Standard name
Human Ortholog
Description Ribosomal 60S subunit protein L22A; required for translation of long 5' UTR of IME1 mRNA and meiotic entry; required for the oxidative stress response, pseudohyphal and invasive growth; homologous to mammalian ribosomal protein L22, no bacterial homolog; RPL22A has a paralog, RPL22B, that arose from the whole genome duplication

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0.08 0 0 0 0 0 0 0
Cytoplasm 0.99 0.99 1.0 0.97 1.0 0.95 0.86 0.82 0.87 0.8 0.99 0.98 0.99 1.0 0.98 0.98 0.91 0.91 0.94 0.91 0.87 0.89
Endoplasmic Reticulum 0 0 0 0 0 0.05 0.14 0.18 0.11 0.14 0 0 0 0.07 0.09 0.08 0 0 0 0 0 0.05
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0
Bud 1 0 0 0 0 0 0 0 0 1 0 0 1 0 0 0 0 1 0 0 3 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 2 0 1 0 0 0 0 3 6 0 1 0 0 7 6 0 0 0 0 0 0
Cytoplasm 279 122 36 68 37 56 49 32 117 102 103 131 183 188 86 157 26 270 114 134 104 119
Endoplasmic Reticulum 1 0 0 1 1 3 8 7 15 18 0 2 1 14 8 13 0 2 3 3 2 7
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0
Golgi 2 0 0 0 0 0 0 0 1 2 0 0 0 0 0 0 0 0 0 0 2 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 1 1 0 0 0 0 0 0 1 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 1 0 0 0 0 0 0 0 0 1 0 1 2 0 1 0 1 8 0 2 0 1
Unique Cell Count 283 123 36 70 37 59 57 39 135 128 104 134 185 188 88 161 29 296 122 148 120 134
Labelled Cell Count 284 124 36 70 38 59 57 39 136 132 104 135 187 202 102 178 29 296 122 148 120 134


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 274.7 308.2 258.4 168.0 182.3 149.7 126.1 109.9 113.3 100.4 278.9 267.9 265.7 303.7 412.2 410.6 306.3 318.1 315.9
Std Deviation (1e-4) 71.0 63.6 78.3 44.9 38.5 40.7 34.1 28.8 30.5 26.7 68.1 77.4 55.8 120.1 141.4 128.1 71.3 117.1 107.0
Intensity Change (Log2) -0.25 -0.88 -0.76 -1.04 -1.29 -1.49 -1.44 -1.62 -0.14 -0.2 -0.21 -0.02 0.42 0.41 -0.01 0.05 0.04


