Standard name | |
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Human Ortholog | |
Description | Cytoplasmic ubiquitin-protein ligase (E3); component of the Mub1p-Ubr2p-Rad6p ubiquitin ligase complex required for the ubiquitination and degradation of Rpn4p; mediates formation of the ternary complex |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | – | – | – | 2 | 1 | 2 | 0 | 0 | 4 |
Bud | 0 | 1 | 2 | 0 | 0 | 0 | 2 | 3 | 0 | 4 | 2 | 3 | 1 | 0 | 5 | – | – | – | 1 | 0 | 1 | 0 | 1 | 2 |
Bud Neck | 1 | 0 | 4 | 2 | 3 | 1 | 0 | 2 | 1 | 2 | 2 | 0 | 0 | 1 | 0 | – | – | – | 1 | 2 | 1 | 0 | 2 | 1 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | – | – | – | – | – | – | – | – | – |
Cell Periphery | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 1 | 0 | 0 | 0 | 1 | 0 | – | – | – | 1 | 0 | 1 | 0 | 1 | 1 |
Cytoplasm | 357 | 186 | 198 | 163 | 234 | 157 | 203 | 226 | 108 | 111 | 56 | 84 | 127 | 219 | 234 | – | – | – | 287 | 156 | 113 | 139 | 195 | 216 |
Endoplasmic Reticulum | 1 | 0 | 1 | 1 | 0 | 0 | 0 | 1 | 1 | 0 | 2 | 1 | 0 | 0 | 0 | – | – | – | 2 | 0 | 1 | 1 | 0 | 1 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | 6 | 2 | 2 | 0 | 1 | 2 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | 4 | 0 | 1 | 0 | 0 | 1 |
Mitochondria | 5 | 27 | 15 | 11 | 13 | 44 | 34 | 41 | 59 | 65 | 44 | 62 | 1 | 0 | 9 | – | – | – | 43 | 19 | 23 | 5 | 10 | 10 |
Nucleus | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 1 | 0 | 0 | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 1 | 1 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 | 0 | 0 | 1 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 2 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 1 | 0 | 0 | 0 | 4 | 4 | 4 | 3 | 3 | 3 | 2 | 3 | 0 | 0 | 0 | – | – | – | 7 | 2 | 1 | 0 | 1 | 1 |
Vac/Vac Membrane | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 1 | 1 | 0 | 2 | 0 | 0 | 0 | – | – | – | 4 | 4 | 2 | 1 | 2 | 8 |
Unique Cell Count | 362 | 195 | 209 | 165 | 249 | 185 | 225 | 250 | 147 | 156 | 92 | 123 | 129 | 220 | 237 | 372 | 194 | 167 | 157 | 223 | 257 | |||
Labelled Cell Count | 370 | 216 | 221 | 178 | 256 | 207 | 245 | 280 | 174 | 190 | 111 | 157 | 130 | 222 | 249 | 372 | 194 | 167 | 157 | 223 | 257 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 4.3 | 3.0 | 2.8 | 2.9 | 3.2 | 2.9 | 3.1 | 3.1 | 2.7 | 2.4 | 2.6 | 2.6 | 3.4 | 3.6 | 3.1 | – | – | – | 3.6 | 3.6 | 4.2 |
Std Deviation (1e-4) | 0.9 | 0.7 | 1.0 | 1.4 | 1.3 | 1.3 | 1.8 | 1.6 | 1.9 | 1.1 | 1.6 | 1.6 | 0.8 | 0.9 | 0.7 | – | – | – | 1.0 | 1.2 | 1.5 |
Intensity Change (Log2) | – | – | – | 0.07 | 0.18 | 0.06 | 0.13 | 0.13 | -0.06 | -0.22 | -0.09 | -0.09 | 0.29 | 0.38 | 0.15 | – | – | – | 0.36 | 0.38 | 0.56 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 0.467 | 1.1001 | 0.2943 | 0.493 | 0.677 | 0.8266 | -0.1818 | 0.6339 | 0.1501 | 0.1845 | 0.566 | 0.4785 | 1.2492 | 0.9892 | 1.2043 | 1.383 | 1.5842 | 1.6705 |
Actin | 0.0121 | 0.0001 | 0.0029 | 0.0001 | 0.053 | 0.0035 | 0.0067 | 0 | 0.0048 | 0.0002 | 0.0116 | 0.0072 | 0.0158 | 0.0019 | 0.0047 | 0.0231 | 0.0046 | 0.0015 |
Bud | 0.0004 | 0 | 0.0002 | 0 | 0.001 | 0.0004 | 0.001 | 0 | 0.002 | 0.0005 | 0.0001 | 0.0004 | 0.0002 | 0.0002 | 0.0035 | 0 | 0.0012 | 0.0004 |
Bud Neck | 0.0005 | 0 | 0.0002 | 0 | 0.0015 | 0.0077 | 0.0006 | 0 | 0.0003 | 0.0001 | 0.0006 | 0.0005 | 0.0012 | 0 | 0.0002 | 0.0002 | 0.0003 | 0.0003 |
Bud Periphery | 0.0012 | 0 | 0.0004 | 0 | 0.0021 | 0.001 | 0.0037 | 0 | 0.0017 | 0.001 | 0.0002 | 0.0023 | 0.0003 | 0.0007 | 0.0031 | 0 | 0.0023 | 0.0015 |
