Standard name
Human Ortholog
Description Disaggregase; heat shock protein that cooperates with Ydj1p (Hsp40) and Ssa1p (Hsp70) to refold and reactivate previously denatured, aggregated proteins; responsive to stresses including: heat, ethanol, and sodium arsenite; involved in [PSI+] propagation; protein becomes more abundant and forms cytoplasmic foci in response to DNA replication stress; potentiated Hsp104p variants decrease TDP-43 proteotoxicity by eliminating its cytoplasmic aggregation
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.08
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0.05 0.06 – 0.06 0 0.07 0.09 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.96 0.95 0.93 0.85 0.87 0.82 0.83 – 0.79 0.8 0.77 0.76 0.8 0.82 0.92 0.84 0.85 0.79 0.91 0.88 0.92 0.77 0.57 0.53
Endoplasmic Reticulum 0 0 0 0 0.05 0.11 0.11 – 0.14 0.12 0.11 0.11 0.06 0.06 0 0 0 0.05 0 0 0 0.11 0.08 0.09
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0.1 0.1 0 0.07 0.06 0.05 0 0 0 0 0.06 0.06
Mitochondria 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0.09 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0.1 0 0 0 – 0 0 0 0 0 0 0 0.07 0.08 0.1 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 1 1 1 1 0 4 – 3 1 8 4 10 1 0 0 0 0 1 1 0 1 25 17
Bud 1 1 4 0 7 3 8 – 5 8 3 5 14 1 3 3 1 2 2 6 6 4 23 38
Bud Neck 0 1 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 2
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 1 1 10 8 12 19 25 – 19 11 23 30 2 5 1 1 1 1 0 0 0 0 0 2
Cytoplasm 347 485 480 248 379 293 374 – 241 254 248 263 359 384 486 305 215 144 338 505 497 340 257 256
Endoplasmic Reticulum 5 6 8 9 23 40 48 – 42 39 35 39 26 29 21 12 5 10 1 6 6 48 36 42
Endosome 0 2 0 0 0 0 1 – 0 0 0 0 0 0 0 0 0 1 3 2 2 3 20 17
Golgi 0 0 4 7 7 3 3 – 6 6 10 6 44 45 5 24 15 9 0 1 3 6 27 30
Mitochondria 0 1 4 3 6 2 0 – 1 1 2 0 2 2 5 6 3 4 0 0 0 3 4 11
Nucleus 0 0 1 0 0 1 0 – 1 0 1 1 1 0 0 0 0 0 3 14 3 3 1 1
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 2 1 3 1 4 5
Nucleolus 1 1 4 5 9 4 5 – 2 6 5 2 4 9 12 16 8 16 0 0 2 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 1 0 0 0 0 0 0 0 0 12 14
SpindlePole 0 1 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 2 0 3 0 13 17
Vac/Vac Membrane 11 20 22 28 14 5 2 – 5 8 8 13 14 5 6 26 20 19 5 24 9 17 12 13
Unique Cell Count 363 511 518 293 435 357 449 307 319 321 347 450 468 526 362 253 182 371 573 543 443 451 481
Labelled Cell Count 366 520 538 309 458 370 470 325 334 343 363 476 482 539 393 268 206 371 573 543 443 451 481


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 34.8 34.8 37.6 39.9 54.2 75.8 77.7 – 96.2 89.9 85.1 105.0 45.3 65.9 80.9 86.1 102.9 83.0 29.2 37.8 48.9
Std Deviation (1e-4) 10.2 10.4 16.7 24.0 24.4 38.6 29.1 – 46.9 43.3 36.8 55.2 18.6 24.8 28.5 55.6 64.5 55.5 11.4 16.9 21.5
Intensity Change (Log2) – – – 0.09 0.53 1.01 1.05 – 1.36 1.26 1.18 1.48 0.27 0.81 1.11 1.2 1.45 1.14 -0.36 0.01 0.38


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 – 0 0 0 0 3.0 0 0 0 0 0
Bud 0 1.2 0 1.4 – 0 0 0 0 2.7 0 0 0 0 0
Bud Neck 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0.7 0.8 2.8 3.0 – 3.2 1.4 3.8 4.6 -2.1 -1.1 -2.8 0 0 0
Cytoplasm -3.6 -2.9 -4.8 -4.5 – -5.9 -5.6 -6.4 -7.0 -5.9 -5.1 -0.2 -4.0 -3.4 -5.1
Endoplasmic Reticulum 1.5 3.2 6.2 6.1 – 7.1 6.5 6.0 6.2 3.6 3.8 2.4 1.7 0 0
Endosome 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Golgi 0 1.2 0 0 – 0 0 2.6 0 6.4 6.4 0 4.9 4.3 0
Mitochondria 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 1.7 0 0 – 0 0 0 0 0 1.6 2.0 3.6 0 5.6
Peroxisomes 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Vacuole 3.0 -0.8 -2.4 -3.8 – -2.0 -1.3 -1.3 -0.4 -0.9 -3.1 -3.1 1.9 2.1 3.1

