012 | 0.0123 | 0.0002 | 0.0003 | 0.0016 | 0.0014 | 0.0006 | 0.0052 | 0.0002 | 0.0007 | 0.0017 | 0.0295 | 0.001 |
Bud Periphery | 0.0001 | 0.0002 | 0.0003 | 0.0001 | 0.0001 | 0.0003 | 0.002 | 0.0004 | 0.0007 | 0.0009 | 0.0043 | 0.0002 | 0.0026 | 0.0006 | 0.0019 | 0.0008 | 0.0034 | 0.0013 |
Bud Site | 0.0002 | 0.0024 | 0.0014 | 0.0004 | 0.0002 | 0.0002 | 0.0046 | 0.0039 | 0.0026 | 0.0013 | 0.0125 | 0.0001 | 0.0094 | 0.0064 | 0.0111 | 0.0012 | 0.0192 | 0.0005 |
Cell Periphery | 0.0006 | 0.0008 | 0.0004 | 0.0002 | 0.0002 | 0.0002 | 0.0006 | 0.0002 | 0.0001 | 0.0001 | 0.0005 | 0 | 0.0005 | 0.0001 | 0.0003 | 0.0001 | 0.0002 | 0.0001 |
Cytoplasm | 0.9174 | 0.9683 | 0.9458 | 0.9371 | 0.8955 | 0.9515 | 0.6298 | 0.9043 | 0.8791 | 0.8172 | 0.723 | 0.8975 | 0.753 | 0.9182 | 0.8344 | 0.8367 | 0.7298 | 0.922 |
Cytoplasmic Foci | 0.0026 | 0.0036 | 0.004 | 0.0059 | 0.0055 | 0.0036 | 0.0421 | 0.0088 | 0.0205 | 0.0256 | 0.0312 | 0.0103 | 0.0274 | 0.0135 | 0.0109 | 0.0259 | 0.0256 | 0.0096 |
Eisosomes | 0 | 0 | 0 | 0 | 0.0006 | 0 | 0.0019 | 0 | 0.0001 | 0.0001 | 0.0002 | 0 | 0.0002 | 0 | 0.0003 | 0.0001 | 0.0001 | 0 |
Endoplasmic Reticulum | 0.019 | 0.0019 | 0.0035 | 0.0027 | 0.0017 | 0.0077 | 0.0092 | 0.0016 | 0.0024 | 0.004 | 0.0049 | 0.0029 | 0.0119 | 0.0016 | 0.0034 | 0.0021 | 0.0023 | 0.0011 |
Endosome | 0.0026 | 0.0027 | 0.0047 | 0.0087 | 0.0094 | 0.0061 | 0.0575 | 0.0032 | 0.0195 | 0.038 | 0.0674 | 0.015 | 0.02 | 0.0027 | 0.0112 | 0.0064 | 0.0351 | 0.0023 |
Golgi | 0.0002 | 0.0003 | 0.0004 | 0.0004 | 0.0004 | 0.0006 | 0.017 | 0.0012 | 0.0104 | 0.0211 | 0.0247 | 0.0041 | 0.006 | 0.0007 | 0.0025 | 0.004 | 0.0132 | 0.0004 |
Lipid Particles | 0.0004 | 0.0001 | 0.0015 | 0.0002 | 0 | 0.0001 | 0.0227 | 0.0002 | 0.0045 | 0.0161 | 0.0107 | 0.0016 | 0.0049 | 0.0003 | 0.0018 | 0.03 | 0.0011 | 0 |
Mitochondria | 0.0001 | 0.0002 | 0.0001 | 0.0002 | 0.0002 | 0.0024 | 0.0116 | 0.0003 | 0.002 | 0.0106 | 0.0263 | 0.0088 | 0.0026 | 0.0003 | 0.0116 | 0.0019 | 0.0139 | 0.0006 |
None | 0.0521 | 0.0142 | 0.0256 | 0.0363 | 0.0823 | 0.0202 | 0.0842 | 0.0681 | 0.0302 | 0.0213 | 0.0103 | 0.0393 | 0.1154 | 0.0448 | 0.0641 | 0.0527 | 0.0852 | 0.0468 |
Nuclear Periphery | 0.0012 | 0.0005 | 0.0007 | 0.0008 | 0.0003 | 0.0008 | 0.0073 | 0.0002 | 0.0006 | 0.0072 | 0.0052 | 0.0026 | 0.0017 | 0.0004 | 0.0011 | 0.0006 | 0.0008 | 0.0004 |
Nucleolus | 0 | 0 | 0.0001 | 0.0001 | 0.0002 | 0 | 0.0011 | 0 | 0.0001 | 0.0006 | 0.0016 | 0.0001 | 0.001 | 0 | 0.0002 | 0.0001 | 0.0001 | 0.0004 |
Nucleus | 0.0007 | 0.0014 | 0.0014 | 0.0015 | 0.0008 | 0.0013 | 0.0028 | 0.0007 | 0.0009 | 0.0029 | 0.0029 | 0.0011 | 0.0025 | 0.0008 | 0.0045 | 0.0013 | 0.0009 | 0.0045 |
Peroxisomes | 0 | 0.0001 | 0.0001 | 0 | 0 | 0 | 0.0234 | 0.0025 | 0.0057 | 0.0064 | 0.0043 | 0.0033 | 0.007 | 0.0022 | 0.0028 | 0.0152 | 0.003 | 0.0005 |
Punctate Nuclear | 0.0001 | 0.0001 | 0.0002 | 0.0004 | 0.0002 | 0.0001 | 0.0054 | 0.0002 | 0.0007 | 0.0058 | 0.0099 | 0.0017 | 0.0018 | 0.0003 | 0.0015 | 0.002 | 0.0007 | 0.0008 |
Vacuole | 0.002 | 0.0022 | 0.0078 | 0.0035 | 0.0012 | 0.0025 | 0.0041 | 0.0021 | 0.0018 | 0.0027 | 0.0106 | 0.0008 | 0.0026 | 0.0007 | 0.0015 | 0.0012 | 0.0019 | 0.0005 |
Vacuole Periphery | 0.0001 | 0.0002 | 0.0004 | 0.0003 | 0.0002 | 0.0005 | 0.0014 | 0.0002 | 0.0003 | 0.0013 | 0.0033 | 0.0004 | 0.0005 | 0.0001 | 0.0004 | 0.0003 | 0.0007 | 0.0002 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 21.7194 | 18.1385 | 17.251 | 20.3474 | 21.3054 | 20.2793 | 20.3417 | 24.4076 | 25.6176 | 24.1274 |
Translational Efficiency | 1.1368 | 1.0809 | 0.9258 | 0.9791 | 0.6984 | 1.0835 | 1.0278 | 0.678 | 0.6754 | 0.6871 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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