Standard name
Human Ortholog
Description Alpha subunit of translation initiation factor eIF2B; guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; positive regulator of GCN4 expression; assembles into filaments with Gcd2p, Gcd6p, Gcd7p, and Sui2p as cells approach stationary phase and under cytosolic acidification and starvation conditions; human homolog EIF2B1 can complement yeast null mutant
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 – 0.05 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0.05 0.06 0.07 0 0 0 0 0.06 – 0.11 0 0 0 0 0 0
Cytoplasm 0.75 0.9 0.87 0.91 0.82 0.76 0.59 0.62 0.64 0.68 0.6 0.58 0.93 0.92 0.95 0.88 – 0.89 0.79 0.89 0.81 0.68 0.7 0.72
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0.07 0.05 0.06 – 0.05 0.05 0 0.06 0.09 0.09 0.13
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0
Mitochondria 0.26 0.17 0.16 0.36 0.37 0.28 0.51 0.49 0.45 0.43 0.52 0.46 0 0 0 0 – 0.11 0 0 0.06 0 0.05 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0
Vac/Vac Membrane 0.06 0 0 0 0 0 0.06 0.06 0.06 0 0 0 0 0 0 0 – 0.05 0 0 0 0.1 0.11 0.06
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 4 0 0 0 0 0 0 0 0 1 0 4 1 1 1 1 – 1 1 0 0 0 0 0
Bud 0 0 0 0 0 3 1 3 4 0 1 3 0 0 0 0 – 0 0 0 1 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 1 0 0 0 0 1
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 – – – – – –
Cell Periphery 2 0 2 0 0 5 1 3 8 13 10 8 2 3 8 1 – 2 1 0 3 0 0 0
Cytoplasm 72 36 103 20 40 91 91 95 103 155 85 110 70 118 175 14 – 17 87 73 104 73 112 161
Endoplasmic Reticulum 2 1 5 0 2 1 2 1 7 3 2 9 3 9 9 1 – 1 5 1 7 9 13 28
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 – 0 0 0 0 0 1 1
Golgi 1 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 – 0 0 0 0 1 2 3
Mitochondria 25 7 19 8 18 33 79 74 73 99 74 88 1 0 0 0 – 2 3 1 7 3 8 7
Nucleus 0 1 0 0 1 1 0 0 0 1 0 3 0 0 0 0 – 0 1 0 0 1 0 0
Nuclear Periphery 0 0 1 0 0 0 4 0 0 0 3 1 0 0 0 0 – 0 0 0 1 0 0 0
Nucleolus 1 0 0 0 0 0 2 0 0 1 0 1 0 0 0 0 – 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0
Vac/Vac Membrane 6 0 0 0 0 1 9 9 10 3 5 8 0 1 1 0 – 1 2 3 3 11 17 13
Unique Cell Count 96 40 119 22 49 119 154 152 162 229 142 190 75 128 184 16 19 110 83 130 108 161 225
Labelled Cell Count 113 45 130 28 61 135 189 185 206 276 180 235 77 132 195 17 24 110 83 130 108 161 225


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 9.5 8.3 8.0 8.0 6.5 6.5 6.0 5.8 5.7 5.4 5.7 5.7 10.6 10.6 11.1 13.1 – 14.5 11.1 10.9 11.8
Std Deviation (1e-4) 2.1 1.4 1.6 1.4 1.0 1.8 1.7 1.4 1.2 1.2 1.2 1.1 2.1 1.8 1.5 2.5 – 3.8 1.7 2.2 1.9
Intensity Change (Log2) – – – -0.01 -0.31 -0.3 -0.43 -0.46 -0.5 -0.56 -0.5 -0.49 0.4 0.4 0.46 0.7 – 0.85 0.46 0.44 0.55


