Standard name | |
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Human Ortholog | |
Description | Cyclin-dependent kinase inhibitor (CKI); inhibitor of Cdc28-Clb kinase complexes that controls G1/S phase transition, preventing premature S phase and ensuring genomic integrity; phosphorylated by Clb5/6-Cdk1 and Cln1/2-Cdk1 kinase which regulate timing of Sic1p degradation; phosphorylation targets Sic1p for SCF(CDC4)-dependent turnover; functional homolog of mammalian Kip1 |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 1 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | 0 | 1 | 2 | 0 | 0 | 1 |
Bud | 0 | 0 | 2 | 0 | 2 | 2 | 7 | – | 12 | 4 | 5 | 5 | 0 | 0 | 0 | – | – | – | 16 | 14 | 29 | 5 | 13 | 10 |
Bud Neck | 0 | 0 | 4 | 1 | 1 | 2 | 0 | – | 2 | 2 | 2 | 0 | 0 | 0 | 0 | – | – | – | 0 | 1 | 3 | 1 | 3 | 1 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 1 | 0 | 4 | 0 | 0 | 0 | – | – | – | – | – | – | – | – | – |
Cell Periphery | 0 | 2 | 1 | 0 | 0 | 1 | 0 | – | 0 | 0 | 1 | 1 | 0 | 0 | 0 | – | – | – | 0 | 1 | 1 | 0 | 0 | 0 |
Cytoplasm | 84 | 196 | 225 | 135 | 167 | 178 | 164 | – | 51 | 83 | 66 | 104 | 75 | 113 | 103 | – | – | – | 55 | 145 | 157 | 30 | 42 | 48 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 1 | 0 | 2 | – | 0 | 0 | 0 | 0 | 0 | 2 | 0 | – | – | – | 0 | 0 | 2 | 2 | 2 | 1 |
Endosome | 3 | 0 | 8 | 1 | 2 | 7 | 4 | – | 0 | 1 | 0 | 2 | 0 | 1 | 3 | – | – | – | 2 | 8 | 3 | 1 | 2 | 6 |
Golgi | 2 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | 3 | 3 | 3 | 2 | 0 | 1 |
Mitochondria | 5 | 6 | 0 | 3 | 5 | 6 | 13 | – | 52 | 38 | 40 | 48 | 3 | 1 | 1 | – | – | – | 2 | 10 | 5 | 12 | 20 | 14 |
Nucleus | 27 | 36 | 58 | 36 | 29 | 75 | 111 | – | 62 | 55 | 93 | 127 | 11 | 22 | 8 | – | – | – | 39 | 50 | 63 | 36 | 59 | 40 |
Nuclear Periphery | 0 | 0 | 0 | 1 | 0 | 1 | 0 | – | 0 | 0 | 0 | 1 | 0 | 1 | 0 | – | – | – | 0 | 0 | 0 | 0 | 1 | 1 |
Nucleolus | 1 | 1 | 0 | 1 | 0 | 0 | 2 | – | 3 | 3 | 9 | 9 | 0 | 0 | 0 | – | – | – | 1 | 2 | 1 | 0 | 2 | 2 |
Peroxisomes | 0 | 0 | 1 | 0 | 0 | 1 | 0 | – | 0 | 1 | 1 | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 | 0 | 0 | 1 |
SpindlePole | 0 | 1 | 1 | 0 | 2 | 4 | 4 | – | 0 | 2 | 3 | 3 | 0 | 0 | 0 | – | – | – | 10 | 17 | 24 | 6 | 2 | 6 |
Vac/Vac Membrane | 20 | 33 | 27 | 7 | 20 | 31 | 22 | – | 5 | 23 | 27 | 21 | 8 | 12 | 17 | – | – | – | 7 | 16 | 14 | 8 | 20 | 18 |
Unique Cell Count | 131 | 256 | 302 | 178 | 216 | 290 | 312 | 139 | 187 | 216 | 276 | 90 | 145 | 119 | 144 | 279 | 317 | 112 | 174 | 162 | ||||
