Standard name | |
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Human Ortholog | |
Description | Ribosomal 60S subunit protein L8B; required for processing of 27SA3 pre-rRNA to 27SB pre-rRNA during assembly of large ribosomal subunit; depletion leads to a turnover of pre-rRNA; L8 binds to Domain I of 25S and 5.8 S rRNAs; mutation results in decreased amounts of free 60S subunits; homologous to mammalian ribosomal protein L7A, no bacterial homolog; RPL8B has a paralog, RPL8A, that arose from the whole genome duplication |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | – | 0 | 0 | 0 | 0 | 0 | 3 | 4 | 2 | 5 | 5 | 8 | – | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | – | 0 | 2 | 0 | 0 | 1 | 1 | 2 | 2 | 1 | 2 | 1 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Bud Neck | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | – | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | – | 0 | 3 | 1 | 5 | 8 | 17 | 22 | 8 | 19 | 25 | 23 | – | 0 | 4 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 |
Cytoplasm | – | 109 | 181 | 183 | 307 | 240 | 278 | 311 | 138 | 154 | 126 | 184 | – | 65 | 117 | 17 | 104 | 105 | 27 | 98 | 161 | 24 | 97 | 113 |
Endoplasmic Reticulum | – | 0 | 3 | 1 | 8 | 11 | 47 | 65 | 25 | 26 | 40 | 56 | – | 1 | 4 | 1 | 4 | 7 | 0 | 0 | 4 | 0 | 0 | 0 |
Endosome | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | – | 0 | 1 | 0 | 1 | 0 | 3 | 1 | 2 | 1 | 3 | 10 | – | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 3 | 1 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Nuclear Periphery | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Peroxisomes | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | – | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 2 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | – | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 2 | 1 | – | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 |
Unique Cell Count | 109 | 183 | 184 | 316 | 252 | 338 | 384 | 172 | 195 | 189 | 266 | 66 | 122 | 17 | 105 | 105 | 29 | 105 | 173 | 29 | 102 | 122 | ||
Labelled Cell Count | 109 | 190 | 185 | 321 | 261 | 350 | 405 | 177 | 208 | 209 | 286 | 66 | 127 | 18 | 109 | 112 | 29 | 105 | 173 | 29 | 102 | 122 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | – | 143.3 | 149.0 | 154.1 | 124.6 | 89.1 | 74.4 | 67.0 | 59.6 | 61.2 | 54.0 | 57.9 | – | 143.2 | 127.5 | 134.7 | 180.4 | 209.4 | 157.5 | 154.7 | 146.5 |
