Standard name
Human Ortholog
Description Microtubule-associated protein (MAP) of the XMAP215/Dis1 family; regulates microtubule dynamics during spindle orientation and metaphase chromosome alignment; interacts with spindle pole body component Spc72p

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0.06 0 0.07 0.05 0 0 0 0 0 0 0 0 0 0 0.05 0.05
Bud Neck 0 0 0 0 0 0 0.05 0.05 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0.05 0 0.05 0 0 0.07 0.11 0.09 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0.06 0.05 0 0 0 0 0 0 0 0.06 0.08 0.07 0.1 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0.13 0 0 0 0 0 0 0 0 0 0.12 0.23 0.21 0.13 0.14 0.11 0.05 0.12 0.1 0.12 0.14 0.13
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.07 0 0 0 0 0.05 0.08 0 0.14 0.11 0.05 0 0 0 0.13 0 0.07 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.67 0.6 0.51 0.47 0.43 0.45 0.47 0.42 0.47 0.48 0.43 0.58 0.56 0.49 0.67 0.66 0.59 0.05 0.08 0 0 0 0
Peroxisomes 0.08 0.12 0 0 0 0.06 0 0 0 0 0 0.14 0.2 0.27 0.05 0 0.07 0 0 0 0.13 0.14 0.13
SpindlePole 0.57 0.62 0.89 0.83 0.77 0.71 0.73 0.72 0.63 0.7 0.75 0.59 0.57 0.5 0.38 0.48 0.48 0.74 0.63 0.74 0.55 0.49 0.5
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.07 0.06 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 1 2 1 1 1 0 3 0 0 1 1 0 2 2 0 0 1 1 0 3 10 12
Bud 1 3 3 3 2 11 17 22 9 17 17 3 0 0 3 1 1 3 5 1 9 35 34
Bud Neck 1 9 9 1 5 8 18 18 12 10 15 7 6 3 2 5 2 0 8 3 4 18 21
Bud Site 4 9 10 10 5 14 16 6 20 28 31 13 4 9 3 1 9
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 7 4 7 7 15 14 14 11 3 4 5 16 18 35 24 21 27 0 0 0 0 0 3
Endoplasmic Reticulum 0 0 0 0 1 0 1 0 0 0 0 0 0 0 0 3 5 0 0 0 0 4 0
Endosome 7 52 6 4 10 9 4 12 2 0 3 72 93 121 40 45 31 8 54 25 31 96 80
Golgi 0 0 1 0 0 0 0 0 0 0 0 0 0 0 1 2 2 1 3 0 1 6 4
Mitochondria 12 14 4 3 4 16 29 12 41 28 17 20 4 4 39 11 20 1 6 4 4 13 12
Nucleus 2 4 7 6 8 8 14 9 8 5 18 8 8 6 3 5 1 2 2 2 1 1 0
Nuclear Periphery 0 0 0 0 0 2 0 0 0 1 0 0 0 1 0 0 1 0 0 0 0 1 0
Nucleolus 113 244 127 102 103 136 179 160 142 123 140 337 228 275 206 210 168 7 37 9 5 14 12
Peroxisomes 13 48 4 9 4 18 3 13 9 9 10 79 80 154 15 12 20 4 18 7 32 93 80
SpindlePole 96 250 221 180 185 213 277 276 190 179 245 343 232 284 118 154 136 124 276 197 139 334 315
Vac/Vac Membrane 0 4 0 0 3 0 0 3 2 0 0 7 11 14 17 21 16 0 1 1 2 6 3
Unique Cell Count 169 406 248 217 239 302 380 382 301 257 328 581 406 563 307 320 284 168 442 267 255 677 632
Labelled Cell Count 257 642 401 326 346 450 572 545 438 404 502 906 684 908 473 491 439 168 442 267 255 677 632


