Standard name | |
---|---|
Human Ortholog | |
Description | Putative redox protein containing a thioredoxin fold; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 1 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 7 | 1 | 1 | 4 | 2 | 2 | 3 | 5 | 5 |
Bud | 0 | 4 | 6 | 8 | 4 | 9 | – | 14 | 14 | 13 | 11 | 13 | 1 | 5 | 1 | 3 | 1 | 1 | 1 | 0 | 1 | 0 | 3 | 5 |
Bud Neck | 0 | 2 | 2 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 2 | 1 | 0 | 4 | 3 |
Bud Site | 0 | 0 | 1 | 1 | 2 | 1 | – | 5 | 8 | 4 | 2 | 9 | 0 | 0 | 0 | 1 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 1 | 1 | 2 | 2 | 3 | – | 0 | 3 | 5 | 2 | 2 | 1 | 2 | 0 | 12 | 2 | 5 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 91 | 55 | 54 | 56 | 74 | 31 | – | 41 | 14 | 26 | 18 | 13 | 117 | 158 | 191 | 13 | 7 | 3 | 48 | 22 | 12 | 23 | 44 | 46 |
Endoplasmic Reticulum | 4 | 4 | 0 | 0 | 0 | 0 | – | 0 | 2 | 1 | 0 | 2 | 2 | 0 | 0 | 6 | 0 | 1 | 3 | 0 | 1 | 0 | 1 | 2 |
Endosome | 1 | 5 | 1 | 2 | 43 | 6 | – | 4 | 0 | 1 | 1 | 0 | 5 | 9 | 9 | 21 | 2 | 8 | 7 | 6 | 9 | 5 | 10 | 5 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 2 | 20 | 4 | 2 | 1 | 1 | 0 | 3 | 13 | 4 |
Mitochondria | 51 | 55 | 57 | 114 | 107 | 188 | – | 254 | 191 | 242 | 122 | 161 | 17 | 32 | 30 | 14 | 1 | 12 | 86 | 69 | 75 | 33 | 56 | 61 |
Nucleus | 1 | 1 | 0 | 1 | 1 | 2 | – | 4 | 4 | 8 | 3 | 2 | 0 | 1 | 1 | 3 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 1 |
Nuclear Periphery | 1 | 1 | 1 | 1 | 1 | 0 | – | 7 | 3 | 2 | 5 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 |
Nucleolus | 0 | 0 | 1 | 1 | 0 | 0 | – | 2 | 4 | 4 | 2 | 2 | 1 | 0 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 |
Peroxisomes | 0 | 0 | 0 | 2 | 16 | 4 | – | 6 | 2 | 5 | 0 | 0 | 4 | 6 | 7 | 2 | 0 | 4 | 0 | 0 | 0 | 1 | 1 | 2 |
SpindlePole | 2 | 0 | 4 | 5 | 8 | 5 | – | 20 | 6 | 9 | 4 | 10 | 0 | 0 | 1 | 6 | 1 | 5 | 3 | 2 | 1 | 2 | 2 | 1 |
Vac/Vac Membrane | 9 | 7 | 2 | 6 | 20 | 17 | – | 8 | 10 | 13 | 2 | 1 | 10 | 12 | 10 | 24 | 4 | 13 | 14 | 11 | 3 | 5 | 12 | 14 |
Unique Cell Count | 138 | 112 | 107 | 154 | 221 | 219 | 299 | 205 | 271 | 138 | 175 | 146 | 201 | 234 | 106 | 20 | 42 | 178 | 129 | 119 | 84 | 164 | 168 | |
Labelled Cell Count | 160 | 136 | 130 | 199 | 278 | 266 | 365 | 261 | 333 | 172 | 218 | 158 | 227 | 254 | 133 | 25 | 56 | 178 | 129 | 119 | 84 | 164 | 168 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 3.6 | 4.1 | 3.8 | 3.4 | 4.7 | 3.6 | – | 4.2 | 3.5 | 4.1 | 4.1 | 3.8 | 5.3 | 5.8 | 5.4 | 7.5 | 6.9 | 7.1 | 5.1 | 6.6 | 6.7 |
Std Deviation (1e-4) | 0.4 | 1.2 | 1.9 | 1.4 | 2.5 | 1.1 | – | 2.5 | 1.8 | 2.1 | 3.2 | 1.8 | 2.6 | 3.4 | 3.1 | 1.9 | 1.9 | 1.2 | 2.6 | 3.9 | 4.1 |
