ORF | |
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Human Ortholog | |
Description | Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 1 | 4 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 7 | 1 | 1 | 6 | 3 | 5 | 4 | 3 | 3 |
Bud | 2 | 2 | 3 | 4 | 6 | 5 | 21 | 9 | 13 | 13 | 17 | 8 | 2 | 0 | 0 | 4 | 7 | 14 | 4 | 1 | 2 | 2 | 2 | 4 |
Bud Neck | 2 | 0 | 12 | 7 | 9 | 5 | 5 | 6 | 4 | 6 | 2 | 1 | 4 | 1 | 1 | 1 | 1 | 2 | 1 | 3 | 2 | 1 | 1 | 9 |
Bud Site | 1 | 0 | 0 | 0 | 0 | 0 | 5 | 3 | 1 | 7 | 3 | 2 | 0 | 0 | 0 | 1 | 1 | 3 | – | – | – | – | – | – |
Cell Periphery | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 2 | 4 | 1 | 1 | 0 | 0 | 1 | 21 | 18 | 9 | 2 | 0 | 3 | 0 | 1 | 1 |
Cytoplasm | 185 | 86 | 147 | 111 | 174 | 115 | 147 | 132 | 125 | 127 | 121 | 102 | 164 | 192 | 203 | 65 | 45 | 31 | 91 | 52 | 51 | 55 | 91 | 104 |
Endoplasmic Reticulum | 4 | 5 | 0 | 0 | 0 | 1 | 0 | 2 | 0 | 2 | 0 | 1 | 0 | 1 | 4 | 12 | 7 | 6 | 5 | 1 | 1 | 0 | 1 | 0 |
Endosome | 3 | 0 | 1 | 9 | 3 | 6 | 0 | 4 | 2 | 0 | 0 | 4 | 2 | 5 | 7 | 35 | 62 | 66 | 18 | 10 | 10 | 3 | 12 | 10 |
Golgi | 1 | 0 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 11 | 7 | 1 | 2 | 0 | 1 | 0 | 2 | 2 |
Mitochondria | 108 | 26 | 15 | 19 | 24 | 90 | 151 | 124 | 180 | 205 | 226 | 153 | 8 | 5 | 1 | 29 | 18 | 25 | 138 | 70 | 107 | 57 | 97 | 61 |
Nucleus | 0 | 0 | 0 | 0 | 1 | 1 | 8 | 7 | 2 | 6 | 2 | 3 | 0 | 0 | 0 | 8 | 4 | 5 | 0 | 1 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 4 | 1 | 0 | 0 | 1 | 1 | 2 | 2 | 0 | 2 | 0 | 0 | 0 | 0 | 1 | 0 | 2 | 0 | 1 | 0 | 1 | 1 | 0 | 0 |
Nucleolus | 1 | 0 | 0 | 2 | 0 | 1 | 1 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 4 | 0 | 0 | 0 | 0 | 1 | 1 | 0 |
Peroxisomes | 0 | 0 | 3 | 1 | 3 | 3 | 0 | 3 | 0 | 0 | 0 | 1 | 2 | 2 | 9 | 34 | 36 | 75 | 3 | 0 | 2 | 1 | 4 | 1 |
SpindlePole | 9 | 0 | 16 | 19 | 17 | 14 | 21 | 26 | 15 | 17 | 15 | 23 | 7 | 6 | 6 | 101 | 53 | 55 | 13 | 0 | 7 | 4 | 19 | 10 |
Vac/Vac Membrane | 6 | 4 | 0 | 2 | 7 | 8 | 8 | 5 | 4 | 9 | 8 | 7 | 3 | 2 | 2 | 45 | 36 | 35 | 7 | 12 | 4 | 5 | 9 | 8 |
Unique Cell Count | 293 | 119 | 187 | 158 | 221 | 214 | 294 | 267 | 289 | 326 | 321 | 266 | 181 | 210 | 230 | 295 | 224 | 226 | 308 | 160 | 213 | 147 | 256 | 223 |
Labelled Cell Count | 326 | 126 | 203 | 175 | 245 | 251 | 370 | 323 | 350 | 398 | 395 | 307 | 192 | 215 | 235 | 375 | 302 | 328 | 308 | 160 | 213 | 147 | 256 | 223 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 3.1 | 4.3 | 4.5 | 4.6 | 4.6 | 3.8 | 3.4 | 4.4 | 3.5 | 3.6 | 3.3 | 3.7 | 4.5 | 5.0 | 5.1 | 6.3 | 6.0 | 5.5 | 4.5 | 4.5 | 5.1 |
