Standard name | |
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Human Ortholog | |
Description | Splicing factor associated with the spliceosome; contains a U-box, a motif found in a class of ubiquitin ligases, and a WD40 domain; relocalizes to the cytosol in response to hypoxia |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 3 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 1 | 1 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 1 | 1 | 2 | 3 | 3 | 7 | 6 | 5 | 1 | 2 | 1 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0 | 0 | 2 | 3 | 8 | 4 | 8 | 6 | 2 | 7 | 3 | 3 | 15 | 33 | 46 | 10 | 4 | 2 | 0 | 0 | 0 | 0 | 2 | 0 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 6 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 1 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 4 | 1 | 3 | 0 | 0 | 1 | 1 | 1 | 0 | 4 | 1 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 0 | 0 | 2 | 0 |
Mitochondria | 6 | 6 | 16 | 12 | 20 | 47 | 90 | 86 | 66 | 85 | 64 | 75 | 1 | 1 | 4 | 4 | 2 | 2 | 1 | 1 | 0 | 0 | 1 | 4 |
Nucleus | 159 | 250 | 137 | 75 | 194 | 150 | 228 | 297 | 120 | 179 | 151 | 155 | 146 | 240 | 201 | 196 | 89 | 37 | 160 | 248 | 130 | 107 | 178 | 148 |
Nuclear Periphery | 1 | 1 | 1 | 0 | 1 | 1 | 14 | 6 | 3 | 7 | 6 | 5 | 0 | 1 | 4 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 28 | 20 | 25 | 7 | 17 | 25 | 57 | 60 | 27 | 45 | 30 | 37 | 5 | 22 | 31 | 14 | 10 | 2 | 16 | 9 | 14 | 8 | 23 | 27 |
Peroxisomes | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 6 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 6 | 4 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 3 | 1 |
Vac/Vac Membrane | 1 | 6 | 2 | 0 | 4 | 4 | 3 | 1 | 0 | 3 | 0 | 4 | 0 | 1 | 1 | 2 | 3 | 0 | 4 | 10 | 6 | 8 | 28 | 31 |
Unique Cell Count | 177 | 266 | 152 | 83 | 222 | 176 | 298 | 361 | 158 | 244 | 178 | 197 | 162 | 293 | 269 | 215 | 103 | 41 | 189 | 278 | 154 | 128 | 251 | 224 |
Labelled Cell Count | 195 | 286 | 183 | 97 | 245 | 232 | 402 | 461 | 221 | 336 | 261 | 284 | 169 | 317 | 296 | 235 | 111 | 44 | 189 | 278 | 154 | 128 | 251 | 224 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 8.2 | 8.9 | 6.3 | 6.5 | 6.4 | 5.7 | 5.0 | 5.3 | 5.0 | 4.8 | 4.6 | 4.9 | 9.6 | 9.5 | 9.0 | 11.7 | 12.2 | 13.7 | 9.0 | 9.1 | 9.8 |
Std Deviation (1e-4) | 1.4 | 1.8 | 1.1 | 1.5 | 1.4 | 1.3 | 1.2 | 1.4 | 1.2 | 1.4 | 1.1 | 1.3 | 2.7 | 2.9 | 3.2 | 3.5 | 3.9 | 3.5 | 1.8 | 2.2 | 2.7 |
Intensity Change (Log2) | – | – | – | 0.04 | 0.04 | -0.14 | -0.33 | -0.24 | -0.33 | -0.38 | -0.44 | -0.37 | 0.62 | 0.59 | 0.51 | 0.9 | 0.95 | 1.12 | 0.51 | 0.53 | 0.64 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.1 | 3.7 | 4.9 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0.9 | -0.5 | 3.7 | 4.7 | 3.4 | 6.2 | 0 | 0 | 0 | -3.9 | -5.3 | -4.2 | -3.6 | -2.6 | 0 |
Nucleus | 0.1 | -0.8 | -1.3 | -3.5 | -2.3 | -3.3 | -4.0 | -1.4 | -2.9 | 0 | -2.3 | -3.8 | 0.3 | -0.9 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 2.3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | -1.7 | -2.6 | -0.6 | 0.7 | 0 | 0.2 | 0 | 0 | 0 | -4.0 | -2.9 | -1.4 | -3.0 | -1.5 | -1.9 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Actin | 0.1129 | 0 | 0.0003 | 0 | 0.0001 | 0 | 0.0043 | 0 | 0.0101 | 0.0001 | 0 | 0.0001 | 0.2848 | 0.0001 | 0.0003 | 0 | 0 | 0.004 |
Bud | 0.0003 | 0 | 0 | 0 | 0 | 0 | 0.0004 | 0 | 0.0002 | 0.0001 | 0 | 0.0003 | 0.0037 | 0 | 0 | 0 | 0.0001 | 0.0001 |