Bud Site | 0.0038 | 0.0001 | 0.0007 | 0 | 0.0299 | 0.0014 | 0.0013 | 0.0001 | 0.0039 | 0.0003 | 0.0005 | 0.0001 | 0.0013 | 0.0007 | 0.0062 | 0.0003 | 0.0038 | 0.0008 |
Cell Periphery | 0.0007 | 0.0002 | 0.0002 | 0.0001 | 0.0006 | 0.0002 | 0.0002 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.0002 | 0 | 0.0004 | 0 | 0.0001 | 0.0001 |
Cytoplasm | 0.0403 | 0.0356 | 0.0599 | 0.043 | 0.0316 | 0.0717 | 0.0129 | 0.0114 | 0.0122 | 0.0118 | 0.0062 | 0.0348 | 0.0164 | 0.0464 | 0.0234 | 0.0198 | 0.0153 | 0.0114 |
Cytoplasmic Foci | 0.0103 | 0.0009 | 0.0069 | 0.0015 | 0.0028 | 0.0036 | 0.013 | 0.0008 | 0.0048 | 0.0154 | 0.0403 | 0.0025 | 0.0082 | 0.0115 | 0.0083 | 0.0074 | 0.02 | 0.0159 |
Eisosomes | 0.0006 | 0.0011 | 0.0004 | 0.0003 | 0.0008 | 0.0002 | 0.0009 | 0.0002 | 0.0006 | 0.0002 | 0.0002 | 0.0002 | 0.0005 | 0.0003 | 0.0005 | 0.0005 | 0.0003 | 0.0001 |
Endoplasmic Reticulum | 0.0013 | 0.0004 | 0.0023 | 0.0003 | 0.0013 | 0.0022 | 0.0016 | 0.0002 | 0.0002 | 0.0002 | 0.0002 | 0.0006 | 0.0006 | 0.0002 | 0.0013 | 0.0002 | 0.0011 | 0.0007 |
Endosome | 0.007 | 0 | 0.0052 | 0.0001 | 0.0044 | 0.0086 | 0.0122 | 0 | 0.0013 | 0.0008 | 0.0324 | 0.003 | 0.0015 | 0.0003 | 0.002 | 0.0003 | 0.0095 | 0.0004 |
Golgi | 0.0024 | 0 | 0.0011 | 0 | 0.0028 | 0.004 | 0.0055 | 0 | 0.0047 | 0.0002 | 0.0384 | 0.0022 | 0.0015 | 0.0002 | 0.002 | 0.0011 | 0.0025 | 0.0001 |
Lipid Particles | 0.0123 | 0.0002 | 0.004 | 0.0001 | 0.0068 | 0.0054 | 0.0116 | 0.0001 | 0.0045 | 0.0011 | 0.0376 | 0.0015 | 0.0021 | 0.0005 | 0.0018 | 0.0028 | 0.0052 | 0.0005 |
Mitochondria | 0.0067 | 0 | 0.0066 | 0.0001 | 0.0695 | 0.0043 | 0.0021 | 0.0001 | 0.0085 | 0.0004 | 0.0151 | 0.0029 | 0.0005 | 0.0006 | 0.0022 | 0.0003 | 0.0092 | 0.0004 |
None | 0.8834 | 0.9609 | 0.9014 | 0.9531 | 0.7545 | 0.8584 | 0.9026 | 0.9859 | 0.9348 | 0.9632 | 0.7727 | 0.9352 | 0.9396 | 0.9256 | 0.9352 | 0.9225 | 0.8971 | 0.9621 |
Nuclear Periphery | 0.0009 | 0.0001 | 0.0023 | 0.0002 | 0.0044 | 0.0098 | 0.0009 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0.0007 | 0.0002 | 0.0001 | 0.0004 | 0 | 0.0036 | 0.0013 |
Nucleolus | 0.0007 | 0.0001 | 0.0004 | 0.0001 | 0.0063 | 0.0008 | 0.0017 | 0.0001 | 0.0002 | 0.0004 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0005 | 0 | 0.0008 | 0.0001 |
Nucleus | 0.0008 | 0.0001 | 0.0009 | 0.0003 | 0.0075 | 0.0051 | 0.0008 | 0.0001 | 0.0002 | 0.0003 | 0.0001 | 0.0003 | 0.0002 | 0.0001 | 0.0006 | 0 | 0.0017 | 0.0001 |
Peroxisomes | 0.0087 | 0 | 0.0019 | 0 | 0.0133 | 0.0046 | 0.016 | 0 | 0.0143 | 0.0008 | 0.0422 | 0.0045 | 0.0083 | 0.0093 | 0.0012 | 0.0211 | 0.0043 | 0.0015 |
Punctate Nuclear | 0.0011 | 0.0001 | 0.0009 | 0.0003 | 0.0022 | 0.002 | 0.0024 | 0.0007 | 0.0005 | 0.0022 | 0.0005 | 0.0005 | 0.0012 | 0.0012 | 0.0017 | 0.0002 | 0.0144 | 0.0004 |
Vacuole | 0.0037 | 0.0001 | 0.0007 | 0.0002 | 0.0031 | 0.0027 | 0.0018 | 0.0001 | 0.0001 | 0.0005 | 0.0005 | 0.0004 | 0.0002 | 0.0001 | 0.0004 | 0 | 0.0012 | 0.0002 |
Vacuole Periphery | 0.0011 | 0 | 0.0006 | 0 | 0.001 | 0.0024 | 0.0005 | 0 | 0.0001 | 0.0001 | 0.0004 | 0.0003 | 0 | 0 | 0.0001 | 0 | 0.0013 | 0.0002 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 12.0768 | 7.7239 | 10.6814 | 13.9373 | 9.6749 | 12.9533 | 15.2643 | 16.5317 | 15.2578 | 17.7167 |
Translational Efficiency | 0.3811 | 0.2833 | 0.3255 | 0.2182 | 0.282 | 0.3755 | 0.2572 | 0.1539 | 0.2584 | 0.2173 |
Dataset | Hit |
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Protein Concentration | ✔ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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