Standard name
Human Ortholog
Description Class E protein of the vacuolar protein-sorting (Vps) pathway; binds Vps4p and directs it to dissociate ESCRT-III complexes; forms a functional and physical complex with Ist1p; human ortholog may be altered in breast tumors
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0.05 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0.11 0.05 0.14 0.13 0.15 0.19 – 0.1 0.14 0.13 0.09 0.17 0.19 0.23 0.14 0.13 0.17 0 0.05 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0.05
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.1 0.17 0.05 0.16 0.05 0.45 0.17 – 0.47 0.44 0.5 0.45 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.9 0.73 0.92 0.72 0.71 0.62 0.64 – 0.66 0.65 0.65 0.72 0.79 0.78 0.72 0.76 0.74 0.73 0.85 0.65 0.73 0.64 0.58 0.51
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0.1 0 0.07 0.1 0.05 0.07 – 0 0 0 0 0 0 0.05 0.11 0.11 0.09 0.07 0.13 0.13 0.2 0.22 0.25
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 1 0 0 0 0 0 – 0 0 0 1 0 0 0 0 0 0 0 2 0 0 0 0
Bud 0 2 0 0 0 0 0 – 2 2 4 3 0 0 0 2 0 2 2 3 1 0 8 4
Bud Neck 0 0 1 0 1 0 0 – 0 0 3 0 0 0 0 0 0 0 0 1 0 1 3 2
Bud Site 0 0 0 0 0 6 0 – 6 7 12 9 0 0 0 1 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 1 0 2 1 1 0 0 0 0 0 0
Cytoplasm 14 39 5 15 17 23 45 – 27 33 32 19 33 45 75 71 60 72 3 20 2 2 3 8
Endoplasmic Reticulum 1 6 1 0 0 0 1 – 0 0 0 0 0 1 1 22 18 12 0 0 0 0 2 2
Endosome 3 4 1 1 1 0 5 – 1 1 2 0 3 2 3 10 12 6 8 22 2 3 8 14
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 1 2 0 0 5 1 0 1 0 0 0 2
Mitochondria 40 62 5 17 6 68 40 – 123 101 126 97 1 1 0 10 15 16 3 9 1 2 6 8
Nucleus 360 267 100 76 90 93 155 – 174 150 164 154 155 187 230 385 347 313 355 261 96 84 182 139
Nuclear Periphery 0 0 0 1 0 0 0 – 1 4 0 1 0 0 2 1 2 3 0 0 1 1 0 4
Nucleolus 2 0 0 1 1 2 2 – 2 1 1 2 0 1 0 0 0 0 3 11 1 4 12 7
Peroxisomes 1 0 0 0 0 0 0 – 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 3 2 0 1 0 6 – 1 5 1 2 1 1 0 1 0 5 7 10 1 2 13 6
Vac/Vac Membrane 11 36 4 7 12 8 18 – 11 7 8 8 7 6 15 54 50 37 30 53 17 26 70 67
Unique Cell Count 398 368 109 105 126 151 242 263 231 253 215 197 241 320 508 470 429 421 405 132 132 315 272
Labelled Cell Count 432 420 119 118 129 200 272 348 311 353 297 201 247 326 559 510 468 421 405 132 132 315 272


Endosome

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.7 4.6 5.0 5.2 5.8 3.9 5.0 – 4.2 4.7 4.4 4.4 6.1 6.3 6.2 6.6 7.4 6.5 5.3 5.3 5.9
Std Deviation (1e-4) 0.7 0.9 0.7 1.7 1.5 1.1 2.0 – 1.1 1.8 1.4 1.3 1.6 2.2 1.8 1.4 1.5 1.5 2.0 1.6 2.0
Intensity Change (Log2) – – – 0.05 0.22 -0.37 0.0 – -0.24 -0.1 -0.18 -0.18 0.28 0.33 0.31 0.4 0.56 0.38 0.08 0.1 0.25


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – 0 0 0
Bud Neck – – – – – – – – – – – – 0 0 0
Bud Site – – – – – – – – – – – – 0 0 0
Cell Periphery – – – – – – – – – – – – 0 0 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – 0 0 0
Endosome – – – – – – – – – – – – 0 0 0
Golgi – – – – – – – – – – – – 0 0 0
Mitochondria – – – – – – – – – – – – 0 0 0
Nuclear Periphery – – – – – – – – – – – – 0 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – 0 0 0
Peroxisomes – – – – – – – – – – – – 0 0 0
SpindlePole – – – – – – – – – – – – 0 0 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – 0 0 0
Cytoplasm – – – – – – – – – – – – 0 0 0
Nucleus – – – – – – – – – – – – 0 0 0
Vacuole – – – – – – – – – – – – 2.3 2.3 1.7

