Standard name
Human Ortholog
Description Sterol binding protein involved in the export of acetylated sterols; secreted glycoprotein and member of the CAP protein superfamily (cysteine-rich secretory proteins (CRISP), antigen 5, and pathogenesis related 1 proteins); sterol export function is redundant with that of PRY1; may be involved in detoxification of hydrophobic compounds; PRY2 has a paralog, PRY1, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.7 0.62 – 0.88 0.71 0.75 0.71 0.61 0.61 0.67 0.74 0.67 0.89 0.96 0.99 0.31 0.41 0.34 0.2 0.13 0.29 0.23 0.27 0.31
Endoplasmic Reticulum 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.05 0 0 0 0 0 0
Endosome 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0 0 0.11 0 0 0 0
Golgi 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.2 0 – 0 0 0 0.07 0 0.1 0.06 0.17 0.09 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 – 0 0.05 0.09 0.09 0.13 0.08 0.08 0.06 0.06 0 0 0 0.2 0.16 0.11 0 0 0 0 0 0
Nuclear Periphery 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.23 0.41 – 0.16 0.37 0.38 0.3 0.35 0.42 0.33 0.26 0.33 0.12 0 0 0.61 0.52 0.69 0.67 0.64 0.56 0.6 0.59 0.55
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 – 0 0 0 0 0 0 1 0 1 1 0 0 0 0 0 0 0 0 0 2 2
Bud 1 0 – 0 0 0 1 3 6 6 10 7 0 0 0 0 0 0 0 4 1 8 10 8
Bud Neck 0 0 – 0 0 0 1 0 0 0 0 0 1 0 0 0 0 0 0 0 0 1 1 1
Bud Site 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 – 1 0 0 0 1 0 1 0 0 1 0 1 0 0 0 0 0 0 0 1 0
Cytoplasm 128 69 – 154 215 213 295 249 228 270 215 259 391 551 584 67 53 66 41 16 19 45 135 142
Endoplasmic Reticulum 1 2 – 0 0 0 3 0 0 1 0 0 2 1 1 9 7 10 1 0 0 1 2 4
Endosome 1 0 – 2 8 0 0 0 0 2 0 2 9 0 0 9 9 4 8 13 2 8 12 9
Golgi 0 0 – 0 0 0 0 0 0 0 0 0 1 1 0 2 1 4 0 2 0 0 1 1
Mitochondria 36 5 – 3 0 6 30 17 38 25 49 35 1 0 1 4 4 6 5 4 2 5 11 15
Nucleus 2 2 – 0 15 25 37 51 30 31 16 24 0 1 1 44 20 21 1 0 0 0 2 0
Nuclear Periphery 1 0 – 0 0 1 4 5 3 3 5 1 0 1 0 0 0 0 0 0 0 0 1 2
Nucleolus 0 2 – 1 0 0 0 0 0 0 0 0 0 0 0 2 1 0 0 0 0 0 1 0
Peroxisomes 0 0 – 0 0 0 0 0 0 0 0 0 1 1 1 0 0 1 0 0 0 0 0 1
SpindlePole 0 0 – 4 2 0 1 1 1 1 1 2 3 1 0 1 1 1 4 1 0 3 8 6
Vac/Vac Membrane 43 46 – 28 111 107 126 141 157 133 76 129 51 20 9 133 66 136 138 82 36 117 300 253
Unique Cell Count 183 112 176 304 285 418 405 371 404 290 389 437 573 590 218 128 197 207 129 66 196 506 459
Labelled Cell Count 213 126 193 351 352 498 468 463 474 372 460 462 577 598 271 162 249 207 129 66 196 506 459


Vacuole

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.5 4.7 – 4.5 5.7 4.7 4.5 5.2 4.7 4.8 4.3 4.9 4.9 4.9 4.7 7.1 7.2 7.3 4.8 4.8 5.1
Std Deviation (1e-4) 0.7 1.1 – 1.4 1.7 1.3 1.4 1.8 1.8 1.5 1.5 1.6 1.0 1.0 1.4 2.8 2.5 2.9 1.3 1.3 1.6
Intensity Change (Log2) – – – – – – – – – – – – – – – – – – – – –