Std Deviation (1e-4) | – | 39.0 | 31.0 | 32.6 | 22.8 | 20.0 | 15.3 | 14.7 | 13.8 | 12.5 | 13.6 | 13.2 | – | 36.1 | 42.3 | 18.9 | 45.6 | 56.4 | 49.4 | 60.9 | 60.4 |
Intensity Change (Log2) | – | – | – | 0.05 | -0.26 | -0.74 | -1.0 | -1.15 | -1.32 | -1.28 | -1.46 | -1.36 | – | -0.06 | -0.23 | -0.15 | 0.28 | 0.49 | 0.08 | 0.05 | -0.02 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 1.9 | 2.2 | 1.6 | 3.4 | 4.2 | 3.1 | – | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0.6 | -1.3 | -2.1 | -5.6 | -5.9 | -5.8 | -6.1 | -8.2 | -7.9 | – | -0.3 | -1.7 | 0.4 | 0.1 | 1.1 |
Endoplasmic Reticulum | 0 | 0 | 1.6 | 4.5 | 5.2 | 4.5 | 4.3 | 5.9 | 6.0 | – | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 146.2775 | 194.0582 | 167.7297 | 173.2947 | 148.1032 | 97.5011 | 143.8801 | 183.2698 | 171.2399 | 165.9379 | 128.7563 | 183.2385 | 149.9039 | 217.3154 | 191.9293 | 198.5161 | 173.3014 | 132.1751 |
Actin | 0.0077 | 0.0004 | 0.0084 | 0.0004 | 0.0081 | 0.0158 | 0.0049 | 0.0005 | 0.0009 | 0.0008 | 0.0011 | 0.0012 | 0.0218 | 0.0004 | 0.0004 | 0.0005 | 0.0274 | 0.0021 |
Bud | 0.0021 | 0.0013 | 0.0014 | 0.0022 | 0.0014 | 0.0099 | 0.0013 | 0.0008 | 0.0011 | 0.0033 | 0.003 | 0.0006 | 0.0012 | 0.0008 | 0.0014 | 0.0024 | 0.0016 | 0.0008 |
Bud Neck | 0.0007 | 0.0011 | 0.0015 | 0.0007 | 0.0008 | 0.0097 | 0.0007 | 0.0007 | 0.0006 | 0.0018 | 0.0014 | 0.0009 | 0.0021 | 0.0005 | 0.0008 | 0.001 | 0.0018 | 0.0006 |
Bud Periphery | 0.0021 | 0.0008 | 0.0018 | 0.0008 | 0.0009 | 0.0069 | 0.0013 | 0.0005 | 0.0007 | 0.002 | 0.004 | 0.0008 | 0.0012 | 0.0003 | 0.0005 | 0.0008 | 0.0005 | 0.0004 |
Bud Site | 0.0008 | 0.0006 | 0.0013 | 0.0003 | 0.0009 | 0.0042 | 0.0011 | 0.0007 | 0.0005 | 0.0004 | 0.0012 | 0.0001 | 0.0053 | 0.0005 | 0.0003 | 0.0003 | 0.0005 | 0.0004 |
Cell Periphery | 0.0243 | 0.0095 | 0.0257 | 0.0059 | 0.0087 | 0.026 | 0.0258 | 0.0074 | 0.0189 | 0.0087 | 0.0155 | 0.001 | 0.0095 | 0.0071 | 0.0026 | 0.0212 | 0.0117 | 0.0018 |
Cytoplasm | 0.6204 | 0.8833 | 0.7069 | 0.6492 | 0.7401 | 0.3984 | 0.6976 | 0.9296 | 0.808 | 0.6773 | 0.6597 | 0.9345 | 0.7347 | 0.9453 | 0.8445 | 0.7454 | 0.7303 | 0.6838 |
Cytoplasmic Foci | 0.0093 | 0.0012 | 0.0134 | 0.0015 | 0.0056 | 0.0377 | 0.0052 | 0.0009 | 0.0078 | 0.0015 | 0.0193 | 0.0034 | 0.0078 | 0.0006 | 0.0061 | 0.0014 | 0.0065 | 0.0696 |
Eisosomes | 0.0006 | 0 | 0.0003 | 0 | 0.0003 | 0.0024 | 0.0003 | 0 | 0.0002 | 0 | 0.0005 | 0 | 0.0011 | 0 | 0.0001 | 0 | 0.0003 | 0.0015 |
Endoplasmic Reticulum | 0.0704 | 0.0366 | 0.016 | 0.0157 | 0.0156 | 0.0112 | 0.0279 | 0.007 | 0.0097 | 0.006 | 0.0037 | 0.0174 | 0.023 | 0.0072 | 0.0069 | 0.0009 | 0.0036 | 0.0007 |
