Standard name
Human Ortholog
Description Steryl ester hydrolase; one of three gene products (Yeh1p, Yeh2p, Tgl1p) responsible for steryl ester hydrolase activity and involved in sterol homeostasis; localized to lipid particle membranes; YEH1 has a paralog, YEH2, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0.12 0.06 0.18 0.16 0.25 0.18 0.09 0.1 0 0 0 0 0.17 0.21 0.3 0.35 0.31 0.39 0.21 0.18 0.27 0.25 0.22 0.15
Golgi 0 0 0.2 0 0.14 0 0 0 0 0 0 0 0 0 0 0.08 0.16 0.07 0.08 0.19 0.1 0.14 0.15 0.16
Mitochondria 0.64 0.77 0.36 0.48 0.41 0.71 0.7 0.62 0.78 0.7 0.82 0.79 0.28 0.16 0.11 0.14 0.2 0.14 0.06 0.14 0.05 0.12 0.08 0.06
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0.07 0.11 0.08 0 0 0 0.08 0.05 0.1 0.09 0.09 0.08 0 0 0 0.11 0.05 0.05 0 0 0 0 0 0
Nucleolus 0.21 0.13 0.12 0.14 0.06 0.09 0.13 0.19 0.22 0.22 0.25 0.23 0.24 0.21 0.33 0.3 0.18 0.28 0 0 0 0 0 0
Peroxisomes 0.41 0.21 0.25 0.45 0.19 0.15 0.11 0.09 0.05 0 0 0 0.49 0.42 0.43 0.16 0.16 0.28 0.54 0.35 0.49 0.35 0.4 0.49
SpindlePole 0.09 0.05 0.18 0.18 0.18 0.05 0.05 0.07 0 0 0 0 0.21 0.24 0.27 0.16 0.2 0.24 0.06 0 0 0 0 0.06
Vac/Vac Membrane 0 0 0.05 0 0 0 0 0.05 0 0.06 0 0 0 0 0 0.09 0.12 0.08 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 4 5 9 9 15 3 5 8 2 1 0 1 4 2 0 3 0 1 8 16 9 5 9 12
Bud 1 2 3 7 3 6 6 6 3 1 1 2 0 1 0 1 0 0 0 1 0 0 1 1
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 1 0 0 0 0 0 0 0
Bud Site 0 2 1 0 0 1 2 0 0 0 0 3 0 0 2 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 1 1 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0
Cytoplasm 0 4 2 4 12 10 13 13 5 6 1 3 4 8 11 10 12 6 0 0 0 0 4 0
Endoplasmic Reticulum 1 0 1 0 0 0 0 2 1 1 2 0 0 1 0 5 6 1 0 0 0 1 0 0
Endosome 47 16 42 43 63 69 43 42 15 13 4 12 42 54 105 92 76 107 87 50 86 38 54 46
Golgi 1 3 45 4 34 4 6 7 2 4 0 1 4 4 3 22 40 19 32 55 33 21 35 47
Mitochondria 246 198 82 130 101 271 324 268 284 230 261 270 72 43 39 36 50 37 25 40 16 17 19 18
Nucleus 1 1 0 2 3 2 7 7 2 3 4 5 0 0 4 3 2 1 0 0 0 0 0 0
Nuclear Periphery 26 29 19 8 7 14 38 20 35 30 29 28 8 9 13 30 13 15 2 4 1 1 0 4
Nucleolus 82 33 27 39 14 34 62 80 80 73 80 77 61 56 115 78 43 76 3 3 2 0 2 2
Peroxisomes 157 54 58 124 46 58 50 39 19 11 6 10 123 110 150 42 39 77 223 100 157 54 97 148
SpindlePole 34 12 41 49 44 19 21 30 11 13 7 6 53 62 92 43 50 66 26 10 9 6 5 17
Vac/Vac Membrane 0 4 12 0 3 12 11 22 10 18 8 15 3 2 8 23 29 21 0 2 1 1 6 1
Unique Cell Count 387 258 229 273 248 380 461 431 366 327 319 341 253 261 346 261 245 274 414 287 321 155 245 305
Labelled Cell Count 600 363 342 419 345 503 589 545 469 404 403 433 374 353 542 389 361 427 414 287 321 155 245 305


