Standard name
Human Ortholog
Description Integral membrane component of ER-derived COPII-coated vesicles; functions in ER to Golgi transport; EMP46 has a paralog, EMP47, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.47 0.53 0.74 0.89 0.81 0.87 0.87 0.89 0.85 0.82 0.81 0.81 0.82 0.73 0.69 0.2 0.25 0.13 0.06 0.09 0.07 0.17 0.21 0.14
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.1 0 0.06 0 0 0 0.08 0.06 0.05 0.06
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.26 0.05 0 0.05 0.07 0.13 0.17 0.12 0.15 0.19 0.27 0.21 0 0 0 0 0 0 0 0 0.07 0 0 0
Nucleus 0 0.07 0 0 0 0 0 0 0 0 0 0 0 0 0 0.19 0.18 0.23 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.47 0.52 0.33 0.08 0.2 0.12 0.09 0.1 0.1 0.15 0.14 0.15 0.16 0.34 0.33 0.74 0.68 0.75 0.83 0.83 0.72 0.69 0.66 0.71
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 1 0 0 2 0 0 0 0 0 0 0 1 0 0 0 0 0 1 0 0 1
Bud 0 1 1 4 2 6 19 20 13 15 8 13 1 0 1 0 0 0 0 1 0 1 0 7
Bud Neck 0 0 1 0 0 0 0 0 0 2 0 0 1 0 0 0 0 0 0 0 1 0 1 0
Bud Site 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 1 0 0 0 1 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 93 120 187 272 264 370 559 627 409 508 356 385 188 276 295 28 26 9 13 23 21 36 82 64
Endoplasmic Reticulum 3 2 2 3 0 0 2 0 0 1 0 0 2 1 5 2 3 2 1 0 2 0 1 2
Endosome 2 3 9 10 11 9 4 10 4 1 2 3 6 24 42 1 6 0 9 8 22 12 20 29
Golgi 0 0 1 1 0 0 0 0 0 0 0 0 0 0 0 1 0 1 0 0 4 0 4 2
Mitochondria 52 12 7 14 24 55 111 88 71 119 120 98 0 0 1 3 3 0 5 4 20 3 8 9
Nucleus 4 15 1 2 0 1 4 1 1 5 5 0 3 5 2 27 19 16 1 3 0 3 3 3
Nuclear Periphery 2 0 0 2 0 1 6 4 0 2 1 3 0 0 0 0 0 0 0 0 3 0 0 1
Nucleolus 1 0 0 1 0 1 0 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
SpindlePole 0 0 0 1 0 1 2 1 1 0 0 2 1 0 0 0 1 0 0 0 0 4 5 5
Vac/Vac Membrane 94 117 85 25 66 51 56 69 50 92 63 71 37 129 142 103 71 53 186 207 205 148 253 332
Unique Cell Count 198 226 254 304 326 423 646 708 481 618 438 477 228 378 426 140 104 71 223 251 287 216 386 471
Labelled Cell Count 251 270 294 336 368 495 766 821 551 745 555 576 239 435 489 165 129 81 223 251 287 216 386 471


Vacuole

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.8 4.7 4.6 4.3 4.7 4.3 4.0 4.0 4.0 3.9 3.7 3.7 5.6 5.7 5.7 7.6 6.9 7.5 5.7 5.6 6.1
Std Deviation (1e-4) 0.6 0.9 1.0 1.6 1.8 1.7 1.2 0.9 1.4 1.0 1.3 0.9 1.0 1.2 1.1 1.9 1.6 1.7 1.4 1.4 1.6
Intensity Change (Log2) – – – -0.08 0.04 -0.09 -0.19 -0.2 -0.17 -0.23 -0.3 -0.3 0.29 0.31 0.31 0.74 0.61 0.72 0.32 0.29 0.42


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – 0 0 0
Bud Neck – – – – – – – – – – – – 0 0 0
Bud Site – – – – – – – – – – – – 0 0 0
Cell Periphery – – – – – – – – – – – – 0 0 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – 0 0 0
Endosome – – – – – – – – – – – – 0 0 0
Golgi – – – – – – – – – – – – 0 0 0
Mitochondria – – – – – – – – – – – – 0 0 0
Nuclear Periphery – – – – – – – – – – – – 0 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – 0 0 0
Peroxisomes – – – – – – – – – – – – 0 0 0
SpindlePole – – – – – – – – – – – – 0 0 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – 0 0 0
Cytoplasm – – – – – – – – – – – – 0 0 0
Nucleus – – – – – – – – – – – – 0 0 0
Vacuole – – – – – – – – – – – – 7.6 6.0 6.2

