ORF | |
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Human Ortholog | |
Description | Protein of unknown function; may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YLL032C is not an essential gene |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 1 |
Bud | 0 | 1 | 1 | 0 | 1 | 5 | 4 | 2 | 9 | 11 | 11 | 5 | 2 | 1 | 0 | 1 | 0 | 2 | 0 | 0 | 1 | 3 | 0 | 3 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 2 | 2 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 3 | 10 | 17 | 2 | 5 | 6 | 10 | 7 | 9 | 7 | 10 | 18 | 1 | 4 | 1 | 7 | 7 | 3 | 0 | 0 | 1 | 0 | 0 | 1 |
Cytoplasm | 115 | 318 | 363 | 255 | 358 | 352 | 463 | 391 | 296 | 304 | 239 | 276 | 298 | 280 | 399 | 180 | 146 | 120 | 107 | 311 | 334 | 112 | 242 | 217 |
Endoplasmic Reticulum | 0 | 5 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 3 | 2 | 1 | 1 | 9 | 3 | 3 | 1 | 2 | 3 | 13 | 16 | 7 |
Endosome | 0 | 0 | 0 | 1 | 1 | 3 | 1 | 1 | 0 | 1 | 1 | 3 | 1 | 1 | 1 | 3 | 3 | 2 | 1 | 2 | 0 | 0 | 0 | 3 |
Golgi | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 1 | 0 | 0 | 1 | 0 | 0 | 1 |
Mitochondria | 2 | 4 | 8 | 17 | 13 | 64 | 115 | 55 | 159 | 152 | 167 | 107 | 4 | 2 | 4 | 5 | 3 | 5 | 1 | 5 | 11 | 11 | 19 | 11 |
Nucleus | 0 | 2 | 0 | 0 | 1 | 2 | 8 | 3 | 0 | 3 | 0 | 5 | 1 | 3 | 0 | 1 | 2 | 1 | 1 | 1 | 5 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 4 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 |
Nucleolus | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 4 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 |
Vac/Vac Membrane | 0 | 0 | 11 | 2 | 9 | 9 | 4 | 21 | 15 | 17 | 14 | 14 | 0 | 1 | 1 | 13 | 8 | 11 | 2 | 6 | 4 | 6 | 16 | 7 |
Unique Cell Count | 118 | 327 | 377 | 262 | 374 | 393 | 532 | 436 | 410 | 414 | 349 | 367 | 303 | 285 | 401 | 200 | 160 | 133 | 121 | 333 | 375 | 152 | 308 | 265 |
Labelled Cell Count | 121 | 343 | 402 | 278 | 389 | 442 | 608 | 485 | 489 | 498 | 446 | 432 | 310 | 293 | 407 | 222 | 173 | 148 | 121 | 333 | 375 | 152 | 308 | 265 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 6.2 | 6.0 | 6.7 | 5.8 | 6.5 | 5.1 | 5.6 | 5.8 | 4.9 | 4.6 | 4.6 | 4.8 | 5.8 | 5.9 | 5.7 | 9.3 | 9.3 | 8.0 | 5.8 | 5.6 | 5.9 |
Std Deviation (1e-4) | 0.8 | 0.9 | 1.4 | 1.1 | 1.7 | 1.0 | 1.3 | 1.5 | 1.0 | 1.0 | 1.1 | 0.8 | 0.9 | 1.0 | 0.9 | 1.8 | 1.4 | 1.8 | 1.5 | 1.5 | 1.1 |
Intensity Change (Log2) | – | – | – | -0.2 | -0.03 | -0.39 | -0.26 | -0.19 | -0.46 | -0.52 | -0.55 | -0.47 | -0.19 | -0.19 | -0.23 | 0.47 | 0.47 | 0.27 | -0.19 | -0.24 | -0.18 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | -2.7 | -2.6 | -2.4 | -2.3 | -2.4 | 0 | 0 | 0 | 0 | -3.4 | -2.3 | -3.9 | -0.6 | -0.1 | -1.2 |
