Standard name | |
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Human Ortholog | |
Description | Protein of unknown function; overexpression suppresses the lethality caused by a rad53 null mutation |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 1 | 2 |
Bud | 1 | 0 | 1 | 4 | 1 | 2 | 12 | 5 | 9 | 11 | 7 | 12 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 2 | 3 | 5 | 9 | 9 |
Bud Neck | 0 | 0 | 1 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 1 | 0 | 1 | 0 | 1 | 2 | 3 | 7 |
Bud Site | 0 | 0 | 0 | 0 | 2 | 5 | 4 | 13 | 5 | 15 | 6 | 18 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 50 | 27 | 66 | 35 | 54 | 58 | 56 | 51 | 24 | 26 | 11 | 20 | 60 | 162 | 191 | 115 | 120 | 117 | 46 | 15 | 41 | 82 | 90 | 115 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 1 | 0 | 21 | 16 | 13 | 0 | 0 | 2 | 0 | 0 | 3 |
Endosome | 0 | 0 | 44 | 10 | 22 | 13 | 8 | 4 | 1 | 2 | 0 | 0 | 5 | 59 | 58 | 19 | 13 | 13 | 2 | 2 | 17 | 7 | 18 | 17 |
Golgi | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 2 | 0 |
Mitochondria | 212 | 26 | 78 | 95 | 89 | 264 | 402 | 393 | 346 | 455 | 357 | 442 | 1 | 2 | 3 | 10 | 5 | 12 | 13 | 4 | 41 | 11 | 22 | 46 |
Nucleus | 84 | 37 | 91 | 105 | 124 | 146 | 192 | 242 | 265 | 320 | 214 | 280 | 130 | 134 | 84 | 32 | 22 | 12 | 153 | 53 | 151 | 149 | 152 | 123 |
Nuclear Periphery | 0 | 0 | 0 | 1 | 0 | 1 | 3 | 1 | 2 | 1 | 3 | 9 | 0 | 0 | 3 | 0 | 0 | 0 | 0 | 0 | 3 | 1 | 2 | 1 |
Nucleolus | 0 | 0 | 0 | 0 | 1 | 1 | 8 | 7 | 8 | 15 | 18 | 17 | 0 | 1 | 0 | 0 | 0 | 0 | 2 | 0 | 7 | 5 | 10 | 9 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 1 | 0 | 9 | 7 | 6 | 1 | 4 | 7 | 8 | 4 | 0 | 3 | 3 | 6 | 6 | 0 | 1 | 4 | 18 | 8 | 30 | 21 | 35 | 46 |
Vac/Vac Membrane | 26 | 34 | 68 | 39 | 39 | 45 | 33 | 20 | 19 | 28 | 12 | 18 | 13 | 77 | 73 | 57 | 78 | 67 | 98 | 34 | 120 | 90 | 141 | 145 |
Unique Cell Count | 299 | 105 | 307 | 234 | 289 | 399 | 538 | 540 | 490 | 608 | 415 | 549 | 206 | 412 | 381 | 220 | 227 | 207 | 357 | 125 | 441 | 389 | 506 | 559 |
Labelled Cell Count | 374 | 124 | 358 | 298 | 338 | 537 | 723 | 743 | 687 | 878 | 629 | 819 | 213 | 443 | 421 | 254 | 256 | 242 | 357 | 125 | 441 | 389 | 506 | 559 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 3.2 | 4.2 | 4.1 | 3.9 | 4.5 | 3.7 | 3.7 | 3.3 | 3.4 | 3.4 | 3.0 | 3.1 | 5.4 | 5.5 | 5.1 | 5.5 | 5.1 | 5.1 | 5.3 | 5.6 | 5.7 |
