Standard name
Human Ortholog
Description Cytoplasmic phosphorelay intermediate osmosensor and regulator; part of a two-component signal transducer that mediates osmosensing via a phosphorelay mechanism; required for mitophagy; dephosphorylated form is degraded by the ubiquitin-proteasome system; potential Cdc28p substrate
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0 0.1 0 0 0 0 0 0
Cytoplasm 0.98 0.98 0.98 0.97 0.94 0.72 0.6 0.63 0.57 0.53 0.56 0.52 1.0 0.99 0.99 0.95 0.93 0.96 0.97 0.92 0.95 0.84 0.85 0.87
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0.06 0.47 0.56 0.47 0.62 0.62 0.63 0.58 0 0 0 0 0 0 0 0 0 0.05 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0.05 0 0.08 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 1 0 0 0 0 2 0 0 0 1 0 0 0 1 0 3 5 9
Bud 0 0 0 0 1 2 6 2 2 1 1 1 1 1 0 0 0 0 0 0 0 3 11 10
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 3 2 6
Bud Site 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 2 2 2 3 6 4 2 4 5 4 7 3 13 10 14 3 14 0 0 0 0 2 2
Cytoplasm 106 295 123 131 187 147 159 100 109 126 109 105 276 694 606 169 105 131 101 279 129 316 745 754
Endoplasmic Reticulum 1 2 1 0 2 0 1 0 5 2 7 1 1 0 4 6 5 6 0 0 3 5 17 15
Endosome 0 0 0 0 2 1 2 0 0 0 0 0 0 2 0 0 0 0 0 6 0 4 5 6
Golgi 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 1 0 1 0 0 3 2
Mitochondria 1 4 2 5 12 97 149 74 120 146 124 117 0 0 0 1 2 1 0 6 0 18 37 17
Nucleus 0 0 0 0 0 7 3 2 2 1 1 2 0 1 2 0 1 1 0 0 0 1 1 0
Nuclear Periphery 0 0 0 0 0 3 0 0 1 2 1 2 0 0 0 0 0 0 0 0 0 0 1 1
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 4 3
Vac/Vac Membrane 0 2 0 1 3 7 2 4 4 13 8 16 1 5 4 3 4 1 1 4 0 10 29 34
Unique Cell Count 108 301 125 135 200 205 266 158 192 237 196 201 277 698 612 177 113 136 104 305 137 377 878 871
Labelled Cell Count 109 306 128 139 210 270 327 184 247 297 255 253 282 716 626 195 120 155 104 305 137 377 878 871


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.5 6.3 6.1 5.7 6.2 4.8 5.1 5.9 5.1 5.3 5.3 5.4 6.9 7.4 7.5 8.9 9.1 9.0 6.3 6.7 6.9
Std Deviation (1e-4) 0.7 0.8 0.8 0.8 1.5 1.5 1.8 2.0 1.2 1.6 1.3 1.3 0.8 0.9 1.0 1.6 1.9 1.4 1.1 1.1 1.2
Intensity Change (Log2) – – – -0.09 0.01 -0.34 -0.25 -0.05 -0.25 -0.2 -0.21 -0.17 0.17 0.27 0.3 0.54 0.57 0.55 0.05 0.14 0.18