Bud Neck | 0.0009 | 0 | 0 | 0 | 0.0002 | 0.0003 | 0.0003 | 0 | 0.0001 | 0.0001 | 0.0002 | 0.001 | 0.0103 | 0 | 0 | 0 | 0.0002 | 0.0008 |
Bud Periphery | 0.0002 | 0 | 0.0001 | 0 | 0.0001 | 0.0001 | 0.0005 | 0 | 0.0003 | 0.0005 | 0.0001 | 0.0011 | 0.006 | 0 | 0 | 0 | 0.0001 | 0.0004 |
Bud Site | 0.0023 | 0 | 0 | 0 | 0 | 0 | 0.0017 | 0 | 0.0016 | 0.0001 | 0.0001 | 0.0002 | 0.124 | 0 | 0 | 0 | 0.0001 | 0.0003 |
Cell Periphery | 0.0001 | 0 | 0 | 0 | 0 | 0.0001 | 0.0002 | 0 | 0.0001 | 0.0001 | 0 | 0.0002 | 0.0022 | 0 | 0 | 0 | 0.0001 | 0.0001 |
Cytoplasm | 0.0044 | 0.0001 | 0 | 0 | 0.0001 | 0 | 0.0018 | 0 | 0.0002 | 0 | 0.0001 | 0.0001 | 0.0331 | 0.0001 | 0.0111 | 0.0001 | 0 | 0.0001 |
Cytoplasmic Foci | 0.0052 | 0 | 0.0001 | 0 | 0 | 0 | 0.0124 | 0 | 0.0017 | 0 | 0 | 0.0001 | 0.0162 | 0 | 0.0004 | 0 | 0 | 0.0003 |
Eisosomes | 0.0003 | 0 | 0 | 0 | 0 | 0 | 0.0001 | 0 | 0.0002 | 0 | 0 | 0 | 0.0033 | 0 | 0 | 0 | 0 | 0.0003 |
Endoplasmic Reticulum | 0.002 | 0 | 0.0001 | 0 | 0 | 0 | 0.0013 | 0 | 0.0005 | 0.0001 | 0 | 0.0001 | 0.0526 | 0 | 0.0016 | 0 | 0 | 0.0002 |
Endosome | 0.0043 | 0 | 0.0002 | 0 | 0.0001 | 0 | 0.0046 | 0 | 0.0034 | 0.0001 | 0 | 0.0004 | 0.0795 | 0 | 0.0008 | 0 | 0 | 0.0022 |
Golgi | 0.0047 | 0 | 0.0001 | 0 | 0 | 0 | 0.0018 | 0 | 0.0041 | 0 | 0 | 0.0001 | 0.0594 | 0 | 0 | 0 | 0 | 0.0022 |
Lipid Particles | 0.0017 | 0 | 0.0025 | 0 | 0 | 0 | 0.0073 | 0 | 0.0028 | 0 | 0 | 0.0002 | 0.0267 | 0 | 0.0002 | 0 | 0 | 0.0008 |
Mitochondria | 0.0011 | 0.0002 | 0.0081 | 0.0001 | 0.0006 | 0.0002 | 0.0046 | 0.0001 | 0.0207 | 0.0021 | 0.0003 | 0.0018 | 0.0241 | 0.0007 | 0.0003 | 0.0002 | 0.0006 | 0.0801 |
None | 0.0018 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0.0044 | 0 | 0.0002 | 0 | 0 | 0.0001 | 0.008 | 0 | 0.0265 | 0 | 0 | 0.0002 |
Nuclear Periphery | 0.004 | 0.0028 | 0.0056 | 0.0005 | 0.0075 | 0.0003 | 0.011 | 0.0016 | 0.0059 | 0.0031 | 0.0042 | 0.0022 | 0.0771 | 0.0103 | 0.005 | 0.0014 | 0.0001 | 0.0031 |
Nucleolus | 0.0308 | 0.0255 | 0.0094 | 0.0062 | 0.1198 | 0.0635 | 0.0163 | 0.0157 | 0.02 | 0.0151 | 0.1443 | 0.0661 | 0.0015 | 0.0547 | 0.0106 | 0.0031 | 0.1814 | 0.0671 |
Nucleus | 0.7976 | 0.9694 | 0.9711 | 0.9931 | 0.8661 | 0.9351 | 0.9079 | 0.9824 | 0.9225 | 0.9762 | 0.8471 | 0.9222 | 0.0591 | 0.9329 | 0.9414 | 0.9951 | 0.8168 | 0.8339 |
Peroxisomes | 0.0019 | 0 | 0.0001 | 0 | 0 | 0 | 0.0069 | 0 | 0.0017 | 0 | 0 | 0 | 0.0091 | 0 | 0 | 0 | 0 | 0.0006 |
Punctate Nuclear | 0.0227 | 0.0017 | 0.0003 | 0.0001 | 0.0047 | 0.0002 | 0.0101 | 0.0001 | 0.0027 | 0.0001 | 0.0033 | 0.0004 | 0.1059 | 0.0007 | 0.0011 | 0.0001 | 0.0001 | 0.0025 |
Vacuole | 0.0004 | 0 | 0.0002 | 0 | 0.0001 | 0.0002 | 0.0013 | 0 | 0.0004 | 0.0007 | 0.0001 | 0.0018 | 0.0045 | 0 | 0.0002 | 0 | 0.0002 | 0.0003 |
Vacuole Periphery | 0.0003 | 0.0001 | 0.0014 | 0 | 0.0003 | 0.0001 | 0.0009 | 0 | 0.0004 | 0.0013 | 0.0001 | 0.0013 | 0.009 | 0.0003 | 0.0002 | 0 | 0.0001 | 0.0005 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 13.8655 | 20.8757 | 22.2678 | 19.2427 | 22.7081 | 14.5872 | 19.2935 | 19.1431 | 16.8479 | 19.6706 |
Translational Efficiency | 1.5855 | 1.1193 | 0.8536 | 0.9999 | 0.9261 | 1.1616 | 1.0249 | 1.0869 | 1.1227 | 1.0183 |
Dataset | Hit |
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Protein Concentration | – |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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