Standard name
Human Ortholog
Description Member of the ChAPs (Chs5p-Arf1p-binding proteins) family; part of the exomer complex with Chs5p to mediate export of specific cargo proteins, including Chs3p, from the Golgi to the plasma membrane; BCH2 has a paralog, CHS6, that arose from the whole genome duplication

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0.13 0 0.05 0 0 0 0 0 0 0 0 0 0.08 0 0 0 0.06 0 0.05 0.07 0.11
Bud 0.06 0.05 0.09 0.08 0.11 0.07 0.08 0.14 0.08 0.06 0.11 0.08 0.06 0 0.05 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0.05 0.05 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0.07 0.06 0.08 0 0.07 0 0 0 0 0 0.06 0 0.15 0.1 0.19 0 0 0 0 0 0
Endoplasmic Reticulum 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0.06 0 0 0 0 0 0
Endosome 0.11 0.1 0.16 0.13 0.14 0.08 0.06 0 0 0 0 0.06 0.22 0.24 0.25 0.37 0.31 0.28 0.23 0.31 0.21 0.2 0.21
Golgi 0.09 0.07 0.05 0 0 0 0 0 0 0 0 0 0.06 0 0.08 0.08 0 0.38 0.31 0.15 0.27 0.27 0.27
Mitochondria 0.83 0.83 0.41 0.62 0.45 0.73 0.69 0.88 0.87 0.9 0.81 0.79 0.55 0.61 0.16 0.17 0.15 0.09 0.2 0.22 0.21 0.18 0.11
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0.07 0.11 0.15 0.1 0.12 0.08 0.05 0.05 0 0.06 0.11 0.2 0.22 0 0 0 0.18 0.11 0.22 0.19 0.23 0.23
SpindlePole 0 0 0.27 0.15 0.27 0.18 0.2 0.1 0.14 0.12 0.22 0 0.07 0.05 0.32 0.34 0.3 0 0 0 0 0 0
Vac/Vac Membrane 0 0.07 0.05 0 0 0 0 0 0 0 0 0 0 0 0.06 0.09 0.07 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 7 10 29 4 14 3 13 6 5 0 7 8 1 2 17 5 2 9 22 11 13 20 33
Bud 12 15 20 15 27 24 32 60 37 17 29 23 16 13 11 5 7 1 4 0 0 1 3
Bud Neck 0 0 5 0 1 1 3 1 1 3 0 0 0 1 2 1 0 0 0 0 0 0 1
Bud Site 0 0 0 1 1 3 4 15 16 15 13 1 0 0 2 1 0
Cell Periphery 0 2 1 1 0 1 2 0 0 0 0 2 2 1 1 1 1 0 0 0 0 0 0
Cytoplasm 3 7 15 11 21 14 26 8 13 12 8 13 16 14 32 16 32 0 0 0 0 1 0
Endoplasmic Reticulum 7 15 6 2 5 4 6 4 2 2 2 9 5 3 10 6 10 1 0 1 4 3 4
Endosome 22 31 34 25 37 26 23 7 1 0 7 19 62 83 51 57 52 63 85 98 53 62 64
Golgi 19 21 11 8 2 0 0 0 0 0 1 11 18 8 16 13 5 86 115 49 68 85 85
Mitochondria 172 267 89 120 115 245 263 375 392 259 216 234 157 208 33 26 25 21 74 69 53 56 35
Nucleus 1 1 2 2 3 4 4 2 1 4 6 0 0 1 6 1 1 0 1 0 1 0 0
Nuclear Periphery 1 2 0 1 1 2 2 2 5 0 0 2 2 4 3 0 1 0 0 0 1 1 0
Nucleolus 1 1 0 0 1 3 11 9 17 8 4 4 4 8 0 2 1 0 0 1 0 0 0
Peroxisomes 8 22 24 29 25 40 29 21 22 8 15 32 57 74 6 7 7 41 39 71 49 70 71
SpindlePole 2 8 59 29 69 61 76 43 64 35 58 6 20 16 67 53 50 1 7 7 5 3 6
Vac/Vac Membrane 8 21 11 2 6 6 6 5 5 1 5 5 5 7 13 14 12 1 12 4 2 2 3
Unique Cell Count 206 321 219 193 256 335 382 425 452 289 266 295 288 343 208 156 166 230 369 322 258 313 313
Labelled Cell Count 263 423 306 250 328 437 500 558 581 364 371 369 365 443 270 208 206 230 369 322 258 313 313


