Standard name
Human Ortholog
Description DEAH-box RNA helicase involved in second catalytic step of splicing and in exon ligation; exhibits ATP-dependent RNA unwinding activity; mediates the release of Yju2p and Cwc25p in the second step; in the absence of ATP, stabilizes the binding of Cwc25p to the spliceosome in the first catalytic step; missense mutation in human ortholog DHX38 associated with early-onset retinitis pigmentosa

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.05 0.09 0.14 0.11 0.24 0.25 0.25 0.21 0.21 0.26 0.19 0.27 0.08 0.29 0.29 0.14 0.14 0.11 0 0 0.08 0.08 0.07 0.07
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.06 0.44 0.67 0.72 0.5 0.73 0.73 0.79 0.86 0.82 0.85 0.84 0.28 0.08 0.29 0.08 0.06 0.05 0 0 0 0 0 0
Nucleus 0.86 0.66 0.47 0.49 0.33 0.27 0.26 0.2 0.15 0.19 0.17 0.16 0.7 0.61 0.47 0.65 0.68 0.69 0.74 0.74 0.49 0.66 0.52 0.44
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.08 0.17 0 0 0.08 0 0.06 0 0 0 0.06 0.05 0.1 0.1 0.15 0.2 0.15 0.19 0.17 0.18 0.33 0.17 0.29 0.35
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0
Bud 0 0 1 0 0 2 1 3 1 3 1 2 0 0 0 0 0 1 1 0 1 1 3 5
Bud Neck 1 0 0 0 0 0 1 2 0 1 3 0 0 0 0 0 0 1 0 0 0 0 2 2
Bud Site 0 0 0 1 1 0 6 4 2 4 2 6 0 1 0 0 0 0
Cell Periphery 0 0 1 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0
Cytoplasm 9 13 18 14 31 51 60 63 41 61 21 63 9 42 52 29 29 28 3 4 11 9 16 18
Endoplasmic Reticulum 2 0 1 0 0 0 1 0 0 0 0 1 0 0 0 13 8 9 0 0 0 0 2 2
Endosome 1 0 0 0 2 0 0 0 0 0 0 0 0 0 0 7 5 7 2 0 1 2 1 2
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 3 1 3 3 0 0 0 1 0
Mitochondria 12 63 85 88 65 148 177 234 172 195 93 195 32 11 53 16 12 14 3 5 4 2 6 11
Nucleus 162 95 60 60 42 55 64 61 29 45 19 38 81 89 85 138 139 183 162 107 67 76 130 114
Nuclear Periphery 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 1
Nucleolus 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 2 3
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0
SpindlePole 0 0 0 0 1 0 0 1 1 1 0 3 0 0 0 1 2 1 2 0 1 1 4 5
Vac/Vac Membrane 16 25 3 3 10 8 14 8 5 4 7 11 11 14 28 42 30 49 37 25 45 19 73 90
Unique Cell Count 189 144 127 123 129 202 244 298 199 237 110 232 115 145 181 212 204 264 220 146 137 116 251 262
Labelled Cell Count 203 196 169 166 153 264 326 376 251 314 146 319 133 157 219 250 226 297 220 146 137 116 251 262


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.8 3.7 3.0 3.2 3.4 2.9 2.7 2.7 2.3 2.5 2.5 2.2 4.0 4.4 3.8 6.2 6.5 6.7 4.2 4.5 4.8
Std Deviation (1e-4) 0.7 0.5 0.2 0.8 1.3 0.7 1.0 0.4 0.3 0.9 0.7 0.7 0.7 1.2 1.0 1.8 2.0 1.9 0.9 1.4 1.4
Intensity Change (Log2) 0.08 0.19 -0.05 -0.14 -0.14 -0.37 -0.29 -0.25 -0.46 0.42 0.56 0.34 1.04 1.1 1.15 0.47 0.58 0.67


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin
Bud 0 0 0
Bud Neck 0 0 0
Bud Site 0 0 0
Cell Periphery 0 0 0
Cyto
Endoplasmic Reticulum 0 0 0
Endosome 0 0 0
Golgi 0 0 0
Mitochondria 0 0 0
Nuclear Periphery 0 0 0
Nuc
Nucleolus 0 0 0
Peroxisomes 0 0 0
SpindlePole 0 0 0
Vac
Cortical Patches 0 0 0
Cytoplasm 0 0 0
Nucleus 0 0 0
Vacuole 4.6 3.6 4.4

