Standard name | |
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Human Ortholog | |
Description | Iron transporter of the mitochondrial carrier family; mediates Fe2+ transport across the inner mitochondrial membrane; active under low-iron conditions; may transport other cations; protein abundance increases in response to DNA replication stress; MRS4 has a paralog, MRS3, that arose from the whole genome duplication |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 9 | 3 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 10 | 8 | 10 | 2 | 7 | 1 | 3 | 12 | 8 | 4 | 8 | 14 |
Bud | 5 | 0 | 3 | 7 | 11 | 9 | 8 | 13 | 13 | 12 | 16 | 12 | 1 | 2 | 3 | 1 | 0 | 1 | 1 | 3 | 0 | 1 | 6 | 8 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 2 | 3 |
Bud Site | 0 | 1 | 0 | 0 | 0 | 4 | 4 | 6 | 4 | 12 | 5 | 7 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 20 | 4 | 5 | 1 | 2 | 4 | 2 | 0 | 2 | 3 | 3 | 7 | 5 | 5 | 5 | 6 | 2 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 7 | 8 | 6 | 8 | 13 | 10 | 14 | 11 | 5 | 11 | 5 | 7 | 5 | 4 | 7 | 23 | 35 | 22 | 3 | 3 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 1 | 2 | 4 | 4 | 3 | 9 | 1 | 1 | 1 | 2 | 2 | 5 | 3 | 5 | 8 | 3 | 3 | 1 | 0 | 1 | 1 | 1 | 0 | 3 |
Endosome | 5 | 5 | 7 | 4 | 7 | 1 | 2 | 2 | 3 | 5 | 0 | 1 | 4 | 4 | 0 | 5 | 7 | 7 | 12 | 12 | 21 | 8 | 15 | 17 |
Golgi | 1 | 49 | 22 | 46 | 5 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 117 | 134 | 189 | 2 | 3 | 1 | 10 | 29 | 30 | 17 | 33 | 33 |
Mitochondria | 77 | 134 | 286 | 229 | 292 | 337 | 532 | 481 | 495 | 475 | 327 | 495 | 116 | 113 | 105 | 5 | 2 | 6 | 68 | 121 | 244 | 124 | 140 | 167 |
Nucleus | 2 | 1 | 0 | 0 | 3 | 7 | 9 | 18 | 5 | 9 | 7 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 |
Nuclear Periphery | 1 | 2 | 9 | 5 | 7 | 21 | 27 | 27 | 47 | 34 | 14 | 40 | 2 | 3 | 5 | 0 | 0 | 1 | 0 | 1 | 3 | 0 | 2 | 5 |
Nucleolus | 10 | 3 | 11 | 6 | 21 | 21 | 33 | 51 | 44 | 39 | 17 | 11 | 7 | 8 | 7 | 1 | 0 | 3 | 0 | 1 | 0 | 0 | 2 | 0 |
Peroxisomes | 0 | 0 | 8 | 3 | 1 | 3 | 1 | 4 | 1 | 4 | 0 | 4 | 1 | 1 | 1 | 0 | 1 | 2 | 2 | 3 | 15 | 7 | 8 | 5 |
SpindlePole | 0 | 0 | 4 | 0 | 3 | 6 | 5 | 13 | 4 | 15 | 2 | 3 | 0 | 2 | 0 | 1 | 0 | 1 | 1 | 1 | 3 | 2 | 2 | 1 |
Vac/Vac Membrane | 9 | 47 | 28 | 20 | 31 | 19 | 48 | 33 | 29 | 44 | 15 | 25 | 72 | 80 | 59 | 9 | 13 | 5 | 2 | 22 | 6 | 12 | 29 | 31 |
Unique Cell Count | 95 | 194 | 313 | 255 | 332 | 375 | 562 | 524 | 522 | 523 | 338 | 514 | 233 | 249 | 278 | 46 | 67 | 44 | 112 | 217 | 343 | 187 | 258 | 299 |
Labelled Cell Count | 118 | 281 | 395 | 340 | 398 | 452 | 688 | 662 | 651 | 664 | 413 | 613 | 345 | 370 | 400 | 57 | 77 | 53 | 112 | 217 | 343 | 187 | 258 | 299 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 6.3 | 9.9 | 7.4 | 8.0 | 6.9 | 5.7 | 5.0 | 5.0 | 4.8 | 4.4 | 4.7 | 4.7 | 15.4 | 17.1 | 18.1 | 7.5 | 7.9 | 7.8 | 9.3 | 9.4 | 9.7 |
Std Deviation (1e-4) | 1.8 | 2.2 | 1.6 | 1.9 | 1.6 | 1.3 | 1.3 | 1.3 | 1.3 | 1.2 | 1.3 | 1.1 | 3.5 | 4.3 | 4.8 | 1.5 | 2.1 | 1.8 | 1.9 | 2.0 | 2.2 |