Labelled Cell Count | 142 | 275 | 327 | 185 | 229 | 308 | 330 | 187 | 213 | 247 | 325 | 97 | 152 | 132 | 144 | 279 | 317 | 112 | 174 | 162 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 5.4 | 4.5 | 6.1 | 6.1 | 6.3 | 5.6 | 5.8 | – | 4.9 | 5.0 | 5.8 | 5.5 | 5.5 | 6.5 | 5.8 | – | – | – | 7.3 | 7.7 | 7.4 |
Std Deviation (1e-4) | 2.1 | 1.6 | 1.7 | 2.7 | 2.4 | 2.4 | 2.3 | – | 1.8 | 1.8 | 2.8 | 1.9 | 1.6 | 2.6 | 2.2 | – | – | – | 2.5 | 2.4 | 2.4 |
Intensity Change (Log2) | – | – | – | 0.0 | 0.04 | -0.13 | -0.08 | – | -0.33 | -0.27 | -0.06 | -0.15 | -0.15 | 0.08 | -0.06 | – | – | – | 0.26 | 0.33 | 0.27 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 5.707 | 4.9818 | 4.1039 | 3.5103 | 4.1028 | 4.8315 | 4.7635 | 5.287 | 3.3161 | 2.9439 | 4.7475 | 4.3299 | 3.5057 | 1.5431 | 1.0216 | 1.0145 | 0.7 | 1.9656 |
Actin | 0.053 | 0.014 | 0.0083 | 0.0214 | 0.1249 | 0.0026 | 0.0121 | 0.0001 | 0.0184 | 0.0003 | 0.0005 | 0.0003 | 0.1495 | 0.0011 | 0.032 | 0.0461 | 0.0204 | 0.0298 |
Bud | 0.0013 | 0.0123 | 0.0173 | 0.004 | 0.0023 | 0.0022 | 0.0005 | 0 | 0.0006 | 0.0012 | 0.0005 | 0.0008 | 0.001 | 0.0056 | 0.0176 | 0.0017 | 0.0006 | 0.0011 |
Bud Neck | 0.004 | 0.0016 | 0.0017 | 0.0409 | 0.0013 | 0.0069 | 0.0003 | 0 | 0.0013 | 0.0011 | 0.0009 | 0.0019 | 0.0033 | 0.0004 | 0.0012 | 0.0012 | 0.001 | 0.0021 |
Bud Periphery | 0.0016 | 0.008 | 0.0151 | 0.0059 | 0.0028 | 0.0023 | 0.0007 | 0 | 0.0009 | 0.0012 | 0.0003 | 0.0011 | 0.0015 | 0.0034 | 0.014 | 0.0017 | 0.0004 | 0.0012 |
Bud Site | 0.0166 | 0.0305 | 0.0071 | 0.023 | 0.002 | 0.0005 | 0.0023 | 0 | 0.0036 | 0.0009 | 0.0014 | 0.0003 | 0.0119 | 0.0039 | 0.0054 | 0.0008 | 0.0024 | 0.0018 |
Cell Periphery | 0.0005 | 0.001 | 0.001 | 0.0005 | 0.0003 | 0.0001 | 0.0004 | 0.0001 | 0.0003 | 0.0009 | 0.0002 | 0.0001 | 0.0005 | 0.0006 | 0.0006 | 0.0002 | 0.0001 | 0.0001 |
Cytoplasm | 0.2418 | 0.2537 | 0.1707 | 0.2429 | 0.2229 | 0.3704 | 0.1907 | 0.106 | 0.1762 | 0.2119 | 0.3162 | 0.2317 | 0.0947 | 0.4007 | 0.2311 | 0.132 | 0.1232 | 0.3282 |
Cytoplasmic Foci | 0.0339 | 0.0038 | 0.0236 | 0.0222 | 0.0039 | 0.0036 | 0.0265 | 0.0023 | 0.0199 | 0.0088 | 0.0017 | 0.0175 | 0.0173 | 0.0072 | 0.0047 | 0.0094 | 0.017 | 0.0061 |
Eisosomes | 0.0006 | 0.0001 | 0.0004 | 0.0002 | 0.0008 | 0 | 0.0003 | 0.0003 | 0.0005 | 0.0003 | 0 | 0 | 0.0026 | 0.0001 | 0.0002 | 0.0002 | 0.0002 | 0.0001 |
Endoplasmic Reticulum | 0.0098 | 0.0074 | 0.0081 | 0.002 | 0.0165 | 0.0035 | 0.0205 | 0.0022 | 0.0027 | 0.0056 | 0.0071 | 0.0037 | 0.0076 | 0.0059 | 0.0056 | 0.0049 | 0.0031 | 0.003 |
