Standard name | |
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Human Ortholog | |
Description | Protein involved in mismatch repair and meiotic recombination; only certain frameshift intermediates are mismatch repair substrates; forms a complex with Mlh1p |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 2 | 0 | 2 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 2 | 0 | 1 | 4 |
Bud | 1 | 0 | 0 | 0 | 1 | 4 | 3 | – | 14 | 10 | 17 | 3 | 2 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 3 | 4 | 6 | 12 |
Bud Neck | 0 | 0 | 13 | 4 | 23 | 12 | 17 | – | 7 | 3 | 3 | 12 | 0 | 0 | 1 | 3 | 0 | 0 | 0 | 0 | 2 | 3 | 4 | 10 |
Bud Site | 0 | 0 | 0 | 0 | 1 | 1 | 1 | – | 9 | 3 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 1 | 0 | 1 | 1 | 2 | – | 0 | 0 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 1 |
Cytoplasm | 100 | 71 | 262 | 270 | 448 | 459 | 555 | – | 368 | 440 | 324 | 329 | 158 | 199 | 312 | 294 | 145 | 137 | 77 | 68 | 177 | 101 | 193 | 246 |
Endoplasmic Reticulum | 1 | 2 | 2 | 2 | 0 | 0 | 2 | – | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 32 | 11 | 16 | 0 | 0 | 0 | 2 | 6 | 6 |
Endosome | 1 | 0 | 0 | 1 | 5 | 3 | 2 | – | 2 | 2 | 0 | 5 | 2 | 6 | 1 | 13 | 5 | 8 | 2 | 0 | 3 | 1 | 4 | 4 |
Golgi | 0 | 0 | 0 | 0 | 1 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 1 | 0 | 2 | 0 |
Mitochondria | 53 | 30 | 3 | 25 | 31 | 180 | 222 | – | 427 | 368 | 401 | 204 | 92 | 31 | 28 | 12 | 5 | 6 | 17 | 10 | 34 | 19 | 46 | 51 |
Nucleus | 16 | 24 | 37 | 54 | 65 | 69 | 88 | – | 97 | 75 | 61 | 54 | 100 | 83 | 72 | 35 | 21 | 11 | 48 | 51 | 67 | 45 | 44 | 25 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 1 | – | 2 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 1 | 0 | 0 | 0 | 0 | 0 | 1 | – | 1 | 1 | 1 | 3 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 1 | 3 | 10 | 6 | 5 | – | 5 | 11 | 3 | 21 | 0 | 0 | 1 | 0 | 3 | 0 | 9 | 3 | 12 | 6 | 13 | 18 |
Vac/Vac Membrane | 50 | 49 | 36 | 33 | 71 | 60 | 36 | – | 32 | 35 | 36 | 40 | 73 | 148 | 125 | 133 | 81 | 73 | 65 | 61 | 157 | 144 | 160 | 170 |
Unique Cell Count | 186 | 156 | 330 | 374 | 611 | 703 | 825 | 779 | 814 | 718 | 592 | 375 | 425 | 509 | 440 | 239 | 215 | 231 | 204 | 479 | 336 | 496 | 565 | |
Labelled Cell Count | 223 | 176 | 357 | 392 | 659 | 795 | 935 | 964 | 949 | 850 | 673 | 429 | 467 | 543 | 522 | 272 | 255 | 231 | 204 | 479 | 336 | 496 | 565 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 3.7 | 3.9 | 4.9 | 4.3 | 5.0 | 3.8 | 3.9 | – | 3.4 | 3.5 | 3.3 | 3.9 | 3.7 | 4.0 | 3.9 | 5.6 | 6.0 | 5.6 | 3.6 | 3.7 | 4.1 |