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.103 2.1964 1.5989 1.483 1.3841 1.7898 0.1715 0.4371 0.3127 0.4583 -0.0519 -0.2179 -1.543 -0.7978 -1.2866 -0.7575 -1.3233 -1.1385
Actin 0.0114 0 0.0067 0.0001 0.0049 0.0001 0.0386 0.0034 0.0235 0.018 0.0062 0.0074 0.0498 0.0001 0.0157 0.0268 0.0002 0.0009
Bud 0.0004 0.0003 0.0005 0.0004 0.0002 0.0003 0.0009 0.0032 0.0013 0.0021 0.002 0.0003 0.0007 0.004 0.0008 0.0009 0.0002 0.0003
Bud Neck 0.0025 0.0003 0.0006 0.0003 0.0004 0.0005 0.0026 0.0014 0.001 0.0005 0.0023 0.001 0.0013 0.0007 0.0007 0.0004 0.0002 0.0006
Bud Periphery 0.0006 0.0004 0.0009 0.0006 0.0004 0.0006 0.0015 0.003 0.0013 0.0162 0.0036 0.0003 0.0007 0.0025 0.0008 0.0016 0.0002 0.0003
Bud Site 0.0029 0.0009 0.001 0.0005 0.0006 0.0003 0.0059 0.0132 0.0063 0.004 0.0058 0.0005 0.0021 0.0073 0.0018 0.0007 0.0006 0.0005
Cell Periphery 0.0007 0.0005 0.0006 0.0003 0.0003 0.0002 0.0007 0.0006 0.0004 0.0012 0.0004 0.0001 0.0003 0.0004 0.0003 0.0002 0.0001 0.0001
Cytoplasm 0.1237 0.1783 0.1769 0.0749 0.1222 0.2201 0.1743 0.2638 0.2445 0.1828 0.2109 0.281 0.2166 0.2392 0.178 0.1683 0.1691 0.3198
Cytoplasmic Foci 0.0633 0.0458 0.0493 0.0383 0.0685 0.0473 0.0473 0.0497 0.0667 0.0261 0.0531 0.0621 0.0702 0.0598 0.084 0.0654 0.0905 0.066
Eisosomes 0.0002 0.0001 0.0002 0.0001 0.0001 0 0.0002 0 0.0001 0.0006 0.0001 0.0001 0.0004 0 0.0005 0.0002 0 0
Endoplasmic Reticulum 0.0053 0.0048 0.0058 0.0011 0.0029 0.0029 0.0103 0.0072 0.009 0.0039 0.0034 0.0057 0.0084 0.0049 0.0059 0.0018 0.018 0.0049
Endosome 0.2262 0.1412 0.1774 0.2785 0.2429 0.1843 0.2473 0.1866 0.2368 0.2885 0.2367 0.2464 0.1449 0.1699 0.1973 0.1551 0.3614 0.195
Golgi 0.0057 0.0015 0.0023 0.0059 0.0062 0.0014 0.0103 0.0029 0.0058 0.0068 0.0059 0.0035 0.0063 0.0013 0.0249 0.0232 0.0114 0.0076
Lipid Particles 0.0147 0.0035 0.0065 0.0043 0.0113 0.0034 0.0226 0.0033 0.0059 0.0062 0.007 0.0064 0.0082 0.0055 0.0097 0.0117 0.0246 0.0095
Mitochondria 0.0054 0.0021 0.0073 0.0034 0.0036 0.0073 0.0196 0.0045 0.0062 0.0656 0.0343 0.0045 0.01 0.001 0.0097 0.0114 0.0023 0.0351
None 0.362 0.3597 0.407 0.3294 0.2985 0.2974 0.1127 0.1183 0.1374 0.0772 0.0879 0.0542 0.3026 0.2497 0.2762 0.2466 0.053 0.1553
Nuclear Periphery 0.0053 0.0042 0.0056 0.0014 0.006 0.0029 0.0205 0.0064 0.0119 0.0109 0.0091 0.0075 0.0105 0.0045 0.0066 0.0022 0.0303 0.0051
Nucleolus 0.0034 0.0012 0.0014 0.001 0.0018 0.0005 0.0035 0.0025 0.0015 0.0008 0.0027 0.0008 0.0007 0.0013 0.0014 0.0011 0.0005 0.0003
Nucleus 0.0121 0.0087 0.0044 0.0021 0.0042 0.0038 0.0187 0.0112 0.014 0.0055 0.0085 0.0061 0.004 0.0033 0.0058 0.0043 0.0047 0.0029
Peroxisomes 0.0044 0.0006 0.0019 0.0008 0.0033 0.0005 0.0049 0.0012 0.0042 0.0033 0.0025 0.0018 0.0059 0.0006 0.0042 0.0032 0.0148 0.0044
Punctate Nuclear 0.0027 0.0006 0.0008 0.0004 0.0037 0.0009 0.0057 0.0016 0.0032 0.0172 0.0037 0.0022 0.0012 0.0019 0.0022 0.0026 0.0024 0.0015
Vacuole 0.1342 0.2234 0.1258 0.2302 0.1848 0.1941 0.2215 0.2868 0.1917 0.2212 0.2819 0.2704 0.1414 0.2198 0.1518 0.2535 0.1842 0.1693
Vacuole Periphery 0.0128 0.022 0.0172 0.0261 0.0335 0.0313 0.0303 0.0293 0.0274 0.0413 0.0318 0.0375 0.0139 0.0223 0.0215 0.0188 0.0313 0.0205

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 0.8265 0.7389 2.1931 2.6732 3.7507 1.6521 2.6147 3.7816 5.5271 3.7351
Translational Efficiency – – – – – – – – – –

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency –
Standard name
Human Ortholog
Description Integral membrane component of ER-derived COPII-coated vesicles; functions in ER to Golgi transport; EMP46 has a paralog, EMP47, that arose from the whole genome duplication
Localization
Cell Percentages vacuole (32%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Emp46

Emp46


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Emp46-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available