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.3284 0.8479 0.6806 0.5729 0.4958 0.7804 1.7854 1.4482 1.2661 0.8088 0.9687 1.1595 1.071 1.442 1.07 0.8986 0.8731 1.0577
Actin 0.1086 0.0004 0.0203 0.0006 0.0216 0.0084 0.0377 0.001 0.0071 0.027 0.0131 0.0079 0.0001 0.0001 0.0202 0.0002 0.0004 0.0001
Bud 0.0006 0.0001 0.0001 0.0001 0.0002 0.0002 0.0008 0.0001 0.0006 0.0001 0.0002 0.0002 0 0 0.0002 0.0013 0.0001 0
Bud Neck 0.0067 0.0008 0.0009 0.0013 0.0025 0.0058 0.0112 0.0003 0.0069 0.0023 0.0018 0.0021 0.0003 0.0002 0.0004 0.0022 0.0004 0.0011
Bud Periphery 0.0007 0.0001 0.0003 0.0003 0.0004 0.0003 0.0017 0.0001 0.0031 0.0001 0.0004 0.0013 0 0 0.0004 0.0022 0.0001 0
Bud Site 0.0105 0.0013 0.0005 0.0003 0.0012 0.0004 0.0054 0.0004 0.0023 0.0003 0.001 0.0002 0.0002 0.0002 0.0018 0.0115 0.0004 0
Cell Periphery 0.0003 0 0.0002 0.0002 0.0003 0 0.0009 0 0.0002 0 0.0001 0.0002 0.0001 0.0001 0.0003 0.0012 0 0
Cytoplasm 0.014 0.0402 0.0187 0.0079 0.0431 0.0222 0.0422 0.0418 0.0187 0.0207 0.0251 0.0133 0.0466 0.0678 0.0241 0.0036 0.0071 0.0179
Cytoplasmic Foci 0.0232 0.0015 0.0046 0.0012 0.0195 0.0022 0.0354 0.0006 0.0031 0.0013 0.0056 0.0011 0.0005 0.0009 0.0056 0.0031 0.0025 0.0005
Eisosomes 0.0007 0 0.0009 0.0002 0.0006 0 0.0003 0 0.0001 0 0.0003 0.0001 0.0001 0.0001 0.0001 0 0 0
Endoplasmic Reticulum 0.0046 0.0014 0.0018 0.0004 0.0115 0.0013 0.0148 0.0026 0.0017 0.0005 0.0051 0.0018 0.0037 0.0024 0.0019 0.0006 0.0003 0.0007
Endosome 0.0236 0.0011 0.0062 0.0022 0.0203 0.0076 0.0329 0.0041 0.0067 0.0012 0.0254 0.0051 0.0009 0.0014 0.0115 0.0202 0.006 0.0007
Golgi 0.0123 0.0001 0.0031 0.0012 0.007 0.0015 0.0125 0.0003 0.0022 0.0027 0.0027 0.0013 0 0 0.0029 0.002 0.0016 0
Lipid Particles 0.0274 0.0001 0.0047 0.0377 0.0262 0.0008 0.0339 0.0007 0.0013 0.0003 0.0116 0.0052 0.0001 0.0002 0.0064 0.0061 0.0233 0.0001
Mitochondria 0.0049 0.0004 0.0059 0.0544 0.0036 0.0012 0.0051 0.0006 0.0019 0.0007 0.005 0.0074 0.0003 0.0004 0.0033 0.0013 0.0018 0.0003
None 0.0253 0.0474 0.0242 0.0064 0.0486 0.0115 0.0431 0.0539 0.0421 0.0065 0.0065 0.0113 0.137 0.1947 0.0446 0.0009 0.1252 0.0648
Nuclear Periphery 0.0281 0.0162 0.017 0.0345 0.0456 0.0103 0.0253 0.0164 0.0233 0.0107 0.0446 0.0108 0.0132 0.0299 0.0171 0.0062 0.0044 0.0053
Nucleolus 0.0085 0.0045 0.0046 0.0105 0.0043 0.0102 0.0197 0.0032 0.0091 0.0026 0.0103 0.008 0.0063 0.0029 0.0049 0.0127 0.0055 0.0073
Nucleus 0.6539 0.8748 0.8689 0.8241 0.6515 0.8985 0.6249 0.8629 0.8489 0.9163 0.7883 0.9011 0.7801 0.6906 0.8232 0.8797 0.7892 0.887
Peroxisomes 0.0181 0.0003 0.0068 0.0024 0.0137 0.0004 0.0101 0.0001 0.0003 0.0006 0.0076 0.0032 0 0 0.0117 0.0008 0.0051 0
Punctate Nuclear 0.0252 0.0078 0.0075 0.0095 0.0743 0.016 0.0319 0.0072 0.017 0.0056 0.0323 0.0167 0.0085 0.0065 0.0162 0.0028 0.0258 0.0135
Vacuole 0.0016 0.0012 0.0019 0.0014 0.0022 0.0009 0.0085 0.0032 0.0025 0.0003 0.0091 0.001 0.0016 0.0012 0.0022 0.0384 0.0005 0.0004
Vacuole Periphery 0.0011 0.0003 0.0008 0.0034 0.0017 0.0004 0.0017 0.0005 0.0009 0.0001 0.0039 0.0006 0.0002 0.0003 0.0011 0.0032 0.0002 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 9.9499 10.4281 10.6968 12.2373 8.6744 8.2298 10.3113 10.1513 10.8163 14.5015
Translational Efficiency 0.6875 0.4537 0.4684 0.3662 0.5583 0.6564 0.4849 0.374 0.4405 0.3686

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency
Standard name
Human Ortholog
Description DEAH-box RNA helicase involved in second catalytic step of splicing and in exon ligation; exhibits ATP-dependent RNA unwinding activity; mediates the release of Yju2p and Cwc25p in the second step; in the absence of ATP, stabilizes the binding of Cwc25p to the spliceosome in the first catalytic step; missense mutation in human ortholog DHX38 associated with early-onset retinitis pigmentosa
Localization
Cell Percentages nucleus (50%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-3

Prp16

Prp16


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Prp16-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available