Standard name
Human Ortholog
Description Protein involved in mismatch repair and meiotic recombination; only certain frameshift intermediates are mismatch repair substrates; forms a complex with Mlh1p

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.54 0.46 0.79 0.72 0.73 0.65 0.67 0.47 0.54 0.45 0.56 0.42 0.47 0.61 0.67 0.61 0.64 0.34 0.34 0.37 0.3 0.39 0.44
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0.05 0.07 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.28 0.19 0 0.07 0.05 0.26 0.27 0.55 0.45 0.56 0.34 0.25 0.07 0.06 0 0 0 0.08 0.05 0.07 0.06 0.09 0.09
Nucleus 0.09 0.15 0.11 0.14 0.11 0.1 0.11 0.12 0.09 0.08 0.09 0.27 0.2 0.14 0.08 0.09 0.05 0.21 0.25 0.14 0.14 0.09 0.05
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.27 0.31 0.11 0.09 0.12 0.09 0 0 0 0.05 0.07 0.19 0.35 0.25 0.3 0.34 0.34 0.28 0.3 0.33 0.43 0.32 0.3
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 2 0 2 0 0 0 0 0 0 0 0 0 0 0 0 2 0 2 0 1 4
Bud 1 0 0 0 1 4 3 14 10 17 3 2 0 1 0 0 0 1 0 3 4 6 12
Bud Neck 0 0 13 4 23 12 17 7 3 3 12 0 0 1 3 0 0 0 0 2 3 4 10
Bud Site 0 0 0 0 1 1 1 9 3 2 1 0 0 0 0 0 0
Cell Periphery 0 0 1 0 1 1 2 0 0 2 1 0 0 0 0 0 2 0 0 0 0 0 1
Cytoplasm 100 71 262 270 448 459 555 368 440 324 329 158 199 312 294 145 137 77 68 177 101 193 246
Endoplasmic Reticulum 1 2 2 2 0 0 2 0 0 0 0 1 0 1 32 11 16 0 0 0 2 6 6
Endosome 1 0 0 1 5 3 2 2 2 0 5 2 6 1 13 5 8 2 0 3 1 4 4
Golgi 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 1 0 1 1 0 2 0
Mitochondria 53 30 3 25 31 180 222 427 368 401 204 92 31 28 12 5 6 17 10 34 19 46 51
Nucleus 16 24 37 54 65 69 88 97 75 61 54 100 83 72 35 21 11 48 51 67 45 44 25
Nuclear Periphery 0 0 0 0 0 0 1 2 1 0 0 0 0 1 0 1 0 0 0 0 0 0 0
Nucleolus 1 0 0 0 0 0 1 1 1 1 3 1 0 0 0 0 1 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 1 3 10 6 5 5 11 3 21 0 0 1 0 3 0 9 3 12 6 13 18
Vac/Vac Membrane 50 49 36 33 71 60 36 32 35 36 40 73 148 125 133 81 73 65 61 157 144 160 170
Unique Cell Count 186 156 330 374 611 703 825 779 814 718 592 375 425 509 440 239 215 231 204 479 336 496 565
Labelled Cell Count 223 176 357 392 659 795 935 964 949 850 673 429 467 543 522 272 255 231 204 479 336 496 565


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.7 3.9 4.9 4.3 5.0 3.8 3.9 3.4 3.5 3.3 3.9 3.7 4.0 3.9 5.6 6.0 5.6 3.6 3.7 4.1
Std Deviation (1e-4) 0.5 0.7 0.8 1.0 1.2 0.9 1.1 0.7 0.8 0.7 0.7 0.8 0.8 0.7 1.1 1.2 1.0 0.7 1.2 1.3
Intensity Change (Log2) -0.17 0.02 -0.38 -0.31 -0.54 -0.49 -0.57 -0.35 -0.39 -0.31 -0.34 0.19 0.28 0.2 -0.45 -0.41 -0.25


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin
Bud 0 0 0
Bud Neck 0 0 0
Bud Site 0 0 0
Cell Periphery 0 0 0
Cyto
Endoplasmic Reticulum 0 0 0
Endosome 0 0 0
Golgi 0 0 0
Mitochondria 0 0 0
Nuclear Periphery 0 0 0
Nuc
Nucleolus 0 0 0
Peroxisomes 0 0 0
SpindlePole 0 0 0
Vac
Cortical Patches 0 0 0
Cytoplasm 0 0 0
Nucleus 0 0 0
Vacuole 6.4 6.7 6.6