SpindlePole

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 8.8 9.0 7.4 6.8 8.0 6.6 6.1 6.9 5.7 5.5 5.9 8.2 8.5 8.5 16.2 15.2 15.0 7.5 7.4 7.6
Std Deviation (1e-4) 1.7 1.8 1.1 1.4 2.0 1.6 1.4 1.3 1.1 1.6 1.4 1.7 2.3 2.3 5.6 4.3 4.5 1.5 1.6 1.8
Intensity Change (Log2) -0.12 0.11 -0.17 -0.27 -0.1 -0.38 -0.42 -0.32 0.16 0.21 0.21 1.13 1.04 1.02 0.03 -0.0 0.04


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 1.8 2.3 2.9 1.4 3.1 2.6 0 0 0 0 0 0
Bud Neck 0 -1.0 -0.7 0.7 0.7 0.2 0.2 0.6 0 -1.8 0 0 -1.6 -2.4
Bud Site 0.3 -1.2 0.3 0.1 -1.9 1.3 2.9 2.5 -1.4 -2.6 -2.1 -2.4 0 -0.5
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.3 1.8 1.1 0.6 0 0 -1.0 -1.1 -0.1 1.0 2.0 2.5 2.0 3.1
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 1.1 0.4 0 0.5 0 0 0 4.5 7.1 6.9 4.5 4.8 3.8
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 2.3 3.3 1.2 5.1 4.3 2.3 1.4 0 0 4.9 1.3 3.0
Nucleus 0 0.3 -0.1 0.6 -0.4 -0.1 -0.6 1.6 0 -0.7 0 0 -1.0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus -0.9 -1.8 -1.4 -1.0 -2.3 -0.9 -0.8 -2.0 1.8 1.2 -0.6 3.8 3.5 1.8
Peroxisomes 1.7 0 2.6 0 1.4 1.1 1.3 1.1 5.3 6.7 8.5 2.1 1.5 3.0
SpindlePole -1.9 -3.5 -5.3 -4.9 -5.1 -7.0 -5.4 -4.4 -8.5 -8.6 -10.5 -12.2 -10.2 -10.1
Vacuole 0 0 0 0 0 0 0 0 0 0 0 3.8 4.1 3.8