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 2.4 0 2.9
Cytoplasm -0.7 -2.0 -6.1 -7.9 -7.2 -8.2 -8.8 -8.4 -8.8 1.3 1.2 0.6 -1.4 -2.1 -1.0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 1.9 0 10.3 8.5 10.9 11.0 11.0 10.3 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 3.2 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.2275 2.3779 1.7704 1.2424 1.0621 1.5767 2.8551 3.7616 3.2906 3.0548 3.1828 3.2589 1.571 2.7969 2.0922 2.1734 1.6824 1.9666
Actin 0.0224 0.0006 0.0097 0.0018 0.0257 0.0049 0.0287 0.001 0.0113 0.0031 0.0204 0.0084 0.0158 0.0027 0.0027 0.0307 0.0075 0.0001
Bud 0.0001 0.0003 0.0003 0.0002 0.0002 0.0002 0.0092 0.0014 0.0152 0.0166 0.022 0.0094 0.0003 0.0003 0.0002 0.0042 0.0009 0
Bud Neck 0.0003 0.0002 0.0003 0.0003 0.0005 0.0011 0.0006 0.0009 0.0006 0.0041 0.0134 0.0065 0.0016 0.0004 0.0001 0.0002 0.0056 0.0003
Bud Periphery 0.0001 0.0001 0.0003 0.0001 0.0002 0.0002 0.0057 0.0007 0.0073 0.0128 0.0228 0.0073 0.0003 0.0002 0.0001 0.0044 0.0007 0
Bud Site 0.0004 0.0028 0.0006 0.0002 0.0002 0.0001 0.0063 0.0189 0.0111 0.0032 0.0086 0.0018 0.0081 0.004 0.0006 0.0011 0.0026 0
Cell Periphery 0.0002 0.0001 0.0001 0.0001 0.0001 0.0001 0.0005 0.0001 0.0003 0.0003 0.0005 0.0002 0.0002 0.0001 0.0001 0.0002 0.0001 0
Cytoplasm 0.8198 0.9186 0.8657 0.8946 0.8396 0.9005 0.728 0.8932 0.8116 0.7975 0.6447 0.8542 0.7493 0.8694 0.8425 0.8061 0.6435 0.9194
Cytoplasmic Foci 0.0135 0.0085 0.0137 0.0177 0.0231 0.0086 0.0309 0.0078 0.0195 0.0219 0.0613 0.0206 0.0189 0.0175 0.0205 0.0093 0.0335 0.0054
Eisosomes 0.0001 0 0.0001 0.0001 0.0002 0 0.0003 0 0.0001 0 0.0002 0 0.0002 0 0 0.0003 0 0
Endoplasmic Reticulum 0.0099 0.0017 0.0052 0.0045 0.0057 0.0033 0.006 0.0035 0.0018 0.0024 0.0035 0.0015 0.0098 0.0054 0.0038 0.0045 0.0055 0.0023
Endosome 0.0156 0.0056 0.0225 0.0267 0.0149 0.0113 0.0238 0.003 0.012 0.0261 0.0271 0.0096 0.0365 0.0126 0.0137 0.0112 0.1125 0.006
Golgi 0.0026 0.0005 0.0018 0.0016 0.0045 0.0007 0.0104 0.0003 0.0044 0.0042 0.0117 0.0025 0.0054 0.0057 0.0095 0.0039 0.0592 0.0002
Lipid Particles 0.0017 0.0001 0.001 0.001 0.007 0.0002 0.0126 0.0001 0.0021 0.0006 0.0069 0.0049 0.0056 0.0016 0.0071 0.0008 0.008 0.0002
Mitochondria 0.0005 0.0002 0.0053 0.0005 0.001 0.0003 0.0057 0.0002 0.0072 0.0069 0.0219 0.0029 0.002 0.0009 0.005 0.0514 0.0066 0.0001
None 0.1014 0.0569 0.0668 0.0432 0.0359 0.0656 0.0993 0.0648 0.0726 0.0699 0.0818 0.0605 0.1129 0.0727 0.0793 0.0605 0.0994 0.0644
Nuclear Periphery 0.0022 0.0004 0.0016 0.001 0.0194 0.0007 0.0035 0.0004 0.0029 0.0016 0.0021 0.001 0.008 0.0015 0.0011 0.0022 0.001 0.0003
Nucleolus 0.0001 0 0.0001 0.0001 0.0002 0 0.0004 0.0001 0.0006 0.0002 0.0006 0.0002 0.0013 0 0.0001 0.0001 0.0002 0
Nucleus 0.0018 0.001 0.0011 0.0008 0.0017 0.0007 0.0023 0.0013 0.0058 0.003 0.0023 0.0021 0.0124 0.001 0.0009 0.0013 0.0007 0.0004
Peroxisomes 0.0038 0.0001 0.0009 0.0005 0.0128 0.0001 0.0188 0.0002 0.0078 0.0033 0.0407 0.0027 0.0037 0.002 0.0084 0.0008 0.0067 0
Punctate Nuclear 0.0011 0.0002 0.0006 0.0005 0.004 0.0002 0.003 0.0008 0.0027 0.001 0.0031 0.0021 0.0045 0.0005 0.0023 0.0006 0.0009 0.0001
Vacuole 0.0021 0.0019 0.0019 0.0041 0.0021 0.001 0.0029 0.0012 0.0025 0.0186 0.0031 0.0012 0.0024 0.0014 0.0015 0.0051 0.0036 0.0006
Vacuole Periphery 0.0004 0.0002 0.0005 0.0006 0.001 0.0003 0.001 0.0002 0.0008 0.0025 0.0012 0.0003 0.0008 0.0002 0.0004 0.0013 0.0014 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 8.7695 12.4508 8.8968 15.2242 9.6979 8.249 14.6871 19.5109 16.8373 15.1387
Translational Efficiency 0.5771 0.6134 0.7468 0.6181 0.6886 1.038 0.6113 0.5136 0.617 0.6126

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘
Standard name
Human Ortholog
Description Cytoplasmic phosphorelay intermediate osmosensor and regulator; part of a two-component signal transducer that mediates osmosensing via a phosphorelay mechanism; required for mitophagy; dephosphorylated form is degraded by the ubiquitin-proteasome system; potential Cdc28p substrate
Localization
Cell Percentages cytoplasm (96%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Ssk1

Ssk1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ssk1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available