Lipid Particles

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 9.5 11.8 11.3 10.0 9.8 8.8 9.1 10.5 9.8 10.7 10.0 10.6 12.0 11.9 10.5 11.3 13.3 11.2 10.2 10.9 11.3
Std Deviation (1e-4) 2.2 3.0 2.0 2.1 1.9 2.0 2.0 2.5 2.7 2.8 2.8 2.7 2.5 2.6 2.1 2.9 11.6 3.5 2.3 2.3 2.7
Intensity Change (Log2) – – – -0.17 -0.19 -0.36 -0.3 -0.1 -0.2 -0.07 -0.17 -0.09 0.09 0.08 -0.1 0.01 0.24 -0.0 -0.14 -0.05 0.0


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches -0.4 1.1 0 0 -1.6 0 0 0 0 -1.6 -2.4 0 -2.0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 2.6 0 0 1.8 0 0 0 0 0 0 1.8 2.1 2.6 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome -0.8 1.9 -0.1 -3.4 -3.2 -5.7 -5.6 -7.1 -5.9 -0.5 0.7 3.2 4.2 3.2 5.1
Golgi -6.8 -1.7 -8.2 -8.7 -8.2 -8.4 -7.5 -8.3 -8.3 -6.6 -6.7 -8.0 -3.6 -0.9 -4.3
Mitochondria 2.7 1.1 8.6 8.7 6.5 10.2 8.1 11.0 10.4 -1.7 -4.9 -7.1 -5.7 -3.7 -5.9
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery -2.7 -2.6 -2.4 0 -1.9 0.5 0.4 0.3 0 -2.4 -2.3 -2.3 1.2 -1.3 -1.3
Nucleolus 0.8 -2.4 -1.1 0.6 2.2 3.1 3.2 3.9 3.3 3.5 2.8 5.8 4.9 1.8 4.4
Peroxisomes 4.7 -1.8 -3.1 -4.9 -5.6 -7.1 -7.7 -8.4 -8.1 5.3 3.9 4.4 -2.5 -2.5 0.7
SpindlePole 0 0 -5.2 -5.8 -4.3 -6.3 -5.5 -6.4 -6.9 0.8 1.6 2.4 -0.4 0.7 1.7
Vacuole -3.8 -2.5 -1.3 -2.0 -0.1 -1.6 0.1 -1.7 -0.5 -2.6 -3.0 -1.9 1.5 2.6 1.1

