ORF
Human Ortholog
Description Protein of unknown function; may interact with ribosomes, based on co-purification experiments; similar to transcriptional regulators from the zinc cluster (binuclear cluster) family; null mutant is sensitive to caffeine
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0.05 0 0.05 0.05 0.05 – 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.95 0.97 0.98 0.98 0.98 0.96 0.94 – 0.88 0.86 0.79 0.82 0.99 0.99 0.99 0.98 0.99 0.97 0.74 0.83 0.73 0.73 0.64 0.68
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0.09 0.06 0.09 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.05 0 0 0 0 0 0.06 – 0.2 0.19 0.28 0.15 0 0 0 0 0 0 0.12 0.08 0.13 0.14 0.19 0.13
Nucleus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 1 1 0 0 0 – 0 1 0 0 0 0 0 0 0 1 2 2 9 6 7 10
Bud 0 1 0 1 0 3 2 – 13 11 13 9 1 0 1 0 0 2 1 0 3 3 12 15
Bud Neck 1 0 27 19 27 27 35 – 20 13 7 29 0 0 0 1 4 4 2 0 1 3 6 6
Bud Site 0 0 0 0 0 0 1 – 1 1 1 5 0 0 0 0 0 0 – – – – – –
Cell Periphery 2 0 5 9 1 0 5 – 4 6 2 4 0 0 3 11 4 5 1 1 12 5 6 6
Cytoplasm 263 193 569 518 547 549 625 – 580 532 411 481 414 506 576 570 310 346 220 160 355 199 307 320
Endoplasmic Reticulum 1 1 4 1 0 0 1 – 0 2 0 6 0 3 1 50 18 32 3 0 0 1 5 6
Endosome 1 0 0 0 1 1 0 – 0 0 0 0 0 0 0 4 0 3 5 3 3 0 5 4
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 1 1 1 0 0 1 2 1 1
Mitochondria 14 8 0 3 1 21 39 – 130 119 148 86 4 4 3 20 2 5 35 14 60 38 88 61
Nucleus 1 0 1 1 4 5 3 – 6 8 5 3 2 0 1 0 1 2 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 1 0 0 0 1 0 0 0 0 1 0 0 0 1 0
Nucleolus 0 0 0 0 1 0 0 – 0 0 2 0 3 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 1 – 0 0 0 1 1 0 1 2 0 0 1 0 0 1 0 1
SpindlePole 0 0 1 2 3 3 14 – 11 13 8 27 0 1 0 1 1 3 2 1 5 0 8 11
Vac/Vac Membrane 1 1 3 1 1 1 2 – 1 3 4 2 0 0 1 4 2 6 10 3 5 7 17 17
Unique Cell Count 276 199 579 527 559 571 666 662 619 520 587 419 512 580 583 314 358 296 194 486 273 478 472
Labelled Cell Count 284 204 611 556 586 610 728 766 710 601 653 425 515 587 664 343 410 296 194 486 273 478 472


Ambiguous

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.6 3.6 4.5 4.0 4.6 3.6 3.5 – 3.1 3.2 3.1 3.5 3.6 3.7 3.8 5.3 5.4 5.5 3.4 3.6 3.9
Std Deviation (1e-4) 0.6 0.8 0.8 1.0 1.6 1.3 1.0 – 1.0 1.2 1.1 0.7 1.1 1.0 1.3 1.3 1.1 1.5 1.0 1.5 1.5
Intensity Change (Log2) – – – -0.15 0.05 -0.32 -0.35 – -0.53 -0.47 -0.51 -0.34 -0.29 -0.29 -0.24 0.25 0.28 0.3 -0.42 -0.32 -0.19


