Standard name
Human Ortholog
Description Protein of the mitochondrial outer surface; links the Arp2/3 complex with the mitochore during anterograde mitochondrial movement; also binds to and promotes degradation of mRNAs for select nuclear-encoded mitochondrial proteins
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0.12 0.08 0.1 0 0 0 0 0 0
Cytoplasm 0.99 0.94 0.92 0.96 0.93 0.83 0.78 0.73 0.67 0.56 0.57 0.57 0.99 0.99 0.98 0.89 0.9 0.9 0.93 0.91 0.86 0.74 0.75 0.75
Endoplasmic Reticulum 0 0 0 0 0 0 0.07 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0.14 0.14 0.1
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.13 0.17 0.11 0.38 0.45 0.52 0.31 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0.07 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0.05 0.05 0.1 0 0.07 0 0.1 0 0 0 0.09 0.06 0.05 0 0 0 0 0.05 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 5 2 2 2 10 8 6 3 4 5 0 0 0 1 5 2 0 1 0 0 1 2
Bud 0 0 1 2 0 2 0 5 6 9 12 2 3 1 1 0 0 0 0 1 0 0 0 4
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 1
Bud Site 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 5 1 15 6 9 10 4 17 7 9 8 5 7 14 9 49 28 35 0 0 0 0 0 1
Cytoplasm 178 167 257 205 214 224 251 282 188 169 153 122 335 429 439 357 325 302 174 164 260 212 330 278
Endoplasmic Reticulum 0 7 12 7 4 7 21 18 8 2 7 5 3 3 9 14 13 12 2 1 11 40 61 35
Endosome 1 0 2 0 1 3 1 13 2 2 0 5 0 0 0 2 3 0 1 1 0 1 2 2
Golgi 0 1 1 0 0 0 0 2 0 0 0 0 0 0 0 4 4 0 0 0 1 2 6 7
Mitochondria 0 4 1 0 4 35 54 41 106 136 141 67 1 1 1 2 3 6 0 0 11 9 9 12
Nucleus 1 0 2 1 1 0 3 3 2 6 2 14 2 3 0 5 2 0 2 1 1 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 1 0 0 2 0 0 0 0 0 0 0 0 0 0 1 1 0
Nucleolus 0 0 0 0 1 0 0 0 0 1 1 2 0 0 0 0 0 0 0 0 0 0 0 1
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 4 0 0 0 0 0 0 0 1 0 0 0 0 1 0 0 1
Vac/Vac Membrane 0 2 2 2 7 13 17 40 8 22 5 22 1 1 2 37 20 17 2 6 7 12 20 16
Unique Cell Count 179 177 279 213 231 270 322 387 280 304 269 214 340 432 447 399 360 334 188 180 301 286 443 370
Labelled Cell Count 185 182 298 225 243 296 361 434 333 360 335 249 352 452 461 472 403 374 188 180 301 286 443 370


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 9.8 9.6 9.3 8.5 8.7 7.3 6.7 7.4 6.2 6.1 5.5 6.2 9.8 10.1 10.0 12.5 12.1 12.5 9.0 9.1 9.7
Std Deviation (1e-4) 1.2 1.3 1.2 1.0 1.2 1.3 1.0 1.3 1.1 1.1 0.9 1.4 1.3 1.3 1.4 2.0 1.9 1.7 1.2 1.2 1.6
Intensity Change (Log2) – – – -0.14 -0.1 -0.36 -0.48 -0.33 -0.59 -0.6 -0.75 -0.6 0.07 0.12 0.1 0.42 0.38 0.42 -0.04 -0.04 0.06


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 1.0 0.3 0.3 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 3.2 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery -1.4 -0.8 -0.9 -2.9 -0.6 -1.7 -1.5 -1.4 -1.7 -2.2 -1.4 -2.5 3.0 1.2 2.3
Cytoplasm 1.9 0.2 -3.3 -4.8 -6.2 -7.3 -9.9 -9.5 -9.2 3.9 5.1 4.0 -1.2 -0.8 -0.7
Endoplasmic Reticulum -0.6 -1.7 -1.1 1.2 0.2 -0.9 -2.9 -1.1 -1.2 -2.8 -3.3 -1.8 -0.5 -0.4 -0.5
Endosome 0 0 0 0 2.3 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 6.0 7.0 5.4 11.3 12.6 13.9 9.9 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 3.6 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 2.9 3.2 5.0 0 4.0 0 4.9 0 0 0 4.7 3.3 3.1

