Standard name
Human Ortholog
Description Cytoplasmic ubiquitin-protein ligase (E3); component of the Mub1p-Ubr2p-Rad6p ubiquitin ligase complex required for the ubiquitination and degradation of Rpn4p; mediates formation of the ternary complex

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.99 0.95 0.95 0.99 0.94 0.85 0.9 0.9 0.73 0.71 0.61 0.68 0.98 1.0 0.99 0.77 0.81 0.68 0.89 0.87 0.84
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0.14 0.07 0.07 0.05 0.24 0.15 0.16 0.4 0.42 0.48 0.5 0 0 0 0.12 0.1 0.14 0 0.05 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 2 1 2 0 0 4
Bud 0 1 2 0 0 0 2 3 0 4 2 3 1 0 5 1 0 1 0 1 2
Bud Neck 1 0 4 2 3 1 0 2 1 2 2 0 0 1 0 1 2 1 0 2 1
Bud Site 0 0 0 0 0 0 0 0 0 1 1 0 0 0 0
Cell Periphery 3 0 0 0 0 0 0 2 1 1 0 0 0 1 0 1 0 1 0 1 1
Cytoplasm 357 186 198 163 234 157 203 226 108 111 56 84 127 219 234 287 156 113 139 195 216
Endoplasmic Reticulum 1 0 1 1 0 0 0 1 1 0 2 1 0 0 0 2 0 1 1 0 1
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 6 2 2 0 1 2
Golgi 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 4 0 1 0 0 1
Mitochondria 5 27 15 11 13 44 34 41 59 65 44 62 1 0 9 43 19 23 5 10 10
Nucleus 1 1 0 0 0 0 1 1 0 0 0 0 0 1 1 0 0 0 0 0 0
Nuclear Periphery 0 0 0 1 0 0 0 0 0 1 1 0 1 0 0 0 0 0 0 0 0
Nucleolus 0 1 1 0 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Peroxisomes 0 0 0 0 0 0 0 0 0 1 0 2 0 0 0 0 0 0 0 0 0
SpindlePole 1 0 0 0 4 4 4 3 3 3 2 3 0 0 0 7 2 1 0 1 1
Vac/Vac Membrane 1 0 0 0 1 0 0 1 1 1 0 2 0 0 0 4 4 2 1 2 8
Unique Cell Count 362 195 209 165 249 185 225 250 147 156 92 123 129 220 237 372 194 167 157 223 257
Labelled Cell Count 370 216 221 178 256 207 245 280 174 190 111 157 130 222 249 372 194 167 157 223 257


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.3 3.0 2.8 2.9 3.2 2.9 3.1 3.1 2.7 2.4 2.6 2.6 3.4 3.6 3.1 3.6 3.6 4.2
Std Deviation (1e-4) 0.9 0.7 1.0 1.4 1.3 1.3 1.8 1.6 1.9 1.1 1.6 1.6 0.8 0.9 0.7 1.0 1.2 1.5
Intensity Change (Log2) 0.07 0.18 0.06 0.13 0.13 -0.06 -0.22 -0.09 -0.09 0.29 0.38 0.15 0.36 0.38 0.56


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin
Bud
Bud Neck
Bud Site
Cell Periphery
Cyto
Endoplasmic Reticulum
Endosome
Golgi
Mitochondria
Nuclear Periphery
Nuc
Nucleolus
Peroxisomes
SpindlePole
Vac
Cortical Patches
Cytoplasm
Nucleus
Vacuole