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.1487 -0.5801 -0.6111 0.179 -0.6333 -0.4359 -0.4791 -0.9238 -0.6641 -0.6838 -1.1166 -0.7413 -0.3288 -0.63 -0.9922 -0.9841 -0.968 -0.802
Actin 0.0478 0.0011 0.0037 0.0181 0.0034 0.0061 0.0152 0.0003 0.015 0.0018 0.0004 0.0034 0.0662 0.0003 0.0039 0.0005 0.0004 0.0007
Bud 0.0009 0.0023 0.0013 0.0009 0.0004 0.0004 0.0008 0.0001 0.0008 0.0005 0.0001 0.0002 0.0005 0.0001 0.0002 0.0001 0.0001 0.0001
Bud Neck 0.0087 0.0006 0.0047 0.0026 0.0007 0.0046 0.008 0.0003 0.0008 0.0008 0.0014 0.0033 0.0027 0.0029 0.0009 0.0005 0.0012 0.004
Bud Periphery 0.0017 0.0006 0.0019 0.0022 0.0011 0.0004 0.0012 0.0001 0.0005 0.0005 0 0.0003 0.0008 0.0001 0.0002 0 0.0001 0.0001
Bud Site 0.0066 0.004 0.0065 0.0031 0.0006 0.0004 0.0109 0.0008 0.0015 0.0009 0.0001 0.0005 0.0018 0.0015 0.0009 0.0001 0.0005 0.0002
Cell Periphery 0.0006 0.0002 0.0004 0.001 0.0001 0.0002 0.0004 0.0001 0.0001 0.0001 0 0.0001 0.0003 0.0001 0.0001 0 0 0
Cytoplasm 0.1161 0.2083 0.1122 0.0525 0.1101 0.16 0.1058 0.1843 0.1574 0.135 0.1518 0.1936 0.1633 0.1638 0.1312 0.1905 0.1225 0.1812
Cytoplasmic Foci 0.0382 0.0136 0.016 0.0086 0.0492 0.0121 0.0599 0.0105 0.0234 0.0122 0.0558 0.0195 0.0269 0.004 0.0042 0.0033 0.0058 0.0071
Eisosomes 0.0009 0.0002 0.0002 0.0005 0.0002 0.0001 0.0002 0.0001 0.0004 0 0 0.0001 0.0004 0.0003 0.0002 0.0001 0 0
Endoplasmic Reticulum 0.0121 0.0091 0.004 0.0034 0.0069 0.0025 0.0072 0.0041 0.0063 0.0036 0.0019 0.0037 0.0085 0.0031 0.0038 0.0034 0.0013 0.0024
Endosome 0.0473 0.0112 0.014 0.0513 0.0837 0.0136 0.0575 0.0047 0.0206 0.0456 0.0799 0.018 0.0333 0.0025 0.0095 0.0033 0.0012 0.0056
Golgi 0.0199 0.0004 0.0049 0.0332 0.0164 0.0119 0.0219 0.0002 0.0052 0.006 0.0175 0.0048 0.0098 0.0001 0.0011 0.0001 0.0001 0.0003
Lipid Particles 0.0343 0.0026 0.0137 0.0185 0.0114 0.0073 0.0442 0.0008 0.0103 0.0079 0.0021 0.0055 0.0176 0.0005 0.0031 0.0002 0.0001 0.0017
Mitochondria 0.0268 0.0011 0.0035 0.1027 0.0069 0.0093 0.0301 0.0009 0.0189 0.0041 0.0021 0.011 0.0033 0.0008 0.0018 0.0006 0.0004 0.0009
None 0.3448 0.4716 0.4447 0.2678 0.2595 0.2299 0.2864 0.5384 0.2895 0.2624 0.4004 0.2089 0.2738 0.6152 0.2699 0.3285 0.4032 0.178
Nuclear Periphery 0.0462 0.0186 0.0186 0.0106 0.0875 0.0141 0.022 0.0174 0.0226 0.0222 0.0082 0.0146 0.0273 0.0107 0.0181 0.029 0.0061 0.0158
Nucleolus 0.005 0.0036 0.0104 0.0038 0.0044 0.0063 0.0039 0.0033 0.0037 0.0028 0.0025 0.0047 0.0029 0.0026 0.0113 0.0021 0.0018 0.0043
Nucleus 0.18 0.197 0.2789 0.3729 0.2416 0.4521 0.2632 0.2067 0.3499 0.4335 0.23 0.4509 0.2881 0.1738 0.4982 0.4055 0.4017 0.5615
Peroxisomes 0.0123 0.0005 0.0044 0.0043 0.0185 0.0014 0.0203 0.0005 0.0108 0.0178 0.0005 0.0026 0.0212 0.0001 0.0075 0.0001 0.0002 0.0003
Punctate Nuclear 0.0391 0.048 0.051 0.0082 0.0826 0.0634 0.0215 0.0234 0.0581 0.0297 0.0405 0.0502 0.0411 0.0149 0.0304 0.0307 0.0524 0.032
Vacuole 0.0075 0.0049 0.0039 0.0238 0.0055 0.0033 0.016 0.0026 0.0028 0.0103 0.0035 0.0032 0.0084 0.0021 0.0029 0.0011 0.0006 0.0028
Vacuole Periphery 0.0032 0.0006 0.001 0.0101 0.0093 0.0006 0.0034 0.0005 0.0011 0.0023 0.0012 0.0009 0.0017 0.0005 0.0008 0.0004 0.0002 0.0008

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 6.0772 3.3068 4.9076 4.6764 4.111 4.7534 5.6123 4.8357 5.3008 6.49
Translational Efficiency

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency
Standard name
Human Ortholog
Description Protein involved in mismatch repair and meiotic recombination; only certain frameshift intermediates are mismatch repair substrates; forms a complex with Mlh1p
Localization
Cell Percentages cytoplasm (14%), nucleus (8%), mixed (62%)
Cell Cycle Regulation Yes
cytoplasm - MA (3.5e-06)
Subcompartmental Group N/A

Mlh2

Mlh2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Mlh2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available