Standard name | |
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Human Ortholog | |
Description | Mitochondrial ribosomal protein of the large subunit |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | – | – | 0 | – | 0 | – | 0 | 0 | 0 | 0 | 1 | 2 | 1 | – | – | – | 6 | 1 | 0 | 2 | 4 | 4 |
Bud | 0 | 1 | – | – | 0 | – | 0 | – | 2 | 0 | 3 | 3 | 1 | 3 | 1 | – | – | – | 4 | 0 | 0 | 0 | 2 | 4 |
Bud Neck | 0 | 0 | – | – | 0 | – | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 | 0 | 1 | 2 |
Bud Site | 1 | 0 | – | – | 0 | – | 1 | – | 1 | 0 | 1 | 0 | 0 | 0 | 0 | – | – | – | – | – | – | – | – | – |
Cell Periphery | 16 | 1 | – | – | 0 | – | 0 | – | 1 | 0 | 0 | 2 | 8 | 7 | 9 | – | – | – | 0 | 0 | 0 | 0 | 1 | 0 |
Cytoplasm | 3 | 0 | – | – | 0 | – | 12 | – | 5 | 10 | 1 | 8 | 8 | 6 | 8 | – | – | – | 0 | 0 | 0 | 0 | 1 | 1 |
Endoplasmic Reticulum | 0 | 2 | – | – | 0 | – | 2 | – | 0 | 3 | 0 | 3 | 2 | 0 | 5 | – | – | – | 1 | 0 | 0 | 0 | 1 | 0 |
Endosome | 87 | 3 | – | – | 2 | – | 5 | – | 0 | 0 | 0 | 2 | 7 | 7 | 29 | – | – | – | 32 | 4 | 0 | 11 | 24 | 25 |
Golgi | 7 | 0 | – | – | 10 | – | 7 | – | 0 | 2 | 0 | 3 | 15 | 23 | 52 | – | – | – | 17 | 3 | 1 | 8 | 15 | 17 |
Mitochondria | 316 | 64 | – | – | 11 | – | 66 | – | 84 | 113 | 84 | 153 | 153 | 242 | 260 | – | – | – | 443 | 60 | 13 | 132 | 235 | 195 |
Nucleus | 6 | 0 | – | – | 1 | – | 5 | – | 1 | 7 | 0 | 3 | 1 | 0 | 0 | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 23 | 4 | – | – | 0 | – | 13 | – | 6 | 7 | 5 | 10 | 12 | 7 | 8 | – | – | – | 13 | 2 | 0 | 2 | 9 | 9 |
Nucleolus | 31 | 2 | – | – | 0 | – | 7 | – | 6 | 7 | 4 | 10 | 7 | 4 | 5 | – | – | – | 0 | 0 | 0 | 0 | 7 | 10 |
Peroxisomes | 40 | 0 | – | – | 0 | – | 2 | – | 0 | 1 | 1 | 1 | 12 | 9 | 24 | – | – | – | 17 | 0 | 1 | 10 | 32 | 20 |
SpindlePole | 17 | 0 | – | – | 2 | – | 0 | – | 0 | 4 | 1 | 3 | 4 | 8 | 4 | – | – | – | 8 | 0 | 0 | 2 | 4 | 3 |
Vac/Vac Membrane | 118 | 11 | – | – | 3 | – | 8 | – | 4 | 7 | 3 | 6 | 21 | 27 | 37 | – | – | – | 14 | 4 | 0 | 3 | 9 | 11 |
Unique Cell Count | 477 | 71 | 21 | 97 | 93 | 137 | 89 | 175 | 180 | 266 | 331 | 567 | 80 | 18 | 178 | 358 | 312 | |||||||
