Standard name
Human Ortholog
Description Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; required for glucose repression, HO repression, RME1 repression and sporulation
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0.05 0.07 0 0.05 0.09 0 0 0 0.05 0.1 0.11 0.12 0 0.11 0.08 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0.12 0.3 0.27 0.2 0.39 0.35 0.42 0.57 0.5 0.5 0.6 0 0 0.05 0 0.05 0 0 0 0 0 0 0
Nucleus 0.87 0.88 0.8 0.9 0.8 0.75 0.75 0.76 0.68 0.64 0.7 0.62 0.84 0.75 0.77 0.8 0.73 0.78 0.77 0.85 0.77 0.73 0.66 0.5
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.21 0.41 0.26 0.14 0.16 0.17 0.18 0.18 0.3 0.25 0.28 0.31 0.25 0.26 0.27 0.23 0.17 0.11 0.15 0.11 0.14 0.2 0.2 0.37
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0.05 0.07
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 1 0 1 1 1 1 2 2 3 0 1 0 0 0 0 0 0 0 0 0 3
Bud Neck 0 0 0 0 0 0 0 1 0 1 1 1 0 0 0 0 0 0 0 0 0 0 1 0
Bud Site 0 0 1 2 0 2 9 8 6 13 5 8 0 1 1 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 3 1 5 8 10 5 14 23 5 12 5 12 15 29 31 8 15 12 0 0 0 0 1 0
Endoplasmic Reticulum 0 0 1 0 0 0 0 0 0 0 1 0 0 0 0 1 0 2 0 0 1 0 1 0
Endosome 1 0 0 0 0 0 0 0 0 0 0 0 0 1 1 1 1 0 1 0 1 1 3 1
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 1 4 0 0 0 0 2 0
Mitochondria 2 13 38 40 29 57 89 103 90 143 90 146 5 3 12 6 7 6 0 0 0 0 1 1
Nucleus 109 95 101 132 118 109 193 185 108 185 126 151 131 194 201 146 96 113 105 95 100 64 144 107
Nuclear Periphery 0 1 1 0 2 2 5 2 4 1 7 5 1 3 3 2 0 1 0 0 0 0 1 0
Nucleolus 26 44 33 20 23 25 45 44 48 73 51 75 39 68 69 42 23 16 20 12 17 17 43 79
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 1 2 0 0 0 0 0 0 0 0 0
SpindlePole 1 0 0 1 2 1 2 0 0 2 0 0 0 2 1 1 0 0 2 0 0 0 3 2
Vac/Vac Membrane 0 0 0 1 1 2 2 0 1 2 0 3 0 2 2 5 6 2 2 2 4 1 10 14
Unique Cell Count 126 108 126 146 148 146 256 244 158 287 180 245 156 257 260 182 132 144 137 113 131 89 217 214
Labelled Cell Count 142 154 180 205 185 204 360 367 263 434 288 404 191 305 323 214 149 156 137 113 131 89 217 214


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 8.6 6.1 5.1 5.3 5.1 4.9 4.6 4.3 4.2 4.2 4.1 3.9 7.5 7.2 6.4 9.0 10.1 10.7 7.1 8.0 8.7
Std Deviation (1e-4) 1.5 1.2 1.0 1.7 1.4 1.8 1.4 1.1 1.4 1.1 1.1 1.4 2.4 2.2 1.8 1.9 2.8 2.5 1.4 1.6 1.6
Intensity Change (Log2) – – – 0.06 0.01 -0.05 -0.13 -0.26 -0.28 -0.28 -0.3 -0.39 0.56 0.51 0.33 0.82 0.99 1.07 0.48 0.64 0.77