Intensity Change (Log2) | – | – | – | 0.12 | -0.1 | -0.38 | -0.56 | -0.57 | -0.61 | -0.75 | -0.66 | -0.66 | 1.06 | 1.21 | 1.29 | 0.02 | 0.1 | 0.08 | 0.33 | 0.35 | 0.4 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2.5 | 0 | 2.2 | 0 | 0 | 0 |
Bud | 0 | 2.1 | 1.4 | 0 | 1.6 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0.9 | 1.5 | 0.6 | 0.5 | 0.2 | 0 | 0.2 | -0.4 | 0 | 0 | 0 | 0.5 | 0 | 12.0 | 0 |
Endoplasmic Reticulum | 0 | 0 | 1.1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1.4 | 0 | 0 | 0 |
Endosome | 0 | -0.1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 4.0 | -3.5 | -4.3 | -6.4 | -6.2 | 0 | 0 | 0 | 0 | 11.5 | 12.3 | 15.4 | 0 | 0 | 0 |
Mitochondria | -0.6 | -1.4 | -0.7 | 1.9 | 0.2 | 0 | 0 | 0 | 0 | -10.9 | -11.9 | -13.7 | -13.0 | -15.3 | -12.5 |
Nucleus | 0 | 0 | 0 | 0 | 3.3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | -0.7 | -0.6 | 1.7 | 1.4 | 1.6 | 0 | 0 | 0 | 0 | 0 | -1.4 | -0.9 | 0 | 0 | 0 |
Nucleolus | -0.8 | 1.6 | 1.3 | 1.5 | 3.3 | 0 | 0 | 0 | 0 | -0.3 | -0.2 | -0.7 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | -1.8 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 1.2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | -0.5 | 0.2 | -2.0 | -0.2 | -1.4 | -1.9 | -0.3 | -2.3 | -2.3 | 6.6 | 6.9 | 4.2 | 0 | 2.5 | 0 |
R1 | R2 | R3 | ||||||||||||||||
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G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 4.9403 | 5.9058 | 5.0773 | 4.5779 | 5.8 | 5.2573 | 7.2479 | 7.7793 | 7.0969 | 6.9343 | 7.0114 | 7.6603 | 5.1675 | 6.1312 | 5.3231 | 5.1514 | 5.8434 | 5.7321 |
Actin | 0.0165 | 0.0241 | 0.0199 | 0.0052 | 0.012 | 0.0062 | 0.0296 | 0.0417 | 0.0278 | 0.0295 | 0.0106 | 0.0201 | 0.0095 | 0.0197 | 0.0191 | 0.0133 | 0.0114 | 0.0079 |
Bud | 0.0036 | 0.0146 | 0.0077 | 0.009 | 0.0084 | 0.0027 | 0.0034 | 0.0079 | 0.0061 | 0.009 | 0.01 | 0.003 | 0.0032 | 0.0064 | 0.0074 | 0.005 | 0.007 | 0.0038 |
Bud Neck | 0.0015 | 0.0017 | 0.0032 | 0.0049 | 0.0014 | 0.0039 | 0.0014 | 0.0013 | 0.0033 | 0.0013 | 0.0011 | 0.0021 | 0.0015 | 0.0012 | 0.0012 | 0.0014 | 0.0013 | 0.0032 |
Bud Periphery | 0.0062 | 0.0157 | 0.013 | 0.0292 | 0.0184 | 0.0042 | 0.0061 | 0.0116 | 0.0109 | 0.0205 | 0.0268 | 0.0058 | 0.0048 | 0.0056 | 0.0119 | 0.0076 | 0.0146 | 0.0071 |
Bud Site | 0.0087 | 0.0467 | 0.0221 | 0.0061 | 0.0054 | 0.0024 | 0.0083 | 0.0423 | 0.0128 | 0.0073 | 0.0031 | 0.0021 | 0.0088 | 0.0277 | 0.0116 | 0.0037 | 0.0027 | 0.0028 |
Cell Periphery | 0.0016 | 0.0018 | 0.0019 | 0.003 | 0.0009 | 0.0006 | 0.0014 | 0.0016 | 0.0064 | 0.0023 | 0.0007 | 0.0012 | 0.0021 | 0.001 | 0.0009 | 0.0004 | 0.0004 | 0.0006 |
Cytoplasm | 0.0451 | 0.1025 | 0.0713 | 0.0437 | 0.1099 | 0.0649 | 0.0479 | 0.1007 | 0.0568 | 0.0585 | 0.0583 | 0.0421 | 0.0445 | 0.1172 | 0.0634 | 0.018 | 0.0658 | 0.0495 |
Cytoplasmic Foci | 0.0626 | 0.087 | 0.0733 | 0.0687 | 0.0571 | 0.0719 | 0.0652 | 0.0778 | 0.0789 | 0.0652 | 0.0693 | 0.0611 | 0.0673 | 0.1023 | 0.0657 | 0.042 | 0.0781 | 0.0772 |