Std Deviation (1e-4) | 0.6 | 0.7 | 1.3 | 2.1 | 2.1 | 1.4 | 1.3 | 1.9 | 1.5 | 1.5 | 1.6 | 1.2 | 0.9 | 1.6 | 1.4 | 1.3 | 1.7 | 1.3 | 1.2 | 1.3 | 1.7 |
Intensity Change (Log2) | – | – | – | 0.01 | 0.01 | -0.25 | -0.42 | -0.05 | -0.39 | -0.34 | -0.47 | -0.29 | -0.0 | 0.13 | 0.18 | 0.47 | 0.4 | 0.28 | -0.01 | -0.01 | 0.18 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | 5.6 | 5.7 | 5.6 |
R1 | R2 | R3 | ||||||||||||||||
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G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 0.6359 | 0.5824 | 0.5281 | 0.3186 | 0.1028 | 0.4637 | -0.516 | -0.3177 | -0.3292 | -0.886 | 0.021 | -0.3079 | -0.5934 | -0.7061 | -1.0166 | 0.6253 | -0.3265 | -1.0115 |
Actin | 0.0376 | 0.01 | 0.0126 | 0.0215 | 0.0612 | 0.0102 | 0.0227 | 0.0018 | 0.014 | 0.0004 | 0.0079 | 0.009 | 0.0787 | 0.0452 | 0.0653 | 0.2179 | 0.0269 | 0.0235 |
Bud | 0.0048 | 0.0028 | 0.0013 | 0.0026 | 0.0026 | 0.0024 | 0.0012 | 0.0028 | 0.0015 | 0.0001 | 0.002 | 0.001 | 0.0024 | 0.0012 | 0.001 | 0.0052 | 0.0008 | 0.0004 |
Bud Neck | 0.005 | 0.0003 | 0.0021 | 0.0193 | 0.0008 | 0.0058 | 0.0042 | 0.0024 | 0.0022 | 0.0001 | 0.0007 | 0.0074 | 0.0029 | 0.0179 | 0.0014 | 0.0538 | 0.0037 | 0.0011 |
Bud Periphery | 0.0106 | 0.0037 | 0.0094 | 0.0098 | 0.0096 | 0.0107 | 0.0026 | 0.0078 | 0.006 | 0.0005 | 0.0085 | 0.0035 | 0.0041 | 0.0019 | 0.0034 | 0.0078 | 0.0012 | 0.0008 |
Bud Site | 0.015 | 0.008 | 0.0034 | 0.0128 | 0.002 | 0.0012 | 0.005 | 0.0264 | 0.0073 | 0.0001 | 0.0008 | 0.0007 | 0.0105 | 0.016 | 0.0071 | 0.0704 | 0.0023 | 0.001 |
Cell Periphery | 0.0011 | 0.0007 | 0.0006 | 0.0027 | 0.0005 | 0.0009 | 0.0012 | 0.0024 | 0.002 | 0.0001 | 0.0004 | 0.0006 | 0.0014 | 0.0029 | 0.0021 | 0.0021 | 0.0007 | 0.0007 |
Cytoplasm | 0.0522 | 0.0636 | 0.0528 | 0.0817 | 0.0837 | 0.0866 | 0.0657 | 0.0866 | 0.0919 | 0.0299 | 0.0422 | 0.0826 | 0.0788 | 0.1888 | 0.123 | 0.0388 | 0.1248 | 0.1863 |
Cytoplasmic Foci | 0.0788 | 0.0552 | 0.0805 | 0.098 | 0.0854 | 0.0888 | 0.0986 | 0.0831 | 0.0857 | 0.0081 | 0.0493 | 0.0612 | 0.1146 | 0.0722 | 0.1125 | 0.0992 | 0.1631 | 0.0646 |
Eisosomes | 0.0011 | 0.0007 | 0.0013 | 0.0005 | 0.0009 | 0.0005 | 0.0011 | 0.0012 | 0.0009 | 0.0001 | 0.0005 | 0.0004 | 0.0019 | 0.0011 | 0.0018 | 0.0032 | 0.0007 | 0.0009 |
Endoplasmic Reticulum | 0.0076 | 0.0019 | 0.0024 | 0.0018 | 0.0023 | 0.0027 | 0.006 | 0.0032 | 0.006 | 0.0003 | 0.0019 | 0.0023 | 0.0208 | 0.0049 | 0.0046 | 0.0024 | 0.0091 | 0.0057 |
Endosome | 0.0569 | 0.0465 | 0.0483 | 0.0417 | 0.0407 | 0.0403 | 0.0596 | 0.0492 | 0.0458 | 0.005 | 0.0498 | 0.0319 | 0.0778 | 0.0568 | 0.0372 | 0.0273 | 0.1447 | 0.0467 |