Cytoplasm | 0.7 | -0.4 | -3.6 | -4.8 | -3.6 | -9.1 | -8.8 | -9.9 | -8.3 | 1.6 | 1.5 | 3.2 | -3.1 | -2.4 | -2.7 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 2.8 | 1.1 | 6.7 | 8.5 | 5.6 | 0 | 0 | 0 | 0 | -0.8 | 0 | -1.3 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | -1.9 | -0.4 | -0.5 | -2.5 | 1.4 | 0.6 | 0.9 | 0.8 | 0.7 | 0 | -2.5 | -3.0 | 2.1 | 1.2 | 2.6 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 3.6331 | 4.5265 | 4.1086 | 3.921 | 3.9443 | 4.2656 | 1.7862 | 3.1311 | 2.569 | 3.0033 | 2.5073 | 2.8468 | 1.5297 | 2.3342 | 1.8593 | 2.3134 | 1.5656 | 2.1549 |
Actin | 0.0346 | 0.0073 | 0.0155 | 0.0022 | 0.0055 | 0.0019 | 0.0228 | 0.0037 | 0.0126 | 0.0538 | 0.0142 | 0.0047 | 0.0223 | 0.0019 | 0.0128 | 0.0026 | 0.0003 | 0.0013 |
Bud | 0.0007 | 0.0012 | 0.0005 | 0.0004 | 0.0123 | 0.0006 | 0.0005 | 0.0028 | 0.0003 | 0.0051 | 0.001 | 0.0014 | 0.0007 | 0.0013 | 0.0046 | 0.0023 | 0.0059 | 0.0022 |
Bud Neck | 0.0042 | 0.0007 | 0.0058 | 0.0005 | 0.0005 | 0.009 | 0.0006 | 0.0004 | 0.0003 | 0.0026 | 0.0006 | 0.0011 | 0.003 | 0.0003 | 0.007 | 0.0005 | 0.001 | 0.0026 |
Bud Periphery | 0.001 | 0.0009 | 0.0006 | 0.0004 | 0.0218 | 0.0014 | 0.0006 | 0.0019 | 0.0002 | 0.0059 | 0.0016 | 0.0015 | 0.0007 | 0.0006 | 0.0031 | 0.0042 | 0.003 | 0.0017 |
Bud Site | 0.0163 | 0.0178 | 0.0046 | 0.001 | 0.0026 | 0.0003 | 0.0013 | 0.0072 | 0.0036 | 0.0211 | 0.0017 | 0.0003 | 0.003 | 0.0116 | 0.0085 | 0.0006 | 0.0016 | 0.0005 |
Cell Periphery | 0.0011 | 0.0006 | 0.0004 | 0.0001 | 0.0003 | 0.0002 | 0.0003 | 0.0004 | 0.0002 | 0.0006 | 0.0002 | 0.0001 | 0.0006 | 0.0001 | 0.0004 | 0.0001 | 0.0003 | 0.0001 |
Cytoplasm | 0.5472 | 0.8291 | 0.6976 | 0.794 | 0.7684 | 0.8349 | 0.7813 | 0.8953 | 0.9058 | 0.7182 | 0.637 | 0.8884 | 0.6343 | 0.8669 | 0.7419 | 0.7938 | 0.8208 | 0.8306 |
Cytoplasmic Foci | 0.0283 | 0.0088 | 0.0147 | 0.0183 | 0.0055 | 0.0104 | 0.021 | 0.005 | 0.0085 | 0.0218 | 0.0553 | 0.0208 | 0.048 | 0.0192 | 0.0302 | 0.0239 | 0.0312 | 0.0215 |
Eisosomes | 0.0004 | 0.0001 | 0.0002 | 0.0001 | 0 | 0.0001 | 0.0002 | 0 | 0.0002 | 0.0004 | 0.0001 | 0 | 0.0001 | 0 | 0.0003 | 0 | 0 | 0.0001 |
Endoplasmic Reticulum | 0.0132 | 0.0023 | 0.0039 | 0.0047 | 0.0025 | 0.003 | 0.0117 | 0.0028 | 0.0052 | 0.0243 | 0.029 | 0.0059 | 0.0146 | 0.0027 | 0.0042 | 0.007 | 0.0042 | 0.007 |
Endosome | 0.0304 | 0.0027 | 0.0125 | 0.0387 | 0.016 | 0.0073 | 0.0282 | 0.0031 | 0.0083 | 0.0459 | 0.0764 | 0.023 | 0.0487 | 0.0055 | 0.0158 | 0.0435 | 0.0224 | 0.0265 |
