Standard name | |
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Human Ortholog | |
Description | Essential subunit of the Dam1 complex (aka DASH complex); complex couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; is transferred to the kinetochore prior to mitosis |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 2 |
Bud | 1 | 0 | 1 | 3 | 5 | 4 | – | 7 | 9 | 9 | 4 | 3 | 0 | 0 | 0 | 0 | 1 | 0 | 3 | 7 | 2 | 1 | 3 | 6 |
Bud Neck | 4 | 3 | 4 | 1 | 3 | 5 | – | 5 | 1 | 7 | 5 | 2 | 5 | 1 | 2 | 1 | 0 | 0 | 2 | 2 | 1 | 0 | 1 | 2 |
Bud Site | 3 | 3 | 11 | 6 | 2 | 3 | – | 4 | 9 | 15 | 11 | 16 | 1 | 2 | 1 | 1 | 0 | 1 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 5 | 7 | 2 | 9 | 11 | 9 | – | 12 | 9 | 8 | 15 | 8 | 15 | 36 | 53 | 9 | 12 | 7 | 0 | 0 | 0 | 0 | 0 | 5 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 3 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 1 |
Endosome | 1 | 9 | 2 | 2 | 17 | 17 | – | 5 | 6 | 2 | 3 | 2 | 13 | 13 | 31 | 10 | 11 | 6 | 4 | 7 | 6 | 10 | 15 | 23 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 1 |
Mitochondria | 17 | 11 | 12 | 13 | 8 | 14 | – | 11 | 19 | 17 | 18 | 23 | 4 | 6 | 6 | 4 | 8 | 3 | 2 | 1 | 3 | 1 | 5 | 16 |
Nucleus | 8 | 9 | 5 | 9 | 5 | 13 | – | 9 | 9 | 6 | 7 | 11 | 6 | 9 | 12 | 3 | 4 | 2 | 2 | 6 | 2 | 2 | 5 | 0 |
Nuclear Periphery | 0 | 1 | 0 | 1 | 0 | 1 | – | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 89 | 97 | 72 | 74 | 90 | 116 | – | 142 | 98 | 117 | 85 | 87 | 131 | 160 | 166 | 66 | 75 | 49 | 6 | 17 | 7 | 9 | 15 | 19 |
Peroxisomes | 2 | 11 | 4 | 27 | 51 | 40 | – | 50 | 41 | 52 | 20 | 22 | 14 | 33 | 35 | 13 | 3 | 3 | 0 | 0 | 0 | 6 | 8 | 7 |
SpindlePole | 81 | 136 | 106 | 110 | 145 | 141 | – | 161 | 128 | 163 | 77 | 108 | 165 | 212 | 191 | 51 | 56 | 41 | 111 | 158 | 128 | 112 | 217 | 174 |
Vac/Vac Membrane | 1 | 3 | 2 | 0 | 2 | 0 | – | 2 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 6 | 4 | 1 | 0 | 1 | 0 | 0 | 0 | 2 |
Unique Cell Count | 134 | 198 | 146 | 170 | 215 | 220 | 274 | 199 | 261 | 161 | 182 | 231 | 342 | 340 | 105 | 111 | 71 | 143 | 210 | 157 | 158 | 307 | 311 | |
Labelled Cell Count | 212 | 290 | 221 | 255 | 339 | 363 | 408 | 329 | 396 | 245 | 284 | 354 | 474 | 499 | 167 | 176 | 114 | 143 | 210 | 157 | 158 | 307 | 311 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 5.8 | 6.7 | 5.3 | 6.0 | 7.0 | 6.2 | – | 6.5 | 6.0 | 6.3 | 6.4 | 6.0 | 7.6 | 7.5 | 7.3 | 11.5 | 11.1 | 10.0 | 6.7 | 7.0 | 7.2 |
Std Deviation (1e-4) | 1.1 | 1.4 | 1.6 | 2.0 | 2.0 | 2.1 | – | 2.0 | 2.4 | 2.0 | 3.0 | 2.0 | 2.4 | 2.2 | 2.3 | 3.6 | 3.2 | 2.9 | 1.9 | 1.5 | 2.1 |