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 5.2098 6.5261 6.3919 6.5112 4.9229 6.1168 6.838 6.9533 7.0456 6.9056 6.6293 6.5318 6.0149 5.8792 6.0341 6.5386 6.2193 5.9871
Actin 0.0158 0.0009 0.0001 0.0001 0.0001 0.0002 0.0009 0.0003 0.0045 0.0048 0.0014 0.0006 0.0071 0.0001 0.0063 0.0027 0.0033 0.0001
Bud 0.0002 0.0005 0.0001 0 0 0 0.0002 0.0002 0.0002 0.0015 0.0003 0 0.0001 0.0001 0.0001 0.0001 0.0001 0
Bud Neck 0.0076 0.0013 0.0003 0.0021 0.0002 0.0002 0.0005 0.0009 0.0003 0.0009 0.0002 0.0002 0.005 0.0005 0.0006 0.0009 0.0019 0.0017
Bud Periphery 0.0004 0.0006 0.0002 0 0 0 0.0002 0.0001 0.0003 0.0011 0.0005 0.0001 0.0002 0.0001 0.0002 0.0002 0.0003 0
Bud Site 0.0035 0.0155 0.001 0.0001 0.0001 0.0001 0.0023 0.0073 0.0012 0.0013 0.0025 0.0001 0.0014 0.0009 0.0005 0.0012 0.0004 0.0001
Cell Periphery 0.0006 0.0002 0 0 0 0 0 0 0 0.0002 0.0001 0 0.0001 0 0 0.0001 0.0001 0
Cytoplasm 0.0023 0.0018 0.0005 0.0001 0.0001 0 0.0016 0.0001 0.0002 0.0032 0.0001 0.0001 0.0017 0.0001 0.0001 0.0019 0.0002 0.0001
Cytoplasmic Foci 0.0891 0.01 0.0274 0.0252 0.0874 0.0206 0.068 0.0359 0.0116 0.0387 0.0565 0.0129 0.0375 0.0049 0.0065 0.0135 0.0376 0.0104
Eisosomes 0.0002 0.0001 0 0 0 0 0 0 0 0 0.0001 0 0.0001 0 0.0001 0.0001 0.0001 0
Endoplasmic Reticulum 0.0008 0.0006 0.0009 0 0 0 0.0011 0 0.0001 0.0002 0.0006 0.0001 0.0009 0 0.0002 0.0029 0.0005 0
Endosome 0.0095 0.0053 0.0032 0.0004 0.0028 0.0003 0.0151 0.0014 0.0043 0.0063 0.0025 0.0016 0.0026 0.0001 0.0033 0.0197 0.0018 0.0019
Golgi 0.0047 0.0006 0.0006 0.0008 0.0048 0.0009 0.0081 0.0012 0.0038 0.0014 0.0033 0.0024 0.0032 0.0003 0.0021 0.0032 0.0026 0.0009
Lipid Particles 0.0492 0.0055 0.0137 0.0089 0.2385 0.0177 0.0425 0.013 0.014 0.0346 0.092 0.0147 0.0306 0.0023 0.0079 0.0184 0.0557 0.01
Mitochondria 0.0008 0.0005 0.0006 0 0.0002 0 0.0022 0.0001 0.0086 0.0133 0.0077 0.0031 0.0024 0.0003 0.0007 0.0021 0.0096 0.0037
None 0.0061 0.0014 0.0002 0.0001 0.0001 0 0.0008 0.0001 0.0003 0.0005 0.0003 0.0001 0.0038 0.0001 0.0002 0.0061 0.0004 0
Nuclear Periphery 0.0013 0.0106 0.0048 0.0008 0.0031 0.0005 0.0023 0.0009 0.0005 0.006 0.0025 0.0007 0.0022 0.001 0.0016 0.0096 0.0134 0.0008
Nucleolus 0.0112 0.118 0.0735 0.0276 0.015 0.014 0.0489 0.0659 0.0966 0.0213 0.0116 0.0251 0.0482 0.0872 0.0857 0.0226 0.0161 0.0389
Nucleus 0.0014 0.1626 0.0228 0.0028 0.0008 0.0019 0.0084 0.0245 0.014 0.0128 0.0017 0.0033 0.0102 0.0613 0.0242 0.0122 0.0129 0.0091
Peroxisomes 0.0149 0.0006 0.0012 0.0011 0.0053 0.0187 0.0057 0.0076 0.0033 0.0052 0.0028 0.0013 0.0072 0.0008 0.0016 0.0037 0.0139 0.0013
Punctate Nuclear 0.7802 0.6588 0.8465 0.9299 0.6407 0.9249 0.7903 0.8405 0.8357 0.8445 0.8122 0.9335 0.8352 0.8398 0.8577 0.8754 0.8279 0.9205
Vacuole 0.0003 0.0034 0.0017 0 0.0003 0 0.0004 0.0001 0.0004 0.0014 0.0006 0.0001 0.0003 0.0001 0.0002 0.0021 0.0004 0.0002
Vacuole Periphery 0.0002 0.0013 0.0008 0 0.0002 0 0.0003 0 0.0002 0.0008 0.0004 0.0002 0.0002 0.0001 0.0002 0.0011 0.0008 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 22.9617 35.8749 10.7032 13.5855 36.7443 27.2907 31.2248 22.3366 17.2587 28.4844
Translational Efficiency 1.6346 1.2993 1.3948 1.1032 0.8986 1.0965 1.0841 0.95 1.0207 1.0676

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency
Standard name
Human Ortholog
Description Microtubule-associated protein (MAP) of the XMAP215/Dis1 family; regulates microtubule dynamics during spindle orientation and metaphase chromosome alignment; interacts with spindle pole body component Spc72p
Localization
Cell Percentages spindle pole (26%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Stu2

Stu2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Stu2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available