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 4.464 6.3976 5.8851 4.9949 4.698 5.7141 2.931 5.1286 4.946 4.2507 3.3249 4.5668 5.5625 8.638 7.6296 7.2868 6.6097 7.4444
Actin 0.0082 0.0009 0.0038 0.0197 0.0049 0.0005 0.0063 0.001 0.007 0.0109 0.0239 0.0048 0.0032 0.0004 0.0028 0.004 0.0014 0.0008
Bud 0.0002 0.0001 0.0001 0.0002 0.0009 0 0.0001 0.0003 0.0002 0.0004 0.0003 0.0001 0.0001 0 0.0001 0 0.0001 0
Bud Neck 0.0009 0.0005 0.0003 0.0019 0.0008 0.0003 0.0003 0.0002 0.0003 0.0002 0.0005 0.0003 0.0002 0.0003 0.0002 0.0001 0.0001 0.0001
Bud Periphery 0.0003 0.0004 0.0002 0.0002 0.0034 0.0001 0.0002 0.0004 0.0003 0.0004 0.0006 0.0001 0.0001 0 0.0002 0 0.0001 0.0001
Bud Site 0.0032 0.0009 0.0005 0.0007 0.0041 0.0001 0.0014 0.0022 0.0014 0.0008 0.0013 0.0003 0.0006 0.0001 0.0009 0.0001 0.0002 0.0001
Cell Periphery 0.0003 0.0004 0.0001 0.0001 0.0005 0.0002 0.0001 0.0004 0.0002 0.0001 0.0002 0.0001 0.0001 0.0001 0.0002 0 0.0001 0.0001
Cytoplasm 0.0008 0.0003 0.0005 0.0003 0.0009 0.0001 0.001 0.0001 0.0012 0.0005 0.001 0.0036 0.0004 0.0001 0.0001 0.0001 0.0001 0.0003
Cytoplasmic Foci 0.0603 0.1007 0.0688 0.0606 0.0562 0.0489 0.0595 0.0674 0.0549 0.0802 0.0461 0.0383 0.0733 0.063 0.067 0.0905 0.0652 0.042
Eisosomes 0.0001 0 0 0.0001 0.0003 0 0.0001 0 0.0002 0.0001 0.0003 0.0001 0 0 0.0002 0 0 0
Endoplasmic Reticulum 0.0013 0.0002 0.001 0.0005 0.0013 0.0003 0.0007 0.0001 0.0015 0.0002 0.0032 0.0005 0.0005 0.0001 0.0002 0.0001 0.0001 0.0013
Endosome 0.0549 0.0564 0.0354 0.0333 0.0805 0.024 0.0379 0.0254 0.0265 0.0176 0.0413 0.014 0.0207 0.0193 0.0129 0.0165 0.0273 0.0083
Golgi 0.0544 0.0609 0.0631 0.053 0.0659 0.0382 0.0331 0.0264 0.0368 0.0497 0.0585 0.0299 0.0485 0.0446 0.0397 0.0795 0.0517 0.0252
Lipid Particles 0.677 0.648 0.7214 0.6906 0.5837 0.7646 0.7506 0.7833 0.7608 0.7478 0.5921 0.8313 0.726 0.7892 0.7276 0.6527 0.6863 0.8436
Mitochondria 0.0112 0.0108 0.0228 0.0287 0.0552 0.0183 0.0104 0.0073 0.0236 0.0069 0.0441 0.019 0.0134 0.0069 0.0154 0.0063 0.0422 0.0073
None 0.0006 0.0001 0.0005 0.0002 0.0006 0.0001 0.0015 0.0001 0.0043 0.0003 0.0007 0.0003 0.0002 0 0.0001 0.0001 0.0001 0.0001
Nuclear Periphery 0.0044 0.0036 0.0039 0.002 0.0039 0.0073 0.01 0.0019 0.0032 0.0005 0.0036 0.0015 0.0007 0.0003 0.0037 0.0001 0.0007 0.0017
Nucleolus 0.0139 0.0278 0.002 0.0006 0.0184 0.0147 0.0103 0.0166 0.0215 0.0015 0.0032 0.0066 0.003 0.0045 0.0069 0.0001 0.0018 0.0022
Nucleus 0.0011 0.0046 0.0042 0.0002 0.0083 0.0016 0.001 0.0009 0.0012 0.0001 0.0006 0.0007 0.0002 0.0002 0.0003 0 0.0003 0.0003
Peroxisomes 0.0833 0.0603 0.0604 0.1022 0.0993 0.0597 0.05 0.0332 0.0443 0.0769 0.17 0.039 0.1005 0.0636 0.1169 0.1483 0.1047 0.061
Punctate Nuclear 0.0127 0.0038 0.0048 0.0026 0.0022 0.0027 0.0156 0.009 0.0046 0.0033 0.003 0.004 0.0046 0.0006 0.0017 0.0007 0.0104 0.001
Vacuole 0.0065 0.0113 0.0027 0.0008 0.0044 0.0098 0.002 0.0149 0.0025 0.0005 0.0023 0.0014 0.0013 0.0021 0.001 0.0002 0.0027 0.0014
Vacuole Periphery 0.0045 0.0079 0.0036 0.0015 0.0044 0.0084 0.008 0.0086 0.0035 0.0011 0.0032 0.0039 0.0024 0.0044 0.0017 0.0004 0.0043 0.0032

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 36.2035 33.2229 22.9526 16.2052 26.5851 28.1782 20.9948 18.7631 18.1601 24.4938
Translational Efficiency 0.7171 0.6037 0.5115 0.6603 0.5894 0.7299 0.643 0.3852 0.4701 0.5664

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘
Standard name
Human Ortholog
Description Steryl ester hydrolase; one of three gene products (Yeh1p, Yeh2p, Tgl1p) responsible for steryl ester hydrolase activity and involved in sterol homeostasis; localized to lipid particle membranes; YEH1 has a paralog, YEH2, that arose from the whole genome duplication
Localization
Cell Percentages —
Cell Cycle Regulation No
Subcompartmental Group N/A

Yeh1

Yeh1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Yeh1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available