Standard name | |
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Human Ortholog | |
Description | Component of the GARP (Golgi-associated retrograde protein) complex; GARP is required for the recycling of proteins from endosomes to the late Golgi, and for mitosis after DNA damage induced checkpoint arrest; links the (VFT/GARP) complex to the SNARE Tlg1p; members of the GARP complex are Vps51p-Vps52p-Vps53p-Vps54p |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 1 | 5 | 4 | 0 | 4 | 2 | – | 2 | 0 | 2 | 2 | 2 | 6 | 1 | 0 | 0 | 2 | 1 | 31 | 21 | 4 | 2 | 3 | 5 |
Bud | 17 | 6 | 41 | 16 | 19 | 29 | – | 52 | 46 | 52 | 29 | 32 | 4 | 2 | 3 | 3 | 11 | 1 | 3 | 2 | 0 | 0 | 0 | 0 |
Bud Neck | 3 | 0 | 7 | 0 | 1 | 0 | – | 3 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 3 | 1 | 0 | 0 | 0 | 0 |
Bud Site | 8 | 0 | 4 | 1 | 7 | 5 | – | 16 | 11 | 16 | 12 | 12 | 0 | 0 | 0 | 1 | 1 | 2 | – | – | – | – | – | – |
Cell Periphery | 3 | 3 | 0 | 3 | 1 | 1 | – | 0 | 3 | 3 | 1 | 3 | 1 | 0 | 0 | 0 | 2 | 4 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 30 | 22 | 29 | 18 | 29 | 13 | – | 35 | 14 | 21 | 14 | 11 | 6 | 6 | 10 | 48 | 52 | 59 | 1 | 2 | 1 | 0 | 0 | 0 |
Endoplasmic Reticulum | 1 | 12 | 3 | 2 | 5 | 4 | – | 2 | 3 | 3 | 3 | 6 | 8 | 3 | 1 | 6 | 4 | 6 | 0 | 1 | 3 | 0 | 0 | 0 |
Endosome | 213 | 92 | 2 | 3 | 45 | 5 | – | 18 | 9 | 10 | 5 | 8 | 36 | 23 | 66 | 147 | 106 | 101 | 267 | 182 | 142 | 34 | 41 | 44 |
Golgi | 0 | 49 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 1 | 12 | 8 | 6 | 24 | 12 | 11 | 107 | 157 | 24 | 25 | 32 | 44 |
Mitochondria | 449 | 304 | 314 | 247 | 249 | 509 | – | 694 | 499 | 542 | 414 | 410 | 48 | 55 | 45 | 36 | 35 | 25 | 133 | 80 | 75 | 11 | 17 | 19 |
Nucleus | 2 | 0 | 0 | 2 | 2 | 2 | – | 6 | 2 | 7 | 2 | 1 | 0 | 2 | 1 | 8 | 8 | 7 | 2 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 1 | 3 | – | 5 | 5 | 2 | 10 | 7 | 0 | 1 | 1 | 0 | 1 | 1 | 1 | 1 | 2 | 0 | 0 | 0 |
Nucleolus | 13 | 3 | 0 | 4 | 6 | 16 | – | 28 | 17 | 8 | 15 | 10 | 0 | 0 | 1 | 4 | 8 | 7 | 3 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 166 | 67 | 13 | 41 | 80 | 23 | – | 65 | 17 | 61 | 30 | 33 | 19 | 7 | 31 | 80 | 100 | 89 | 155 | 97 | 125 | 43 | 40 | 37 |
SpindlePole | 81 | 22 | 50 | 19 | 40 | 33 | – | 94 | 32 | 51 | 36 | 24 | 9 | 4 | 19 | 161 | 117 | 120 | 23 | 12 | 15 | 1 | 2 | 4 |
Vac/Vac Membrane | 21 | 16 | 3 | 0 | 3 | 6 | – | 7 | 6 | 6 | 3 | 7 | 3 | 2 | 4 | 19 | 13 | 13 | 14 | 12 | 6 | 0 | 2 | 0 |
Unique Cell Count | 668 | 468 | 370 | 276 | 367 | 556 | 811 | 542 | 624 | 454 | 458 | 109 | 94 | 146 | 353 | 314 | 302 | 756 | 579 | 414 | 123 | 142 | 160 | |
Labelled Cell Count | 1008 | 601 | 470 | 356 | 492 | 651 | 1027 | 664 | 785 | 576 | 567 | 152 | 114 | 188 | 538 | 472 | 447 | 756 | 579 | 414 | 123 | 142 | 160 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 4.9 | 5.9 | 3.7 | 3.8 | 4.6 | 3.8 | – | 4.0 | 3.8 | 4.2 | 4.0 | 4.2 | 5.8 | 5.8 | 5.7 | 7.9 | 7.6 | 7.7 | 5.4 | 5.9 | 6.0 |