Std Deviation (1e-4) | 0.3 | 0.5 | 1.3 | 1.6 | 3.0 | 1.8 | 1.7 | 1.1 | 1.0 | 0.9 | 0.9 | 0.7 | 1.1 | 1.0 | 0.9 | 1.3 | 1.1 | 1.7 | 1.0 | 1.5 | 1.5 |
Intensity Change (Log2) | – | – | – | -0.07 | 0.13 | -0.17 | -0.17 | -0.3 | -0.26 | -0.3 | -0.45 | -0.4 | 0.39 | 0.4 | 0.3 | 0.41 | 0.3 | 0.31 | 0.37 | 0.43 | 0.47 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | 1.0 | 3.1 | 2.6 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | -0.9613 | -0.4233 | -0.6644 | -0.7093 | -1.1222 | -0.4572 | 0.3025 | 0.3263 | 0.4882 | 0.3196 | 0.9425 | 0.4479 | 0.0397 | -0.507 | -0.5304 | -0.1899 | -0.7009 | -0.2915 |
Actin | 0.0015 | 0.0006 | 0.0004 | 0.0005 | 0.0002 | 0.0003 | 0.0203 | 0.0004 | 0.0068 | 0.0162 | 0.0325 | 0.0026 | 0.004 | 0.0052 | 0.015 | 0.0072 | 0.0001 | 0.0003 |
Bud | 0.0003 | 0.0026 | 0.0001 | 0.0001 | 0 | 0.0002 | 0.0006 | 0.0002 | 0.0004 | 0.0003 | 0.0005 | 0.0001 | 0.0012 | 0.0003 | 0.0002 | 0.0001 | 0.0001 | 0.0001 |
Bud Neck | 0.0022 | 0.0008 | 0.0003 | 0.0012 | 0.0009 | 0.0029 | 0.0089 | 0.0009 | 0.0033 | 0.002 | 0.0021 | 0.0023 | 0.0152 | 0.001 | 0.0005 | 0.0002 | 0.001 | 0.0021 |
Bud Periphery | 0.0007 | 0.0006 | 0.0001 | 0.0001 | 0 | 0.0001 | 0.0009 | 0.0002 | 0.0006 | 0.0005 | 0.0008 | 0.0002 | 0.0019 | 0.0003 | 0.0002 | 0.0004 | 0.0001 | 0.0001 |
Bud Site | 0.0015 | 0.0008 | 0.0002 | 0.0002 | 0.0001 | 0.0002 | 0.0033 | 0.0013 | 0.0114 | 0.0016 | 0.0014 | 0.0002 | 0.0156 | 0.0082 | 0.0012 | 0.0003 | 0.0005 | 0.0005 |
Cell Periphery | 0.0001 | 0.0001 | 0 | 0 | 0 | 0 | 0.0002 | 0 | 0.0003 | 0.0001 | 0.0002 | 0.0001 | 0.0006 | 0.0001 | 0 | 0.0001 | 0.0001 | 0 |
Cytoplasm | 0.055 | 0.0806 | 0.0405 | 0.0493 | 0.0254 | 0.0401 | 0.0459 | 0.0722 | 0.0405 | 0.027 | 0.0633 | 0.0434 | 0.0633 | 0.1001 | 0.0572 | 0.0218 | 0.0122 | 0.0461 |
Cytoplasmic Foci | 0.0032 | 0.0032 | 0.0023 | 0.0024 | 0.0024 | 0.0014 | 0.0076 | 0.0037 | 0.0059 | 0.0057 | 0.0274 | 0.0026 | 0.0279 | 0.0062 | 0.003 | 0.0014 | 0.0032 | 0.004 |
Eisosomes | 0.0002 | 0.0001 | 0.0001 | 0 | 0 | 0 | 0.0002 | 0 | 0.0001 | 0.0002 | 0.0005 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.0003 | 0 | 0 |
Endoplasmic Reticulum | 0.0021 | 0.0034 | 0.0013 | 0.0025 | 0.0004 | 0.0008 | 0.0029 | 0.0021 | 0.004 | 0.0016 | 0.0028 | 0.0015 | 0.0059 | 0.0042 | 0.003 | 0.0013 | 0.0005 | 0.0017 |
Endosome | 0.0039 | 0.0042 | 0.0014 | 0.0021 | 0.0015 | 0.0019 | 0.0093 | 0.0023 | 0.0072 | 0.0164 | 0.011 | 0.0033 | 0.0328 | 0.012 | 0.0098 | 0.0075 | 0.06 | 0.0148 |
