Standard name
Human Ortholog
Description Putative divalent metal ion transporter involved in iron homeostasis; transcriptionally regulated by metal ions; member of the Nramp family of metal transport proteins; protein abundance increases in response to DNA replication stress
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0.2 0.14 0.06 0 0 0 0 0 0 0 0 0
Cytoplasm 0.57 0.68 0.63 0.92 0.73 0.69 0.64 0.75 0.81 0.76 0.82 0.88 0.17 0.4 0.75 0.4 0.37 0.36 0 0 0 0.06 0.07 0.07
Endoplasmic Reticulum 0.09 0.27 0.21 0.05 0 0.05 0 0 0 0 0 0 0.73 0.5 0.2 0 0 0 0.45 0.44 0.39 0.49 0.45 0.49
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0.07 0.06 0.08 0.09 0 0.07 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0.05 0.05 0 0 0 0 0 0 0 0.09 0.13 0.1 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.27 0.16 0.2 0.05 0.41 0.36 0.49 0.29 0.31 0.36 0.24 0.16 0 0.09 0.05 0.63 0.67 0.65 0.45 0.46 0.5 0.38 0.41 0.33
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 23 9 1 0 0 0 0 0 2 1 2 2
Bud 0 0 0 0 0 0 1 1 0 0 1 0 1 1 0 0 0 0 0 1 0 2 1 4
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 2 0 0 0 0 1 0 0 0 0 0 0 66 51 29 0 0 0 0 0 0 0 0 0
Cytoplasm 51 102 51 56 64 120 131 210 147 124 169 168 56 146 336 135 96 97 6 7 0 14 15 14
Endoplasmic Reticulum 8 40 17 3 1 8 5 3 3 2 1 4 243 183 92 13 9 2 61 81 43 118 103 101
Endosome 4 0 0 0 0 3 0 0 0 0 0 0 1 5 1 19 9 8 1 2 2 1 3 4
Golgi 0 0 0 1 0 0 0 0 0 0 0 0 4 1 0 1 1 2 1 0 2 2 0 1
Mitochondria 4 0 3 0 2 2 14 16 14 14 8 14 0 0 0 5 1 3 0 0 1 5 3 4
Nucleus 1 0 1 0 0 2 10 14 7 4 2 0 1 0 1 31 34 27 0 1 0 0 0 0
Nuclear Periphery 0 1 0 0 0 0 1 1 1 2 2 0 3 0 0 0 0 1 1 0 0 0 0 0
Nucleolus 2 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 1 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 1 0 0 0 0 0 0 0
Vac/Vac Membrane 24 24 16 3 36 62 101 82 56 59 50 30 5 34 21 213 176 176 61 84 55 91 93 67
Unique Cell Count 90 150 81 61 88 174 205 281 182 163 206 190 331 366 451 337 261 272 137 183 110 241 230 205
Labelled Cell Count 96 167 88 64 103 198 263 327 228 205 233 216 404 431 481 417 327 316 137 183 110 241 230 205


Endoplasmic Reticulum

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 7.7 9.2 7.2 8.3 8.0 6.8 5.8 5.8 5.7 5.2 5.3 5.0 15.5 10.3 7.4 7.7 9.1 8.9 7.2 7.3 8.6
Std Deviation (1e-4) 1.5 1.8 1.6 1.9 1.9 1.9 1.7 1.6 1.8 1.8 1.4 1.3 4.5 2.4 1.7 1.8 1.9 2.1 1.6 1.7 1.7
Intensity Change (Log2) – – – 0.2 0.16 -0.07 -0.31 -0.3 -0.33 -0.48 -0.44 -0.51 1.11 0.52 0.04 0.09 0.33 0.31 0.0 0.03 0.26


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 4.4 3.6 0 0 0 0
Cytoplasm 3.9 1.4 0.9 0.1 2.1 3.1 2.1 3.4 4.9 -8.5 -3.8 2.1 -3.7 -4.2 -4.4
Endoplasmic Reticulum -2.7 -4.2 -4.1 -5.3 0 -5.5 -5.4 -6.4 -5.3 8.8 4.8 -0.1 -5.4 -5.2 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 1.2 1.4 0 1.1 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 2.4 3.1 2.5
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole -2.6 3.0 2.6 4.6 1.7 1.8 2.6 0.8 -0.8 0 -2.7 -4.9 7.1 7.6 7.1

