Standard name
Human Ortholog
Description N-glycosylated protein; involved in the maintenance of bud site selection during bipolar budding; localization requires Rax1p; RAX2 mRNA stability is regulated by Mpt5p
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.53 0.62 0.75 0.62 0.69 0.66 0.62 0.52 0.54 0.5 0.51 0.38 0.64 0.59 0.58 0.49 0.5 0.48 0.25 0.21 0.22 0.27 0.33 0.27
Bud 0.23 0.17 0.3 0.22 0.38 0.34 0.25 0.31 0.28 0.26 0.26 0.24 0.22 0.26 0.31 0.2 0.19 0.22 0.08 0.06 0.08 0.14 0.12 0.1
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.21 0.29 0.31 0.28 0.22 0.2
Bud Site 0.21 0.25 0.25 0.34 0.21 0.27 0.24 0.16 0.24 0.21 0.22 0.22 0.39 0.29 0.27 0.12 0.05 0.11 – – – – – –
Cell Periphery 0.18 0.16 0.14 0.09 0.08 0.07 0.1 0.08 0.1 0.1 0.11 0.1 0.06 0.12 0.13 0.11 0.17 0.17 0.18 0.12 0.13 0.06 0.09 0.08
Cytoplasm 0.06 0.11 0.1 0.11 0.16 0.16 0.2 0.17 0.14 0.13 0.18 0.16 0.07 0.07 0.09 0.25 0.15 0.12 0 0 0 0 0 0
Endoplasmic Reticulum 0.07 0.08 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0.17 0.09 0.09 0.13 0.13 0.18
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0
Mitochondria 0 0.1 0.08 0.12 0.06 0.11 0.13 0.26 0.32 0.38 0.34 0.46 0.11 0.07 0.13 0.09 0.07 0.08 0 0.08 0 0 0 0
Nucleus 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0.07 0.07 0.05 0 0.08 0.11 0.06 0.1 0.08 0.1 0.12 0.11 0.16 0.18 0 0 0.06 0 0 0 0 0 0
SpindlePole 0 0 0 0.13 0 0 0.05 0 0.05 0.05 0 0 0 0.05 0.06 0.08 0.06 0.08 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0.09 0.1 0.1 0.07 0.12
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 141 63 172 129 164 189 245 244 229 206 144 118 123 159 179 187 135 177 100 33 57 43 86 75
Bud 61 17 69 46 90 96 99 148 119 107 74 74 43 70 95 77 52 79 33 9 21 21 32 28
Bud Neck 1 0 2 1 6 3 2 3 7 4 4 4 0 0 1 2 6 1 87 46 81 43 58 54
Bud Site 57 25 58 72 50 77 95 76 102 88 62 67 75 79 84 45 13 41 – – – – – –
Cell Periphery 47 16 33 18 19 21 40 36 41 43 30 32 12 33 39 43 47 63 75 18 34 9 23 21
Cytoplasm 17 11 22 24 38 46 78 81 60 53 51 50 13 20 27 94 40 44 3 1 1 0 1 2
Endoplasmic Reticulum 18 8 6 6 5 6 13 12 5 9 4 3 5 11 15 15 11 16 67 14 24 19 34 51
Endosome 1 0 0 0 0 1 1 1 0 1 0 0 0 9 4 0 2 3 3 1 2 0 3 3
Golgi 6 0 0 0 0 0 0 0 0 0 0 0 0 0 0 10 16 10 2 0 0 0 0 0
Mitochondria 2 10 18 26 15 31 52 122 135 157 97 142 22 18 41 34 18 30 3 12 11 0 2 3
Nucleus 17 0 2 8 1 6 12 6 10 4 6 9 4 6 12 22 9 11 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 2 0 2 2 1 0 1 1 0 0 0 1 1 1 0 0 1
Nucleolus 1 0 0 3 0 0 3 5 2 1 0 2 0 0 2 1 0 3 0 0 0 0 0 0
Peroxisomes 9 7 16 10 5 23 45 26 44 32 28 38 22 43 57 3 10 22 0 0 0 1 0 0
SpindlePole 8 0 8 27 4 10 20 12 20 20 12 6 6 14 20 32 16 28 0 0 0 0 0 0
Vac/Vac Membrane 1 2 0 0 2 0 0 1 1 0 0 2 0 0 0 4 5 7 27 14 26 15 17 33
Unique Cell Count 268 102 229 209 238 286 394 471 421 413 283 308 192 271 309 381 270 366 410 159 264 158 263 278
Labelled Cell Count 387 159 406 370 399 509 705 775 775 727 514 548 325 463 577 569 380 535 410 159 264 158 263 278


