Standard name
Human Ortholog
Description Subunit of signal peptidase complex; complex catalyzes cleavage of N-terminal signal sequences of proteins targeted to the secretory pathway; homologous to mammalian SPC22/23; other members of the complex are Spc1p, Spc2p, and Sec11p

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0.07
Bud 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0.05 0.05 0.05
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0.06 0.06 0 0.09 0.13 0 0 0 0 0 0
Cytoplasm 0.22 0.33 0.36 0.33 0.33 0.32 0.36 0.32 0.28 0.31 0.34 0.33 0.37 0.3 0.05 0.1 0.06 0.12 0.07 0.09
Endoplasmic Reticulum 0.68 0.6 0.49 0.23 0.26 0.32 0.13 0.11 0.17 0.08 0.15 0.6 0.55 0.63 0.79 0.68 0.73 0.55 0.59 0.55
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0.21 0.52 0.43 0.46 0.51 0.53 0.48 0.59 0.57 0 0 0 0.05 0.07 0.07 0.1 0.11 0.11
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.12 0.09 0 0.06 0.17 0.09 0.17 0.2 0.21 0.17 0.09 0 0.05 0 0 0 0 0.09 0.07 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 5 5 7 9 5 9 5 7 6 4 7 3 2 6 4 7 8 1 3 6
Bud 2 1 2 12 8 10 11 15 14 21 17 0 0 1 2 6 1 3 4 4
Bud Neck 0 1 0 0 0 0 0 0 0 0 0 0 0 0 1 3 1 2 0 0
Bud Site 0 0 0 0 0 1 0 0 2 1 0 0 0 0
Cell Periphery 4 5 4 14 10 11 14 15 17 18 24 2 7 21 1 1 4 1 1 2
Cytoplasm 61 52 59 107 102 87 142 160 110 100 145 16 28 50 14 17 12 8 6 8
Endoplasmic Reticulum 185 96 80 75 80 87 53 56 70 25 64 29 41 106 247 120 143 42 58 53
Endosome 5 0 0 6 8 1 5 9 3 3 0 0 0 1 3 0 1 0 1 0
Golgi 2 1 0 1 0 0 0 0 0 0 0 0 0 2 4 0 0 0 0 3
Mitochondria 11 2 35 171 135 124 204 265 191 192 244 0 0 0 16 13 13 7 10 10
Nucleus 0 0 0 1 1 2 3 10 9 7 0 0 0 1 0 0 0 0 0 0
Nuclear Periphery 1 2 2 2 1 3 7 2 4 2 8 0 0 4 0 0 0 0 1 0
Nucleolus 1 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 1 0 0 0 0 1 0 0 1 0 0 1
Vac/Vac Membrane 34 14 5 21 53 25 66 98 84 56 39 2 4 1 13 4 5 6 7 4
Unique Cell Count 272 160 164 329 312 271 397 501 400 327 429 48 75 167 313 178 197 77 99 97
Labelled Cell Count 311 179 194 419 403 361 510 637 511 429 549 52 82 194 313 178 197 77 99 97


Endoplasmic Reticulum

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 7.5 7.1 5.3 5.5 5.7 5.6 5.3 5.4 5.1 5.1 5.3 8.3 7.9 8.1 8.4 8.7 8.8
Std Deviation (1e-4) 1.7 1.7 1.3 1.5 1.8 1.5 1.6 1.5 1.3 1.5 1.4 2.6 2.2 1.7 2.4 2.6 2.0
Intensity Change (Log2) 0.03 0.1 0.06 -0.02 0.0 -0.07 -0.07 -0.02 0.64 0.57 0.59 0.65 0.71 0.72


