Standard name
Human Ortholog
Description Guanine nucleotide exchange factor (GEF) for Ria1p; essential protein involved in ribosome maturation; with Ria1p, promotes release of Tif6p from 60S ribosomal subunits in the cytoplasm so that they can assemble with 40S subunits to generate mature ribosomes; ortholog of the human protein (SBDS) responsible for autosomal recessive Shwachman-Bodian-Diamond Syndrome; highly conserved across archaea and eukaryotes
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0.11 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.97 0.99 0.99 0.98 0.96 0.93 0.91 – 0.78 0.73 0.8 0.64 0.99 0.99 1.0 0.98 0.94 0.95 0.92 0.96 0.96 0.93 0.97 0.94
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0.1 – 0.2 0.26 0.19 0.3 0 0 0 0 0.05 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 – 0 0 0.05 0.05 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 – 0.07 0.07 0.07 0.1 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 1 0 – 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 1
Bud 0 1 0 0 0 0 3 – 0 1 0 1 0 0 0 0 0 0 0 0 1 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 5 5 5 12 3 1 – 0 5 2 4 4 4 8 3 8 9 0 0 0 0 0 0
Cytoplasm 127 405 195 149 109 186 197 – 166 166 153 98 171 220 292 234 226 240 129 390 184 120 193 167
Endoplasmic Reticulum 1 3 2 2 1 3 2 – 3 0 1 1 2 2 0 11 5 8 0 2 1 0 0 1
Endosome 0 0 0 1 0 0 0 – 1 0 0 0 0 0 0 1 1 2 0 2 0 1 0 0
Golgi 0 1 0 0 0 0 0 – 0 0 0 0 0 0 0 0 2 2 0 1 0 0 0 0
Mitochondria 1 1 0 0 1 3 21 – 42 59 36 46 0 0 0 6 11 3 0 0 0 0 0 1
Nucleus 5 0 1 0 1 1 6 – 8 10 9 7 1 2 0 2 5 10 1 3 1 1 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 1 0 0 0 0 0 0 0 0 0 0 0 0 1 0 1
Nucleolus 0 0 0 0 0 1 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 2 5 2 – 15 17 13 15 0 0 0 4 6 5 4 3 0 1 1 1
Unique Cell Count 131 410 197 152 113 199 217 213 228 192 154 173 222 293 240 240 253 140 408 193 129 200 179
Labelled Cell Count 134 416 203 157 126 203 232 236 258 215 172 178 228 300 262 264 279 140 408 193 129 200 179


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 10.3 10.5 11.5 10.2 9.8 7.8 4.5 – 6.2 6.0 6.1 6.1 11.1 10.5 10.0 8.8 10.9 11.6 11.8 12.1 12.1
Std Deviation (1e-4) 2.5 1.8 1.7 1.3 1.6 1.5 1.1 – 1.2 1.1 1.1 1.2 1.6 1.4 1.4 1.7 2.2 2.7 1.9 1.8 2.0
Intensity Change (Log2) – – – -0.18 -0.23 -0.56 -1.35 – -0.9 -0.93 -0.91 -0.91 -0.05 -0.13 -0.2 -0.38 -0.07 0.01 0.04 0.07 0.08