Bud Neck, Cell Periphery

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 13.0 10.0 7.6 6.9 7.0 6.6 6.2 5.8 5.6 5.3 5.2 4.9 8.7 8.1 6.9 11.3 13.8 13.7 11.0 10.1 10.3
Std Deviation (1e-4) 2.4 2.2 1.4 1.2 1.2 1.3 1.3 1.1 1.4 1.2 1.2 0.9 1.5 1.3 1.1 2.3 2.8 2.7 2.0 1.9 1.7
Intensity Change (Log2) – – – -0.13 -0.13 -0.2 -0.28 -0.39 -0.44 -0.51 -0.54 -0.64 0.19 0.09 -0.14 0.57 0.86 0.85 0.53 0.4 0.44


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches -3.0 -1.5 -2.2 -3.3 -5.9 -5.2 -6.2 -5.6 -8.5 -2.5 -3.9 -4.1 -6.3 -5.7 -6.4
Bud -1.9 1.8 0.8 -1.4 0.3 -0.5 -1.1 -1.0 -1.6 -1.8 -1.1 0.2 -2.8 -2.8 -2.3
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 2.1 -1.1 0.4 -0.3 -2.9 -0.3 -1.2 -0.9 -1.0 3.0 1.0 0.5 -4.3 -6.5 -4.5
Cell Periphery -1.9 -2.2 -2.6 -1.6 -2.8 -1.8 -1.5 -1.3 -1.4 -2.7 -0.7 -0.6 -1.1 0.9 0.9
Cytoplasm 0.6 2.1 2.2 3.3 2.7 1.7 1.2 2.7 2.2 -1.0 -0.9 -0.3 4.6 1.8 0.9
Endoplasmic Reticulum 0.2 -0.4 -0.4 0.5 -0.1 0 -0.4 0 0 0 0.9 1.3 0.9 0.9 1.1
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 3.7 0
Mitochondria 1.6 -0.7 1.1 2.0 5.6 6.9 8.2 7.1 9.6 1.3 -0.5 2.0 0.5 -0.5 0.1
Nucleus 0 0 0 1.8 0 0 0 0 0 0 0 2.2 3.0 1.9 1.7
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes -1.0 -2.5 0.4 1.8 -0.8 1.5 0.4 1.2 2.0 1.6 3.1 3.8 -4.3 -1.6 -0.5
SpindlePole 3.6 -1.2 0 0.9 -0.7 0.8 0.8 0.4 -1.1 -0.2 0.9 1.5 2.4 1.3 2.1
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 8.4019 10.307 9.3645 8.8891 9.5292 9.9045 7.8305 11.0317 10.1176 10.7077 10.0438 11.5222 6.9696 9.4382 8.1129 8.0872 8.8638 9.2197
Actin 0.0885 0.0568 0.1306 0.0899 0.1714 0.0826 0.0759 0.0481 0.0824 0.0235 0.1124 0.0421 0.1271 0.105 0.1023 0.1048 0.1731 0.0674
Bud 0.0063 0.0046 0.0159 0.0082 0.0085 0.0042 0.0065 0.0059 0.0154 0.0102 0.0124 0.0043 0.0048 0.0095 0.0193 0.0096 0.0052 0.0038
Bud Neck 0.2275 0.0241 0.2485 0.408 0.4637 0.7903 0.1387 0.0464 0.1865 0.2688 0.1609 0.6532 0.2099 0.0719 0.2432 0.3397 0.2821 0.6952
Bud Periphery 0.021 0.0079 0.0566 0.0721 0.0482 0.0224 0.0203 0.0151 0.061 0.0293 0.0625 0.0243 0.0135 0.0182 0.0784 0.0992 0.0427 0.0264
Bud Site 0.4574 0.7693 0.4172 0.1136 0.0896 0.0171 0.2009 0.5414 0.1808 0.0245 0.046 0.0141 0.3592 0.6055 0.2854 0.0296 0.0656 0.0216
Cell Periphery 0.119 0.0359 0.0623 0.1087 0.0627 0.0315 0.0963 0.0788 0.1084 0.0447 0.1074 0.0471 0.1147 0.0493 0.0967 0.1587 0.1273 0.0606
Cytoplasm 0.033 0.065 0.0305 0.0892 0.0357 0.0093 0.0442 0.0499 0.0418 0.0467 0.0968 0.0151 0.0251 0.0631 0.0554 0.0566 0.0342 0.0166
Cytoplasmic Foci 0.0118 0.0034 0.0052 0.012 0.0056 0.006 0.0803 0.0068 0.018 0.026 0.0262 0.0063 0.0167 0.0103 0.0127 0.0077 0.0138 0.0077