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 7.3828 9.7039 8.8004 8.5327 10.0092 8.9912 6.8142 8.4231 8.0806 8.2201 7.8838 8.0127 9.631 11.1724 11.2454 12.269 11.7887 11.1976
Actin 0.0119 0 0.0001 0.0005 0.0001 0.0001 0.04 0.0001 0.0058 0.0004 0.0011 0.0095 0.0003 0.0001 0.0149 0.0002 0 0.002
Bud 0.0001 0.0002 0.0002 0.0007 0.0007 0.0002 0.0002 0.0001 0.0007 0.0002 0.0002 0.0001 0.0004 0.0002 0.0005 0.0002 0.0001 0.0002
Bud Neck 0.0008 0.0009 0.0007 0.0017 0.0014 0.0021 0.0006 0.0008 0.0026 0.0011 0.0013 0.0012 0.0012 0.0009 0.0011 0.0006 0.0003 0.0017
Bud Periphery 0.0007 0.0008 0.0007 0.0028 0.0013 0.0019 0.0005 0.0004 0.0014 0.0004 0.0009 0.0007 0.0025 0.001 0.001 0.001 0.0002 0.0014
Bud Site 0.0004 0.0007 0.0007 0.0007 0.0014 0.0003 0.0008 0.0008 0.0077 0.0002 0.0005 0.0002 0.0007 0.0009 0.0103 0.0003 0.0001 0.0003
Cell Periphery 0.0049 0.0141 0.0092 0.0079 0.0072 0.0127 0.0022 0.0115 0.005 0.002 0.0062 0.0063 0.0206 0.0085 0.0064 0.0039 0.001 0.0147
Cytoplasm 0.0184 0.0565 0.0333 0.0746 0.0989 0.0273 0.084 0.0973 0.1204 0.1601 0.0436 0.0386 0.0263 0.0494 0.0557 0.0458 0.0641 0.0185
Cytoplasmic Foci 0.0049 0.002 0.0029 0.0009 0.0057 0.0012 0.0213 0.0075 0.0136 0.0087 0.0025 0.0024 0.0012 0.0051 0.0148 0.0047 0.0026 0.0054
Eisosomes 0.0001 0 0 0 0 0 0.0003 0 0.0001 0 0 0 0 0 0.0002 0 0 0
Endoplasmic Reticulum 0.51 0.4466 0.4959 0.5464 0.4348 0.4805 0.6498 0.5503 0.6158 0.5138 0.4326 0.5983 0.6626 0.4857 0.5227 0.5935 0.2229 0.4603
Endosome 0.0604 0.0382 0.0527 0.0141 0.0736 0.0351 0.0517 0.0655 0.0615 0.1051 0.0445 0.0475 0.0279 0.0534 0.047 0.0913 0.0625 0.0445
Golgi 0.004 0.0011 0.0013 0.0005 0.0017 0.0007 0.0071 0.0009 0.0161 0.0218 0.002 0.0028 0.0007 0.001 0.0109 0.0052 0.0019 0.0016
Lipid Particles 0.0164 0.0095 0.009 0.0045 0.0031 0.003 0.0095 0.0151 0.0188 0.0102 0.011 0.0054 0.0099 0.0209 0.0195 0.0042 0.0996 0.0091
Mitochondria 0.0017 0.0003 0.0008 0.0005 0.001 0.0008 0.0033 0.0002 0.0056 0.0013 0.0007 0.002 0.0013 0.0004 0.0026 0.0015 0.002 0.0009
None 0.0074 0.0003 0.0004 0.0001 0.0004 0.0001 0.0029 0.0054 0.0021 0.0024 0.0005 0.0004 0.0005 0.0011 0.0022 0.0002 0.178 0.0006
Nuclear Periphery 0.0786 0.0329 0.04 0.0397 0.0265 0.0494 0.0366 0.0191 0.0161 0.0218 0.0392 0.0294 0.0674 0.048 0.0359 0.0337 0.0433 0.0506
Nucleolus 0.0006 0.0004 0.0006 0.0001 0.0078 0.0005 0.0002 0.0003 0.0009 0 0.0003 0.0001 0.0024 0.0004 0.0003 0.0001 0.0042 0.0004
Nucleus 0.0077 0.0055 0.0054 0.0035 0.0334 0.0122 0.0055 0.0026 0.003 0.0015 0.0064 0.0036 0.0128 0.0038 0.0042 0.0022 0.003 0.0061
Peroxisomes 0.0012 0 0 0 0.0001 0 0.0019 0.0001 0.0016 0.0001 0.0001 0.0014 0.0001 0.0001 0.0076 0.0001 0.0004 0.0008
Punctate Nuclear 0.001 0.0002 0.0003 0.0001 0.0004 0.0001 0.0022 0.0005 0.002 0.0002 0.0003 0.0002 0.0012 0.0003 0.0014 0.0002 0.0001 0.0032
Vacuole 0.2528 0.3786 0.3282 0.2845 0.2796 0.3468 0.0743 0.2158 0.0961 0.1363 0.3864 0.2343 0.1464 0.3014 0.2165 0.1998 0.2349 0.3509
Vacuole Periphery 0.0161 0.0113 0.0177 0.0161 0.0211 0.0247 0.0048 0.0057 0.003 0.0124 0.0196 0.0153 0.0137 0.0173 0.0244 0.0115 0.0789 0.0269

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 50.0489 80.4639 87.5026 81.4939 32.1938 76.0012 129.575 136.5661 107.9797 98.1421
Translational Efficiency 1.0739 1.1628 1.0509 0.8279 0.9264 0.9006 1.0465 0.8586 1.0685 0.8211

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘
Standard name
Human Ortholog
Description Putative divalent metal ion transporter involved in iron homeostasis; transcriptionally regulated by metal ions; member of the Nramp family of metal transport proteins; protein abundance increases in response to DNA replication stress
Localization
Cell Percentages ER (47%), vacuole (12%), mixed (40%)
Cell Cycle Regulation Yes
vacuole - MA (0.00055)
Subcompartmental Group N/A

Smf3

Smf3


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Smf3-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available