Standard name | |
---|---|
Human Ortholog | |
Description | Polyamine transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; recognizes spermine, putrescine, and spermidine; catalyzes uptake of polyamines at alkaline pH and excretion at acidic pH; during oxidative stress exports spermine, spermidine from the cell, which controls timing of expression of stress-responsive genes; phosphorylation enhances activity and sorting to the plasma membrane |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 30 | 37 | 80 | 43 | 31 | 58 | 62 | 93 | 46 | 78 | 52 | 78 | 96 | 99 | 53 | 42 | 21 | 28 | 5 | 8 | 11 | 12 | 8 | 16 |
Bud | 4 | 6 | 4 | 2 | 1 | 10 | 1 | 16 | 4 | 9 | 3 | 3 | 3 | 3 | 4 | 12 | 6 | 4 | 4 | 4 | 1 | 2 | 4 | 14 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 4 | 1 | 3 | 3 | 7 |
Bud Site | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 88 | 104 | 177 | 103 | 99 | 83 | 109 | 136 | 68 | 117 | 80 | 125 | 466 | 466 | 454 | 181 | 64 | 143 | 111 | 120 | 213 | 211 | 214 | 270 |
Cytoplasm | 11 | 16 | 16 | 12 | 14 | 40 | 25 | 34 | 20 | 25 | 18 | 20 | 40 | 40 | 25 | 20 | 10 | 11 | 4 | 1 | 0 | 2 | 3 | 1 |
Endoplasmic Reticulum | 11 | 10 | 19 | 52 | 59 | 67 | 126 | 140 | 46 | 90 | 51 | 61 | 16 | 21 | 25 | 4 | 3 | 9 | 8 | 7 | 16 | 10 | 4 | 9 |
Endosome | 0 | 1 | 0 | 0 | 0 | 2 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 3 | 2 | 1 | 1 | 4 | 1 | 2 | 0 | 4 | 0 | 0 | 3 | 0 | 0 | 3 | 0 | 0 | 0 | 0 | 0 | 1 |
Mitochondria | 4 | 8 | 40 | 20 | 3 | 50 | 31 | 34 | 39 | 53 | 46 | 42 | 1 | 1 | 0 | 8 | 7 | 21 | 1 | 1 | 3 | 9 | 7 | 7 |
Nucleus | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 1 | 1 | 1 | 1 | 2 | 2 | 1 | 0 | 0 | 4 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 2 | 7 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 3 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 2 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 0 | 0 | 1 | 0 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 0 | 0 | 2 | 0 | 0 | 0 | 3 | 2 | 2 |
Unique Cell Count | 115 | 129 | 226 | 157 | 155 | 210 | 249 | 312 | 155 | 241 | 160 | 209 | 512 | 517 | 511 | 215 | 83 | 165 | 139 | 151 | 249 | 258 | 254 | 334 |
Labelled Cell Count | 152 | 184 | 348 | 236 | 212 | 314 | 359 | 460 | 226 | 374 | 251 | 337 | 625 | 634 | 569 | 267 | 111 | 221 | 139 | 151 | 249 | 258 | 254 | 334 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 21.0 | 15.2 | 16.6 | 20.3 | 20.1 | 17.5 | 20.3 | 20.8 | 20.8 | 23.2 | 22.8 | 25.4 | 24.0 | 24.0 | 26.1 | 16.7 | 21.1 | 24.6 | 18.3 | 16.1 | 16.3 |
Std Deviation (1e-4) | 7.3 | 6.8 | 6.0 | 6.4 | 6.0 | 5.9 | 5.2 | 5.6 | 6.9 | 5.7 | 6.4 | 6.9 | 6.3 | 6.3 | 6.8 | 4.7 | 6.4 | 7.7 | 7.6 | 5.5 | 5.7 |