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.467 1.1001 0.2943 0.493 0.677 0.8266 -0.1818 0.6339 0.1501 0.1845 0.566 0.4785 1.2492 0.9892 1.2043 1.383 1.5842 1.6705
Actin 0.0121 0.0001 0.0029 0.0001 0.053 0.0035 0.0067 0 0.0048 0.0002 0.0116 0.0072 0.0158 0.0019 0.0047 0.0231 0.0046 0.0015
Bud 0.0004 0 0.0002 0 0.001 0.0004 0.001 0 0.002 0.0005 0.0001 0.0004 0.0002 0.0002 0.0035 0 0.0012 0.0004
Bud Neck 0.0005 0 0.0002 0 0.0015 0.0077 0.0006 0 0.0003 0.0001 0.0006 0.0005 0.0012 0 0.0002 0.0002 0.0003 0.0003
Bud Periphery 0.0012 0 0.0004 0 0.0021 0.001 0.0037 0 0.0017 0.001 0.0002 0.0023 0.0003 0.0007 0.0031 0 0.0023 0.0015
Bud Site 0.0038 0.0001 0.0007 0 0.0299 0.0014 0.0013 0.0001 0.0039 0.0003 0.0005 0.0001 0.0013 0.0007 0.0062 0.0003 0.0038 0.0008
Cell Periphery 0.0007 0.0002 0.0002 0.0001 0.0006 0.0002 0.0002 0.0001 0.0001 0.0002 0.0001 0.0001 0.0002 0 0.0004 0 0.0001 0.0001
Cytoplasm 0.0403 0.0356 0.0599 0.043 0.0316 0.0717 0.0129 0.0114 0.0122 0.0118 0.0062 0.0348 0.0164 0.0464 0.0234 0.0198 0.0153 0.0114
Cytoplasmic Foci 0.0103 0.0009 0.0069 0.0015 0.0028 0.0036 0.013 0.0008 0.0048 0.0154 0.0403 0.0025 0.0082 0.0115 0.0083 0.0074 0.02 0.0159
Eisosomes 0.0006 0.0011 0.0004 0.0003 0.0008 0.0002 0.0009 0.0002 0.0006 0.0002 0.0002 0.0002 0.0005 0.0003 0.0005 0.0005 0.0003 0.0001
Endoplasmic Reticulum 0.0013 0.0004 0.0023 0.0003 0.0013 0.0022 0.0016 0.0002 0.0002 0.0002 0.0002 0.0006 0.0006 0.0002 0.0013 0.0002 0.0011 0.0007
Endosome 0.007 0 0.0052 0.0001 0.0044 0.0086 0.0122 0 0.0013 0.0008 0.0324 0.003 0.0015 0.0003 0.002 0.0003 0.0095 0.0004
Golgi 0.0024 0 0.0011 0 0.0028 0.004 0.0055 0 0.0047 0.0002 0.0384 0.0022 0.0015 0.0002 0.002 0.0011 0.0025 0.0001
Lipid Particles 0.0123 0.0002 0.004 0.0001 0.0068 0.0054 0.0116 0.0001 0.0045 0.0011 0.0376 0.0015 0.0021 0.0005 0.0018 0.0028 0.0052 0.0005
Mitochondria 0.0067 0 0.0066 0.0001 0.0695 0.0043 0.0021 0.0001 0.0085 0.0004 0.0151 0.0029 0.0005 0.0006 0.0022 0.0003 0.0092 0.0004
None 0.8834 0.9609 0.9014 0.9531 0.7545 0.8584 0.9026 0.9859 0.9348 0.9632 0.7727 0.9352 0.9396 0.9256 0.9352 0.9225 0.8971 0.9621
Nuclear Periphery 0.0009 0.0001 0.0023 0.0002 0.0044 0.0098 0.0009 0.0001 0.0001 0.0002 0.0001 0.0007 0.0002 0.0001 0.0004 0 0.0036 0.0013
Nucleolus 0.0007 0.0001 0.0004 0.0001 0.0063 0.0008 0.0017 0.0001 0.0002 0.0004 0.0001 0.0001 0.0001 0.0001 0.0005 0 0.0008 0.0001
Nucleus 0.0008 0.0001 0.0009 0.0003 0.0075 0.0051 0.0008 0.0001 0.0002 0.0003 0.0001 0.0003 0.0002 0.0001 0.0006 0 0.0017 0.0001
Peroxisomes 0.0087 0 0.0019 0 0.0133 0.0046 0.016 0 0.0143 0.0008 0.0422 0.0045 0.0083 0.0093 0.0012 0.0211 0.0043 0.0015
Punctate Nuclear 0.0011 0.0001 0.0009 0.0003 0.0022 0.002 0.0024 0.0007 0.0005 0.0022 0.0005 0.0005 0.0012 0.0012 0.0017 0.0002 0.0144 0.0004
Vacuole 0.0037 0.0001 0.0007 0.0002 0.0031 0.0027 0.0018 0.0001 0.0001 0.0005 0.0005 0.0004 0.0002 0.0001 0.0004 0 0.0012 0.0002
Vacuole Periphery 0.0011 0 0.0006 0 0.001 0.0024 0.0005 0 0.0001 0.0001 0.0004 0.0003 0 0 0.0001 0 0.0013 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 12.0768 7.7239 10.6814 13.9373 9.6749 12.9533 15.2643 16.5317 15.2578 17.7167
Translational Efficiency 0.3811 0.2833 0.3255 0.2182 0.282 0.3755 0.2572 0.1539 0.2584 0.2173

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency
Standard name
Human Ortholog
Description Cytoplasmic ubiquitin-protein ligase (E3); component of the Mub1p-Ubr2p-Rad6p ubiquitin ligase complex required for the ubiquitination and degradation of Rpn4p; mediates formation of the ternary complex
Localization
Cell Percentages nucleus (14%), cytoplasm (13%), mixed (51%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Ubr2

Ubr2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ubr2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available