Golgi | 0.0109 | 0.0011 | 0.006 | 0.0065 | 0.001 | 0.0019 | 0.0113 | 0.0004 | 0.0013 | 0.0148 | 0.0354 | 0.0042 | 0.013 | 0.0009 | 0.006 | 0.0041 | 0.0011 | 0.0044 |
Lipid Particles | 0.0114 | 0.0004 | 0.0046 | 0.0142 | 0.0002 | 0.0014 | 0.0014 | 0.0001 | 0.0006 | 0.0059 | 0.0164 | 0.0006 | 0.0082 | 0.0003 | 0.0045 | 0.0003 | 0.0074 | 0.0024 |
Mitochondria | 0.0144 | 0.0003 | 0.0076 | 0.0359 | 0.0009 | 0.0049 | 0.006 | 0.0001 | 0.0009 | 0.0386 | 0.0523 | 0.0164 | 0.0164 | 0.0004 | 0.0118 | 0.001 | 0.0008 | 0.0098 |
None | 0.2522 | 0.1206 | 0.2113 | 0.0646 | 0.1291 | 0.1107 | 0.0836 | 0.0747 | 0.047 | 0.0127 | 0.0566 | 0.022 | 0.1462 | 0.0817 | 0.1294 | 0.0972 | 0.0843 | 0.083 |
Nuclear Periphery | 0.0091 | 0.0005 | 0.0018 | 0.002 | 0.001 | 0.0042 | 0.0078 | 0.0004 | 0.001 | 0.0046 | 0.0038 | 0.0024 | 0.0036 | 0.0004 | 0.0011 | 0.0018 | 0.0012 | 0.0011 |
Nucleolus | 0.0012 | 0.0001 | 0.0002 | 0.0005 | 0.0001 | 0.0001 | 0.0004 | 0 | 0.0001 | 0.0004 | 0.0002 | 0.0001 | 0.0006 | 0.0001 | 0.0004 | 0.0001 | 0.002 | 0.0001 |
Nucleus | 0.0051 | 0.0016 | 0.0013 | 0.0019 | 0.0008 | 0.001 | 0.0083 | 0.0006 | 0.0012 | 0.0027 | 0.0016 | 0.001 | 0.0093 | 0.0013 | 0.0029 | 0.002 | 0.0036 | 0.0012 |
Peroxisomes | 0.0113 | 0.0017 | 0.0042 | 0.0068 | 0.0001 | 0.0043 | 0.0032 | 0 | 0.0004 | 0.0095 | 0.0097 | 0.0007 | 0.0163 | 0.0026 | 0.0085 | 0.0029 | 0.0009 | 0.0009 |
Punctate Nuclear | 0.003 | 0.0009 | 0.0031 | 0.0005 | 0.0002 | 0.001 | 0.0016 | 0.0001 | 0.0006 | 0.0019 | 0.0011 | 0.0003 | 0.0055 | 0.0005 | 0.0035 | 0.0006 | 0.0031 | 0.0006 |
Vacuole | 0.0031 | 0.001 | 0.0026 | 0.005 | 0.0194 | 0.0011 | 0.0056 | 0.001 | 0.0014 | 0.0075 | 0.0038 | 0.0034 | 0.0042 | 0.0014 | 0.0026 | 0.0103 | 0.0046 | 0.0017 |
Vacuole Periphery | 0.0009 | 0.0001 | 0.0009 | 0.0015 | 0.0116 | 0.0005 | 0.0024 | 0.0001 | 0.0003 | 0.0018 | 0.0022 | 0.0016 | 0.0007 | 0.0002 | 0.0005 | 0.0013 | 0.0006 | 0.0009 |
R1 | R2 | |||||||||
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G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 6.9018 | 13.1358 | 5.0213 | 9.9211 | 6.6392 | 5.3404 | 14.0865 | 16.4343 | 10.8473 | 10.6293 |
Translational Efficiency | 0.5253 | 0.9081 | 1.4691 | 0.5197 | 0.6781 | 0.5971 | 0.7801 | 0.5286 | 0.6016 | 0.5748 |
Dataset | Hit |
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Protein Concentration | ✔ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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