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.5 -1.1 0.6 -1.8 -3.8 -4.3 -3.8 -5.0 -0.1 -0.9 -0.2 1.2 -0.9 -1.1
Endoplasmic Reticulum 0 0 0 0 0 0 3.8 4.3 0 0 0 3.1 0 3.2
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 247.4749 346.2064 287.7575 358.7399 266.1671 222.7764 266.2495 400.9431 343.2707 426.4071 374.3448 332.1406 277.2535 374.8028 330.7038 334.4824 292.2025 293.0423
Actin 0.0235 0.0036 0.0119 0.0063 0.0066 0.0149 0.0088 0.0023 0.0028 0.0015 0.0025 0.0128 0.0089 0.0018 0.0057 0.0027 0.0037 0.046
Bud 0.0068 0.0073 0.0092 0.0107 0.0196 0.0149 0.0067 0.0044 0.0066 0.0053 0.0055 0.003 0.0106 0.0061 0.0137 0.0221 0.015 0.0118
Bud Neck 0.0019 0.0016 0.0035 0.0098 0.0017 0.006 0.0025 0.0016 0.0026 0.0028 0.0027 0.0064 0.0019 0.0014 0.0036 0.0016 0.0037 0.0106
Bud Periphery 0.0102 0.0077 0.01 0.0085 0.0092 0.0134 0.0103 0.0042 0.0051 0.0026 0.0042 0.0049 0.0099 0.0033 0.0081 0.0089 0.0187 0.0147
Bud Site 0.0037 0.0079 0.0099 0.0021 0.0045 0.0033 0.0038 0.0055 0.0045 0.0011 0.0009 0.001 0.0056 0.0044 0.0056 0.0016 0.006 0.0026
Cell Periphery 0.1354 0.086 0.1391 0.0505 0.0411 0.0809 0.1229 0.147 0.1466 0.0461 0.0569 0.0828 0.0951 0.07 0.0692 0.146 0.1235 0.0368
Cytoplasm 0.2395 0.6099 0.2789 0.3466 0.2448 0.1546 0.2689 0.4591 0.3769 0.5888 0.262 0.2656 0.3076 0.7022 0.4317 0.5045 0.0788 0.2258
Cytoplasmic Foci 0.0116 0.0019 0.0053 0.0028 0.0048 0.0258 0.0112 0.0012 0.0059 0.001 0.0019 0.0109 0.0088 0.0013 0.0037 0.001 0.0018 0.0036
Eisosomes 0.0048 0.0005 0.0009 0.0001 0.0012 0.0036 0.0086 0.0004 0.0014 0 0.001 0.0026 0.0026 0.0031 0.0025 0.0011 0.0004 0.0019
Endoplasmic Reticulum 0.0373 0.0094 0.0412 0.0085 0.0055 0.016 0.0197 0.0087 0.0055 0.0019 0.0091 0.025 0.0097 0.004 0.0023 0.0011 0.0013 0.0059
Endosome 0.0068 0.0026 0.0144 0.0071 0.0045 0.0353 0.0053 0.002 0.0053 0.0017 0.0087 0.0052 0.0038 0.0011 0.0052 0.0013 0.0034 0.0069
Golgi 0.0021 0.0008 0.0032 0.0015 0.0007 0.0065 0.0018 0.0004 0.0006 0.0001 0.0031 0.0025 0.0011 0.0003 0.001 0.0003 0.0005 0.0052
Lipid Particles 0.0055 0.0023 0.0046 0.0021 0.0061 0.007 0.0094 0.0024 0.0049 0.0011 0.0107 0.0054 0.0047 0.0017 0.0046 0.0057 0.0059 0.0037
Mitochondria 0.0718 0.0254 0.051 0.0129 0.0858 0.1806 0.0565 0.0121 0.0495 0.0041 0.0798 0.1444 0.115 0.018 0.0579 0.0107 0.047 0.1158
None 0.0157 0.0024 0.0037 0.0013 0.005 0.0153 0.0188 0.0021 0.0083 0.0015 0.0015 0.008 0.0088 0.0032 0.0115 0.0021 0.0016 0.0058
Nuclear Periphery 0.0771 0.023 0.055 0.0177 0.0903 0.0653 0.0678 0.0178 0.047 0.0228 0.029 0.0946 0.0662 0.0139 0.0218 0.013 0.0078 0.0554
Nucleolus 0.0283 0.0111 0.0257 0.005 0.0556 0.0466 0.0343 0.0126 0.043 0.007 0.0117 0.0546 0.0331 0.0145 0.0696 0.0257 0.0934 0.0706
Nucleus 0.0549 0.0337 0.0427 0.0236 0.089 0.0639 0.0629 0.0422 0.0561 0.0485 0.0558 0.0527 0.0902 0.0328 0.0793 0.0473 0.0476 0.1054
Peroxisomes 0.0012 0.0008 0.0012 0.0008 0.0034 0.0024 0.0018 0.0005 0.0013 0.0004 0.0009 0.0015 0.0017 0.0009 0.0021 0.0006 0.002 0.0031
Punctate Nuclear 0.0008 0.0002 0.001 0.0002 0.0005 0.0017 0.0014 0.0001 0.0046 0.0002 0.0001 0.0011 0.0008 0.0001 0.0129 0.0001 0.0001 0.0007
Vacuole 0.1883 0.1251 0.2002 0.3217 0.1639 0.119 0.1934 0.225 0.1704 0.2013 0.2459 0.1104 0.1184 0.0926 0.1067 0.1504 0.3267 0.0892
Vacuole Periphery 0.0726 0.0369 0.0873 0.1599 0.1564 0.1232 0.0832 0.0484 0.0511 0.0603 0.2062 0.1046 0.0956 0.0235 0.0813 0.0522 0.2111 0.1781

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 2027.4303 2282.6793 2545.7693 2255.7754 2935.4909 2166.4169 1816.8463 1759.1405 1977.1567 1800.1048
Translational Efficiency 1.8886 1.7573 1.8822 2.032 1.8143 1.8291 1.9805 2.6575 2.2065 2.394

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency
Standard name
Human Ortholog
Description Ribosomal 60S subunit protein L22A; required for translation of long 5' UTR of IME1 mRNA and meiotic entry; required for the oxidative stress response, pseudohyphal and invasive growth; homologous to mammalian ribosomal protein L22, no bacterial homolog; RPL22A has a paralog, RPL22B, that arose from the whole genome duplication
Localization
Cell Percentages cytoplasm (97%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-1

Rpl22a

Rpl22a


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Rpl22a-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available