ORF
Human Ortholog
Description Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; predicted to be palmitoylated
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.05 0 0.06 0.09 0.11 0.21 0.2 0.25 0.29 – 0.21 0.26 0 0 0 0.09 0.13 0.14 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0.05 0 0 – 0 0.08 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0.05 0.19 0.14 0.12 0.1 0.07 – 0.07 0.07 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0
Mitochondria 0.1 0.1 0 0.1 0 0.13 0.13 0.11 0.32 – 0.36 0.22 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.08 0.16 0.12 0.05 0 0 0.05 0 0 – 0 0 0.07 0 0.05 0.05 0.05 0.05 0 0.05 0 0.05 0 0
Nuclear Periphery 0.16 0.07 0.05 0 0 0 0 0 0 – 0 0 0.09 0.1 0.11 0.07 0 0.07 0.13 0 0.07 0 0.06 0.06
Nucleolus 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0.05 0 0 0.05 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.81 0.81 0.86 0.8 0.69 0.62 0.54 0.58 0.41 – 0.48 0.47 0.87 0.93 0.9 0.83 0.81 0.79 0.75 0.84 0.82 0.86 0.83 0.8
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 3 0 7 2 1 – 3 6 0 0 0 1 0 1 0 1 1 0 0 0
Bud 0 1 1 1 4 6 8 10 9 – 11 9 1 0 0 0 0 0 0 0 0 0 4 6
Bud Neck 0 0 0 0 0 0 0 0 0 – 0 1 0 0 0 0 0 0 0 0 0 0 0 1
Bud Site 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 1 0 0 1 5 8 3 – 0 5 0 0 0 0 1 2 0 0 0 0 0 0
Cytoplasm 8 3 16 15 30 72 103 91 92 – 66 112 16 13 20 17 16 21 1 1 3 0 0 1
Endoplasmic Reticulum 1 0 1 1 6 4 27 13 11 – 12 32 2 2 3 7 1 4 0 0 2 0 4 3
Endosome 1 0 2 9 53 46 59 38 24 – 23 28 3 8 9 6 0 4 4 3 8 11 20 23
Golgi 0 2 0 0 0 1 7 2 2 – 0 2 0 3 0 8 8 5 1 0 2 0 0 4
Mitochondria 15 14 10 17 11 45 69 39 102 – 113 94 15 4 15 7 3 3 1 1 8 2 4 5
Nucleus 13 23 33 8 9 7 24 15 9 – 8 15 30 19 30 9 6 7 5 8 6 15 16 17
Nuclear Periphery 25 10 13 5 2 4 11 3 7 – 7 3 37 54 72 12 1 11 21 4 20 5 29 32
Nucleolus 3 4 0 2 6 9 16 1 4 – 9 4 3 5 6 3 2 4 1 0 0 2 3 3
Peroxisomes 0 0 0 0 0 2 0 1 0 – 0 2 0 0 1 0 0 0 0 0 0 0 1 0
SpindlePole 0 0 0 0 15 11 19 17 8 – 10 10 0 0 0 2 0 1 0 1 0 0 1 2
Vac/Vac Membrane 125 119 243 132 195 208 274 214 132 – 151 200 374 498 570 152 101 118 126 129 253 266 437 410
Unique Cell Count 154 147 282 164 281 336 512 370 322 317 424 429 537 630 183 125 149 168 155 311 309 527 515
Labelled Cell Count 191 176 320 190 334 416 629 454 404 413 523 481 606 726 224 139 181 168 155 311 309 527 515


Vacuole Periphery

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 7.8 7.8 7.7 6.8 7.6 6.6 6.8 7.0 6.2 – 6.1 6.4 8.7 8.9 9.0 12.5 12.5 12.5 7.3 7.6 8.4
Std Deviation (1e-4) 1.4 1.9 1.1 1.2 1.3 1.5 1.3 1.3 1.1 – 1.3 1.4 5.0 1.4 1.6 3.1 3.0 3.4 1.5 1.5 1.9
Intensity Change (Log2) – – – -0.19 -0.01 -0.22 -0.19 -0.15 -0.31 – -0.35 -0.26 0.17 0.2 0.22 0.7 0.7 0.7 -0.07 -0.02 0.12


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 – 2.7 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Cytoplasm 1.4 2.2 5.6 5.5 6.5 7.3 – 5.4 7.0 -1.2 -2.4 -1.8 1.5 2.5 3.0
Endoplasmic Reticulum 0 0 0 3.6 2.8 2.7 – 2.9 4.4 0 0 0 0 0 0
Endosome 0 7.3 6.0 5.5 5.0 4.1 – 4.0 3.8 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Mitochondria 2.9 0.2 4.3 4.5 3.4 8.9 – 9.7 6.8 0 0 -1.0 0.2 0 0
Nucleus -2.4 -3.8 -4.8 -3.7 -3.7 -4.3 – -4.4 -4.2 -2.2 -4.6 -3.8 -2.5 -2.2 -2.4
Nuclear Periphery -0.8 -2.9 -2.6 -1.9 -3.1 -1.7 – -1.6 -3.4 2.0 2.7 3.3 0.9 0 1.2
Nucleolus 0 0 0 3.0 0 0 – 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
SpindlePole 0 3.9 3.1 3.3 3.6 0 – 0 0 0 0 0 0 0 0
Vacuole -1.6 -4.8 -6.8 -9.2 -7.8 -11.4 – -9.9 -10.5 0.4 3.0 1.9 -0.9 -1.4 -1.9