Endosome | 0.0663 | 0.0161 | 0.0194 | 0.0189 | 0.015 | 0.0024 | 0.1498 | 0.0008 | 0.0201 | 0.0025 | 0.0024 | 0.0105 | 0.0168 | 0.0019 | 0.0039 | 0.0082 | 0.0345 | 0.0073 |
Golgi | 0.0279 | 0.0006 | 0.0123 | 0.0425 | 0.0455 | 0.0001 | 0.012 | 0.0002 | 0.0031 | 0.0001 | 0.0001 | 0.0003 | 0.0128 | 0.0002 | 0.0015 | 0.0037 | 0.0095 | 0.0057 |
Lipid Particles | 0.0139 | 0.0005 | 0.0228 | 0.014 | 0.0066 | 0.0001 | 0.0263 | 0.0002 | 0.0194 | 0.0016 | 0.0002 | 0.0006 | 0.01 | 0.001 | 0.0009 | 0.0035 | 0.0032 | 0.003 |
Mitochondria | 0.0035 | 0.0018 | 0.0051 | 0.0183 | 0.0215 | 0.0008 | 0.0044 | 0.0001 | 0.0022 | 0.0002 | 0.0004 | 0.0004 | 0.0034 | 0.002 | 0.0036 | 0.0012 | 0.0025 | 0.0012 |
None | 0.2543 | 0.3746 | 0.5219 | 0.5204 | 0.4441 | 0.2756 | 0.5003 | 0.7208 | 0.7067 | 0.7189 | 0.5511 | 0.3792 | 0.1274 | 0.4341 | 0.5662 | 0.75 | 0.7279 | 0.3352 |
Nuclear Periphery | 0.016 | 0.0084 | 0.028 | 0.0014 | 0.0198 | 0.0129 | 0.0248 | 0.0002 | 0.0022 | 0.0022 | 0.0044 | 0.0063 | 0.029 | 0.005 | 0.0088 | 0.0055 | 0.0059 | 0.0078 |
Nucleolus | 0.0021 | 0.0025 | 0.006 | 0.0004 | 0.0007 | 0.0028 | 0.0011 | 0.0003 | 0.002 | 0.0048 | 0.0009 | 0.0015 | 0.0027 | 0.0018 | 0.0011 | 0.0004 | 0.0053 | 0.0018 |
Nucleus | 0.2069 | 0.2456 | 0.0913 | 0.0042 | 0.0583 | 0.3007 | 0.0044 | 0.166 | 0.0019 | 0.017 | 0.1058 | 0.3303 | 0.4633 | 0.1176 | 0.0743 | 0.0203 | 0.0107 | 0.2518 |
Peroxisomes | 0.009 | 0.0002 | 0.0093 | 0.0113 | 0.0012 | 0.0001 | 0.0039 | 0 | 0.0067 | 0.0001 | 0 | 0.0003 | 0.012 | 0.0002 | 0.0005 | 0.0036 | 0.0035 | 0.0025 |
Punctate Nuclear | 0.0319 | 0.0058 | 0.0201 | 0.0023 | 0.003 | 0.0095 | 0.0081 | 0.0001 | 0.0078 | 0.0105 | 0.0015 | 0.011 | 0.0299 | 0.0039 | 0.0218 | 0.003 | 0.0267 | 0.0087 |
Vacuole | 0.0035 | 0.0096 | 0.0081 | 0.0022 | 0.0044 | 0.0023 | 0.0067 | 0.0002 | 0.0031 | 0.0098 | 0.0039 | 0.0018 | 0.0014 | 0.0028 | 0.0039 | 0.0018 | 0.0012 | 0.0012 |
Vacuole Periphery | 0.0015 | 0.0018 | 0.0024 | 0.0007 | 0.0023 | 0.0005 | 0.0038 | 0 | 0.0004 | 0.0004 | 0.0005 | 0.0003 | 0.0014 | 0.0005 | 0.001 | 0.0005 | 0.0005 | 0.0003 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 12.26 | 9.2293 | 46.2274 | 99.0732 | 45.1772 | 23.2167 | 16.3305 | 22.4375 | 75.2048 | 57.4961 |
Translational Efficiency | 2.0215 | 1.5385 | 1.4527 | 1.3971 | 1.2847 | 1.2276 | 1.1266 | 1.3571 | 1.6375 | 1.419 |
Dataset | Hit |
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Protein Concentration | ✔ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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