Intensity Change (Log2) | – | – | – | 0.17 | 0.4 | 0.21 | – | 0.28 | 0.16 | 0.24 | 0.25 | 0.18 | 0.5 | 0.49 | 0.46 | 1.1 | 1.06 | 0.9 | 0.33 | 0.4 | 0.43 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | -1.6 | -3.3 | -3.0 | – | -3.2 | -1.2 | -0.7 | -0.2 | 0.4 | 0 | 0 | 0 | -2.4 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 1.9 | 1.9 | 0 | – | 0 | 0 | 0 | 3.0 | 0 | 2.3 | 3.5 | 4.5 | 0 | 3.3 | 0 |
Endoplasmic Reticulum | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 2.7 | 2.7 | – | 0 | 0 | 0 | 0 | 0 | 2.1 | 0 | 3.1 | 3.0 | 3.1 | 0 |
Golgi | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | -0.2 | -1.8 | -0.7 | – | -1.8 | 0.4 | -0.6 | 0.9 | 1.3 | -3.0 | -3.5 | -3.5 | -1.4 | -0.3 | 0 |
Nucleus | 0.8 | 0 | 1.1 | – | 0 | 0.5 | 0 | 0.4 | 1.1 | 0 | 0 | 0.1 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | -1.0 | -1.4 | 0.6 | – | 0.5 | 0 | -0.9 | 0.6 | -0.3 | 1.4 | -0.5 | -0.1 | 2.1 | 2.9 | 2.7 |
Peroxisomes | 3.9 | 5.4 | 4.4 | – | 4.5 | 4.9 | 4.8 | 3.2 | 3.1 | 1.5 | 2.6 | 2.8 | 3.0 | 0 | 0 |
SpindlePole | -1.5 | -1.0 | -1.7 | – | -2.8 | -1.6 | -2.1 | -4.4 | -2.5 | -0.2 | -2.3 | -3.4 | -3.9 | -3.6 | -2.2 |
Vacuole | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 3.2068 | 3.4457 | 3.2759 | 2.7081 | 2.6145 | 3.1436 | 2.7984 | 2.3116 | 2.6988 | 2.5262 | 2.4823 | 2.215 | 4.9903 | 4.9858 | 4.8082 | 4.4429 | 4.8297 | 4.3489 |
Actin | 0.0226 | 0 | 0.0087 | 0.0056 | 0.0035 | 0.0018 | 0.0019 | 0 | 0.0014 | 0.0277 | 0.0002 | 0.0001 | 0.0035 | 0 | 0.0056 | 0.0015 | 0.0007 | 0.0001 |
Bud | 0.0004 | 0 | 0.0001 | 0 | 0.0002 | 0.0001 | 0.0001 | 0 | 0 | 0.0001 | 0 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0.0001 | 0 |
Bud Neck | 0.0014 | 0.0002 | 0.0002 | 0.0003 | 0.0006 | 0.0005 | 0.0014 | 0.0002 | 0.0003 | 0.0019 | 0.0002 | 0.0002 | 0.0061 | 0.0002 | 0.0009 | 0.0012 | 0.0002 | 0.0002 |
Bud Periphery | 0.0008 | 0 | 0.0001 | 0 | 0.0003 | 0.0002 | 0.0001 | 0 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0001 | 0 | 0.0002 | 0 |
Bud Site | 0.0075 | 0.0002 | 0.0003 | 0.0001 | 0.0007 | 0.0002 | 0.0041 | 0.0002 | 0.0005 | 0.0004 | 0.0001 | 0.0001 | 0.0034 | 0.0001 | 0.0015 | 0.0003 | 0.0006 | 0 |
Cell Periphery | 0.0004 | 0 | 0 | 0 | 0.0001 | 0 | 0.0002 | 0 | 0 | 0 | 0 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0.0001 | 0 |
Cytoplasm | 0.0017 | 0 | 0.0008 | 0.0001 | 0.0094 | 0.0001 | 0.002 | 0.0014 | 0.0003 | 0.0002 | 0.0001 | 0.0001 | 0.0008 | 0 | 0.0002 | 0.0004 | 0.0001 | 0 |
Cytoplasmic Foci | 0.0187 | 0.0063 | 0.0174 | 0.0262 | 0.0192 | 0.0057 | 0.0261 | 0.01 | 0.0205 | 0.0174 | 0.0171 | 0.0041 | 0.0208 | 0.0037 | 0.0056 | 0.015 | 0.0119 | 0.0046 |
