Standard name
Human Ortholog
Description Ribosomal 60S subunit protein L8B; required for processing of 27SA3 pre-rRNA to 27SB pre-rRNA during assembly of large ribosomal subunit; depletion leads to a turnover of pre-rRNA; L8 binds to Domain I of 25S and 5.8 S rRNAs; mutation results in decreased amounts of free 60S subunits; homologous to mammalian ribosomal protein L7A, no bacterial homolog; RPL8B has a paralog, RPL8A, that arose from the whole genome duplication

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0.05 0.06 0.05 0.1 0.13 0.09 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1.0 0.99 0.99 0.97 0.95 0.82 0.81 0.8 0.79 0.67 0.69 0.98 0.96 1.0 0.99 1.0 0.96 0.94 0.94 0.86 0.96 0.93
Endoplasmic Reticulum 0 0 0 0 0 0.14 0.17 0.15 0.13 0.21 0.21 0 0 0.06 0 0.07 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 3 4 2 5 5 8 0 1 0 0 0 0 0 0 0 0 0
Bud 0 2 0 0 1 1 2 2 1 2 1 0 0 0 0 0 0 0 0 0 0 1
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0
Cell Periphery 0 3 1 5 8 17 22 8 19 25 23 0 4 0 0 0 0 0 0 0 1 0
Cytoplasm 109 181 183 307 240 278 311 138 154 126 184 65 117 17 104 105 27 98 161 24 97 113
Endoplasmic Reticulum 0 3 1 8 11 47 65 25 26 40 56 1 4 1 4 7 0 0 4 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 1 0 1 0 3 1 2 1 3 10 0 1 0 1 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 1 3 1 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 1 0 1 0 0 0 0 0 0 0 0 0 0 1
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 1
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 1 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 1 0 0 0 0 2 1 0 0 0 0 0 0 1 1 0 0 0
Unique Cell Count 109 183 184 316 252 338 384 172 195 189 266 66 122 17 105 105 29 105 173 29 102 122
Labelled Cell Count 109 190 185 321 261 350 405 177 208 209 286 66 127 18 109 112 29 105 173 29 102 122


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 143.3 149.0 154.1 124.6 89.1 74.4 67.0 59.6 61.2 54.0 57.9 143.2 127.5 134.7 180.4 209.4 157.5 154.7 146.5
Std Deviation (1e-4) 39.0 31.0 32.6 22.8 20.0 15.3 14.7 13.8 12.5 13.6 13.2 36.1 42.3 18.9 45.6 56.4 49.4 60.9 60.4
Intensity Change (Log2) 0.05 -0.26 -0.74 -1.0 -1.15 -1.32 -1.28 -1.46 -1.36 -0.06 -0.23 -0.15 0.28 0.49 0.08 0.05 -0.02