Endosome | 0.0086 | 0.0025 | 0.0062 | 0.0085 | 0.0258 | 0.0317 | 0.0051 | 0.0018 | 0.0048 | 0.0072 | 0.0114 | 0.0137 | 0.0136 | 0.001 | 0.0033 | 0.0025 | 0.0137 | 0.0015 |
Golgi | 0.0024 | 0.0006 | 0.0025 | 0.0003 | 0.0041 | 0.0128 | 0.0014 | 0.0003 | 0.0006 | 0.0006 | 0.0009 | 0.0035 | 0.0052 | 0.0002 | 0.0005 | 0.0001 | 0.0023 | 0.0005 |
Lipid Particles | 0.005 | 0.001 | 0.0047 | 0.0003 | 0.0053 | 0.0123 | 0.0021 | 0.0007 | 0.004 | 0.0008 | 0.0094 | 0.0001 | 0.0097 | 0.0002 | 0.001 | 0.0007 | 0.0029 | 0.0219 |
Mitochondria | 0.0265 | 0.0015 | 0.0097 | 0.0007 | 0.0063 | 0.0606 | 0.006 | 0.0004 | 0.0034 | 0.001 | 0.0297 | 0.0054 | 0.0129 | 0.0008 | 0.0096 | 0.0018 | 0.0017 | 0.1146 |
None | 0.0033 | 0.0002 | 0.0055 | 0.0005 | 0.0051 | 0.053 | 0.0043 | 0.0003 | 0.0043 | 0.0003 | 0.0075 | 0.0008 | 0.0073 | 0.0003 | 0.0019 | 0.0005 | 0.0074 | 0.007 |
Nuclear Periphery | 0.0408 | 0.0063 | 0.0145 | 0.016 | 0.0307 | 0.005 | 0.0498 | 0.0035 | 0.0088 | 0.0172 | 0.0085 | 0.0026 | 0.0269 | 0.0045 | 0.0108 | 0.0125 | 0.0138 | 0.0025 |
Nucleolus | 0.0107 | 0.0001 | 0.0072 | 0.0004 | 0.0019 | 0.1284 | 0.0108 | 0.0001 | 0.0013 | 0.0007 | 0.0201 | 0 | 0.0031 | 0.0002 | 0.0064 | 0.0027 | 0.0047 | 0.0492 |
Nucleus | 0.0113 | 0.0023 | 0.0066 | 0.0054 | 0.0083 | 0.0308 | 0.0205 | 0.0025 | 0.0053 | 0.01 | 0.0119 | 0.0015 | 0.0162 | 0.0056 | 0.007 | 0.0141 | 0.0115 | 0.0066 |
Peroxisomes | 0.0029 | 0.0001 | 0.0021 | 0.0001 | 0.0012 | 0.0034 | 0.0004 | 0 | 0.0004 | 0.0001 | 0.0023 | 0.0001 | 0.0044 | 0 | 0.0006 | 0.0002 | 0.0011 | 0.0097 |
Punctate Nuclear | 0.0022 | 0 | 0.0011 | 0 | 0.0052 | 0.0034 | 0.0009 | 0 | 0.0015 | 0.0001 | 0.0036 | 0 | 0.0091 | 0 | 0.0002 | 0.0002 | 0.0061 | 0.0026 |
Vacuole | 0.1121 | 0.0452 | 0.1329 | 0.2193 | 0.0781 | 0.0857 | 0.1065 | 0.0368 | 0.0984 | 0.2011 | 0.1306 | 0.0078 | 0.0662 | 0.021 | 0.0727 | 0.1337 | 0.1016 | 0.0166 |
Vacuole Periphery | 0.0359 | 0.0056 | 0.0303 | 0.0715 | 0.0456 | 0.0508 | 0.026 | 0.0055 | 0.0188 | 0.0591 | 0.055 | 0.0046 | 0.0177 | 0.0034 | 0.0223 | 0.0571 | 0.049 | 0.0059 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 2021.477 | 2365.5117 | 2700.8448 | 2173.2994 | 2594.9559 | 2218.4603 | 1992.3842 | 1832.1087 | 2071.7408 | 1801.798 |
Translational Efficiency | 1.5129 | 1.4099 | 1.3121 | 1.4838 | 1.4138 | 1.308 | 1.3572 | 1.4977 | 1.2613 | 1.5498 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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