Standard name
Human Ortholog
Description Polyamine transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; recognizes spermine, putrescine, and spermidine; catalyzes uptake of polyamines at alkaline pH and excretion at acidic pH; during oxidative stress exports spermine, spermidine from the cell, which controls timing of expression of stress-responsive genes; phosphorylation enhances activity and sorting to the plasma membrane

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.26 0.29 0.35 0.27 0.2 0.28 0.25 0.3 0.3 0.32 0.33 0.37 0.19 0.19 0.1 0.2 0.25 0.17 0 0.06 0.05 0.05 0 0.05
Bud 0 0.05 0 0 0 0.05 0 0.05 0 0 0 0 0 0 0 0.06 0.07 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0.77 0.81 0.78 0.66 0.64 0.4 0.44 0.44 0.44 0.49 0.5 0.6 0.91 0.9 0.89 0.84 0.77 0.87 0.8 0.8 0.86 0.82 0.84 0.81
Cytoplasm 0.1 0.12 0.07 0.08 0.09 0.19 0.1 0.11 0.13 0.1 0.11 0.1 0.08 0.08 0.05 0.09 0.12 0.07 0 0 0 0 0 0
Endoplasmic Reticulum 0.1 0.08 0.08 0.33 0.38 0.32 0.51 0.45 0.3 0.37 0.32 0.29 0 0 0.05 0 0 0.05 0.06 0.05 0.06 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0.06 0.18 0.13 0 0.24 0.12 0.11 0.25 0.22 0.29 0.2 0 0 0 0 0.08 0.13 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 30 37 80 43 31 58 62 93 46 78 52 78 96 99 53 42 21 28 5 8 11 12 8 16
Bud 4 6 4 2 1 10 1 16 4 9 3 3 3 3 4 12 6 4 4 4 1 2 4 14
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 4 1 3 3 7
Bud Site 1 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 88 104 177 103 99 83 109 136 68 117 80 125 466 466 454 181 64 143 111 120 213 211 214 270
Cytoplasm 11 16 16 12 14 40 25 34 20 25 18 20 40 40 25 20 10 11 4 1 0 2 3 1
Endoplasmic Reticulum 11 10 19 52 59 67 126 140 46 90 51 61 16 21 25 4 3 9 8 7 16 10 4 9
Endosome 0 1 0 0 0 2 0 1 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 3 2 1 1 4 1 2 0 4 0 0 3 0 0 3 0 0 0 0 0 1
Mitochondria 4 8 40 20 3 50 31 34 39 53 46 42 1 1 0 8 7 21 1 1 3 9 7 7
Nucleus 0 0 1 0 1 0 0 0 1 0 1 0 0 0 1 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 1 1 1 1 2 2 1 0 0 4 0 1 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 2 7 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 3 0 1 0 0 1 0 0 0 0 0 0 2 2 2 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 1 0 1 1 1 0 0 0 0 0 1 1 1 0 0 2 0 0 0 3 2 2
Unique Cell Count 115 129 226 157 155 210 249 312 155 241 160 209 512 517 511 215 83 165 139 151 249 258 254 334
Labelled Cell Count 152 184 348 236 212 314 359 460 226 374 251 337 625 634 569 267 111 221 139 151 249 258 254 334


Bud, Cell Periphery, Endoplasmic Reticulum

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 21.0 15.2 16.6 20.3 20.1 17.5 20.3 20.8 20.8 23.2 22.8 25.4 24.0 24.0 26.1 16.7 21.1 24.6 18.3 16.1 16.3
Std Deviation (1e-4) 7.3 6.8 6.0 6.4 6.0 5.9 5.2 5.6 6.9 5.7 6.4 6.9 6.3 6.3 6.8 4.7 6.4 7.7 7.6 5.5 5.7
Intensity Change (Log2) 0.28 0.27 0.07 0.28 0.32 0.32 0.48 0.46 0.61 0.53 0.53 0.65 0.01 0.34 0.56 0.14 -0.04 -0.03