Intensity Change (Log2) | – | – | – | 0.28 | 0.27 | 0.07 | 0.28 | 0.32 | 0.32 | 0.48 | 0.46 | 0.61 | 0.53 | 0.53 | 0.65 | 0.01 | 0.34 | 0.56 | 0.14 | -0.04 | -0.03 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | -1.7 | -3.2 | -1.7 | -2.5 | -1.4 | -1.2 | -0.7 | -0.6 | 0.4 | -4.9 | -4.8 | -8.1 | -3.7 | -1.7 | -4.0 |
Bud | 0 | 0 | 1.8 | 0 | 2.0 | 0 | 1.3 | 0 | 0 | 0 | 0 | 0 | 2.1 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | -2.8 | -3.1 | -8.2 | -7.7 | -8.1 | -6.9 | -6.7 | -5.8 | -4.2 | 4.7 | 4.3 | 3.8 | 1.6 | -0.2 | 2.1 |
Cytoplasm | 0.2 | 0.7 | 3.7 | 1.1 | 1.5 | 1.9 | 1.3 | 1.4 | 0.9 | 0.3 | 0.3 | -1.2 | 0.9 | 1.4 | -0.2 |
Endoplasmic Reticulum | 6.1 | 7.0 | 6.2 | 10.0 | 9.1 | 5.4 | 7.4 | 5.9 | 5.6 | -3.1 | -2.4 | -1.9 | -3.1 | -1.5 | -1.1 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | -1.3 | -4.8 | 1.6 | -1.6 | -2.3 | 1.8 | 1.2 | 2.6 | 0.6 | -9.6 | -9.6 | -9.8 | -4.7 | -2.0 | -1.3 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 26.6095 | 41.0922 | 37.3847 | 32.5871 | 30.9688 | 37.0333 | 25.3349 | 35.7789 | 31.3581 | 28.2075 | 31.7626 | 33.4894 | 23.0471 | 33.2447 | 27.7067 | 25.8191 | 19.2526 | 28.289 |
Actin | 0.003 | 0.0004 | 0.0037 | 0.0068 | 0.0041 | 0.0007 | 0.0062 | 0.0002 | 0.0098 | 0.0006 | 0.0209 | 0.009 | 0.0304 | 0 | 0.0122 | 0.0015 | 0.0729 | 0.0001 |
Bud | 0.0011 | 0.0001 | 0.0007 | 0.0008 | 0.0003 | 0.0001 | 0.0017 | 0.0001 | 0.0012 | 0.0049 | 0.0001 | 0.0005 | 0.0033 | 0.0001 | 0.0002 | 0.0001 | 0.0007 | 0.0001 |
Bud Neck | 0.0003 | 0.0003 | 0.0009 | 0.0073 | 0.0035 | 0.0005 | 0.0006 | 0.0003 | 0.0018 | 0.0025 | 0.0011 | 0.0009 | 0.0018 | 0.0005 | 0.002 | 0.0009 | 0.0008 | 0.0017 |
Bud Periphery | 0.0146 | 0.0004 | 0.0047 | 0.0037 | 0.0015 | 0.0015 | 0.0116 | 0.0009 | 0.0094 | 0.0128 | 0.0019 | 0.0079 | 0.0166 | 0.0006 | 0.0018 | 0.003 | 0.0072 | 0.0024 |
Bud Site | 0.0018 | 0.0076 | 0.0022 | 0.002 | 0.0012 | 0.0001 | 0.0048 | 0.0026 | 0.0217 | 0.0098 | 0.0005 | 0.0003 | 0.0111 | 0.0139 | 0.0026 | 0.0005 | 0.0037 | 0.0001 |
Cell Periphery | 0.4536 | 0.5053 | 0.5182 | 0.3275 | 0.3832 | 0.3868 | 0.4195 | 0.5222 | 0.5173 | 0.451 | 0.4614 | 0.3788 | 0.3843 | 0.4488 | 0.5828 | 0.4752 | 0.3373 | 0.4055 |
Cytoplasm | 0.0259 | 0.0363 | 0.0129 | 0.0161 | 0.0279 | 0.0067 | 0.0032 | 0.0142 | 0.0078 | 0.0078 | 0.0082 | 0.005 | 0.0078 | 0.0127 | 0.0236 | 0.0075 | 0.0114 | 0.0035 |
Cytoplasmic Foci | 0.0055 | 0.0001 | 0.0009 | 0.0052 | 0.006 | 0.0017 | 0.0007 | 0.0001 | 0.0025 | 0.0012 | 0.0063 | 0.0014 | 0.0125 | 0 | 0.0041 | 0 | 0.0117 | 0 |
Eisosomes | 0.0166 | 0.0028 | 0.0045 | 0.0006 | 0.0008 | 0.0014 | 0.004 | 0.0015 | 0.0038 | 0.003 | 0.0009 | 0.001 | 0.0114 | 0.0008 | 0.0091 | 0.0027 | 0.0074 | 0.0013 |