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.8362 3.7443 3.3398 2.8605 3.2647 3.0245 2.3198 2.6133 2.2779 2.1973 1.9469 2.1996 3.7329 3.2058 3.6895 3.2604 2.9808 3.2226
Actin 0.0369 0.0003 0.0009 0.0049 0 0.0012 0.0044 0 0.0012 0.001 0.0016 0.0031 0.0002 0 0.0001 0 0 0.0001
Bud 0.0005 0.0002 0.0004 0.0003 0.0001 0.0002 0.0005 0.0002 0.0011 0.0007 0.0002 0.0002 0.0003 0.0023 0.0011 0.0003 0.0003 0.0011
Bud Neck 0.0008 0.0007 0.0003 0.0004 0.0001 0.0003 0.0012 0.0002 0.0005 0.0002 0.0003 0.0003 0.0006 0.0012 0.0013 0.0002 0.0005 0.0008
Bud Periphery 0.0013 0.0006 0.0011 0.0007 0.0003 0.0007 0.001 0.0005 0.0009 0.0004 0.0007 0.0007 0.0011 0.0033 0.0023 0.0017 0.0009 0.0017
Bud Site 0.0028 0.0011 0.0006 0.0013 0.0001 0.0002 0.0029 0.0002 0.0027 0.0005 0.0013 0.0002 0.0008 0.0042 0.0029 0.0001 0.0003 0.0005
Cell Periphery 0.0022 0.0014 0.001 0.0005 0.0004 0.0009 0.0014 0.0005 0.0005 0.0002 0.0005 0.0004 0.0021 0.0013 0.0021 0.0051 0.0007 0.0012
Cytoplasm 0.0056 0.0058 0.0045 0.003 0.0037 0.0012 0.0059 0.0092 0.0022 0.0039 0.0007 0.0014 0.0028 0.0084 0.0095 0.0006 0.0042 0.0059
Cytoplasmic Foci 0.0103 0.0054 0.0064 0.0125 0.0065 0.0011 0.0219 0.0013 0.0078 0.05 0.002 0.0044 0.0061 0.0044 0.014 0.0012 0.0231 0.0112
Eisosomes 0.0002 0 0.0001 0.0006 0 0 0.0001 0 0.0001 0 0.0003 0 0.0001 0 0.0001 0 0 0
Endoplasmic Reticulum 0.0054 0.0073 0.0035 0.0023 0.0027 0.0059 0.0037 0.0042 0.0015 0.0006 0.0016 0.0018 0.0018 0.0041 0.0033 0.0009 0.0016 0.002
Endosome 0.1247 0.1105 0.1511 0.1506 0.2301 0.1123 0.1728 0.0912 0.131 0.1376 0.0973 0.1134 0.1114 0.1305 0.1611 0.1644 0.2032 0.1123
Golgi 0.0136 0.0025 0.0057 0.0202 0.0029 0.0101 0.0128 0.0011 0.0115 0.007 0.0043 0.005 0.003 0.0027 0.004 0.0025 0.0046 0.0026
Lipid Particles 0.0115 0.0055 0.0215 0.0343 0.0063 0.0058 0.0121 0.0007 0.0036 0.0045 0.0137 0.007 0.0147 0.0033 0.0155 0.0186 0.0047 0.0174
Mitochondria 0.0324 0.0092 0.0454 0.0184 0.0051 0.0417 0.0174 0.0081 0.0347 0.0263 0.0424 0.0152 0.0598 0.0049 0.0462 0.0191 0.0243 0.0174
None 0.0162 0.0075 0.037 0.0865 0.0004 0.0003 0.0012 0.0004 0.0004 0.0004 0.001 0.0006 0.0046 0.0091 0.0222 0.0001 0.0012 0.0094
Nuclear Periphery 0.0198 0.0244 0.0133 0.0076 0.0076 0.0285 0.0175 0.0167 0.0169 0.0028 0.0085 0.0158 0.0064 0.0138 0.012 0.0045 0.0054 0.0364
Nucleolus 0.004 0.0032 0.0025 0.0018 0.0003 0.0006 0.0025 0.0005 0.0019 0.0023 0.0019 0.0008 0.0183 0.0264 0.0164 0.0015 0.0041 0.0183
Nucleus 0.0074 0.0046 0.0102 0.0016 0.0009 0.0028 0.0029 0.0022 0.0065 0.0011 0.0012 0.0019 0.0387 0.0197 0.0215 0.0017 0.0039 0.0187
Peroxisomes 0.019 0.0004 0.005 0.0067 0.0002 0.0004 0.0029 0.0001 0.0014 0.0015 0.0052 0.0004 0.0032 0.0005 0.0015 0.0011 0.0012 0.0016
Punctate Nuclear 0.0034 0.0003 0.0009 0.001 0.0001 0 0.0017 0 0.0001 0.0077 0.001 0.0004 0.0004 0.0008 0.0026 0 0.0001 0.0029
Vacuole 0.4465 0.479 0.3788 0.4095 0.3933 0.3552 0.4275 0.4721 0.4035 0.3948 0.39 0.3478 0.4237 0.4602 0.389 0.3922 0.4178 0.341
Vacuole Periphery 0.2355 0.3303 0.3099 0.2352 0.3391 0.4305 0.2856 0.3907 0.3701 0.3563 0.4241 0.4791 0.2999 0.2988 0.2711 0.3844 0.2979 0.3975

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 6.1797 5.3338 7.0677 12.7695 7.1835 8.5191 10.4008 10.3998 13.7751 12.7793
Translational Efficiency 0.8241 0.6689 0.8946 0.463 0.6662 0.7291 0.6493 0.4944 0.5695 0.6087

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✔
Gene Expression ✔
Translational Efficiency ✘
ORF
Human Ortholog
Description Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; predicted to be palmitoylated
Localization
Cell Percentages vacuolar membrane (81%)
Cell Cycle Regulation No
Subcompartmental Group vac memb-2

YLR001C

YLR001C


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ylr001c-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available