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.1218 0.0713 -0.0513 -0.2572 -0.4083 0.0144 2.2305 1.663 1.562 1.3974 1.6426 1.5115 4.0016 4.5565 4.5363 3.9909 3.6335 4.296
Actin 0.0264 0.0001 0.0076 0.0004 0.0006 0.0011 0.0126 0.0001 0.0215 0.0126 0.0057 0 0.0088 0 0.0031 0 0 0
Bud 0.0001 0 0.0001 0 0 0.0001 0.0004 0 0.0001 0.0005 0.0014 0 0.0002 0 0.0001 0 0 0
Bud Neck 0.0004 0.0002 0.0001 0 0.0002 0.0012 0.0106 0.0004 0.0022 0.0006 0.0007 0.0015 0.001 0.0003 0.0055 0.0001 0.0002 0.0013
Bud Periphery 0.0001 0 0.0002 0 0 0.0001 0.0014 0 0.0002 0.0005 0.0041 0.0001 0.0005 0.0001 0.0001 0 0 0
Bud Site 0.0002 0.0002 0.0001 0 0.0001 0.0001 0.0044 0.0002 0.0023 0.0043 0.001 0.0001 0.0005 0.0003 0.0003 0 0.0001 0.0001
Cell Periphery 0 0 0 0 0 0 0.0004 0 0.0001 0.0001 0.0005 0 0.0001 0 0 0 0 0
Cytoplasm 0.0217 0.0215 0.0054 0.0012 0.0059 0.0019 0.0126 0.0136 0.0042 0.0012 0.0058 0.002 0.0078 0.0082 0.0053 0.0009 0.0046 0.003
Cytoplasmic Foci 0.003 0.0006 0.0014 0 0.0064 0.0002 0.0128 0.0001 0.008 0.0101 0.0005 0.0001 0.003 0.0001 0.0007 0 0 0.0002
Eisosomes 0.0001 0 0.0004 0 0 0 0.0001 0 0.0002 0.0001 0.0001 0 0.0002 0 0 0 0 0
Endoplasmic Reticulum 0.0056 0.0009 0.001 0.0005 0.0001 0.0004 0.0033 0.0004 0.0011 0.0003 0.0018 0.0001 0.002 0.0004 0.0002 0 0 0.0001
Endosome 0.0036 0.0014 0.0019 0.0002 0.0034 0.0022 0.0174 0.0003 0.0041 0.0031 0.007 0.0001 0.0083 0.0004 0.0004 0 0 0.0005
Golgi 0.0011 0 0.0024 0 0.0026 0.0014 0.0029 0 0.0024 0.0024 0.0033 0 0.0012 0 0.0007 0 0 0
Lipid Particles 0.0017 0.0001 0.0082 0 0.0359 0.0036 0.0059 0 0.004 0.0058 0.0004 0 0.0019 0 0.0013 0 0 0.0001
Mitochondria 0.0005 0.0003 0.0019 0.0007 0.0006 0.002 0.0042 0.0002 0.0015 0.001 0.0311 0.0002 0.0023 0.0003 0.0003 0.0001 0.0001 0.0001
None 0.0045 0.0098 0.0033 0.0002 0.0108 0.0013 0.0062 0.0117 0.0114 0.0008 0.0285 0.0131 0.0213 0.0208 0.0155 0.0002 0.0435 0.0024
Nuclear Periphery 0.0159 0.0219 0.0237 0.0475 0.0037 0.014 0.0532 0.0053 0.0203 0.0043 0.005 0.0014 0.0123 0.0064 0.0028 0.0029 0.0033 0.0026
Nucleolus 0.0039 0.0082 0.0044 0.0017 0.0083 0.0143 0.0185 0.0063 0.0054 0.0076 0.0189 0.0119 0.0103 0.0081 0.0043 0.0043 0.0189 0.0105
Nucleus 0.9006 0.9193 0.9303 0.9457 0.8519 0.9436 0.7952 0.9533 0.8982 0.9332 0.8444 0.9624 0.9038 0.9475 0.9511 0.9895 0.9261 0.9664
Peroxisomes 0.0043 0 0.0021 0 0.0269 0.0002 0.0117 0 0.0062 0.0059 0.0001 0 0.0015 0 0.0033 0 0 0
Punctate Nuclear 0.0047 0.0139 0.0049 0.0006 0.0425 0.0112 0.0202 0.0065 0.0053 0.0052 0.0357 0.0066 0.0116 0.006 0.0047 0.0019 0.003 0.0122
Vacuole 0.0011 0.0012 0.0002 0.0002 0.0001 0.0004 0.0035 0.0013 0.0007 0.0002 0.0028 0.0003 0.001 0.0007 0.0002 0 0.0001 0.0002
Vacuole Periphery 0.0004 0.0003 0.0003 0.001 0.0001 0.0006 0.0023 0.0002 0.0007 0.0001 0.0014 0.0001 0.0006 0.0002 0 0 0 0

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 30.5006 20.8502 13.0915 33.2855 12.7941 30.4837 28.7846 34.4772 37.7076 34.6252
Translational Efficiency 2.6759 3.4957 5.4139 2.3636 5.6369 3.0221 2.6149 2.5569 2.5886 2.2948

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Class E protein of the vacuolar protein-sorting (Vps) pathway; binds Vps4p and directs it to dissociate ESCRT-III complexes; forms a functional and physical complex with Ist1p; human ortholog may be altered in breast tumors
Localization
Cell Percentages nucleus (93%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-3

Did2

Did2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Did2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available