Endoplasmic Reticulum | 0.3628 | 0.4005 | 0.3743 | 0.5318 | 0.4397 | 0.5628 | 0.3757 | 0.4145 | 0.3637 | 0.4401 | 0.4253 | 0.5576 | 0.4192 | 0.4861 | 0.3035 | 0.424 | 0.398 | 0.5669 |
Endosome | 0.0045 | 0 | 0.0019 | 0.0077 | 0.0145 | 0.0023 | 0.0033 | 0.0001 | 0.0008 | 0.0003 | 0.018 | 0.0018 | 0.0013 | 0 | 0.0005 | 0.0001 | 0.0486 | 0 |
Golgi | 0.0011 | 0.0001 | 0.0009 | 0.0014 | 0.0025 | 0.0005 | 0.0011 | 0.0002 | 0.001 | 0.0004 | 0.0077 | 0.0013 | 0.0043 | 0.0001 | 0.0016 | 0.0002 | 0.0265 | 0.0002 |
Lipid Particles | 0.0042 | 0.0053 | 0.004 | 0.0056 | 0.0138 | 0.0029 | 0.0037 | 0.0057 | 0.003 | 0.0031 | 0.0122 | 0.0025 | 0.022 | 0.0015 | 0.0068 | 0.0011 | 0.0323 | 0.0016 |
Mitochondria | 0.0109 | 0 | 0.0047 | 0.0068 | 0.0019 | 0.0005 | 0.0643 | 0 | 0.0024 | 0.0041 | 0.0013 | 0.0089 | 0.0012 | 0 | 0.0041 | 0.0589 | 0.0178 | 0 |
None | 0.0072 | 0.0001 | 0.0041 | 0.0092 | 0.0051 | 0.0005 | 0.0019 | 0.0001 | 0.0073 | 0.0021 | 0.0039 | 0.0004 | 0.0007 | 0 | 0.0002 | 0.0001 | 0.0029 | 0.0001 |
Nuclear Periphery | 0.0339 | 0.0231 | 0.0363 | 0.0304 | 0.0151 | 0.0125 | 0.0488 | 0.023 | 0.0253 | 0.0197 | 0.0164 | 0.0111 | 0.0295 | 0.0239 | 0.0236 | 0.0129 | 0.0091 | 0.0095 |
Nucleolus | 0.0031 | 0 | 0.0014 | 0.0032 | 0.0027 | 0.0001 | 0.0021 | 0 | 0.0004 | 0.0104 | 0.0001 | 0.0001 | 0.0002 | 0 | 0.0001 | 0 | 0.0004 | 0 |
Nucleus | 0.0105 | 0.0002 | 0.0013 | 0.01 | 0.0057 | 0.0004 | 0.0149 | 0.0002 | 0.0006 | 0.002 | 0.0004 | 0.0002 | 0.0005 | 0.0002 | 0.0003 | 0.0001 | 0.0008 | 0.0001 |
Peroxisomes | 0.0002 | 0 | 0.0003 | 0.0008 | 0.0065 | 0.0002 | 0.0006 | 0 | 0.0014 | 0.0007 | 0.0033 | 0.0009 | 0.0243 | 0 | 0.0089 | 0 | 0.0025 | 0 |
Punctate Nuclear | 0.0037 | 0 | 0.0011 | 0.0077 | 0.0385 | 0.0047 | 0.0044 | 0 | 0.0003 | 0.0001 | 0.0023 | 0.0016 | 0.0005 | 0 | 0.0001 | 0 | 0.001 | 0 |
Vacuole | 0.0327 | 0.017 | 0.0196 | 0.0131 | 0.0247 | 0.0128 | 0.0236 | 0.0138 | 0.0181 | 0.0211 | 0.0073 | 0.0086 | 0.0167 | 0.0105 | 0.0116 | 0.0108 | 0.0056 | 0.0069 |
Vacuole Periphery | 0.0025 | 0.0001 | 0.0015 | 0.0024 | 0.0007 | 0.0003 | 0.0032 | 0.0001 | 0.0006 | 0.0024 | 0.0005 | 0.0003 | 0.0004 | 0.0001 | 0.0002 | 0.0002 | 0.0013 | 0.0001 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 10.0253 | 15.6834 | 25.3538 | 28.2589 | 16.6539 | 11.2722 | 12.3179 | 12.4228 | 17.5583 | 11.3927 |
Translational Efficiency | 0.9387 | 1.2787 | 1.2822 | 1.2024 | 0.7625 | 1.0605 | 0.8599 | 1.1064 | 1.0391 | 0.6184 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✔ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
---|
Gene | Images |
---|