Late Golgi

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.0 6.1 6.0 5.5 5.9 4.7 4.9 4.2 4.0 3.7 4.4 5.3 5.6 5.6 6.7 7.2 6.3 5.2 5.3 5.8
Std Deviation (1e-4) 0.7 0.9 1.1 1.3 1.3 1.0 1.4 0.7 0.8 0.7 0.9 0.8 1.1 1.2 1.2 1.6 1.2 1.1 1.0 1.5
Intensity Change (Log2) -0.12 -0.04 -0.36 -0.3 -0.54 -0.61 -0.72 -0.45 -0.18 -0.1 -0.12 0.15 0.26 0.06 -0.23 -0.19 -0.07


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches -4.2 -2.9 0 -4.6 0 0 0 0 -4.6 -6.1 -6.4 -1.7 -3.3 -4.3
Bud -0.5 0.5 0 -0.3 0 0 0 0 -0.5 -1.6 -2.6 -1.5 -2.3 -1.9
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -0.5 0.6 -1.4 0 -3.2 -2.4 -1.3 -2.0 -1.2 -0.6 -1.4 2.8 1.2 3.7
Endoplasmic Reticulum 0 -0.6 0 0 0 0 0 0 0.2 0 0 1.1 0 1.6
Endosome -0.7 -0.3 0 -3.8 0 0 0 0 -3.3 1.7 2.5 2.3 4.7 3.7
Golgi -0.4 -2.8 0 0 0 0 0 0 -0.7 0.6 -1.7 1.1 1.3 -1.0
Mitochondria 4.4 0.9 0 6.8 0 0 0 0 9.0 3.1 4.6 -5.7 -5.0 -5.4
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 1.2 -0.4 0 -1.4 0 0 0 0 0 2.7 3.2 -3.3 -2.2 -2.4
SpindlePole -2.9 0 0 -2.0 0 0 0 0 -8.4 -6.1 -7.6 1.2 1.5 0.7
Vacuole -2.3 -1.6 -2.2 -2.5 -3.0 -3.1 -3.4 -1.9 -2.1 -2.1 -2.0 0.6 1.5 0.9