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches -0.9 0 -0.5 0 0 0 0 0 0 0 -0.3
Bud 0 0 0 0 0 0 2.6 1.7 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 1.0 0 0.9 0.7 0 1.0 1.5 1.6 0 0 3.5
Cytoplasm -0.8 -0.7 -0.8 0 -1.0 -2.0 -1.2 -0.5 -0.3 0.2 -1.2
Endoplasmic Reticulum -5.9 -5.1 -3.5 -9.0 -10.4 -7.6 -10.5 -8.6 1.4 0.8 2.7
Endosome 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 6.5 4.7 5.1 6.5 7.0 5.8 7.8 7.8 -3.5 -4.3 -6.3
Nucleus 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0
Vacuole 1.6 4.4 2.5 4.4 5.1 5.3 4.5 2.5 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 3.7882 3.8046 3.2772 2.8932 3.5686 3.6555 4.6373 5.4238 5.5489 4.4566 4.4104 4.9763 2.802 3.6572 2.9702 2.3958 3.0709 3.1861
Actin 0.0261 0.0011 0.002 0.0094 0.0003 0.002 0.0253 0.0034 0.0031 0.0065 0.0332 0.0007 0.0093 0.0007 0.0006 0.0022 0.0005 0.0015
Bud 0.0001 0.0001 0 0.0001 0 0.0001 0.0001 0.0014 0.0001 0.0001 0.0061 0.0001 0.0001 0.0001 0.0001 0.0001 0 0
Bud Neck 0.0006 0.0004 0.0012 0.0283 0.0011 0.0008 0.0004 0.0005 0.0002 0.0154 0.0008 0.0008 0.0003 0.0001 0.0003 0.0001 0.0001 0.0021
Bud Periphery 0.0002 0.0002 0.0001 0.0001 0.0001 0.0004 0.0002 0.0082 0.0001 0.0002 0.0035 0.0004 0.0002 0.0004 0.0002 0.0004 0 0.0001
Bud Site 0.0005 0.0017 0.0142 0.0003 0 0.0001 0.0003 0.0052 0.001 0.0003 0.0004 0.0004 0.0002 0.0005 0.0007 0.0001 0 0.0001
Cell Periphery 0.0009 0.0029 0.0018 0.0009 0.0008 0.0066 0.0026 0.0081 0.0026 0.0015 0.0018 0.0013 0.001 0.0017 0.0012 0.0021 0.0002 0.0008
Cytoplasm 0.092 0.17 0.1133 0.0251 0.0733 0.1042 0.0581 0.1249 0.1273 0.1711 0.0809 0.151 0.0627 0.102 0.0799 0.0713 0.1053 0.0982
Cytoplasmic Foci 0.0095 0.0042 0.0025 0.0184 0.0014 0.0122 0.0064 0.0073 0.0083 0.0146 0.009 0.0044 0.0062 0.0052 0.0046 0.0013 0.0023 0.0042
Eisosomes 0.0002 0.0002 0.0002 0.0001 0 0.0001 0.0014 0.0004 0.0002 0.0003 0.0043 0.0001 0.0005 0.0001 0.0001 0.0001 0 0.0003
Endoplasmic Reticulum 0.7552 0.7065 0.7788 0.8685 0.905 0.8016 0.8001 0.725 0.7731 0.6135 0.7369 0.7623 0.816 0.8141 0.8535 0.8435 0.8648 0.8072
Endosome 0.0208 0.0134 0.0078 0.0147 0.0068 0.0149 0.0177 0.0099 0.0057 0.0157 0.0323 0.0177 0.021 0.0165 0.0124 0.0033 0.0083 0.0106
Golgi 0.0049 0.0024 0.0009 0.0041 0.0008 0.0034 0.0094 0.0025 0.002 0.0037 0.0166 0.0029 0.0046 0.0013 0.0008 0.0008 0.0014 0.0013
Lipid Particles 0.0118 0.0104 0.0052 0.0045 0.0013 0.0053 0.0153 0.0129 0.0102 0.025 0.0089 0.0084 0.009 0.0056 0.0072 0.0023 0.0008 0.0041
Mitochondria 0.0012 0.0004 0.0003 0.0005 0.0002 0.0011 0.0017 0.0012 0.0008 0.0025 0.0016 0.0038 0.008 0.0009 0.0007 0.0004 0.0003 0.0056
None 0.0264 0.0625 0.0585 0.0007 0.0015 0.0339 0.0335 0.0709 0.0446 0.0586 0.0258 0.0321 0.0223 0.0308 0.0227 0.0607 0.0017 0.0465
Nuclear Periphery 0.0272 0.0139 0.0091 0.0174 0.0039 0.0086 0.0119 0.0085 0.0077 0.0173 0.0181 0.0063 0.021 0.009 0.0093 0.0067 0.0103 0.0092
Nucleolus 0.0003 0.0002 0 0 0 0.0001 0.0002 0.0002 0.0001 0.0002 0.0003 0.0002 0.0013 0.0001 0.0001 0 0 0.0001
Nucleus 0.0075 0.002 0.0007 0.001 0.0006 0.0009 0.0022 0.0024 0.001 0.0025 0.0046 0.0012 0.0065 0.0012 0.001 0.001 0.0015 0.0022
Peroxisomes 0.0031 0.0001 0.0001 0.0009 0 0.0003 0.0069 0.0003 0.0074 0.0421 0.0044 0.0002 0.001 0 0.0001 0 0 0.0001
Punctate Nuclear 0.007 0.001 0.0003 0.0011 0.0001 0.0011 0.0023 0.001 0.0006 0.0013 0.0037 0.0004 0.0033 0.0003 0.0004 0.0003 0.0002 0.0017
Vacuole 0.0034 0.006 0.0026 0.0029 0.0026 0.0021 0.003 0.0051 0.0035 0.0066 0.0056 0.0047 0.0041 0.0085 0.0038 0.0028 0.0019 0.0034
Vacuole Periphery 0.0009 0.0005 0.0004 0.0008 0.0003 0.0004 0.0008 0.0009 0.0004 0.001 0.0013 0.0007 0.0015 0.0008 0.0005 0.0003 0.0002 0.0005

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 76.5425 33.7678 31.6512 56.7102 48.9759 57.6234 53.9094 54.5778 44.9496 64.3702
Translational Efficiency 1.3503 1.8221 1.9996 1.2675 1.3431 1.4768 1.269 1.053 1.4057 1.1644

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency
Standard name
Human Ortholog
Description Subunit of signal peptidase complex; complex catalyzes cleavage of N-terminal signal sequences of proteins targeted to the secretory pathway; homologous to mammalian SPC22/23; other members of the complex are Spc1p, Spc2p, and Sec11p
Localization
Cell Percentages ER (97%)
Cell Cycle Regulation No
Subcompartmental Group ER-3

Spc3

Spc3


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Spc3-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available