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.6 1.0 0 0.6 1.9 0 0.1 0 0.4 1.8 2.4 2.5 0.2 2.2 1.5
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria -0.5 -2.0 1.5 0 0 0 0 0 0 -6.3 -8.6 -7.0 -6.6 -5.3 -5.8
Nucleus 2.4 -0.1 -1.1 -1.0 -0.9 -2.2 -3.2 -2.0 -3.6 0.8 -1.0 -0.6 0 -1.4 -0.3
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus -2.6 -2.2 -1.8 0 0 0 0 0 0 -0.2 0.1 0.1 -0.6 -1.7 -3.2
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 3.6937 4.5142 3.7592 3.2952 2.8341 3.7364 3.8605 4.9201 4.5849 5.1585 3.4335 4.2867 5.6084 5.1148 5.7058 5.1192 5.1475 5.4586
Actin 0.0834 0 0.0185 0.0014 0.0184 0.0061 0.0591 0 0.0126 0.0005 0.0053 0.0045 0.0001 0 0.0013 0.0001 0.0012 0
Bud 0.0009 0 0.0002 0.0004 0.0002 0.0001 0.0009 0.0001 0.0003 0.0001 0.0005 0.0002 0 0 0.0001 0.0003 0.0002 0
Bud Neck 0.0054 0.0001 0.0018 0.0122 0.0019 0.0016 0.0016 0.0002 0.0003 0.0001 0.0033 0.0014 0.0003 0.0001 0.0036 0.0016 0.0018 0.0009
Bud Periphery 0.0017 0 0.0004 0.0018 0.0005 0.0002 0.0021 0.0001 0.0003 0.0001 0.0009 0.0006 0.0001 0.0001 0.0002 0.0007 0.0006 0
Bud Site 0.0039 0.0001 0.0035 0.0011 0.0009 0.0002 0.0072 0.001 0.0011 0.0001 0.0012 0.0006 0.0001 0.0002 0.0206 0.0004 0.0006 0.0001
Cell Periphery 0.0006 0 0.0002 0.0016 0.0001 0.0001 0.001 0 0 0 0.0001 0.0002 0 0 0.0004 0.0001 0.0001 0
Cytoplasm 0.0081 0 0.0006 0.0001 0.002 0.0005 0.0056 0.0001 0.003 0.0003 0.0002 0.0011 0.0001 0 0.0006 0.0001 0.0002 0
Cytoplasmic Foci 0.016 0 0.0083 0.0002 0.0474 0.0006 0.0205 0.0101 0.012 0.0088 0.003 0.0017 0.0001 0 0.0291 0 0.0002 0
Eisosomes 0.0008 0 0.0002 0.0002 0.0003 0.0001 0.0008 0 0.0001 0 0.0001 0 0 0 0 0 0.0001 0
Endoplasmic Reticulum 0.0046 0 0.0007 0.0002 0.0023 0.0001 0.0049 0 0.0008 0.0003 0.0001 0.0018 0 0 0.0003 0 0.0002 0
Endosome 0.0226 0 0.006 0.0019 0.0354 0.0017 0.0209 0.0001 0.0085 0.0038 0.0078 0.0159 0.0001 0 0.0005 0.0001 0.0007 0
Golgi 0.0112 0 0.0027 0.0008 0.0169 0.0033 0.0135 0.0001 0.0029 0.0021 0.0108 0.0021 0 0 0.0004 0 0.0003 0
Lipid Particles 0.0207 0 0.0092 0.0009 0.0683 0.002 0.0206 0.0009 0.0048 0.063 0.021 0.003 0 0 0.0002 0 0.0012 0
Mitochondria 0.0433 0.0002 0.0035 0.0417 0.008 0.003 0.0162 0.0005 0.002 0.0011 0.067 0.0039 0.0003 0.0002 0.0003 0.0003 0.0035 0.0001
None 0.0048 0 0.0007 0.0001 0.0037 0.0004 0.0103 0 0.0037 0.0003 0.0001 0.0003 0.0001 0 0.0001 0 0.0005 0
Nuclear Periphery 0.0215 0.0004 0.0055 0.0083 0.0174 0.0007 0.0144 0.0028 0.0017 0.0027 0.0006 0.0076 0.0007 0.0009 0.0004 0.0007 0.071 0.0001
Nucleolus 0.0835 0.054 0.0453 0.03 0.2082 0.1397 0.05 0.0603 0.0457 0.0244 0.1823 0.0758 0.0492 0.0834 0.0559 0.0835 0.3615 0.1104
Nucleus 0.6387 0.9436 0.8739 0.8663 0.472 0.8318 0.7125 0.9203 0.8788 0.8853 0.6858 0.8631 0.9429 0.9133 0.8839 0.91 0.5305 0.887
Peroxisomes 0.0061 0 0.0098 0.0004 0.0445 0.0005 0.0229 0.0014 0.0111 0.0011 0.0059 0.0006 0 0 0.0003 0 0.0003 0
Punctate Nuclear 0.0127 0.0013 0.0071 0.0178 0.049 0.0069 0.0085 0.0016 0.0096 0.0048 0.0021 0.0125 0.0056 0.0014 0.0013 0.0013 0.023 0.0013
Vacuole 0.0062 0.0001 0.0011 0.0099 0.001 0.0002 0.0049 0.0001 0.0004 0.0004 0.001 0.0018 0.0001 0.0002 0.0003 0.0005 0.0006 0.0001
Vacuole Periphery 0.0034 0.0001 0.0007 0.0028 0.0016 0.0001 0.0017 0 0.0002 0.0006 0.0009 0.0012 0 0.0001 0.0001 0.0002 0.0019 0

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 21.7353 22.4659 22.528 29.6112 29.7219 24.7781 23.6362 25.7939 26.4417 32.2833
Translational Efficiency 0.6744 0.5586 0.6345 0.4494 0.4397 0.5448 0.5886 0.4034 0.5177 0.4761

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; required for glucose repression, HO repression, RME1 repression and sporulation
Localization
Cell Percentages nucleus (94%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-4

Rgr1

Rgr1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Rgr1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available