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -0.1991 -0.7246 -0.8363 -1.2093 -0.8238 -0.849 -0.6218 -0.4431 -0.5608 -0.3848 -1.1574 -0.7269 -1.0025 -1.0078 -0.6485 -0.5797 -0.6725 -1.1054
Actin 0.0322 0.0006 0.0068 0.0002 0.0432 0.0122 0.0453 0.0028 0.0099 0.0207 0.0306 0.0007 0.0319 0.0006 0.0191 0.0018 0.014 0.015
Bud 0.001 0.0013 0.0012 0.0004 0.0006 0.0002 0.0006 0.0037 0.0018 0.0013 0.0001 0.0003 0.0006 0.0025 0.0009 0.0002 0.0006 0.001
Bud Neck 0.0013 0 0.0013 0 0.0004 0.0006 0.0046 0.0002 0.001 0.0004 0.0003 0.0002 0.0025 0.0001 0.0002 0.0001 0.0013 0.002
Bud Periphery 0.0021 0.0025 0.0009 0.0007 0.0011 0.0006 0.001 0.0044 0.0021 0.0017 0.0001 0.0006 0.0012 0.0037 0.0021 0.0004 0.001 0.004
Bud Site 0.0026 0.0004 0.0109 0.0003 0.0012 0 0.0023 0.0043 0.0211 0.0041 0.0002 0 0.0122 0.0036 0.0033 0.0009 0.0126 0.0002
Cell Periphery 0.0005 0.0001 0.0002 0.0001 0.0001 0 0.0003 0.0003 0.0004 0.0003 0.0002 0.0001 0.0008 0.0002 0.0002 0.0001 0.0002 0.0001
Cytoplasm 0.0147 0.011 0.0087 0.0106 0.0068 0.0259 0.0155 0.0267 0.0227 0.0195 0.037 0.052 0.015 0.0199 0.0301 0.0224 0.0159 0.0555
Cytoplasmic Foci 0.0176 0.0019 0.007 0.0034 0.002 0.0182 0.0102 0.0034 0.0096 0.0056 0.0028 0.0061 0.0248 0.0046 0.0134 0.0029 0.0163 0.0078
Eisosomes 0.0008 0.0002 0.0007 0.0001 0.0003 0.0003 0.0007 0.0003 0.0003 0.0003 0.0011 0.0001 0.0007 0.0003 0.0003 0.0001 0.0002 0.0002
Endoplasmic Reticulum 0.0148 0.0006 0.0007 0.0004 0.0015 0.0009 0.0026 0.0005 0.0006 0.0012 0.0008 0.0005 0.0009 0.0006 0.0016 0.0013 0.0013 0.0006
Endosome 0.0368 0.0006 0.0017 0.0007 0.0055 0.0018 0.0096 0.0004 0.0022 0.0038 0.0017 0.0024 0.0129 0.0029 0.0124 0.0019 0.0383 0.0008
Golgi 0.0082 0.0001 0.0007 0.0001 0.0073 0.0003 0.0049 0.0001 0.0015 0.0015 0.0014 0.0003 0.0056 0.0007 0.0106 0.0009 0.0336 0.0006
Lipid Particles 0.0152 0.0004 0.0048 0.0004 0.0026 0.0015 0.0023 0.0002 0.004 0.0021 0.0011 0.001 0.0128 0.0018 0.0057 0.0011 0.037 0.0038
Mitochondria 0.0183 0.0005 0.0007 0.0003 0.0033 0.0002 0.0095 0.0004 0.0012 0.0011 0.0006 0.0003 0.0072 0.0007 0.0089 0.036 0.0152 0.0012
None 0.7595 0.9782 0.9414 0.9801 0.9208 0.9281 0.8756 0.9447 0.9098 0.9277 0.9188 0.9313 0.8311 0.9483 0.8696 0.8865 0.7366 0.9003
Nuclear Periphery 0.0346 0.0003 0.0009 0.0004 0.0012 0.0022 0.0044 0.0006 0.0008 0.0028 0.0012 0.0019 0.001 0.0006 0.0016 0.0005 0.0083 0.0002
Nucleolus 0.0022 0.0002 0.0013 0.0002 0.0001 0.0002 0.0005 0.0006 0.0008 0.0005 0.0001 0.0001 0.0009 0.0019 0.0003 0.0017 0.0016 0.0001
Nucleus 0.0114 0.0003 0.0015 0.0002 0.0002 0.0006 0.0059 0.0027 0.0018 0.0009 0.0003 0.0005 0.0006 0.0025 0.0008 0.0106 0.0024 0.0004
Peroxisomes 0.0066 0.0001 0.0028 0.0002 0.0005 0.0009 0.0016 0.0002 0.0031 0.0012 0.0006 0.0002 0.0277 0.0007 0.0159 0.0019 0.021 0.0055
Punctate Nuclear 0.0099 0.0003 0.0053 0.0007 0.0005 0.005 0.0016 0.0033 0.0045 0.0023 0.0009 0.0011 0.0045 0.0017 0.0011 0.0282 0.0091 0.0004
Vacuole 0.0059 0.0003 0.0005 0.0004 0.0004 0.0002 0.0007 0.0004 0.0005 0.0008 0.0002 0.0002 0.0046 0.0018 0.0013 0.0003 0.0307 0.0002
Vacuole Periphery 0.0039 0.0001 0.0001 0.0001 0.0003 0.0001 0.0005 0.0001 0.0002 0.0004 0.0001 0.0001 0.0005 0.0003 0.0006 0.0002 0.0026 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 6.2516 2.7943 3.4558 6.7398 4.0527 3.1241 5.6506 6.9919 6.1381 7.0905
Translational Efficiency – – – – – – – – – –

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency –
ORF
Human Ortholog
Description Protein of unknown function; may interact with ribosomes, based on co-purification experiments; similar to transcriptional regulators from the zinc cluster (binuclear cluster) family; null mutant is sensitive to caffeine
Localization
Cell Percentages cytoplasm (16%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

YKL222C

YKL222C


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ykl222c-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available