Standard name
Human Ortholog
Description Non-essential small GTPase; member of the Rho/Rac subfamily of Ras-like proteins; likely to be involved in the establishment of cell polarity; has long N-terminal extension that plays an important role in Rho4p function and is shared with Rho4 homologs in other yeasts and filamentous fungi
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0.05 0 0 0.07 0.05 0
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.19 0.46 0.65 0.48 0.31 0.3 0.23 0.2 – 0.19 0.21 0.24 0.63 0.72 0.92 0.28 0.22 0.3 0.23 0.4 0.57 0.37 0.49 0.57
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0.06 0 0.05 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.42 0 0.31 0.42 0.4 0.49 0.74 0.69 – 0.74 0.76 0.66 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.51 0.11 0.09 0.21 0.25 0.34 0.27 0.33 – 0.28 0.23 0.29 0 0.06 0.07 0.51 0.4 0.27 0.53 0.36 0.18 0.15 0.15 0.07
Nuclear Periphery 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.12 0.55 0.08 0.15 0.26 0.09 0.1 0.08 – 0.09 0.08 0.06 0.35 0.3 0.08 0.37 0.41 0.54 0.07 0.11 0.13 0.31 0.18 0.21
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 1 1 0 0 0
Bud 0 0 0 0 1 4 2 11 – 8 1 3 1 0 0 0 1 0 7 5 2 3 4 2
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 1 2 0 0 1 3
Bud Site 0 0 0 0 0 0 1 2 – 5 2 3 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0
Cytoplasm 22 81 89 53 44 50 72 66 – 62 42 81 45 48 69 48 38 63 33 79 95 15 38 78
Endoplasmic Reticulum 0 1 1 1 0 0 0 0 – 0 0 2 1 2 0 11 6 11 1 0 0 0 0 1
Endosome 0 0 0 0 1 0 0 0 – 0 0 2 2 1 0 0 4 11 2 5 1 0 0 1
Golgi 0 2 0 0 0 0 0 0 – 0 0 0 0 0 0 0 1 2 0 1 0 0 0 0
Mitochondria 48 7 43 46 57 81 228 223 – 237 149 228 0 0 0 4 1 4 2 3 4 0 2 3
Nucleus 58 20 13 23 35 56 83 107 – 90 45 101 3 4 5 88 68 57 76 71 30 6 12 9
Nuclear Periphery 1 1 0 0 0 0 0 1 – 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 1 0 0 0 0 0 0 1 – 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 1 – 2 0 0 0 0 0 0 0 0 4 4 3 1 0 3
Vac/Vac Membrane 14 98 11 17 37 15 31 26 – 28 15 21 25 20 6 64 70 114 10 21 21 12 13 28
Unique Cell Count 113 177 137 110 142 165 309 322 319 196 343 71 67 75 174 169 211 146 200 168 41 78 138
Labelled Cell Count 144 210 157 140 175 206 417 438 433 255 441 77 75 80 215 190 262 146 200 168 41 78 138


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.4 5.4 3.7 3.7 3.9 3.9 3.3 3.5 – 3.3 3.2 3.4 6.4 5.4 5.5 7.5 7.9 8.2 – 6.8 6.8
Std Deviation (1e-4) 1.2 1.0 0.6 0.9 1.1 1.3 0.8 1.2 – 1.0 0.9 0.9 2.3 1.5 1.3 1.0 1.2 1.4 – 2.0 2.6
Intensity Change (Log2) – – – 0.01 0.1 0.1 -0.15 -0.08 – -0.13 -0.19 -0.1 0.8 0.57 0.6 1.03 1.11 1.15 – 0.89 0.89


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Cytoplasm -2.7 -5.7 -6.0 -8.5 -9.2 – -9.5 -8.0 -8.6 -0.2 1.0 4.3 -6.6 -7.5 -6.5
Endoplasmic Reticulum 0 0 0 0 0 – 0 0 0 0 0 0 2.5 0 0
Endosome 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 – 0 0 0 -5.3 -5.2 -5.4 -7.1 -7.6 -7.9
Nucleus 2.5 3.4 5.0 4.1 5.3 – 4.4 3.2 4.6 -1.4 -0.9 -0.7 7.7 6.1 4.0
Nuclear Periphery 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Vacuole 1.8 4.0 0.3 0.7 0 – 0.3 -0.1 -0.8 4.9 4.1 0 5.9 6.6 8.7