Eisosomes 0.0018 0.0005 0.0015 0.0058 0.0019 0.0017 0.0013 0.0031 0.0012 0.0002 0.0015 0.0006 0.0026 0.001 0.001 0.0031 0.0051 0.0014
Endoplasmic Reticulum 0.0016 0.0033 0.0036 0.0025 0.058 0.0023 0.0114 0.0195 0.02 0.0252 0.0293 0.0268 0.0076 0.0048 0.0079 0.0105 0.0217 0.012
Endosome 0.0047 0.0034 0.0048 0.0167 0.0085 0.0058 0.0799 0.018 0.0569 0.1255 0.0618 0.0293 0.0107 0.0076 0.0267 0.0377 0.0415 0.012
Golgi 0.0022 0.0052 0.0071 0.0336 0.0154 0.0118 0.032 0.0067 0.0318 0.0642 0.0538 0.0277 0.0144 0.0169 0.0176 0.0188 0.0735 0.0284
Lipid Particles 0.0063 0.0021 0.003 0.011 0.0145 0.004 0.0379 0.0183 0.0158 0.0318 0.0363 0.0136 0.0143 0.0082 0.0142 0.0457 0.0141 0.0108
Mitochondria 0.0019 0.0005 0.0015 0.0019 0.0012 0.0008 0.0155 0.0011 0.0163 0.0054 0.0062 0.0027 0.0128 0.0058 0.0017 0.0016 0.004 0.004
None 0.0012 0.0002 0.0003 0.0002 0.0003 0.0001 0.0081 0.0007 0.0006 0.0003 0.0004 0.0004 0.0017 0.0003 0.0005 0.0212 0.0002 0.0001
Nuclear Periphery 0.0005 0.0003 0.0004 0.0004 0.001 0.0002 0.016 0.0024 0.0023 0.0043 0.0029 0.0022 0.0017 0.0007 0.0012 0.0009 0.0013 0.0006
Nucleolus 0.001 0.0044 0.0009 0.002 0.0007 0.0005 0.0034 0.002 0.0025 0.0028 0.0011 0.0011 0.0014 0.0007 0.0015 0.0018 0.0011 0.0008
Nucleus 0.001 0.0023 0.0011 0.0021 0.0011 0.0006 0.0205 0.0031 0.0033 0.0035 0.0022 0.0021 0.0024 0.0011 0.0018 0.0019 0.0013 0.0012
Peroxisomes 0.0036 0.0003 0.0009 0.0011 0.0007 0.0013 0.0177 0.0005 0.001 0.0013 0.0022 0.0005 0.0092 0.001 0.0017 0.0006 0.0018 0.0007
Punctate Nuclear 0.0008 0.0002 0.0003 0.0003 0.0004 0.0002 0.018 0.0005 0.0006 0.0009 0.0005 0.0004 0.0012 0.0004 0.0004 0.0005 0.0003 0.0002
Vacuole 0.0088 0.0101 0.0074 0.0198 0.0101 0.0067 0.0675 0.1293 0.148 0.2466 0.1707 0.0818 0.0478 0.0179 0.0293 0.0477 0.0848 0.0262
Vacuole Periphery 0.0002 0.0002 0.0004 0.0009 0.0007 0.0004 0.0077 0.0023 0.0056 0.0142 0.0065 0.0044 0.0011 0.0005 0.0012 0.002 0.0053 0.0021

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 25.1527 25.851 31.7711 43.2193 20.309 19.5691 39.411 45.2978 30.5515 35.3128
Translational Efficiency 0.6565 0.7365 0.7554 0.4358 0.7519 0.8983 0.5531 0.4975 0.6021 0.5004

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✔
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description N-glycosylated protein; involved in the maintenance of bud site selection during bipolar budding; localization requires Rax1p; RAX2 mRNA stability is regulated by Mpt5p
Localization
Cell Percentages cell periphery (72%)
Cell Cycle Regulation No
Subcompartmental Group cell periphery-3

Rax2

Rax2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Rax2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available