Standard name | |
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Human Ortholog | |
Description | TFIIE small subunit; involved in RNA polymerase II transcription initiation |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 1 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 1 | 5 | 5 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 2 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Bud Site | 1 | 0 | 0 | 0 | 0 | 1 | 7 | 5 | 7 | 2 | 7 | 7 | 0 | 1 | 0 | 1 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 4 | 2 | 0 | 4 | 21 | 13 | 14 | 8 | 6 | 4 | 6 | 6 | 13 | 15 | 57 | 14 | 1 | 5 | 0 | 0 | 0 | 1 | 1 | 0 |
Endoplasmic Reticulum | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 2 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 4 | 1 | 0 | 0 | 1 | 0 | 0 | 2 | 3 | 2 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 1 |
Mitochondria | 10 | 18 | 0 | 1 | 0 | 6 | 35 | 11 | 14 | 21 | 29 | 19 | 0 | 0 | 0 | 6 | 1 | 8 | 1 | 0 | 0 | 1 | 3 | 0 |
Nucleus | 130 | 66 | 28 | 88 | 310 | 251 | 370 | 369 | 225 | 231 | 223 | 266 | 316 | 511 | 580 | 173 | 42 | 177 | 130 | 81 | 67 | 263 | 422 | 549 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 5 | 2 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 3 |
Nucleolus | 44 | 19 | 5 | 12 | 12 | 27 | 76 | 69 | 69 | 58 | 91 | 56 | 28 | 39 | 32 | 20 | 6 | 23 | 18 | 2 | 7 | 24 | 39 | 63 |
Peroxisomes | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 4 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 1 | 0 | 1 | 0 | 6 | 11 | 1 | 0 | 0 | 0 | 0 | 1 | 2 | 1 | 1 |
Vac/Vac Membrane | 0 | 3 | 1 | 0 | 0 | 0 | 2 | 2 | 1 | 0 | 0 | 3 | 4 | 11 | 6 | 15 | 1 | 1 | 5 | 2 | 0 | 10 | 23 | 32 |
Unique Cell Count | 154 | 82 | 31 | 99 | 342 | 281 | 455 | 420 | 276 | 274 | 265 | 314 | 345 | 552 | 666 | 217 | 50 | 201 | 163 | 89 | 79 | 320 | 507 | 668 |
Labelled Cell Count | 192 | 108 | 34 | 105 | 344 | 298 | 512 | 473 | 325 | 321 | 358 | 360 | 362 | 587 | 694 | 232 | 51 | 215 | 163 | 89 | 79 | 320 | 507 | 668 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 8.2 | 7.7 | 9.1 | 9.5 | 10.0 | 8.4 | 7.5 | 7.8 | 7.1 | 7.1 | 6.5 | 7.2 | 12.9 | 12.5 | 12.0 | 14.4 | 15.3 | 15.8 | 10.5 | 10.7 | 10.9 |
Std Deviation (1e-4) | 1.8 | 2.3 | 1.4 | 3.1 | 2.1 | 1.9 | 1.8 | 1.8 | 1.7 | 1.6 | 1.5 | 1.6 | 2.8 | 2.9 | 3.2 | 4.6 | 4.6 | 4.4 | 2.3 | 2.4 | 2.1 |
Intensity Change (Log2) | – | – | – | 0.06 | 0.13 | -0.12 | -0.28 | -0.22 | -0.35 | -0.35 | -0.5 | -0.34 | 0.5 | 0.45 | 0.4 | 0.66 | 0.75 | 0.79 | 0.2 | 0.24 | 0.25 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | -0.2 | 0.1 | -0.2 | -1.3 | -0.4 | -1.2 | -0.9 | -0.9 | -0.8 | 0.2 | 0.5 | -0.5 | -1.4 | -0.8 | -0.4 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0.1 | 0 | 1.1 | 0.7 | 2.0 | 0.2 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 5.6044 | 5.753 | 5.5951 | 4.4698 | 4.9259 | 5.1935 | 5.2628 | 6.3543 | 6.1017 | 5.3356 | 5.679 | 6.4013 | 5.0308 | 5.8433 | 5.3054 | 4.6674 | 7.5592 | 5.1505 |
Actin | 0.0069 | 0.0001 | 0.0014 | 0 | 0.0092 | 0.0001 | 0.0148 | 0 | 0.0012 | 0 | 0.0021 | 0 | 0.0285 | 0 | 0.0125 | 0.0001 | 0.0003 | 0.0035 |
Bud | 0.0004 | 0.0001 | 0.0006 | 0 | 0.0012 | 0.0001 | 0.0018 | 0.0001 | 0.0002 | 0 | 0.0017 | 0.0001 | 0.0007 | 0 | 0.0004 | 0.0002 | 0.0012 | 0.0002 |
Bud Neck | 0.0011 | 0.0004 | 0.0006 | 0.0004 | 0.0011 | 0.001 | 0.0008 | 0.0005 | 0.0002 | 0.0005 | 0.0034 | 0.0012 | 0.0019 | 0.0001 | 0.0004 | 0.0007 | 0.0034 | 0.0008 |