Eisosomes | 0.0003 | 0.0003 | 0.0004 | 0.0002 | 0.0004 | 0.0002 | 0.0004 | 0.0004 | 0.0007 | 0.0005 | 0.0003 | 0.0006 | 0.0005 | 0.0002 | 0.0003 | 0.0001 | 0.0002 | 0.0001 |
Endoplasmic Reticulum | 0.0105 | 0.0111 | 0.01 | 0.0087 | 0.0127 | 0.0089 | 0.0112 | 0.0052 | 0.0029 | 0.0085 | 0.0069 | 0.005 | 0.0141 | 0.0058 | 0.0052 | 0.0019 | 0.003 | 0.0089 |
Endosome | 0.2537 | 0.2015 | 0.1768 | 0.2135 | 0.2024 | 0.2255 | 0.2091 | 0.1421 | 0.1154 | 0.1484 | 0.1837 | 0.1485 | 0.2389 | 0.2159 | 0.1668 | 0.1791 | 0.2073 | 0.1891 |
Golgi | 0.1244 | 0.1217 | 0.1245 | 0.1025 | 0.1336 | 0.152 | 0.1674 | 0.162 | 0.1289 | 0.0629 | 0.1552 | 0.1776 | 0.1316 | 0.1602 | 0.1287 | 0.1139 | 0.1703 | 0.1622 |
Lipid Particles | 0.0054 | 0.0029 | 0.0045 | 0.0032 | 0.0074 | 0.003 | 0.0053 | 0.0039 | 0.0036 | 0.0012 | 0.0013 | 0.0022 | 0.0152 | 0.0023 | 0.0019 | 0.0031 | 0.0015 | 0.0033 |
Mitochondria | 0.4135 | 0.3131 | 0.421 | 0.4568 | 0.3748 | 0.4072 | 0.4056 | 0.3732 | 0.5068 | 0.523 | 0.4411 | 0.5051 | 0.4053 | 0.3005 | 0.4827 | 0.5751 | 0.4034 | 0.4583 |
None | 0.0022 | 0.0013 | 0.0027 | 0.0008 | 0.009 | 0.0086 | 0.0013 | 0.0034 | 0.0044 | 0.0018 | 0.0011 | 0.001 | 0.0031 | 0.0015 | 0.0024 | 0.0008 | 0.0009 | 0.0012 |
Nuclear Periphery | 0.0033 | 0.0028 | 0.0022 | 0.003 | 0.0031 | 0.0027 | 0.0033 | 0.0008 | 0.0005 | 0.0013 | 0.0006 | 0.0008 | 0.0084 | 0.0009 | 0.0012 | 0.0006 | 0.0004 | 0.0011 |
Nucleolus | 0.0004 | 0.0006 | 0.0005 | 0.0002 | 0.0013 | 0.0004 | 0.0002 | 0.0003 | 0.0003 | 0.0002 | 0.0001 | 0.0001 | 0.0008 | 0.0002 | 0.0002 | 0.0002 | 0.0001 | 0.0002 |
Nucleus | 0.0012 | 0.0033 | 0.0022 | 0.001 | 0.0024 | 0.0025 | 0.0008 | 0.001 | 0.0007 | 0.0011 | 0.0006 | 0.0006 | 0.0014 | 0.0012 | 0.0009 | 0.0007 | 0.0007 | 0.0006 |
Peroxisomes | 0.0111 | 0.0118 | 0.0182 | 0.011 | 0.0069 | 0.0077 | 0.0138 | 0.013 | 0.0201 | 0.0286 | 0.0062 | 0.0105 | 0.0092 | 0.0144 | 0.0109 | 0.0079 | 0.01 | 0.0092 |
Punctate Nuclear | 0.0008 | 0.0019 | 0.0006 | 0.0002 | 0.0082 | 0.0019 | 0.0003 | 0.0003 | 0.0003 | 0.0003 | 0.0002 | 0.0002 | 0.0024 | 0.0003 | 0.0003 | 0.0002 | 0.0003 | 0.0005 |
Vacuole | 0.0169 | 0.0253 | 0.0166 | 0.0174 | 0.0158 | 0.0129 | 0.0106 | 0.0059 | 0.0089 | 0.021 | 0.016 | 0.0055 | 0.0168 | 0.0101 | 0.0104 | 0.0163 | 0.0153 | 0.0072 |
Vacuole Periphery | 0.0107 | 0.0085 | 0.0074 | 0.0116 | 0.0087 | 0.0098 | 0.0073 | 0.0036 | 0.0036 | 0.0077 | 0.0071 | 0.0049 | 0.0106 | 0.0052 | 0.0067 | 0.0086 | 0.0054 | 0.0062 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 24.1181 | 29.1062 | 23.9978 | 28.0059 | 14.0716 | 28.9257 | 53.4086 | 68.784 | 44.3528 | 34.2358 |
Translational Efficiency | 1.0369 | 1.167 | 1.1054 | 0.7527 | 0.8408 | 1.2492 | 0.9983 | 0.6398 | 1.0369 | 0.8734 |
Dataset | Hit |
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Protein Concentration | ✔ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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