Standard name
Human Ortholog
Description Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S21, no bacterial homolog; RPS21A has a paralog, RPS21B, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0.06 0 0.09 0.09 0.08 0.08 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.99 1.0 1.0 0.98 0.93 0.89 0.77 0.77 0.73 0.67 0.7 0.65 0.99 1.0 1.0 0.99 0.99 0.98 0.91 0.96 0.96 0.93 0.89 0.89
Endoplasmic Reticulum 0 0 0 0 0.07 0.09 0.17 0.18 0.2 0.21 0.2 0.26 0 0 0 0.06 0 0.05 0 0 0 0 0.05 0.07
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0.07 0.06 0.05 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 3 1 6 3 17 12 10 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 1 0 2 3 0 2 2 0 0 0 1 0 0 0 0 0 0 1 1 2
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 2 0 1 3 15 9 33 24 28 39 24 24 2 3 1 1 1 1 0 0 0 0 1 0
Cytoplasm 153 155 132 256 352 215 403 444 235 285 222 204 273 472 486 231 198 190 124 147 125 262 332 337
Endoplasmic Reticulum 0 0 0 4 26 21 90 101 65 88 63 83 2 4 2 13 8 9 3 1 2 11 17 24
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0
Golgi 0 0 0 0 0 0 16 15 5 31 19 15 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 4 5 4 4 0 0 0 1 1 1 1 0 0 1 1 1
Nucleus 0 0 0 0 1 0 1 0 0 1 0 0 0 0 0 1 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 1 0 0 0 0 1 0 0 0 6 1 0 0 0 0 0 1 0 0 1 0 0 8 3
Unique Cell Count 155 155 132 260 380 242 524 577 321 428 316 315 276 474 488 233 199 194 137 154 130 283 372 378
Labelled Cell Count 156 155 133 263 395 249 546 593 340 474 347 340 277 479 490 247 209 201 137 154 130 283 372 378


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 121.0 140.6 125.8 122.1 99.1 58.8 68.2 57.8 53.4 50.5 47.3 46.0 138.0 143.2 140.1 173.5 152.2 197.5 134.1 136.0 142.8
Std Deviation (1e-4) 31.1 30.1 15.7 23.3 15.6 16.8 14.0 12.3 12.7 10.8 10.6 9.7 28.0 26.7 29.2 44.0 47.1 50.7 26.1 33.1 35.2
Intensity Change (Log2) – – – -0.04 -0.34 -1.1 -0.88 -1.12 -1.24 -1.32 -1.41 -1.45 0.13 0.19 0.16 0.46 0.27 0.65 0.09 0.11 0.18