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.055 0.4349 0.1448 -0.2504 0.3917 0.2686 2.1053 2.6972 2.3527 2.4702 2.1208 2.5586 -0.842 -0.3357 -0.6944 -0.2633 -0.6584 -0.4451
Actin 0.019 0.0004 0.0056 0.0006 0.0032 0.001 0.0053 0.0001 0.0019 0.0011 0.0027 0.0032 0.0018 0.0001 0.0003 0.0002 0.0016 0.0004
Bud 0.0012 0.0006 0.0013 0.0035 0.0002 0.0003 0.0005 0.0002 0.0003 0.0001 0.0001 0.0008 0.0002 0 0.0001 0 0.0001 0.0002
Bud Neck 0.0018 0.0005 0.0005 0.0007 0.0006 0.0019 0.0072 0.0006 0.001 0.0007 0.0008 0.0034 0.0007 0.0003 0.0004 0.0006 0.001 0.0024
Bud Periphery 0.0005 0.0006 0.0003 0.0014 0.0004 0.0002 0.0004 0.0001 0.0004 0.0001 0.0001 0.0017 0.0001 0 0 0 0 0.0002
Bud Site 0.0126 0.0028 0.0024 0.0009 0.0005 0.0022 0.0044 0.0012 0.0017 0.0004 0.0003 0.0005 0.0005 0.0007 0.0003 0.0001 0.0002 0.0003
Cell Periphery 0.0003 0.0001 0.0001 0 0.0001 0 0.0002 0.0001 0.0001 0 0 0 0.0001 0 0 0 0 0
Cytoplasm 0.2741 0.2689 0.2032 0.2195 0.1014 0.2097 0.2337 0.2121 0.2018 0.2295 0.2003 0.18 0.2518 0.18 0.1883 0.1565 0.2005 0.182
Cytoplasmic Foci 0.0234 0.005 0.0078 0.0021 0.0243 0.0066 0.01 0.0025 0.0054 0.0038 0.0075 0.0086 0.0067 0.0026 0.0035 0.0029 0.0068 0.0036
Eisosomes 0.0003 0 0.0001 0 0.0003 0.0001 0.0004 0 0.0001 0.0001 0.0001 0 0.0002 0 0.0001 0 0.0001 0
Endoplasmic Reticulum 0.0063 0.0033 0.0039 0.0014 0.0018 0.0019 0.0041 0.0027 0.0028 0.0036 0.0027 0.0025 0.0043 0.0018 0.0024 0.0014 0.0026 0.002
Endosome 0.0192 0.0035 0.0071 0.0016 0.021 0.0043 0.0042 0.003 0.0043 0.0032 0.0107 0.0061 0.0066 0.0011 0.0022 0.0017 0.0041 0.0022
Golgi 0.0034 0.0002 0.0015 0.0001 0.0082 0.0007 0.0006 0.0001 0.0005 0.0001 0.0047 0.0007 0.0007 0 0.0001 0 0.0003 0.0001
Lipid Particles 0.0085 0.0002 0.0015 0.0001 0.0214 0.0016 0.0014 0.0001 0.0013 0.0004 0.0051 0.0004 0.0023 0.0001 0.0002 0.0001 0.0017 0.0002
Mitochondria 0.0036 0.0011 0.0037 0.0005 0.013 0.0029 0.0008 0.0005 0.001 0.001 0.0102 0.0016 0.001 0.0002 0.0006 0.0005 0.0006 0.0003
None 0.2357 0.2816 0.3024 0.2691 0.3214 0.2144 0.3545 0.1696 0.297 0.3261 0.2064 0.2055 0.3033 0.1283 0.269 0.1842 0.2294 0.1614
Nuclear Periphery 0.0221 0.0131 0.0184 0.0129 0.0173 0.0115 0.0107 0.008 0.0116 0.0276 0.0099 0.0084 0.0121 0.0068 0.0129 0.0104 0.0128 0.0108
Nucleolus 0.0035 0.0025 0.003 0.0027 0.0033 0.0038 0.0026 0.0016 0.003 0.0027 0.0026 0.0034 0.0025 0.0015 0.0018 0.0029 0.0036 0.003
Nucleus 0.3221 0.3882 0.3912 0.426 0.4083 0.4854 0.3353 0.5801 0.4296 0.3719 0.4794 0.5336 0.3807 0.6662 0.4948 0.6 0.4891 0.6005
Peroxisomes 0.0119 0.0004 0.0054 0.0001 0.0154 0.0018 0.003 0.0001 0.0022 0.0003 0.0163 0.0007 0.0014 0 0.0001 0.0001 0.001 0.0001
Punctate Nuclear 0.0262 0.0239 0.0378 0.056 0.0343 0.0484 0.0175 0.0153 0.0319 0.0253 0.0376 0.037 0.0198 0.0091 0.0218 0.0375 0.0433 0.0293
Vacuole 0.0034 0.0027 0.002 0.0006 0.0025 0.001 0.0029 0.0018 0.0017 0.0015 0.0017 0.0015 0.0028 0.0009 0.001 0.0006 0.0009 0.0009
Vacuole Periphery 0.0008 0.0004 0.0005 0.0002 0.0012 0.0003 0.0004 0.0002 0.0003 0.0006 0.0006 0.0003 0.0005 0.0001 0.0002 0.0001 0.0002 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 7.5576 12.386 5.0132 9.9584 6.5325 3.7767 11.1004 9.6051 10.2281 10.1119
Translational Efficiency 3.233 1.7427 3.7639 2.2195 2.7589 5.1221 2.3684 2.3554 2.0724 2.2595

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Non-essential small GTPase; member of the Rho/Rac subfamily of Ras-like proteins; likely to be involved in the establishment of cell polarity; has long N-terminal extension that plays an important role in Rho4p function and is shared with Rho4 homologs in other yeasts and filamentous fungi
Localization
Cell Percentages nucleus (23%), cytoplasm (11%), mixed (53%)
Cell Cycle Regulation Yes
cytoplasm - MA (0.00091)
Subcompartmental Group N/A

Rho4

Rho4


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Rho4-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available