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 3.0 0 0 – 0 0 0 0 0 0 0.1 0 0.5 0.6
Cytoplasm -0.7 -1.6 -2.9 -3.7 – -6.6 -7.5 -6.2 -8.8 -0.1 0.1 0.9 -1.2 -2.7 -2.4
Endoplasmic Reticulum 0 0 0 0 – 0 0 0 0 0 0 0 2.2 0 0
Endosome 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 – 6.6 7.7 6.4 8.2 0 0 0 0 0 0
Nucleus 0 0 0 0 – 0 2.5 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 – 3.8 3.9 3.7 4.5 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 3.9807 4.5678 4.6803 4.2453 4.3808 4.6998 7.8527 9.5259 9.3914 8.0972 10.0957 8.99 5.4413 7.2529 6.4564 6.8603 6.1207 6.4708
Actin 0.0164 0.0005 0.024 0.0089 0.0001 0.0016 0.0486 0.0003 0.0086 0.0094 0.0005 0.0078 0.0178 0.0043 0.0184 0.0103 0.0268 0.0034
Bud 0.0043 0.0014 0.0009 0.0045 0.0001 0.0003 0.001 0.0001 0.0027 0.0021 0.0001 0.0003 0.0006 0.0002 0.0005 0.0009 0.0007 0.0001
Bud Neck 0.0086 0.0002 0.0005 0.0012 0.0005 0.0009 0.0009 0.0001 0.0006 0.0011 0.0006 0.0012 0.0008 0.0001 0.0007 0.0033 0.0009 0.0005
Bud Periphery 0.003 0.0006 0.0013 0.0014 0.0001 0.0002 0.001 0 0.0015 0.0013 0 0.0004 0.0006 0.0001 0.0003 0.001 0.0003 0.0001
Bud Site 0.0055 0.0029 0.0018 0.0049 0.0001 0.0002 0.0021 0.0005 0.0103 0.0082 0.0001 0.0002 0.0019 0.0047 0.0051 0.0021 0.0074 0.0002
Cell Periphery 0.0002 0.0001 0.0002 0.0002 0 0 0.0008 0.0001 0.0004 0.0005 0 0 0.0003 0.0001 0.0002 0.0002 0.0001 0
Cytoplasm 0.7727 0.9008 0.8369 0.8889 0.9197 0.9339 0.6116 0.9603 0.8617 0.8655 0.8791 0.91 0.7399 0.9626 0.9051 0.7886 0.8505 0.9321
Cytoplasmic Foci 0.0306 0.0099 0.0169 0.0184 0.0115 0.0122 0.0398 0.0103 0.0117 0.0169 0.0038 0.0113 0.0272 0.0026 0.0114 0.0352 0.0181 0.007
Eisosomes 0.0001 0 0.0002 0.0001 0 0 0.0005 0 0.0001 0.0001 0 0.0001 0.0002 0 0.0001 0.0001 0.0001 0
Endoplasmic Reticulum 0.0095 0.0034 0.0022 0.0026 0.0012 0.0055 0.012 0.0038 0.006 0.0058 0.0026 0.0021 0.0164 0.0016 0.0034 0.0051 0.0025 0.0028
Endosome 0.042 0.0099 0.0223 0.0191 0.0152 0.0128 0.0474 0.0041 0.0244 0.0279 0.0043 0.0083 0.0515 0.0008 0.0184 0.0569 0.0246 0.0133
Golgi 0.0043 0.0005 0.0068 0.0035 0.0024 0.0055 0.0243 0.0005 0.0041 0.0071 0.0001 0.0051 0.0173 0.0006 0.0043 0.0352 0.0317 0.0084
Lipid Particles 0.0029 0.0001 0.0021 0.0033 0 0.0014 0.018 0.0108 0.0014 0.0005 0 0.0005 0.0119 0.0001 0.0029 0.0047 0.013 0.0016
Mitochondria 0.0049 0.0004 0.0185 0.0012 0.0358 0.002 0.0104 0.0001 0.0044 0.009 0.0003 0.0182 0.0157 0.0004 0.003 0.014 0.0021 0.0102
None 0.0497 0.0592 0.0528 0.0262 0.0053 0.0172 0.0947 0.0057 0.0421 0.0037 0.0047 0.0307 0.0421 0.0202 0.0096 0.0125 0.0106 0.0139
Nuclear Periphery 0.0164 0.0014 0.0013 0.0015 0.0006 0.0011 0.0183 0.0007 0.0064 0.0017 0.0438 0.0006 0.0214 0.0002 0.0036 0.0071 0.0005 0.0004
Nucleolus 0.0006 0.0001 0.0001 0.0002 0 0.0001 0.0014 0.0001 0.0003 0.0001 0.0002 0 0.0011 0 0.0004 0.0003 0 0
Nucleus 0.0118 0.0037 0.0022 0.0039 0.0026 0.0016 0.0231 0.0011 0.0069 0.0027 0.0544 0.0011 0.0146 0.0005 0.0066 0.002 0.0012 0.0007
Peroxisomes 0.0036 0.0001 0.0018 0.0027 0.0003 0.0007 0.0226 0.0002 0.0011 0.0014 0 0.0003 0.0055 0.0005 0.0033 0.0067 0.0069 0.0039
Punctate Nuclear 0.0041 0.0006 0.0016 0.0022 0.0004 0.0006 0.0153 0.0006 0.0019 0.0005 0.0038 0.0008 0.0052 0.0001 0.0004 0.0038 0.0007 0.0002
Vacuole 0.0062 0.0036 0.0039 0.0042 0.0034 0.0017 0.0045 0.0006 0.0026 0.0332 0.0008 0.0006 0.0052 0.0003 0.0015 0.0056 0.0009 0.0008
Vacuole Periphery 0.0028 0.0005 0.0018 0.0009 0.0008 0.0004 0.0016 0.0001 0.0007 0.0014 0.0007 0.0004 0.0028 0 0.0005 0.0045 0.0003 0.0004

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 28.5742 23.5787 32.0768 34.426 40.8475 14.6453 27.1516 26.8178 27.764 36.7712
Translational Efficiency 2.4144 2.4152 1.7691 1.6305 1.6359 4.3436 1.4928 1.6864 1.5824 1.5234

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Guanine nucleotide exchange factor (GEF) for Ria1p; essential protein involved in ribosome maturation; with Ria1p, promotes release of Tif6p from 60S ribosomal subunits in the cytoplasm so that they can assemble with 40S subunits to generate mature ribosomes; ortholog of the human protein (SBDS) responsible for autosomal recessive Shwachman-Bodian-Diamond Syndrome; highly conserved across archaea and eukaryotes
Localization
Cell Percentages cytoplasm (98%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-4

Sdo1

Sdo1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Sdo1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available