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.0743 0.5272 0.8401 0.3711 0.7544 0.7919 1.1756 1.7658 1.7003 1.7919 1.5725 1.5351 0.5909 1.1357 0.837 1.5795 0.0573 0.7366
Actin 0.0753 0.0112 0.0318 0.0173 0.0243 0.0165 0.043 0.0253 0.0225 0.0337 0.0703 0.0216 0.0252 0.0237 0.0566 0.0717 0.0065 0.0222
Bud 0.0011 0.0019 0.0007 0.0004 0.0005 0.0003 0.0003 0.0008 0.0062 0.0004 0 0.0003 0.0007 0.0029 0.0006 0.0075 0.0002 0.0001
Bud Neck 0.0078 0.0008 0.0027 0.0019 0.0004 0.0015 0.0008 0.0006 0.0012 0.001 0.0002 0.0011 0.0014 0.0007 0.001 0.0025 0.0014 0.0017
Bud Periphery 0.0014 0.0014 0.0009 0.0005 0.0008 0.0003 0.0003 0.0006 0.0051 0.0005 0 0.0003 0.0012 0.003 0.0006 0.0148 0.0002 0.0002
Bud Site 0.0201 0.0133 0.0022 0.0029 0.0006 0.0007 0.0011 0.0084 0.0096 0.0009 0.0001 0.0009 0.0034 0.0048 0.0014 0.0417 0.0005 0.0004
Cell Periphery 0.0005 0.0002 0.0003 0.0002 0.0001 0.0001 0.0001 0.0001 0.0002 0.0001 0 0.0001 0.0005 0.0002 0.0001 0.0046 0.0001 0.0001
Cytoplasm 0.0091 0.0181 0.015 0.002 0.0019 0.0021 0.0033 0.0094 0.0175 0.0019 0.0007 0.0041 0.0018 0.0161 0.0048 0.0032 0.0012 0.0016
Cytoplasmic Foci 0.1247 0.2222 0.1842 0.1397 0.1651 0.1652 0.1695 0.2076 0.2012 0.1471 0.1602 0.139 0.1971 0.2004 0.1268 0.1446 0.1429 0.1393
Eisosomes 0.0007 0.0001 0.0004 0.0004 0.0002 0.0001 0.0001 0.0001 0.0001 0.0001 0 0.0001 0.0002 0.0001 0.0001 0.001 0.0001 0.0001
Endoplasmic Reticulum 0.0054 0.0009 0.0014 0.0019 0.003 0.0007 0.0013 0.0008 0.0003 0.0003 0.0003 0.0004 0.001 0.0022 0.0005 0.0022 0.0005 0.0004
Endosome 0.1968 0.2315 0.2641 0.3348 0.2194 0.1984 0.2218 0.2257 0.1778 0.1908 0.1841 0.1477 0.1954 0.2333 0.1264 0.1438 0.2237 0.1765
Golgi 0.1737 0.2269 0.2316 0.2134 0.2413 0.3341 0.2128 0.2643 0.2628 0.2481 0.2817 0.289 0.1711 0.2565 0.3016 0.2275 0.32 0.3446
Lipid Particles 0.1409 0.0783 0.0693 0.0998 0.1035 0.0497 0.0651 0.0181 0.0288 0.0208 0.111 0.0847 0.153 0.0643 0.0784 0.0762 0.1772 0.092
Mitochondria 0.0486 0.037 0.0361 0.0552 0.0927 0.0428 0.0201 0.0223 0.0361 0.0179 0.0029 0.0569 0.0188 0.0305 0.0444 0.1145 0.0222 0.0326
None 0.0051 0.0202 0.0051 0.0023 0.0012 0.0006 0.0007 0.0068 0.0007 0.0002 0.0001 0.0002 0.0004 0.013 0.0073 0.0017 0.0002 0.0002
Nuclear Periphery 0.0248 0.0006 0.0009 0.0025 0.0194 0.0038 0.0024 0.0002 0.0002 0.0002 0 0.0001 0.0004 0.0017 0.0016 0.0028 0.0002 0.0001
Nucleolus 0.0015 0.0003 0.0003 0.0006 0.0002 0.0002 0.0001 0.0001 0.0003 0.0001 0 0.0001 0.0003 0.0006 0.0002 0.0016 0.0001 0.0001
Nucleus 0.0227 0.0004 0.0003 0.0007 0.0015 0.0005 0.0003 0.0002 0.0003 0.0001 0 0.0001 0.0004 0.0006 0.0006 0.0025 0.0001 0
Peroxisomes 0.1221 0.1271 0.1466 0.1122 0.1168 0.1754 0.2514 0.205 0.2216 0.3336 0.1867 0.2499 0.2161 0.1407 0.2401 0.1137 0.0984 0.185
Punctate Nuclear 0.0092 0.0014 0.0012 0.0029 0.0015 0.0042 0.001 0.001 0.0043 0.0005 0.0002 0.0005 0.0011 0.001 0.0052 0.0016 0.0004 0.0005
Vacuole 0.0048 0.0046 0.0029 0.0045 0.0018 0.0013 0.003 0.0014 0.0022 0.0011 0.0006 0.0017 0.0085 0.0024 0.0008 0.0158 0.0022 0.0011
Vacuole Periphery 0.0037 0.0015 0.0022 0.0038 0.0038 0.0014 0.0019 0.001 0.0012 0.0008 0.0009 0.0013 0.0021 0.0014 0.0008 0.0046 0.0017 0.0014

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 13.4444 10.7308 12.7404 13.5669 6.848 9.118 13.7794 15.0853 11.544 14.0657
Translational Efficiency 0.4679 0.5026 0.4325 0.4958 0.7767 0.6458 0.5867 0.4545 0.561 0.5013

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency
Standard name
Human Ortholog
Description Member of the ChAPs (Chs5p-Arf1p-binding proteins) family; part of the exomer complex with Chs5p to mediate export of specific cargo proteins, including Chs3p, from the Golgi to the plasma membrane; BCH2 has a paralog, CHS6, that arose from the whole genome duplication
Localization
Cell Percentages
Cell Cycle Regulation No
Subcompartmental Group N/A

Bch2

Bch2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Bch2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available