Intensity Change (Log2) | – | – | – | -0.19 | 0.31 | -0.09 | – | 0.15 | -0.11 | 0.1 | 0.11 | 0.01 | 0.46 | 0.59 | 0.51 | 0.96 | 0.86 | 0.88 | 0.41 | 0.78 | 0.82 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | 0 | – | – |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | 0 | – | – |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | 0 | – | – |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | 0 | – | – |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | 0 | – | – |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | 0 | – | – |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | 0 | – | – |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | 0 | – | – |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | 0 | – | – |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | 0 | – | – |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | 0 | – | – |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | 0 | – | – |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | 0 | – | – |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | 0 | – | – |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | 0 | – | – |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | 4.6 | – | – |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | -0.0607 | -0.0832 | -0.0755 | -0.206 | 0.5266 | -0.156 | -0.7347 | -0.5097 | -0.656 | -0.3753 | -0.7257 | -0.5266 | 0.9503 | 0.8411 | 0.7639 | 0.7115 | 0.3315 | 0.9719 |
Actin | 0.0186 | 0.0178 | 0.0214 | 0.0129 | 0.07 | 0.0154 | 0.0393 | 0.0116 | 0.0221 | 0.0437 | 0.008 | 0.0272 | 0.0691 | 0.0148 | 0.0249 | 0.0046 | 0.0285 | 0.0207 |
Bud | 0.002 | 0.0074 | 0.0021 | 0.0034 | 0.011 | 0.001 | 0.0034 | 0.004 | 0.01 | 0.012 | 0.0014 | 0.0072 | 0.0033 | 0.0023 | 0.0023 | 0.0041 | 0.0025 | 0.0011 |
Bud Neck | 0.0063 | 0.0043 | 0.0021 | 0.0025 | 0.0035 | 0.0096 | 0.0085 | 0.0048 | 0.0075 | 0.0021 | 0.0045 | 0.0065 | 0.0126 | 0.0011 | 0.0009 | 0.0011 | 0.0009 | 0.01 |
Bud Periphery | 0.0041 | 0.0138 | 0.0039 | 0.0106 | 0.0119 | 0.0038 | 0.0082 | 0.0088 | 0.0169 | 0.0234 | 0.0019 | 0.0082 | 0.0069 | 0.0061 | 0.0056 | 0.0132 | 0.0051 | 0.0031 |
Bud Site | 0.012 | 0.0721 | 0.0111 | 0.0024 | 0.0063 | 0.0015 | 0.0205 | 0.0486 | 0.0206 | 0.0079 | 0.0027 | 0.0032 | 0.0197 | 0.0383 | 0.0075 | 0.0034 | 0.0031 | 0.0017 |
Cell Periphery | 0.0015 | 0.0086 | 0.0014 | 0.0012 | 0.002 | 0.0016 | 0.0025 | 0.0018 | 0.0035 | 0.0017 | 0.0003 | 0.0008 | 0.0025 | 0.0022 | 0.0013 | 0.0008 | 0.0005 | 0.001 |
Cytoplasm | 0.0722 | 0.0982 | 0.0932 | 0.0854 | 0.0724 | 0.1142 | 0.1045 | 0.1473 | 0.1387 | 0.0967 | 0.2105 | 0.1126 | 0.0938 | 0.1327 | 0.0981 | 0.0895 | 0.0799 | 0.1306 |
Cytoplasmic Foci | 0.1191 | 0.092 | 0.1326 | 0.1524 | 0.0918 | 0.1427 | 0.1361 | 0.1534 | 0.1355 | 0.1417 | 0.1755 | 0.1355 | 0.1213 | 0.093 | 0.0905 | 0.0926 | 0.1085 | 0.1102 |