Std Deviation (1e-4) | 0.7 | 0.9 | 0.7 | 1.1 | 1.3 | 0.9 | – | 1.0 | 1.1 | 1.1 | 1.0 | 1.2 | 1.1 | 1.2 | 1.0 | 2.1 | 2.2 | 2.4 | 1.0 | 1.2 | 1.1 |
Intensity Change (Log2) | – | – | – | 0.03 | 0.32 | 0.03 | – | 0.11 | 0.02 | 0.18 | 0.1 | 0.17 | 0.63 | 0.63 | 0.62 | 1.08 | 1.02 | 1.04 | 0.54 | 0.67 | 0.68 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | -2.3 | -2.7 | -3.3 | -5.8 | -3.7 | -5.7 |
Bud Neck | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | -0.6 | 0 | -3.9 | – | -2.5 | -3.7 | -3.1 | -3.1 | -3.6 | -0.8 | -0.5 | -0.4 | 2.5 | 3.5 | 4.5 |
Endoplasmic Reticulum | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 11.0 | 9.2 | 13.5 | 13.7 | 11.9 | 11.8 |
Golgi | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 5.1 | 3.8 | 0 |
Mitochondria | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | -8.7 | -5.7 | -12.0 | -20.1 | -19.2 | -19.8 |
Nucleus | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 5.1 | 0 | 6.5 | 7.7 | 9.9 | 9.3 |
SpindlePole | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | -1.5 | -2.5 | -0.2 | 9.5 | 7.2 | 7.8 |
Vacuole | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.6 | 2.9 | 3.0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 1.0626 | 1.1981 | 1.2756 | 1.0832 | 1.2855 | 1.0349 | 0.6488 | 0.6733 | 0.8177 | 0.6603 | 0.8862 | 0.5485 | 2.0619 | 2.01 | 1.6753 | 1.3673 | 2.7996 | 2.2155 |
Actin | 0.0153 | 0.0041 | 0.0172 | 0.0178 | 0.0209 | 0.0065 | 0.0285 | 0.0071 | 0.0449 | 0.036 | 0.0131 | 0.0105 | 0.0763 | 0.0139 | 0.0294 | 0.0094 | 0.0854 | 0.0028 |
Bud | 0.0004 | 0.0071 | 0.0006 | 0.0043 | 0.0008 | 0.0005 | 0.0006 | 0.0049 | 0.0007 | 0.0015 | 0.0004 | 0.0089 | 0.0022 | 0.0029 | 0.0018 | 0.0001 | 0.0006 | 0.0001 |
Bud Neck | 0.0013 | 0.0006 | 0.0021 | 0.0032 | 0.0017 | 0.0019 | 0.0027 | 0.0006 | 0.0014 | 0.0002 | 0.0008 | 0.001 | 0.0049 | 0.0037 | 0.0005 | 0.0004 | 0.0024 | 0.0023 |
Bud Periphery | 0.0005 | 0.0026 | 0.0006 | 0.0046 | 0.001 | 0.0004 | 0.0006 | 0.0023 | 0.001 | 0.001 | 0.0005 | 0.0086 | 0.0029 | 0.0007 | 0.0011 | 0.0001 | 0.0007 | 0.0001 |
Bud Site | 0.0019 | 0.0111 | 0.0026 | 0.0025 | 0.0044 | 0.0005 | 0.0063 | 0.0058 | 0.006 | 0.0007 | 0.0009 | 0.0006 | 0.0165 | 0.0126 | 0.0026 | 0.0003 | 0.0028 | 0.0002 |
Cell Periphery | 0.0001 | 0.0002 | 0.0001 | 0.0003 | 0.0001 | 0.0001 | 0.0002 | 0.0002 | 0.0003 | 0.0002 | 0.0001 | 0.0004 | 0.0007 | 0.0003 | 0.0002 | 0 | 0.0004 | 0 |
Cytoplasm | 0.0047 | 0.0112 | 0.0081 | 0.0055 | 0.0198 | 0.0109 | 0.0264 | 0.0196 | 0.0062 | 0.0112 | 0.0026 | 0.0262 | 0.0148 | 0.0159 | 0.0153 | 0.0075 | 0.0215 | 0.0141 |
Cytoplasmic Foci | 0.1914 | 0.2674 | 0.2073 | 0.172 | 0.265 | 0.2105 | 0.2246 | 0.2693 | 0.2296 | 0.2656 | 0.2167 | 0.2512 | 0.2043 | 0.2489 | 0.2311 | 0.2064 | 0.1915 | 0.2492 |