Labelled Cell Count | 665 | 88 | 29 | 128 | 110 | 161 | 103 | 207 | 252 | 345 | 443 | 567 | 80 | 18 | 178 | 358 | 312 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 7.3 | 6.2 | – | – | 8.2 | – | 6.7 | – | 6.1 | 6.7 | 5.6 | 6.2 | 7.6 | 6.7 | 7.5 | – | – | – | 6.8 | 7.2 | 7.5 |
Std Deviation (1e-4) | 1.6 | 1.0 | – | – | 1.7 | – | 1.7 | – | 1.4 | 1.9 | 1.1 | 1.4 | 1.6 | 1.6 | 1.5 | – | – | – | 1.8 | 1.8 | 1.6 |
Intensity Change (Log2) | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 4.5857 | 4.3916 | 3.8839 | 3.7503 | 3.37 | 4.1123 | 6.689 | 6.866 | 6.9253 | 5.6908 | 5.8544 | 6.998 | 7.3885 | 8.2777 | 7.8937 | 8.0821 | 6.9662 | 7.3821 |
Actin | 0.0219 | 0.0031 | 0.0048 | 0.0527 | 0.0062 | 0.0185 | 0.021 | 0.0083 | 0.0057 | 0.0009 | 0.0025 | 0.0045 | 0.015 | 0.0015 | 0.0059 | 0.0001 | 0.0055 | 0.0166 |
Bud | 0.0003 | 0.0007 | 0.0011 | 0.0009 | 0.0002 | 0.0001 | 0.0003 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0004 | 0.0028 | 0 | 0.0002 | 0.0001 |
Bud Neck | 0.0003 | 0.0003 | 0.0002 | 0.0006 | 0.0006 | 0.0003 | 0.0002 | 0.0001 | 0.0002 | 0 | 0 | 0 | 0.0001 | 0.0004 | 0.0004 | 0 | 0.0011 | 0.0001 |
Bud Periphery | 0.0005 | 0.0009 | 0.0021 | 0.0009 | 0.0002 | 0.0003 | 0.0007 | 0.0002 | 0.0004 | 0.0002 | 0.0002 | 0.0002 | 0.0004 | 0.0004 | 0.0029 | 0.0001 | 0.0004 | 0.0002 |
Bud Site | 0.001 | 0.0025 | 0.0023 | 0.0172 | 0.0006 | 0.0002 | 0.0003 | 0.0002 | 0.0019 | 0.0001 | 0.0001 | 0.0001 | 0.0006 | 0.0095 | 0.0097 | 0 | 0.0023 | 0.0001 |
Cell Periphery | 0.0002 | 0.0002 | 0.0003 | 0.0002 | 0.0001 | 0.0002 | 0.0003 | 0.0001 | 0.0004 | 0.0001 | 0.0001 | 0.0001 | 0.0002 | 0.0002 | 0.0003 | 0 | 0.0002 | 0.0001 |
Cytoplasm | 0.0017 | 0 | 0.0027 | 0.0003 | 0.0002 | 0.0003 | 0.0018 | 0.0001 | 0.0009 | 0 | 0.0006 | 0 | 0.0002 | 0 | 0.0002 | 0 | 0.0001 | 0.0001 |
Cytoplasmic Foci | 0.021 | 0.0036 | 0.0147 | 0.0063 | 0.0228 | 0.0037 | 0.0028 | 0.0022 | 0.0062 | 0.0003 | 0.0051 | 0.0011 | 0.0054 | 0.0037 | 0.0008 | 0.0001 | 0.0048 | 0.0013 |
Eisosomes | 0.0003 | 0.0002 | 0.0002 | 0.0004 | 0.0002 | 0.0002 | 0.0004 | 0.0002 | 0.0004 | 0.0001 | 0.0001 | 0.0001 | 0.0004 | 0.0002 | 0.0005 | 0 | 0.0002 | 0.0004 |
Endoplasmic Reticulum | 0.0018 | 0.0001 | 0.0086 | 0.0002 | 0.0001 | 0.0013 | 0.0014 | 0.0001 | 0.0009 | 0.0001 | 0.001 | 0.0002 | 0.0002 | 0.0001 | 0.0002 | 0 | 0.0001 | 0.0004 |