Std Deviation (1e-4) | 0.5 | 0.7 | 0.8 | 1.0 | 1.2 | 0.9 | 1.1 | – | 0.7 | 0.8 | 0.7 | 0.7 | 0.8 | 0.8 | 0.7 | 1.1 | 1.2 | 1.0 | 0.7 | 1.2 | 1.3 |
Intensity Change (Log2) | – | – | – | -0.17 | 0.02 | -0.38 | -0.31 | – | -0.54 | -0.49 | -0.57 | -0.35 | -0.39 | -0.31 | -0.34 | 0.19 | 0.28 | 0.2 | -0.45 | -0.41 | -0.25 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | 6.4 | 6.7 | 6.6 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 0.1487 | -0.5801 | -0.6111 | 0.179 | -0.6333 | -0.4359 | -0.4791 | -0.9238 | -0.6641 | -0.6838 | -1.1166 | -0.7413 | -0.3288 | -0.63 | -0.9922 | -0.9841 | -0.968 | -0.802 |
Actin | 0.0478 | 0.0011 | 0.0037 | 0.0181 | 0.0034 | 0.0061 | 0.0152 | 0.0003 | 0.015 | 0.0018 | 0.0004 | 0.0034 | 0.0662 | 0.0003 | 0.0039 | 0.0005 | 0.0004 | 0.0007 |
Bud | 0.0009 | 0.0023 | 0.0013 | 0.0009 | 0.0004 | 0.0004 | 0.0008 | 0.0001 | 0.0008 | 0.0005 | 0.0001 | 0.0002 | 0.0005 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.0001 |
Bud Neck | 0.0087 | 0.0006 | 0.0047 | 0.0026 | 0.0007 | 0.0046 | 0.008 | 0.0003 | 0.0008 | 0.0008 | 0.0014 | 0.0033 | 0.0027 | 0.0029 | 0.0009 | 0.0005 | 0.0012 | 0.004 |
Bud Periphery | 0.0017 | 0.0006 | 0.0019 | 0.0022 | 0.0011 | 0.0004 | 0.0012 | 0.0001 | 0.0005 | 0.0005 | 0 | 0.0003 | 0.0008 | 0.0001 | 0.0002 | 0 | 0.0001 | 0.0001 |
Bud Site | 0.0066 | 0.004 | 0.0065 | 0.0031 | 0.0006 | 0.0004 | 0.0109 | 0.0008 | 0.0015 | 0.0009 | 0.0001 | 0.0005 | 0.0018 | 0.0015 | 0.0009 | 0.0001 | 0.0005 | 0.0002 |
Cell Periphery | 0.0006 | 0.0002 | 0.0004 | 0.001 | 0.0001 | 0.0002 | 0.0004 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0001 | 0.0003 | 0.0001 | 0.0001 | 0 | 0 | 0 |
Cytoplasm | 0.1161 | 0.2083 | 0.1122 | 0.0525 | 0.1101 | 0.16 | 0.1058 | 0.1843 | 0.1574 | 0.135 | 0.1518 | 0.1936 | 0.1633 | 0.1638 | 0.1312 | 0.1905 | 0.1225 | 0.1812 |
Cytoplasmic Foci | 0.0382 | 0.0136 | 0.016 | 0.0086 | 0.0492 | 0.0121 | 0.0599 | 0.0105 | 0.0234 | 0.0122 | 0.0558 | 0.0195 | 0.0269 | 0.004 | 0.0042 | 0.0033 | 0.0058 | 0.0071 |
Eisosomes | 0.0009 | 0.0002 | 0.0002 | 0.0005 | 0.0002 | 0.0001 | 0.0002 | 0.0001 | 0.0004 | 0 | 0 | 0.0001 | 0.0004 | 0.0003 | 0.0002 | 0.0001 | 0 | 0 |
Endoplasmic Reticulum | 0.0121 | 0.0091 | 0.004 | 0.0034 | 0.0069 | 0.0025 | 0.0072 | 0.0041 | 0.0063 | 0.0036 | 0.0019 | 0.0037 | 0.0085 | 0.0031 | 0.0038 | 0.0034 | 0.0013 | 0.0024 |