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 2.6 0 3.1 3.3 2.9 2.9 0 0 0 0 0 0
Cytoplasm -1.4 -3.2 -4.0 -6.1 -6.1 -6.6 -7.7 -7.0 -7.9 -1.2 -0.7 -0.7 -1.1 -0.8 -1.7
Endoplasmic Reticulum 0 3.1 3.5 5.1 5.2 5.6 5.7 5.5 6.5 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 3.2 2.9 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 93.1396 141.843 127.9197 124.2013 123.2126 116.453 126.3882 180.4964 168.9974 164.1891 147.3275 160.2267 121.209 167.2508 148.605 143.1727 156.8985 138.8159
Actin 0.0069 0.0007 0.0015 0.0036 0.0007 0.0163 0.0146 0.0005 0.0038 0.0003 0.0009 0.0003 0.0091 0.0005 0.0016 0.001 0.001 0.0788
Bud 0.003 0.0009 0.0013 0.0019 0.0014 0.0117 0.0023 0.0006 0.0017 0.0023 0.0006 0.0009 0.0029 0.001 0.0026 0.0026 0.003 0.0035
Bud Neck 0.0009 0.0007 0.0011 0.0024 0.002 0.0042 0.0007 0.0006 0.0057 0.0006 0.0024 0.0017 0.0023 0.0007 0.0011 0.0012 0.0009 0.0018
Bud Periphery 0.0038 0.0006 0.0013 0.001 0.0008 0.0094 0.0029 0.0005 0.0009 0.001 0.0003 0.0016 0.0053 0.0006 0.0019 0.0011 0.0012 0.0033
Bud Site 0.0018 0.0009 0.0006 0.0004 0.0002 0.002 0.0019 0.0009 0.0022 0.0003 0.0003 0.0001 0.0026 0.0012 0.0052 0.0005 0.0002 0.0008
Cell Periphery 0.0347 0.0196 0.0102 0.0099 0.0059 0.035 0.0487 0.0326 0.0341 0.0186 0.0393 0.0112 0.0374 0.0262 0.023 0.012 0.0024 0.0039
Cytoplasm 0.5536 0.8875 0.7972 0.563 0.8465 0.6216 0.5003 0.8539 0.7652 0.6202 0.7125 0.8193 0.5771 0.8889 0.7225 0.5515 0.7906 0.7325
Cytoplasmic Foci 0.0167 0.0011 0.012 0.0016 0.0126 0.0089 0.0256 0.0007 0.008 0.0064 0.0519 0.0012 0.0198 0.0009 0.0039 0.0045 0.0021 0.0078
Eisosomes 0.0007 0.0014 0 0.003 0 0.0013 0.002 0 0.0002 0.0001 0.0001 0 0.0014 0 0.0001 0 0 0.0004
Endoplasmic Reticulum 0.0555 0.0079 0.0225 0.0321 0.0042 0.0275 0.0197 0.0088 0.0051 0.0027 0.0018 0.0056 0.0426 0.0107 0.0073 0.0026 0.0951 0.0159
Endosome 0.0457 0.003 0.0103 0.012 0.0209 0.0192 0.0103 0.0009 0.0027 0.0042 0.0047 0.0032 0.0076 0.0019 0.0043 0.0221 0.0065 0.0331
Golgi 0.0096 0.0005 0.0014 0.0042 0.0029 0.0072 0.0024 0.0002 0.0011 0.0002 0.001 0.0001 0.0018 0.0002 0.0006 0.0017 0.0015 0.0162
Lipid Particles 0.0104 0.0009 0.0041 0.0005 0.0009 0.0037 0.0114 0.0016 0.0027 0.0012 0.0188 0.0004 0.0086 0.001 0.0017 0.0022 0.0001 0.0019
Mitochondria 0.015 0.0009 0.0012 0.0015 0.0004 0.0272 0.0175 0.0001 0.0017 0.0052 0.0007 0.0002 0.0229 0.0004 0.0041 0.0021 0.0022 0.0184
None 0.0111 0.0003 0.0014 0.0009 0.0003 0.0023 0.0217 0.0003 0.0025 0.0105 0.0012 0.0003 0.022 0.0002 0.0035 0.0006 0.0004 0.0016
Nuclear Periphery 0.0421 0.0066 0.0047 0.0269 0.0063 0.0266 0.0379 0.0057 0.0115 0.0099 0.0132 0.0064 0.0396 0.0036 0.0074 0.0128 0.0048 0.0105
Nucleolus 0.0037 0.0003 0.0008 0.0005 0.0003 0.0022 0.0282 0.0006 0.0036 0.0034 0.003 0.0002 0.0073 0.0003 0.0104 0.0016 0.0004 0.0004
Nucleus 0.0114 0.0023 0.0031 0.0058 0.0049 0.0094 0.0317 0.004 0.0094 0.0099 0.0088 0.0053 0.0163 0.0037 0.0109 0.011 0.0073 0.005
Peroxisomes 0.0026 0 0.0004 0.0001 0.0001 0.001 0.0013 0 0.0003 0.0005 0.0013 0 0.0021 0 0.0007 0.0004 0.0001 0.0009
Punctate Nuclear 0.0033 0 0.0003 0.0002 0.0001 0.0003 0.0065 0 0.0004 0.0002 0.0011 0.0001 0.0031 0 0.0157 0.0001 0.0001 0.0006
Vacuole 0.136 0.0583 0.0986 0.2503 0.0776 0.092 0.1732 0.0831 0.12 0.2491 0.1137 0.1282 0.1341 0.0523 0.1454 0.266 0.0612 0.0357
Vacuole Periphery 0.0314 0.0057 0.026 0.0783 0.011 0.071 0.0391 0.0044 0.0172 0.0532 0.0225 0.0137 0.0342 0.0055 0.026 0.1024 0.019 0.027

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 1178.6366 1259.1241 1563.6765 1259.604 1211.1432 1449.5052 1293.8767 1114.2282 1383.5057 1253.4662
Translational Efficiency 1.5874 1.7603 1.6601 2.1529 2.1837 1.9849 1.9338 2.4719 1.9565 2.1661

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S21, no bacterial homolog; RPS21A has a paralog, RPS21B, that arose from the whole genome duplication
Localization
Cell Percentages cytoplasm (100%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-1

Rps21a

Rps21a


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Rps21a-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available