Golgi | 0.0185 | 0.0201 | 0.0146 | 0.0174 | 0.0376 | 0.0162 | 0.021 | 0.0132 | 0.0116 | 0.0043 | 0.0154 | 0.0096 | 0.0241 | 0.0332 | 0.0124 | 0.043 | 0.068 | 0.0313 |
Lipid Particles | 0.0296 | 0.0059 | 0.017 | 0.0122 | 0.0178 | 0.0171 | 0.0294 | 0.0056 | 0.0153 | 0.0013 | 0.0628 | 0.0119 | 0.0623 | 0.005 | 0.0194 | 0.0231 | 0.0534 | 0.0287 |
Mitochondria | 0.0973 | 0.1526 | 0.0982 | 0.1545 | 0.1315 | 0.1518 | 0.0502 | 0.1056 | 0.0941 | 0.2674 | 0.2771 | 0.0707 | 0.0241 | 0.0794 | 0.0925 | 0.0435 | 0.1306 | 0.0679 |
None | 0.5163 | 0.5713 | 0.5841 | 0.4627 | 0.4753 | 0.5052 | 0.5622 | 0.5715 | 0.5735 | 0.6761 | 0.4155 | 0.6659 | 0.4458 | 0.447 | 0.4857 | 0.2933 | 0.1961 | 0.4958 |
Nuclear Periphery | 0.0068 | 0.0007 | 0.0051 | 0.0011 | 0.0046 | 0.0023 | 0.0086 | 0.0008 | 0.0035 | 0.0001 | 0.0055 | 0.0069 | 0.008 | 0.0012 | 0.0011 | 0.0011 | 0.0057 | 0.002 |
Nucleolus | 0.0049 | 0.0011 | 0.0009 | 0.001 | 0.0008 | 0.0006 | 0.0015 | 0.0017 | 0.0015 | 0.0003 | 0.0012 | 0.0005 | 0.002 | 0.0007 | 0.0008 | 0.0013 | 0.0009 | 0.0011 |
Nucleus | 0.0035 | 0.0017 | 0.0014 | 0.0012 | 0.0024 | 0.0011 | 0.0037 | 0.0024 | 0.0022 | 0.0004 | 0.0015 | 0.0011 | 0.0037 | 0.0012 | 0.0011 | 0.001 | 0.0015 | 0.0017 |
Peroxisomes | 0.0382 | 0.0463 | 0.0521 | 0.0454 | 0.0292 | 0.0471 | 0.0365 | 0.0176 | 0.0201 | 0.0041 | 0.0355 | 0.0235 | 0.0188 | 0.0134 | 0.0215 | 0.0564 | 0.0136 | 0.0323 |
Punctate Nuclear | 0.0052 | 0.0009 | 0.002 | 0.0021 | 0.007 | 0.002 | 0.0062 | 0.0036 | 0.0042 | 0.0002 | 0.0149 | 0.0037 | 0.0101 | 0.0024 | 0.0023 | 0.0046 | 0.0456 | 0.002 |
Vacuole | 0.0065 | 0.0048 | 0.0073 | 0.0065 | 0.0023 | 0.0043 | 0.0105 | 0.0093 | 0.0087 | 0.0007 | 0.0033 | 0.004 | 0.0051 | 0.0061 | 0.0029 | 0.0035 | 0.0038 | 0.0043 |
Vacuole Periphery | 0.0024 | 0.0012 | 0.0022 | 0.0033 | 0.0015 | 0.0022 | 0.0025 | 0.0019 | 0.0023 | 0.0004 | 0.0034 | 0.0016 | 0.002 | 0.0013 | 0.0011 | 0.001 | 0.0027 | 0.0013 |
R1 | R2 | |||||||||
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G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 32.8208 | 27.9427 | 20.7347 | 33.6522 | 30.3954 | 31.2406 | 29.43 | 32.7347 | 37.8242 | 37.1141 |
Translational Efficiency | 1.0212 | 1.0755 | 1.3678 | 0.9523 | 1.0043 | 1.2961 | 1.3349 | 0.9391 | 1.0441 | 1.0334 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
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37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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