Endosome | 0.0473 | 0.0112 | 0.014 | 0.0513 | 0.0837 | 0.0136 | 0.0575 | 0.0047 | 0.0206 | 0.0456 | 0.0799 | 0.018 | 0.0333 | 0.0025 | 0.0095 | 0.0033 | 0.0012 | 0.0056 |
Golgi | 0.0199 | 0.0004 | 0.0049 | 0.0332 | 0.0164 | 0.0119 | 0.0219 | 0.0002 | 0.0052 | 0.006 | 0.0175 | 0.0048 | 0.0098 | 0.0001 | 0.0011 | 0.0001 | 0.0001 | 0.0003 |
Lipid Particles | 0.0343 | 0.0026 | 0.0137 | 0.0185 | 0.0114 | 0.0073 | 0.0442 | 0.0008 | 0.0103 | 0.0079 | 0.0021 | 0.0055 | 0.0176 | 0.0005 | 0.0031 | 0.0002 | 0.0001 | 0.0017 |
Mitochondria | 0.0268 | 0.0011 | 0.0035 | 0.1027 | 0.0069 | 0.0093 | 0.0301 | 0.0009 | 0.0189 | 0.0041 | 0.0021 | 0.011 | 0.0033 | 0.0008 | 0.0018 | 0.0006 | 0.0004 | 0.0009 |
None | 0.3448 | 0.4716 | 0.4447 | 0.2678 | 0.2595 | 0.2299 | 0.2864 | 0.5384 | 0.2895 | 0.2624 | 0.4004 | 0.2089 | 0.2738 | 0.6152 | 0.2699 | 0.3285 | 0.4032 | 0.178 |
Nuclear Periphery | 0.0462 | 0.0186 | 0.0186 | 0.0106 | 0.0875 | 0.0141 | 0.022 | 0.0174 | 0.0226 | 0.0222 | 0.0082 | 0.0146 | 0.0273 | 0.0107 | 0.0181 | 0.029 | 0.0061 | 0.0158 |
Nucleolus | 0.005 | 0.0036 | 0.0104 | 0.0038 | 0.0044 | 0.0063 | 0.0039 | 0.0033 | 0.0037 | 0.0028 | 0.0025 | 0.0047 | 0.0029 | 0.0026 | 0.0113 | 0.0021 | 0.0018 | 0.0043 |
Nucleus | 0.18 | 0.197 | 0.2789 | 0.3729 | 0.2416 | 0.4521 | 0.2632 | 0.2067 | 0.3499 | 0.4335 | 0.23 | 0.4509 | 0.2881 | 0.1738 | 0.4982 | 0.4055 | 0.4017 | 0.5615 |
Peroxisomes | 0.0123 | 0.0005 | 0.0044 | 0.0043 | 0.0185 | 0.0014 | 0.0203 | 0.0005 | 0.0108 | 0.0178 | 0.0005 | 0.0026 | 0.0212 | 0.0001 | 0.0075 | 0.0001 | 0.0002 | 0.0003 |
Punctate Nuclear | 0.0391 | 0.048 | 0.051 | 0.0082 | 0.0826 | 0.0634 | 0.0215 | 0.0234 | 0.0581 | 0.0297 | 0.0405 | 0.0502 | 0.0411 | 0.0149 | 0.0304 | 0.0307 | 0.0524 | 0.032 |
Vacuole | 0.0075 | 0.0049 | 0.0039 | 0.0238 | 0.0055 | 0.0033 | 0.016 | 0.0026 | 0.0028 | 0.0103 | 0.0035 | 0.0032 | 0.0084 | 0.0021 | 0.0029 | 0.0011 | 0.0006 | 0.0028 |
Vacuole Periphery | 0.0032 | 0.0006 | 0.001 | 0.0101 | 0.0093 | 0.0006 | 0.0034 | 0.0005 | 0.0011 | 0.0023 | 0.0012 | 0.0009 | 0.0017 | 0.0005 | 0.0008 | 0.0004 | 0.0002 | 0.0008 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 6.0772 | 3.3068 | 4.9076 | 4.6764 | 4.111 | 4.7534 | 5.6123 | 4.8357 | 5.3008 | 6.49 |
Translational Efficiency | – | – | – | – | – | – | – | – | – | – |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | – |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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