Bud Periphery | 0.0004 | 0.0001 | 0.0007 | 0 | 0.0026 | 0.0002 | 0.0035 | 0.0001 | 0.0003 | 0.0001 | 0.0022 | 0.0004 | 0.0021 | 0.0001 | 0.0004 | 0.0003 | 0.0086 | 0.0005 |
Bud Site | 0.0112 | 0.0004 | 0.0032 | 0.0001 | 0.0016 | 0.0003 | 0.0084 | 0.0014 | 0.0004 | 0.0001 | 0.0028 | 0.0002 | 0.0028 | 0.0001 | 0.0025 | 0.0004 | 0.0024 | 0.0003 |
Cell Periphery | 0.0003 | 0.0001 | 0.0004 | 0 | 0.0004 | 0.0002 | 0.0006 | 0.0001 | 0.0001 | 0 | 0.0009 | 0.0003 | 0.0012 | 0.0001 | 0.0001 | 0.0002 | 0.0061 | 0.0002 |
Cytoplasm | 0.0219 | 0.0091 | 0.01 | 0 | 0.0002 | 0.0016 | 0.0136 | 0.0001 | 0.0021 | 0 | 0.0214 | 0 | 0.0245 | 0.0007 | 0.0009 | 0.0069 | 0.0001 | 0.0004 |
Cytoplasmic Foci | 0.0645 | 0.0022 | 0.0131 | 0 | 0.0009 | 0.0053 | 0.012 | 0 | 0.0019 | 0 | 0.0088 | 0 | 0.0477 | 0 | 0.0159 | 0.0002 | 0.0005 | 0.0041 |
Eisosomes | 0.0002 | 0 | 0 | 0 | 0.0008 | 0 | 0.0005 | 0 | 0 | 0 | 0.0001 | 0 | 0.0002 | 0 | 0 | 0 | 0.0001 | 0 |
Endoplasmic Reticulum | 0.0061 | 0.0016 | 0.0015 | 0 | 0.0012 | 0.0012 | 0.0051 | 0 | 0.0007 | 0 | 0.0033 | 0 | 0.0071 | 0.0001 | 0.0005 | 0.0002 | 0.0003 | 0.0008 |
Endosome | 0.0409 | 0.0031 | 0.0251 | 0 | 0.0066 | 0.0037 | 0.0378 | 0 | 0.0074 | 0 | 0.026 | 0.0001 | 0.0901 | 0.0004 | 0.0086 | 0.0006 | 0.0016 | 0.0391 |
Golgi | 0.009 | 0.0001 | 0.0068 | 0 | 0.0165 | 0.0004 | 0.0092 | 0 | 0.0025 | 0 | 0.0025 | 0 | 0.016 | 0 | 0.0149 | 0 | 0.0004 | 0.0187 |
Lipid Particles | 0.0645 | 0.0008 | 0.0174 | 0 | 0.0411 | 0.0048 | 0.0288 | 0 | 0.0155 | 0 | 0.0044 | 0.0001 | 0.0676 | 0 | 0.0086 | 0.0001 | 0.0054 | 0.0111 |
Mitochondria | 0.0358 | 0.0002 | 0.0023 | 0.0002 | 0.0345 | 0.0002 | 0.0324 | 0.0002 | 0.0099 | 0.0001 | 0.0068 | 0.0001 | 0.0042 | 0.0003 | 0.0035 | 0.0009 | 0.0149 | 0.0069 |
None | 0.0041 | 0.0031 | 0.0004 | 0 | 0.0007 | 0.0006 | 0.0082 | 0 | 0.0007 | 0 | 0.002 | 0 | 0.0144 | 0.0001 | 0.0003 | 0.0326 | 0.0003 | 0.0001 |
Nuclear Periphery | 0.043 | 0.007 | 0.0074 | 0.003 | 0.0071 | 0.0063 | 0.0588 | 0.0038 | 0.0179 | 0.0012 | 0.0588 | 0.0002 | 0.026 | 0.0093 | 0.0055 | 0.0051 | 0.0013 | 0.0018 |
Nucleolus | 0.1006 | 0.1503 | 0.1406 | 0.2203 | 0.3867 | 0.4821 | 0.0959 | 0.0698 | 0.0992 | 0.1984 | 0.3852 | 0.433 | 0.1114 | 0.0635 | 0.1101 | 0.1981 | 0.7166 | 0.4444 |
Nucleus | 0.4991 | 0.8171 | 0.7406 | 0.7753 | 0.4777 | 0.4744 | 0.6217 | 0.9173 | 0.8259 | 0.7975 | 0.4262 | 0.5609 | 0.4751 | 0.9185 | 0.8056 | 0.735 | 0.198 | 0.4567 |
Peroxisomes | 0.0138 | 0.0001 | 0.0031 | 0 | 0.0021 | 0.0001 | 0.0101 | 0 | 0.0015 | 0 | 0.0016 | 0 | 0.0156 | 0 | 0.0036 | 0 | 0.0008 | 0.0016 |
Punctate Nuclear | 0.0453 | 0.0017 | 0.004 | 0.0004 | 0.0016 | 0.0157 | 0.0189 | 0.0058 | 0.0064 | 0.0018 | 0.0256 | 0.0004 | 0.0295 | 0.0006 | 0.0035 | 0.0091 | 0.0011 | 0.0006 |
Vacuole | 0.0129 | 0.0019 | 0.0169 | 0.0001 | 0.002 | 0.0012 | 0.0074 | 0.0003 | 0.0019 | 0.0002 | 0.0091 | 0.0022 | 0.0247 | 0.0042 | 0.0009 | 0.0069 | 0.0244 | 0.0042 |
Vacuole Periphery | 0.018 | 0.0004 | 0.0038 | 0.0001 | 0.0042 | 0.0005 | 0.0098 | 0.0002 | 0.0041 | 0.0001 | 0.005 | 0.0006 | 0.0088 | 0.0018 | 0.001 | 0.0026 | 0.0121 | 0.0039 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 30.7433 | 23.9848 | 27.4382 | 34.6005 | 25.3655 | 44.6927 | 36.1239 | 38.8736 | 39.6934 | 31.9761 |
Translational Efficiency | 2.1001 | 2.2028 | 1.9366 | 2.0701 | 2.5351 | 1.5371 | 1.3432 | 1.5649 | 1.6273 | 1.7747 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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