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches -1.7 -3.2 -1.7 -2.5 -1.4 -1.2 -0.7 -0.6 0.4 -4.9 -4.8 -8.1 -3.7 -1.7 -4.0
Bud 0 0 1.8 0 2.0 0 1.3 0 0 0 0 0 2.1 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery -2.8 -3.1 -8.2 -7.7 -8.1 -6.9 -6.7 -5.8 -4.2 4.7 4.3 3.8 1.6 -0.2 2.1
Cytoplasm 0.2 0.7 3.7 1.1 1.5 1.9 1.3 1.4 0.9 0.3 0.3 -1.2 0.9 1.4 -0.2
Endoplasmic Reticulum 6.1 7.0 6.2 10.0 9.1 5.4 7.4 5.9 5.6 -3.1 -2.4 -1.9 -3.1 -1.5 -1.1
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria -1.3 -4.8 1.6 -1.6 -2.3 1.8 1.2 2.6 0.6 -9.6 -9.6 -9.8 -4.7 -2.0 -1.3
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 26.6095 41.0922 37.3847 32.5871 30.9688 37.0333 25.3349 35.7789 31.3581 28.2075 31.7626 33.4894 23.0471 33.2447 27.7067 25.8191 19.2526 28.289
Actin 0.003 0.0004 0.0037 0.0068 0.0041 0.0007 0.0062 0.0002 0.0098 0.0006 0.0209 0.009 0.0304 0 0.0122 0.0015 0.0729 0.0001
Bud 0.0011 0.0001 0.0007 0.0008 0.0003 0.0001 0.0017 0.0001 0.0012 0.0049 0.0001 0.0005 0.0033 0.0001 0.0002 0.0001 0.0007 0.0001
Bud Neck 0.0003 0.0003 0.0009 0.0073 0.0035 0.0005 0.0006 0.0003 0.0018 0.0025 0.0011 0.0009 0.0018 0.0005 0.002 0.0009 0.0008 0.0017
Bud Periphery 0.0146 0.0004 0.0047 0.0037 0.0015 0.0015 0.0116 0.0009 0.0094 0.0128 0.0019 0.0079 0.0166 0.0006 0.0018 0.003 0.0072 0.0024
Bud Site 0.0018 0.0076 0.0022 0.002 0.0012 0.0001 0.0048 0.0026 0.0217 0.0098 0.0005 0.0003 0.0111 0.0139 0.0026 0.0005 0.0037 0.0001
Cell Periphery 0.4536 0.5053 0.5182 0.3275 0.3832 0.3868 0.4195 0.5222 0.5173 0.451 0.4614 0.3788 0.3843 0.4488 0.5828 0.4752 0.3373 0.4055
Cytoplasm 0.0259 0.0363 0.0129 0.0161 0.0279 0.0067 0.0032 0.0142 0.0078 0.0078 0.0082 0.005 0.0078 0.0127 0.0236 0.0075 0.0114 0.0035
Cytoplasmic Foci 0.0055 0.0001 0.0009 0.0052 0.006 0.0017 0.0007 0.0001 0.0025 0.0012 0.0063 0.0014 0.0125 0 0.0041 0 0.0117 0
Eisosomes 0.0166 0.0028 0.0045 0.0006 0.0008 0.0014 0.004 0.0015 0.0038 0.003 0.0009 0.001 0.0114 0.0008 0.0091 0.0027 0.0074 0.0013
Endoplasmic Reticulum 0.3628 0.4005 0.3743 0.5318 0.4397 0.5628 0.3757 0.4145 0.3637 0.4401 0.4253 0.5576 0.4192 0.4861 0.3035 0.424 0.398 0.5669
Endosome 0.0045 0 0.0019 0.0077 0.0145 0.0023 0.0033 0.0001 0.0008 0.0003 0.018 0.0018 0.0013 0 0.0005 0.0001 0.0486 0
Golgi 0.0011 0.0001 0.0009 0.0014 0.0025 0.0005 0.0011 0.0002 0.001 0.0004 0.0077 0.0013 0.0043 0.0001 0.0016 0.0002 0.0265 0.0002
Lipid Particles 0.0042 0.0053 0.004 0.0056 0.0138 0.0029 0.0037 0.0057 0.003 0.0031 0.0122 0.0025 0.022 0.0015 0.0068 0.0011 0.0323 0.0016
Mitochondria 0.0109 0 0.0047 0.0068 0.0019 0.0005 0.0643 0 0.0024 0.0041 0.0013 0.0089 0.0012 0 0.0041 0.0589 0.0178 0
None 0.0072 0.0001 0.0041 0.0092 0.0051 0.0005 0.0019 0.0001 0.0073 0.0021 0.0039 0.0004 0.0007 0 0.0002 0.0001 0.0029 0.0001
Nuclear Periphery 0.0339 0.0231 0.0363 0.0304 0.0151 0.0125 0.0488 0.023 0.0253 0.0197 0.0164 0.0111 0.0295 0.0239 0.0236 0.0129 0.0091 0.0095
Nucleolus 0.0031 0 0.0014 0.0032 0.0027 0.0001 0.0021 0 0.0004 0.0104 0.0001 0.0001 0.0002 0 0.0001 0 0.0004 0
Nucleus 0.0105 0.0002 0.0013 0.01 0.0057 0.0004 0.0149 0.0002 0.0006 0.002 0.0004 0.0002 0.0005 0.0002 0.0003 0.0001 0.0008 0.0001
Peroxisomes 0.0002 0 0.0003 0.0008 0.0065 0.0002 0.0006 0 0.0014 0.0007 0.0033 0.0009 0.0243 0 0.0089 0 0.0025 0
Punctate Nuclear 0.0037 0 0.0011 0.0077 0.0385 0.0047 0.0044 0 0.0003 0.0001 0.0023 0.0016 0.0005 0 0.0001 0 0.001 0
Vacuole 0.0327 0.017 0.0196 0.0131 0.0247 0.0128 0.0236 0.0138 0.0181 0.0211 0.0073 0.0086 0.0167 0.0105 0.0116 0.0108 0.0056 0.0069
Vacuole Periphery 0.0025 0.0001 0.0015 0.0024 0.0007 0.0003 0.0032 0.0001 0.0006 0.0024 0.0005 0.0003 0.0004 0.0001 0.0002 0.0002 0.0013 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 10.0253 15.6834 25.3538 28.2589 16.6539 11.2722 12.3179 12.4228 17.5583 11.3927
Translational Efficiency 0.9387 1.2787 1.2822 1.2024 0.7625 1.0605 0.8599 1.1064 1.0391 0.6184

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency
Standard name
Human Ortholog
Description Polyamine transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; recognizes spermine, putrescine, and spermidine; catalyzes uptake of polyamines at alkaline pH and excretion at acidic pH; during oxidative stress exports spermine, spermidine from the cell, which controls timing of expression of stress-responsive genes; phosphorylation enhances activity and sorting to the plasma membrane
Localization
Cell Percentages cell periphery (74%)
Cell Cycle Regulation No
Subcompartmental Group cell periphery-1

Tpo1

Tpo1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Tpo1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available