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 1.9 2.2 1.6 3.4 4.2 3.1 0 0 0 0 0
Cytoplasm 0.6 -1.3 -2.1 -5.6 -5.9 -5.8 -6.1 -8.2 -7.9 -0.3 -1.7 0.4 0.1 1.1
Endoplasmic Reticulum 0 0 1.6 4.5 5.2 4.5 4.3 5.9 6.0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 146.2775 194.0582 167.7297 173.2947 148.1032 97.5011 143.8801 183.2698 171.2399 165.9379 128.7563 183.2385 149.9039 217.3154 191.9293 198.5161 173.3014 132.1751
Actin 0.0077 0.0004 0.0084 0.0004 0.0081 0.0158 0.0049 0.0005 0.0009 0.0008 0.0011 0.0012 0.0218 0.0004 0.0004 0.0005 0.0274 0.0021
Bud 0.0021 0.0013 0.0014 0.0022 0.0014 0.0099 0.0013 0.0008 0.0011 0.0033 0.003 0.0006 0.0012 0.0008 0.0014 0.0024 0.0016 0.0008
Bud Neck 0.0007 0.0011 0.0015 0.0007 0.0008 0.0097 0.0007 0.0007 0.0006 0.0018 0.0014 0.0009 0.0021 0.0005 0.0008 0.001 0.0018 0.0006
Bud Periphery 0.0021 0.0008 0.0018 0.0008 0.0009 0.0069 0.0013 0.0005 0.0007 0.002 0.004 0.0008 0.0012 0.0003 0.0005 0.0008 0.0005 0.0004
Bud Site 0.0008 0.0006 0.0013 0.0003 0.0009 0.0042 0.0011 0.0007 0.0005 0.0004 0.0012 0.0001 0.0053 0.0005 0.0003 0.0003 0.0005 0.0004
Cell Periphery 0.0243 0.0095 0.0257 0.0059 0.0087 0.026 0.0258 0.0074 0.0189 0.0087 0.0155 0.001 0.0095 0.0071 0.0026 0.0212 0.0117 0.0018
Cytoplasm 0.6204 0.8833 0.7069 0.6492 0.7401 0.3984 0.6976 0.9296 0.808 0.6773 0.6597 0.9345 0.7347 0.9453 0.8445 0.7454 0.7303 0.6838
Cytoplasmic Foci 0.0093 0.0012 0.0134 0.0015 0.0056 0.0377 0.0052 0.0009 0.0078 0.0015 0.0193 0.0034 0.0078 0.0006 0.0061 0.0014 0.0065 0.0696
Eisosomes 0.0006 0 0.0003 0 0.0003 0.0024 0.0003 0 0.0002 0 0.0005 0 0.0011 0 0.0001 0 0.0003 0.0015
Endoplasmic Reticulum 0.0704 0.0366 0.016 0.0157 0.0156 0.0112 0.0279 0.007 0.0097 0.006 0.0037 0.0174 0.023 0.0072 0.0069 0.0009 0.0036 0.0007
Endosome 0.0086 0.0025 0.0062 0.0085 0.0258 0.0317 0.0051 0.0018 0.0048 0.0072 0.0114 0.0137 0.0136 0.001 0.0033 0.0025 0.0137 0.0015
Golgi 0.0024 0.0006 0.0025 0.0003 0.0041 0.0128 0.0014 0.0003 0.0006 0.0006 0.0009 0.0035 0.0052 0.0002 0.0005 0.0001 0.0023 0.0005
Lipid Particles 0.005 0.001 0.0047 0.0003 0.0053 0.0123 0.0021 0.0007 0.004 0.0008 0.0094 0.0001 0.0097 0.0002 0.001 0.0007 0.0029 0.0219
Mitochondria 0.0265 0.0015 0.0097 0.0007 0.0063 0.0606 0.006 0.0004 0.0034 0.001 0.0297 0.0054 0.0129 0.0008 0.0096 0.0018 0.0017 0.1146
None 0.0033 0.0002 0.0055 0.0005 0.0051 0.053 0.0043 0.0003 0.0043 0.0003 0.0075 0.0008 0.0073 0.0003 0.0019 0.0005 0.0074 0.007
Nuclear Periphery 0.0408 0.0063 0.0145 0.016 0.0307 0.005 0.0498 0.0035 0.0088 0.0172 0.0085 0.0026 0.0269 0.0045 0.0108 0.0125 0.0138 0.0025
Nucleolus 0.0107 0.0001 0.0072 0.0004 0.0019 0.1284 0.0108 0.0001 0.0013 0.0007 0.0201 0 0.0031 0.0002 0.0064 0.0027 0.0047 0.0492
Nucleus 0.0113 0.0023 0.0066 0.0054 0.0083 0.0308 0.0205 0.0025 0.0053 0.01 0.0119 0.0015 0.0162 0.0056 0.007 0.0141 0.0115 0.0066
Peroxisomes 0.0029 0.0001 0.0021 0.0001 0.0012 0.0034 0.0004 0 0.0004 0.0001 0.0023 0.0001 0.0044 0 0.0006 0.0002 0.0011 0.0097
Punctate Nuclear 0.0022 0 0.0011 0 0.0052 0.0034 0.0009 0 0.0015 0.0001 0.0036 0 0.0091 0 0.0002 0.0002 0.0061 0.0026
Vacuole 0.1121 0.0452 0.1329 0.2193 0.0781 0.0857 0.1065 0.0368 0.0984 0.2011 0.1306 0.0078 0.0662 0.021 0.0727 0.1337 0.1016 0.0166
Vacuole Periphery 0.0359 0.0056 0.0303 0.0715 0.0456 0.0508 0.026 0.0055 0.0188 0.0591 0.055 0.0046 0.0177 0.0034 0.0223 0.0571 0.049 0.0059

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 2021.477 2365.5117 2700.8448 2173.2994 2594.9559 2218.4603 1992.3842 1832.1087 2071.7408 1801.798
Translational Efficiency 1.5129 1.4099 1.3121 1.4838 1.4138 1.308 1.3572 1.4977 1.2613 1.5498

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency
Standard name
Human Ortholog
Description Ribosomal 60S subunit protein L8B; required for processing of 27SA3 pre-rRNA to 27SB pre-rRNA during assembly of large ribosomal subunit; depletion leads to a turnover of pre-rRNA; L8 binds to Domain I of 25S and 5.8 S rRNAs; mutation results in decreased amounts of free 60S subunits; homologous to mammalian ribosomal protein L7A, no bacterial homolog; RPL8B has a paralog, RPL8A, that arose from the whole genome duplication
Localization
Cell Percentages cytoplasm (99%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-1

Rpl8b

Rpl8b


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Rpl8b-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available