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 6.887 8.2697 7.9679 7.6424 6.6707 7.8904 5.5703 7.2227 6.4282 6.2709 6.5374 6.4927 7.709 8.5596 8.2177 7.9423 7.9466 7.8915
Actin 0.037 0.0011 0.0093 0.0061 0.0107 0.0188 0.0247 0.0008 0.0125 0.0011 0.0006 0.0034 0.0393 0.0274 0.0355 0.024 0.0357 0.0408
Bud 0.001 0.0004 0.0014 0.001 0.0004 0.0006 0.0013 0.0004 0.008 0.0015 0.0011 0.0041 0.0247 0.0471 0.0622 0.0602 0.0394 0.0223
Bud Neck 0.0031 0.0004 0.0013 0.0012 0.001 0.0018 0.001 0.0003 0.001 0.0009 0.0009 0.0014 0.0062 0.0061 0.0091 0.0159 0.0079 0.0197
Bud Periphery 0.0015 0.0002 0.0018 0.0006 0.0003 0.0005 0.0014 0.0001 0.0026 0.0013 0.0002 0.002 0.0268 0.0267 0.0555 0.0621 0.0579 0.0244
Bud Site 0.0087 0.0028 0.0034 0.0015 0.0006 0.0005 0.0036 0.0011 0.0044 0.0005 0.0004 0.0004 0.0867 0.2501 0.1473 0.0283 0.0217 0.0097
Cell Periphery 0.0017 0.0007 0.0039 0.0003 0.0003 0.0004 0.0004 0.0002 0.0004 0.0002 0.0001 0.0002 0.0067 0.0035 0.0064 0.0068 0.0031 0.0017
Cytoplasm 0.59 0.9284 0.8605 0.8132 0.7407 0.8211 0.726 0.9493 0.8241 0.7945 0.8566 0.8478 0.5704 0.5427 0.4995 0.5971 0.6545 0.7534
Cytoplasmic Foci 0.0282 0.0108 0.0172 0.0248 0.0364 0.0207 0.0403 0.0113 0.0274 0.0309 0.028 0.0216 0.0329 0.015 0.0283 0.0268 0.0289 0.022
Eisosomes 0.0007 0 0.0017 0.0001 0.0001 0.0002 0.0002 0 0.0001 0 0 0.0001 0.0005 0.0002 0.0004 0.0002 0.0002 0.0001
Endoplasmic Reticulum 0.1287 0.0126 0.0182 0.0216 0.0101 0.0359 0.0311 0.0042 0.0128 0.0104 0.0054 0.019 0.0155 0.0024 0.0071 0.0082 0.0098 0.0068
Endosome 0.0643 0.0232 0.0403 0.0817 0.0622 0.0468 0.073 0.0212 0.0589 0.0933 0.0803 0.0581 0.0335 0.0047 0.0163 0.026 0.0239 0.0108
Golgi 0.0195 0.0029 0.0105 0.0046 0.0637 0.0172 0.0203 0.0045 0.0097 0.0165 0.0044 0.0176 0.0054 0.0012 0.0026 0.0048 0.0028 0.0018
Lipid Particles 0.0173 0.002 0.0019 0.0014 0.0025 0.0032 0.0152 0.0001 0.0011 0.0002 0 0.0027 0.0074 0.0005 0.0015 0.0022 0.0075 0.0022
Mitochondria 0.0114 0.001 0.0054 0.0024 0.0642 0.0171 0.0152 0.0009 0.0174 0.0246 0.0028 0.0111 0.013 0.0027 0.004 0.0026 0.0145 0.0018
None 0.0257 0.0033 0.0062 0.0055 0.0006 0.0012 0.0036 0.0006 0.0018 0.001 0.0008 0.0011 0.0597 0.0353 0.0541 0.076 0.0529 0.045
Nuclear Periphery 0.0195 0.0011 0.0023 0.004 0.0007 0.001 0.0061 0.0003 0.0035 0.0012 0.0009 0.0008 0.0076 0.0034 0.0062 0.0056 0.0045 0.0038
Nucleolus 0.0009 0 0.0002 0.0001 0 0 0.0005 0 0.0001 0 0 0 0.0038 0.0015 0.0035 0.0026 0.0014 0.002
Nucleus 0.006 0.0011 0.0012 0.0019 0.0006 0.0008 0.0035 0.0005 0.0024 0.0009 0.001 0.0008 0.0205 0.0156 0.028 0.017 0.0101 0.0154
Peroxisomes 0.0121 0.0001 0.0011 0.0005 0.0006 0.0064 0.0123 0.0001 0.0009 0.0005 0.0002 0.0017 0.0018 0.0012 0.0021 0.0009 0.0023 0.0015
Punctate Nuclear 0.0058 0.0003 0.0002 0.0011 0.0001 0.0005 0.0021 0 0.0006 0.0001 0.0001 0.0001 0.0054 0.0049 0.0065 0.0054 0.0034 0.0059
Vacuole 0.0125 0.0067 0.0096 0.021 0.003 0.0035 0.0143 0.0036 0.0079 0.0107 0.0126 0.0039 0.0293 0.0074 0.022 0.0253 0.0155 0.0077
Vacuole Periphery 0.0046 0.0007 0.0023 0.0055 0.0011 0.0017 0.0038 0.0007 0.0026 0.0099 0.0036 0.0021 0.0028 0.0006 0.0021 0.0019 0.002 0.0011

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 31.3467 29.1429 34.656 45.8106 30.8882 44.2788 52.0384 52.1418 45.3848 55.8627
Translational Efficiency 0.7868 0.8584 0.7906 0.4894 0.6479 0.9229 0.6993 0.7042 0.8933 0.7394

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Protein of the mitochondrial outer surface; links the Arp2/3 complex with the mitochore during anterograde mitochondrial movement; also binds to and promotes degradation of mRNAs for select nuclear-encoded mitochondrial proteins
Localization
Cell Percentages cytoplasm (99%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-5

Puf3

Puf3


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Puf3-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available