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -0.702 0.1857 -0.2052 -0.3568 -0.2022 -0.1887 -0.9312 -0.0185 -0.1771 -0.1786 -0.2702 -0.1234 -1.5431 -0.5908 -1.0169 -0.3912 -0.815 -1.1521
Actin 0.0114 0.0002 0.003 0.0005 0.019 0.0004 0.005 0.0004 0.0002 0.0001 0.0208 0.0034 0.0469 0.0001 0.0047 0.009 0.0018 0.0012
Bud 0.0017 0.0062 0.0028 0.0008 0.002 0.0037 0.0026 0.005 0.0024 0.001 0.0017 0.0006 0.0013 0.0006 0.0016 0.0011 0.0025 0.001
Bud Neck 0.0026 0.0033 0.0116 0.0014 0.0039 0.0048 0.0022 0.0086 0.004 0.0069 0.0019 0.005 0.0044 0.001 0.0013 0.0019 0.0037 0.0012
Bud Periphery 0.0062 0.0053 0.0044 0.0017 0.0056 0.0057 0.004 0.0079 0.0023 0.0019 0.0022 0.0014 0.0019 0.0009 0.0047 0.0017 0.0032 0.0026
Bud Site 0.0019 0.0235 0.0187 0.0011 0.0052 0.0013 0.0169 0.009 0.0036 0.0039 0.0019 0.0011 0.0164 0.0028 0.0034 0.004 0.0107 0.0005
Cell Periphery 0.0012 0.0019 0.0014 0.0009 0.0012 0.0011 0.001 0.0017 0.0011 0.0013 0.0006 0.0008 0.0009 0.0015 0.0015 0.0008 0.0006 0.001
Cytoplasm 0.093 0.0797 0.0591 0.0398 0.0443 0.1042 0.0809 0.107 0.0787 0.0832 0.0669 0.1215 0.0861 0.0834 0.0572 0.025 0.1354 0.1057
Cytoplasmic Foci 0.0683 0.0669 0.071 0.0775 0.0685 0.0784 0.0776 0.0562 0.0682 0.0464 0.0638 0.0478 0.0485 0.0489 0.043 0.0588 0.0798 0.0378
Eisosomes 0.0004 0.0001 0.0002 0.0004 0.0002 0.0001 0.0005 0.0001 0.0001 0.0002 0.0002 0.0001 0.0007 0 0.0001 0.0001 0 0
Endoplasmic Reticulum 0.0088 0.0061 0.0044 0.0023 0.0037 0.0041 0.0078 0.007 0.0025 0.0022 0.0031 0.0037 0.0074 0.003 0.0057 0.0015 0.0066 0.0029
Endosome 0.1822 0.1534 0.1592 0.2433 0.2196 0.182 0.1344 0.1233 0.2151 0.1982 0.1992 0.1612 0.1609 0.1938 0.2206 0.1895 0.1972 0.1754
Golgi 0.0069 0.0029 0.0049 0.0066 0.011 0.0027 0.0088 0.0022 0.0062 0.003 0.0158 0.0027 0.0107 0.0043 0.0052 0.0095 0.013 0.0039
Lipid Particles 0.0152 0.0146 0.0241 0.0338 0.0433 0.0218 0.0283 0.0072 0.0159 0.0157 0.0285 0.0213 0.0236 0.0148 0.0115 0.0149 0.0085 0.0081
Mitochondria 0.0197 0.0218 0.0128 0.0269 0.0215 0.0083 0.0205 0.0074 0.0189 0.007 0.0076 0.0042 0.0183 0.0148 0.036 0.0108 0.0438 0.0069
None 0.3032 0.209 0.2915 0.1921 0.1278 0.2089 0.4402 0.3302 0.2045 0.1438 0.2699 0.1918 0.2396 0.1826 0.1165 0.1562 0.1251 0.1374
Nuclear Periphery 0.0129 0.0117 0.0056 0.004 0.0327 0.0089 0.0069 0.0098 0.0037 0.0034 0.0036 0.0139 0.0135 0.0032 0.0094 0.004 0.0167 0.0038
Nucleolus 0.0044 0.0071 0.0115 0.0139 0.0098 0.0064 0.0027 0.0042 0.0066 0.018 0.0052 0.0027 0.0038 0.004 0.0027 0.0021 0.0041 0.0014
Nucleus 0.0125 0.0226 0.0135 0.0299 0.0136 0.0204 0.0053 0.0133 0.0212 0.0129 0.012 0.0112 0.0046 0.0052 0.0055 0.0034 0.0386 0.0057
Peroxisomes 0.0079 0.0043 0.0052 0.0077 0.0164 0.004 0.0298 0.0036 0.0089 0.0034 0.0149 0.0105 0.0244 0.0014 0.0042 0.0053 0.0058 0.0012
Punctate Nuclear 0.0094 0.0035 0.0075 0.0076 0.005 0.0094 0.0092 0.0018 0.004 0.0026 0.0033 0.0044 0.0073 0.001 0.0019 0.0324 0.005 0.0009
Vacuole 0.2001 0.3268 0.2637 0.2716 0.3071 0.2913 0.1008 0.2751 0.2966 0.3989 0.2585 0.358 0.234 0.3975 0.3992 0.3994 0.2645 0.4533
Vacuole Periphery 0.0303 0.0293 0.0239 0.0361 0.0388 0.0319 0.0146 0.0191 0.0353 0.0459 0.0184 0.0328 0.0449 0.0352 0.064 0.0688 0.0332 0.0483

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 363.9006 110.5937 39.9237 60.5801 416.9006 256.2046 87.6436 48.9546 50.398 332.1837
Translational Efficiency 1.8819 1.6332 0.9631 1.1217 1.6133 2.6042 1.6486 1.5725 1.2992 1.7766

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✔
Standard name
Human Ortholog
Description Sterol binding protein involved in the export of acetylated sterols; secreted glycoprotein and member of the CAP protein superfamily (cysteine-rich secretory proteins (CRISP), antigen 5, and pathogenesis related 1 proteins); sterol export function is redundant with that of PRY1; may be involved in detoxification of hydrophobic compounds; PRY2 has a paralog, PRY1, that arose from the whole genome duplication
Localization
Cell Percentages vacuole (61%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Pry2

Pry2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Pry2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available