Eisosomes | 0.0002 | 0 | 0.0001 | 0.0003 | 0.0002 | 0 | 0.0003 | 0.0001 | 0.0003 | 0.0002 | 0.0004 | 0.0001 | 0.0009 | 0.0002 | 0.0001 | 0 | 0.0003 | 0 |
Endoplasmic Reticulum | 0.0014 | 0.0007 | 0.0011 | 0.0004 | 0.0006 | 0.0006 | 0.0042 | 0.0015 | 0.0009 | 0.0008 | 0.0005 | 0.001 | 0.0061 | 0.0011 | 0.0017 | 0.001 | 0.0107 | 0.0004 |
Endosome | 0.2486 | 0.2742 | 0.2635 | 0.2487 | 0.1788 | 0.1887 | 0.2094 | 0.1884 | 0.1883 | 0.195 | 0.1359 | 0.138 | 0.1587 | 0.1905 | 0.1841 | 0.2815 | 0.3213 | 0.1906 |
Golgi | 0.135 | 0.1436 | 0.1621 | 0.1558 | 0.1148 | 0.1345 | 0.1024 | 0.119 | 0.1699 | 0.0824 | 0.1197 | 0.1025 | 0.1032 | 0.1448 | 0.1441 | 0.1413 | 0.1327 | 0.1848 |
Lipid Particles | 0.1226 | 0.0649 | 0.0867 | 0.0713 | 0.1147 | 0.1013 | 0.1561 | 0.0703 | 0.0638 | 0.0817 | 0.0493 | 0.1069 | 0.1318 | 0.0931 | 0.0801 | 0.0778 | 0.0531 | 0.1088 |
Mitochondria | 0.0244 | 0.0167 | 0.0237 | 0.0469 | 0.0467 | 0.0173 | 0.0172 | 0.0277 | 0.0376 | 0.0095 | 0.0571 | 0.0053 | 0.0299 | 0.0219 | 0.0273 | 0.0114 | 0.0082 | 0.0059 |
None | 0.0036 | 0.0039 | 0.0111 | 0.034 | 0.0127 | 0.0035 | 0.062 | 0.0224 | 0.0347 | 0.1258 | 0.0426 | 0.0262 | 0.0357 | 0.0091 | 0.022 | 0.0017 | 0.0067 | 0.027 |
Nuclear Periphery | 0.0072 | 0.0003 | 0.0015 | 0.0002 | 0.0017 | 0.0007 | 0.0047 | 0.0013 | 0.0005 | 0.0009 | 0.0008 | 0.0004 | 0.0222 | 0.0004 | 0.0034 | 0.0002 | 0.0358 | 0.0002 |
Nucleolus | 0.0005 | 0.0004 | 0.0003 | 0.0005 | 0.0009 | 0.0002 | 0.0006 | 0.0009 | 0.0004 | 0.0005 | 0.0021 | 0.0003 | 0.0018 | 0.0007 | 0.0008 | 0.0001 | 0.0007 | 0.0001 |
Nucleus | 0.0037 | 0.0006 | 0.0005 | 0.0003 | 0.0018 | 0.0003 | 0.0016 | 0.0008 | 0.0003 | 0.001 | 0.0008 | 0.0003 | 0.0074 | 0.0006 | 0.0033 | 0.0001 | 0.0152 | 0.0001 |
Peroxisomes | 0.2292 | 0.1785 | 0.2044 | 0.2254 | 0.2033 | 0.3165 | 0.1422 | 0.2474 | 0.2082 | 0.1784 | 0.3489 | 0.3074 | 0.1507 | 0.2335 | 0.2456 | 0.2569 | 0.0969 | 0.2103 |
Punctate Nuclear | 0.0032 | 0.0069 | 0.0028 | 0.0022 | 0.0063 | 0.0016 | 0.0044 | 0.0024 | 0.0015 | 0.0037 | 0.0042 | 0.0022 | 0.021 | 0.0021 | 0.0032 | 0.0007 | 0.0074 | 0.001 |
Vacuole | 0.0029 | 0.0037 | 0.0022 | 0.0022 | 0.0025 | 0.0022 | 0.0032 | 0.0067 | 0.0023 | 0.0029 | 0.0009 | 0.0014 | 0.0045 | 0.0022 | 0.0016 | 0.0021 | 0.0033 | 0.0012 |
Vacuole Periphery | 0.0018 | 0.0011 | 0.0014 | 0.0015 | 0.0014 | 0.0011 | 0.0018 | 0.0015 | 0.0012 | 0.001 | 0.0014 | 0.0006 | 0.0033 | 0.0009 | 0.0008 | 0.001 | 0.0024 | 0.0008 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 5.5727 | 11.9561 | 4.436 | 8.311 | 7.2253 | 6.6836 | 11.5851 | 14.2784 | 11.0024 | 10.1919 |
Translational Efficiency | 6.3729 | 1.7972 | 5.2528 | 3.3649 | 4.3741 | 4.7211 | 2.3154 | 2.7826 | 3.3377 | 3.3017 |
Dataset | Hit |
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Protein Concentration | ✔ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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