Golgi | 0.0005 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0023 | 0.0004 | 0.0027 | 0.004 | 0.005 | 0.0005 | 0.0064 | 0.0059 | 0.0035 | 0.0079 | 0.0056 | 0.0014 |
Lipid Particles | 0.0007 | 0.0005 | 0.0003 | 0.0001 | 0.0006 | 0.0002 | 0.0086 | 0.0004 | 0.008 | 0.0143 | 0.0164 | 0.0012 | 0.0243 | 0.0019 | 0.005 | 0.0191 | 0.0019 | 0.0022 |
Mitochondria | 0.0006 | 0.0016 | 0.0005 | 0.001 | 0.0004 | 0.0005 | 0.0034 | 0.0041 | 0.0027 | 0.004 | 0.0068 | 0.0055 | 0.0213 | 0.0069 | 0.0021 | 0.0413 | 0.0008 | 0.0008 |
None | 0.1826 | 0.2345 | 0.1714 | 0.0663 | 0.1883 | 0.0803 | 0.1132 | 0.0775 | 0.0441 | 0.1433 | 0.1127 | 0.0643 | 0.0725 | 0.0928 | 0.0854 | 0.1055 | 0.0009 | 0.0433 |
Nuclear Periphery | 0.0256 | 0.0366 | 0.0311 | 0.0256 | 0.0473 | 0.021 | 0.0242 | 0.0204 | 0.023 | 0.0278 | 0.0286 | 0.0157 | 0.058 | 0.0272 | 0.0332 | 0.013 | 0.0054 | 0.0126 |
Nucleolus | 0.007 | 0.0102 | 0.0085 | 0.0106 | 0.0118 | 0.0179 | 0.0084 | 0.0062 | 0.0103 | 0.0078 | 0.0122 | 0.0113 | 0.0113 | 0.0044 | 0.0063 | 0.003 | 0.0134 | 0.0127 |
Nucleus | 0.6411 | 0.5409 | 0.6622 | 0.735 | 0.6323 | 0.7497 | 0.6761 | 0.7487 | 0.7703 | 0.6559 | 0.5909 | 0.7676 | 0.5728 | 0.6951 | 0.7082 | 0.75 | 0.8755 | 0.8019 |
Peroxisomes | 0.0004 | 0.0002 | 0.0002 | 0.0002 | 0.0001 | 0.0001 | 0.0053 | 0.002 | 0.0088 | 0.0071 | 0.0034 | 0.0003 | 0.0058 | 0.0023 | 0.0011 | 0.0026 | 0.0001 | 0.0006 |
Punctate Nuclear | 0.069 | 0.0755 | 0.0783 | 0.1 | 0.0878 | 0.0814 | 0.0557 | 0.0553 | 0.0463 | 0.0611 | 0.0766 | 0.0757 | 0.0446 | 0.022 | 0.0614 | 0.0147 | 0.0137 | 0.0467 |
Vacuole | 0.0014 | 0.0021 | 0.0005 | 0.002 | 0.0003 | 0.0008 | 0.002 | 0.0014 | 0.0025 | 0.002 | 0.0037 | 0.001 | 0.0096 | 0.0032 | 0.0024 | 0.0009 | 0.0042 | 0.0042 |
Vacuole Periphery | 0.0004 | 0.0006 | 0.0003 | 0.0006 | 0.0003 | 0.0002 | 0.0007 | 0.0003 | 0.0007 | 0.0011 | 0.0012 | 0.0006 | 0.0048 | 0.0008 | 0.0012 | 0.0013 | 0.0008 | 0.004 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 5.1473 | 1.6732 | 4.9665 | 5.8856 | 8.4939 | 7.4829 | 6.7673 | 4.8529 | 6.4074 | 8.8566 |
Translational Efficiency | – | – | – | – | – | – | – | – | – | – |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | – |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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