Endosome | 0.032 | 0.0105 | 0.0209 | 0.0522 | 0.0215 | 0.0086 | 0.014 | 0.0119 | 0.0168 | 0.0025 | 0.0332 | 0.0068 | 0.0114 | 0.0088 | 0.0011 | 0.001 | 0.0326 | 0.0044 |
Golgi | 0.037 | 0.0351 | 0.0075 | 0.0264 | 0.0789 | 0.0089 | 0.0157 | 0.0511 | 0.0196 | 0.0089 | 0.0104 | 0.0265 | 0.0187 | 0.041 | 0.002 | 0.0029 | 0.0503 | 0.0103 |
Lipid Particles | 0.0236 | 0.025 | 0.0039 | 0.0047 | 0.0037 | 0.012 | 0.0145 | 0.0066 | 0.0126 | 0.0007 | 0.0156 | 0.003 | 0.0126 | 0.0029 | 0.0015 | 0.0002 | 0.0265 | 0.0103 |
Mitochondria | 0.7846 | 0.8917 | 0.9134 | 0.7924 | 0.8133 | 0.9148 | 0.8747 | 0.9101 | 0.9051 | 0.984 | 0.9237 | 0.9518 | 0.9191 | 0.9103 | 0.9657 | 0.9948 | 0.8437 | 0.9421 |
None | 0.004 | 0.0001 | 0.0006 | 0.0002 | 0.0001 | 0.0003 | 0.0017 | 0 | 0.0023 | 0 | 0.0004 | 0.0001 | 0.0008 | 0 | 0.0003 | 0 | 0.0001 | 0.0006 |
Nuclear Periphery | 0.0041 | 0.0001 | 0.004 | 0.0001 | 0 | 0.0057 | 0.0134 | 0.0001 | 0.0031 | 0 | 0.0019 | 0.0005 | 0.0008 | 0 | 0.0001 | 0 | 0.0001 | 0.0021 |
Nucleolus | 0.0008 | 0.0003 | 0.0001 | 0.0001 | 0 | 0.0024 | 0.0002 | 0 | 0.0003 | 0 | 0 | 0 | 0.0009 | 0.0004 | 0.0002 | 0 | 0.0009 | 0.0001 |
Nucleus | 0.001 | 0.0001 | 0.0006 | 0.0001 | 0 | 0.0018 | 0.0027 | 0 | 0.0055 | 0 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0 | 0.0002 | 0.0001 |
Peroxisomes | 0.031 | 0.0167 | 0.0059 | 0.0421 | 0.049 | 0.0141 | 0.0081 | 0.0031 | 0.0055 | 0.0006 | 0.0025 | 0.0017 | 0.005 | 0.0169 | 0.0035 | 0.0002 | 0.0183 | 0.0049 |
Punctate Nuclear | 0.0057 | 0 | 0.0003 | 0.0003 | 0.0003 | 0.0011 | 0.0017 | 0 | 0.0013 | 0 | 0.0011 | 0.0003 | 0.0021 | 0 | 0 | 0 | 0.0001 | 0.0021 |
Vacuole | 0.0105 | 0.003 | 0.0031 | 0.0006 | 0.0005 | 0.0011 | 0.0059 | 0.0009 | 0.0032 | 0.0004 | 0.0004 | 0.0003 | 0.0021 | 0.0012 | 0.0006 | 0.0001 | 0.0036 | 0.0004 |
Vacuole Periphery | 0.0164 | 0.0059 | 0.0029 | 0.0014 | 0.0014 | 0.004 | 0.0182 | 0.0043 | 0.0077 | 0.001 | 0.0009 | 0.0024 | 0.0035 | 0.0018 | 0.0013 | 0.0004 | 0.0086 | 0.0035 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 49.7283 | 38.5827 | 43.5675 | 37.1742 | 34.0622 | 50.6385 | 41.1311 | 56.6663 | 40.933 | 49.5838 |
Translational Efficiency | 1.0526 | 1.2243 | 0.9776 | 1.2374 | 1.6398 | 1.5079 | 1.4471 | 1.156 | 1.5033 | 1.3973 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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