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 29.4594 50.1393 42.6709 47.5835 39.8328 42.887 34.2338 48.8107 42.6155 43.9329 44.9896 49.3715 42.6135 57.8692 50.0723 51.1581 47.1787 51.0277
Actin 0.021 0.0001 0.0071 0.0005 0.0016 0.0011 0.0255 0.0002 0.0107 0.002 0.0001 0.0001 0.0005 0.0002 0.0002 0.0337 0.0002 0.0002
Bud 0.001 0.0001 0.0003 0.0003 0.0002 0.0002 0.0024 0.0022 0.0005 0.0003 0 0.0001 0.0004 0.0001 0.0001 0.0002 0.0001 0.0001
Bud Neck 0.0006 0.0002 0.0007 0.0004 0.0003 0.0007 0.001 0.0001 0.0005 0.0004 0.0005 0.0006 0.0002 0.0002 0.0002 0.0006 0.0004 0.0005
Bud Periphery 0.0013 0 0.0002 0 0.0001 0.0001 0.0032 0.0003 0.0002 0.0001 0 0 0.0003 0 0 0.0001 0 0
Bud Site 0.0019 0.0001 0.0007 0.0002 0.0002 0 0.002 0.0004 0.0011 0.0001 0 0 0.0003 0.0001 0 0.0001 0 0
Cell Periphery 0.0004 0 0.0001 0.0002 0.0004 0 0.0008 0.0001 0.0002 0 0 0 0.0003 0.0001 0 0 0 0.0001
Cytoplasm 0.7495 0.9815 0.9339 0.9437 0.9276 0.9178 0.8211 0.9635 0.9067 0.8636 0.9081 0.975 0.9138 0.9757 0.9832 0.9312 0.9871 0.9653
Cytoplasmic Foci 0.0233 0.002 0.0049 0.0009 0.0208 0.0013 0.017 0.0082 0.012 0.0032 0.0555 0.0008 0.0052 0.0029 0.001 0.0017 0.0005 0.0008
Eisosomes 0.0003 0 0.0001 0 0.0003 0 0.0003 0 0.0001 0 0 0 0.0001 0 0 0.0004 0 0
Endoplasmic Reticulum 0.0117 0.0003 0.0007 0.0004 0.01 0.0029 0.0084 0.0018 0.0055 0.0005 0.0007 0.0034 0.0037 0.0017 0.0004 0.0012 0.0006 0.0023
Endosome 0.0204 0.0003 0.0016 0.0005 0.0019 0.0051 0.0122 0.0004 0.0057 0.0056 0.0002 0.0008 0.0036 0.0007 0.0004 0.0028 0.0003 0.0005
Golgi 0.0054 0 0.0013 0.0001 0.0005 0.0006 0.0039 0 0.0018 0.0177 0 0 0.0004 0 0 0.0022 0 0
Lipid Particles 0.0244 0.0001 0.0109 0 0.0036 0.0009 0.0059 0 0.0034 0.0006 0.0001 0 0.0011 0.0003 0 0.0031 0 0
Mitochondria 0.0124 0.0001 0.0058 0.0001 0.0016 0.0005 0.0134 0.0002 0.0017 0.0071 0 0.0001 0.0037 0 0.0001 0.0006 0 0.0001
None 0.0118 0.0004 0.0008 0.0002 0.0147 0.0004 0.0027 0.0005 0.0011 0.0002 0.0001 0.0002 0.0077 0.0002 0.0005 0.0032 0.0002 0.0005
Nuclear Periphery 0.0334 0.0016 0.0053 0.004 0.006 0.0245 0.0153 0.002 0.0089 0.007 0.0052 0.0035 0.0101 0.0019 0.0017 0.0036 0.0016 0.0055
Nucleolus 0.0015 0.0012 0.0012 0 0.0004 0.0002 0.0019 0.0001 0.0003 0.0001 0 0 0.0029 0.0001 0.0001 0.0001 0 0.0008
Nucleus 0.0471 0.0097 0.0184 0.0437 0.0049 0.0364 0.0367 0.0178 0.0313 0.0874 0.0281 0.0138 0.0383 0.0148 0.0109 0.0101 0.0076 0.021
Peroxisomes 0.0028 0 0.0023 0 0.0002 0.0002 0.0067 0 0.0018 0.002 0 0 0.0002 0 0 0.0022 0 0
Punctate Nuclear 0.013 0.0012 0.0012 0.0001 0.0017 0.0022 0.0048 0.0004 0.0018 0.0006 0.0007 0.0001 0.0032 0.0002 0.0002 0.0017 0.0001 0.0008
Vacuole 0.0119 0.0007 0.0016 0.004 0.0021 0.0032 0.0094 0.0014 0.0033 0.0012 0.0005 0.0012 0.0029 0.0006 0.0006 0.0008 0.0011 0.0012
Vacuole Periphery 0.0048 0.0002 0.0007 0.0008 0.001 0.0014 0.0055 0.0002 0.0014 0.0004 0.0001 0.0003 0.0012 0.0001 0.0001 0.0004 0.0002 0.0003

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 101.3578 115.3847 130.9804 226.5621 126.9557 138.3041 185.6039 262.8822 294.867 160.456
Translational Efficiency 1.9003 2.1958 1.9935 1.8803 2.0131 2.0954 1.747 1.5778 1.8426 1.7492

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘
Standard name
Human Ortholog
Description Disaggregase; heat shock protein that cooperates with Ydj1p (Hsp40) and Ssa1p (Hsp70) to refold and reactivate previously denatured, aggregated proteins; responsive to stresses including: heat, ethanol, and sodium arsenite; involved in [PSI+] propagation; protein becomes more abundant and forms cytoplasmic foci in response to DNA replication stress; potentiated Hsp104p variants decrease TDP-43 proteotoxicity by eliminating its cytoplasmic aggregation
Localization
Cell Percentages cytoplasm (94%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-7

Hsp104

Hsp104


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Hsp104-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available