Eisosomes | 0.0011 | 0.0022 | 0.0011 | 0.0005 | 0.0004 | 0.0005 | 0.0011 | 0.0007 | 0.0012 | 0.0009 | 0.0004 | 0.0006 | 0.0012 | 0.0009 | 0.0007 | 0.0007 | 0.0003 | 0.0007 |
Endoplasmic Reticulum | 0.0079 | 0.0042 | 0.0062 | 0.0044 | 0.0037 | 0.0042 | 0.0054 | 0.0044 | 0.0048 | 0.0074 | 0.0037 | 0.0031 | 0.0084 | 0.0148 | 0.0039 | 0.0039 | 0.0029 | 0.0039 |
Endosome | 0.1317 | 0.0553 | 0.1001 | 0.1341 | 0.1135 | 0.1031 | 0.1276 | 0.1033 | 0.1015 | 0.0768 | 0.1156 | 0.1174 | 0.1102 | 0.0769 | 0.0736 | 0.0762 | 0.1335 | 0.1016 |
Golgi | 0.0319 | 0.0191 | 0.0323 | 0.0641 | 0.0412 | 0.0543 | 0.0382 | 0.0425 | 0.0384 | 0.0171 | 0.0273 | 0.0576 | 0.0333 | 0.0203 | 0.027 | 0.0153 | 0.0421 | 0.0295 |
Lipid Particles | 0.0462 | 0.014 | 0.0135 | 0.0035 | 0.0194 | 0.0277 | 0.0418 | 0.0105 | 0.0152 | 0.02 | 0.0225 | 0.0193 | 0.0497 | 0.0147 | 0.0155 | 0.0032 | 0.0105 | 0.0207 |
Mitochondria | 0.1447 | 0.2207 | 0.2408 | 0.2732 | 0.2192 | 0.2647 | 0.1586 | 0.136 | 0.1659 | 0.2693 | 0.1003 | 0.2515 | 0.0924 | 0.1295 | 0.1892 | 0.3297 | 0.1929 | 0.2266 |
None | 0.304 | 0.3244 | 0.2745 | 0.2018 | 0.2095 | 0.1635 | 0.2243 | 0.2682 | 0.2654 | 0.2162 | 0.2623 | 0.1788 | 0.3038 | 0.4127 | 0.4043 | 0.3319 | 0.3468 | 0.2825 |
Nuclear Periphery | 0.0062 | 0.0012 | 0.0031 | 0.0014 | 0.0015 | 0.0014 | 0.0051 | 0.0018 | 0.0021 | 0.0056 | 0.0028 | 0.0035 | 0.0087 | 0.0018 | 0.0013 | 0.0012 | 0.0021 | 0.0039 |
Nucleolus | 0.0035 | 0.0025 | 0.0011 | 0.0011 | 0.0045 | 0.0007 | 0.0027 | 0.0013 | 0.0014 | 0.0105 | 0.0017 | 0.0012 | 0.004 | 0.001 | 0.0007 | 0.0004 | 0.0004 | 0.0007 |
Nucleus | 0.0032 | 0.0024 | 0.0068 | 0.0019 | 0.0049 | 0.0014 | 0.0024 | 0.0018 | 0.0026 | 0.0043 | 0.003 | 0.0022 | 0.0076 | 0.0016 | 0.0013 | 0.0008 | 0.0012 | 0.0017 |
Peroxisomes | 0.0509 | 0.0228 | 0.0372 | 0.0136 | 0.0557 | 0.0778 | 0.0453 | 0.0367 | 0.027 | 0.0192 | 0.0346 | 0.0481 | 0.0285 | 0.0188 | 0.0381 | 0.0181 | 0.0218 | 0.0386 |
Punctate Nuclear | 0.0068 | 0.0015 | 0.0042 | 0.0016 | 0.0038 | 0.0022 | 0.0045 | 0.0023 | 0.0039 | 0.0052 | 0.0114 | 0.0033 | 0.0074 | 0.0017 | 0.0014 | 0.0005 | 0.0011 | 0.0041 |
Vacuole | 0.0213 | 0.0129 | 0.0082 | 0.0137 | 0.0411 | 0.0058 | 0.016 | 0.0084 | 0.0124 | 0.013 | 0.007 | 0.0079 | 0.0121 | 0.0118 | 0.0087 | 0.005 | 0.01 | 0.0046 |
Vacuole Periphery | 0.0047 | 0.0025 | 0.0033 | 0.0144 | 0.0128 | 0.003 | 0.0042 | 0.0019 | 0.0033 | 0.0054 | 0.0026 | 0.0045 | 0.0035 | 0.003 | 0.0032 | 0.0039 | 0.0053 | 0.0024 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 53.5233 | 44.1661 | 30.9524 | 35.3864 | 39.1461 | 74.0682 | 52.099 | 38.5122 | 49.8206 | 34.8765 |
Translational Efficiency | 1.7152 | 1.295 | 1.744 | 1.9886 | 1.232 | 1.4234 | 1.1631 | 2.3843 | 1.6016 | 1.685 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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