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0
Cell Periphery 0 0 0 0 0 0 1.7 2.1 0 0 0 0 0 – 0
Cytoplasm 0.6 -0.8 -2.0 -5.0 -4.4 -4.3 -3.8 -4.8 -5.3 1.5 1.4 2.6 0.1 – 0.4
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0.2 0 1.0 0.3 0 – 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0
Mitochondria 0 3.0 2.2 6.0 5.6 5.1 5.1 6.1 5.5 -3.3 -4.7 -5.6 0 – 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 5.1579 9.0268 7.8945 7.0597 6.3599 7.6808 7.7058 10.6795 9.4957 9.63 8.7836 9.5733 6.0081 9.2923 7.1088 7.0755 6.9099 7.4027
Actin 0.0112 0.0007 0.0073 0.0002 0.0004 0.0017 0.0159 0.0047 0.0155 0.0006 0.0016 0.0057 0.0075 0.0012 0.0026 0.0047 0.0155 0.0014
Bud 0.0015 0.0007 0.0015 0.0007 0.0011 0.0006 0.0009 0.0014 0.0015 0.0022 0.0015 0.0011 0.0006 0.0017 0.0009 0.0008 0.0008 0.0006
Bud Neck 0.0015 0.0005 0.0007 0.0043 0.0007 0.0017 0.0006 0.0005 0.0009 0.0006 0.0007 0.0017 0.0013 0.0004 0.0014 0.0005 0.0008 0.0011
Bud Periphery 0.0019 0.0003 0.0011 0.0003 0.0007 0.0006 0.0013 0.0011 0.0013 0.0022 0.003 0.0013 0.0007 0.001 0.001 0.0007 0.0006 0.0007
Bud Site 0.0062 0.0108 0.0028 0.0006 0.0017 0.0004 0.0024 0.0071 0.0039 0.0005 0.0006 0.0004 0.0019 0.003 0.002 0.0004 0.0015 0.0002
Cell Periphery 0.0015 0.0004 0.0006 0.0002 0.0002 0.001 0.0024 0.002 0.0006 0.0003 0.0012 0.0007 0.0013 0.0007 0.001 0.0003 0.0005 0.0003
Cytoplasm 0.5786 0.8755 0.7746 0.5765 0.57 0.6581 0.6935 0.848 0.7135 0.5063 0.4897 0.6923 0.6592 0.8322 0.6784 0.5016 0.6345 0.6998
Cytoplasmic Foci 0.0363 0.0156 0.0285 0.0482 0.0484 0.03 0.0192 0.0186 0.0361 0.0269 0.027 0.0312 0.039 0.0237 0.0396 0.0235 0.0255 0.0239
Eisosomes 0.0006 0 0 0 0 0 0.0006 0.0002 0.0001 0 0.0001 0.0001 0.0001 0 0.0001 0.0005 0.0002 0
Endoplasmic Reticulum 0.0904 0.0213 0.0188 0.0107 0.0179 0.0851 0.059 0.0205 0.0255 0.0077 0.0982 0.0658 0.0678 0.0261 0.0255 0.0663 0.086 0.0956
Endosome 0.1123 0.0437 0.0902 0.2286 0.2488 0.1435 0.0768 0.0474 0.1254 0.2543 0.2406 0.1077 0.0926 0.0537 0.1517 0.293 0.1605 0.1097
Golgi 0.01 0.0047 0.0066 0.0085 0.0148 0.0233 0.0158 0.017 0.0134 0.0307 0.016 0.0388 0.01 0.0104 0.0136 0.0143 0.0175 0.0201
Lipid Particles 0.0156 0.0002 0.0021 0.0022 0.0016 0.0005 0.0092 0.0012 0.0023 0.0001 0.0009 0.0009 0.0045 0.0002 0.0056 0.0024 0.0046 0.0005
Mitochondria 0.0086 0.0022 0.0089 0.0017 0.0055 0.03 0.0164 0.0136 0.0031 0.074 0.0192 0.035 0.0036 0.0107 0.0086 0.0071 0.0074 0.0276
None 0.0421 0.0012 0.0062 0.0028 0.0077 0.0026 0.0412 0.0013 0.0072 0.0006 0.0328 0.0055 0.0388 0.0099 0.0135 0.0072 0.0102 0.0015
Nuclear Periphery 0.0134 0.0013 0.0086 0.0033 0.0046 0.0021 0.0133 0.0009 0.0035 0.002 0.0071 0.0014 0.0146 0.0013 0.0071 0.0046 0.0073 0.004
Nucleolus 0.001 0 0.0002 0.0001 0.0002 0 0.0012 0 0.0001 0 0.0001 0 0.0006 0.0002 0.0006 0.0001 0.0003 0
Nucleus 0.0154 0.0012 0.0044 0.0022 0.0026 0.0011 0.004 0.0008 0.0019 0.001 0.0017 0.0007 0.0057 0.0011 0.0072 0.0013 0.0027 0.0012
Peroxisomes 0.0018 0.0001 0.0004 0.0009 0.0004 0.0002 0.0012 0.0001 0.0008 0.0002 0.0002 0.0003 0.0017 0.0001 0.003 0.0004 0.0023 0.0002
Punctate Nuclear 0.0048 0.0002 0.001 0.0009 0.0034 0.0001 0.0048 0.0001 0.0009 0.0001 0.0005 0.0001 0.0196 0.0002 0.0058 0.0003 0.0043 0.0008
Vacuole 0.0398 0.0166 0.0284 0.0895 0.0564 0.0118 0.0164 0.0116 0.0333 0.0533 0.0316 0.0061 0.0227 0.0188 0.024 0.0538 0.0127 0.0069
Vacuole Periphery 0.0056 0.0026 0.0071 0.0176 0.0128 0.0056 0.0037 0.0018 0.0092 0.0364 0.0256 0.0033 0.0061 0.0032 0.0067 0.0161 0.0041 0.0039

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 48.0786 46.2028 34.2845 49.2416 51.4272 24.026 39.9255 35.5628 40.3804 49.141
Translational Efficiency 1.5788 1.1358 1.6585 0.9456 1.0995 2.795 1.4836 1.3805 1.217 1.2102

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✔
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Alpha subunit of translation initiation factor eIF2B; guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; positive regulator of GCN4 expression; assembles into filaments with Gcd2p, Gcd6p, Gcd7p, and Sui2p as cells approach stationary phase and under cytosolic acidification and starvation conditions; human homolog EIF2B1 can complement yeast null mutant
Localization
Cell Percentages cytoplasm (98%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-5

Gcn3

Gcn3


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Gcn3-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available