Eisosomes | 0.0004 | 0 | 0 | 0 | 0 | 0.0001 | 0 | 0 | 0 | 0.0002 | 0 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.0024 | 0 | 0.0005 | 0.0001 | 0.0061 | 0.0002 | 0.0008 | 0.0004 | 0.0002 | 0.0003 | 0.0002 | 0 | 0.0031 | 0 | 0.0002 | 0.0001 | 0.0001 | 0 |
Endosome | 0.0132 | 0.0001 | 0.0133 | 0.0021 | 0.028 | 0.0054 | 0.014 | 0.0033 | 0.0076 | 0.0049 | 0.0215 | 0.0001 | 0.0055 | 0.0001 | 0.0012 | 0.0052 | 0.0133 | 0.0001 |
Golgi | 0.0049 | 0.0001 | 0.0038 | 0.0033 | 0.0123 | 0.0047 | 0.0029 | 0.0002 | 0.0088 | 0.01 | 0.0185 | 0.0002 | 0.0014 | 0.0001 | 0.0009 | 0.0028 | 0.0067 | 0.0002 |
Lipid Particles | 0.0335 | 0.004 | 0.0166 | 0.0187 | 0.0261 | 0.014 | 0.0198 | 0.0085 | 0.0287 | 0.0365 | 0.0588 | 0.0078 | 0.0136 | 0.0025 | 0.0023 | 0.0116 | 0.0382 | 0.0046 |
Mitochondria | 0.0118 | 0 | 0.0068 | 0.0003 | 0.0036 | 0.0091 | 0.0009 | 0.0001 | 0.0019 | 0.0051 | 0.0021 | 0.0047 | 0.0008 | 0 | 0.0007 | 0.0004 | 0.0136 | 0.0009 |
None | 0.0016 | 0 | 0.0006 | 0.0002 | 0.0024 | 0.0001 | 0.0026 | 0.0017 | 0.0001 | 0.0003 | 0.0001 | 0.0001 | 0.0013 | 0 | 0.0004 | 0.0008 | 0.0002 | 0 |
Nuclear Periphery | 0.0048 | 0.0002 | 0.0026 | 0.0011 | 0.0045 | 0.0033 | 0.0101 | 0.0045 | 0.0048 | 0.0009 | 0.0018 | 0.0008 | 0.0184 | 0.0002 | 0.0006 | 0.0011 | 0.0014 | 0.0011 |
Nucleolus | 0.1067 | 0.1254 | 0.0871 | 0.0222 | 0.0093 | 0.0562 | 0.0535 | 0.1396 | 0.0854 | 0.0078 | 0.0664 | 0.0644 | 0.077 | 0.147 | 0.0614 | 0.0126 | 0.0358 | 0.0578 |
Nucleus | 0.0108 | 0.0408 | 0.0077 | 0.002 | 0.0023 | 0.0068 | 0.0114 | 0.0344 | 0.0118 | 0.0012 | 0.0038 | 0.0092 | 0.0093 | 0.0326 | 0.0155 | 0.0017 | 0.0031 | 0.0089 |
Peroxisomes | 0.0153 | 0.0002 | 0.0052 | 0.0069 | 0.0074 | 0.0066 | 0.0033 | 0.0006 | 0.0064 | 0.0108 | 0.0016 | 0.0026 | 0.0064 | 0.0001 | 0.002 | 0.0239 | 0.0077 | 0.0008 |
Punctate Nuclear | 0.7378 | 0.8225 | 0.8264 | 0.9105 | 0.8621 | 0.8842 | 0.8424 | 0.793 | 0.8207 | 0.8735 | 0.8054 | 0.9054 | 0.8261 | 0.8134 | 0.9004 | 0.9213 | 0.8638 | 0.9206 |
Vacuole | 0.0024 | 0 | 0.0007 | 0.0001 | 0.0012 | 0.0004 | 0.0017 | 0.001 | 0.0002 | 0.0001 | 0.0008 | 0 | 0.0008 | 0 | 0.0002 | 0.0001 | 0.0011 | 0 |
Vacuole Periphery | 0.001 | 0 | 0.0007 | 0.0001 | 0.0007 | 0.0003 | 0.0007 | 0.0008 | 0.0003 | 0.0002 | 0.0011 | 0 | 0.0013 | 0 | 0.0001 | 0.0001 | 0.0009 | 0 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 26.0755 | 14.722 | 10.9244 | 14.1792 | 19.5731 | 43.8923 | 8.6832 | 10.0467 | 17.0439 | 16.1978 |
Translational Efficiency | 2.1078 | 1.8245 | 2.133 | 2.3284 | 2.1697 | 1.3537 | 2.6519 | 1.8849 | 1.7472 | 2.5969 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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