Standard name
Human Ortholog
Description Actin-related protein that binds nucleosomes; a component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0.06 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Cytoplasm 0.9 0.83 0.74 0.85 0.81 0.77 0.77 0.63 0.62 0.65 0.45 0.48 0.83 0.93 0.9 – – – 0.64 0.6 0.49 0.52 0.58 0.57
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 – – – 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Mitochondria 0.05 0.08 0.27 0.22 0.27 0.33 0.33 0.49 0.56 0.45 0.66 0.65 0 0 0 – – – 0.1 0.07 0.18 0.09 0.1 0.06
Nucleus 0 0.05 0 0 0 0 0 0.06 0.05 0.09 0.11 0.09 0.09 0 0 – – – 0 0.09 0 0 0 0.07
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0.06 0 0
Vac/Vac Membrane 0.06 0.1 0 0 0 0 0 0 0 0 0 0 0 0 0.07 – – – 0.13 0.13 0.08 0.1 0.13 0.14
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 3 1 1 1
Bud 1 0 0 1 2 2 2 2 4 4 3 7 0 0 0 – – – 2 3 1 8 3 4
Bud Neck 1 0 0 0 0 1 2 3 2 3 0 1 0 0 1 – – – 0 0 0 0 2 1
Bud Site 0 0 0 0 0 2 2 2 4 5 4 2 0 0 0 – – – – – – – – –
Cell Periphery 0 1 0 0 0 0 0 0 0 0 1 1 0 0 0 – – – 0 0 0 0 0 1
Cytoplasm 114 95 73 126 146 177 177 175 164 194 88 144 92 166 197 – – – 93 89 47 81 128 113
Endoplasmic Reticulum 0 1 1 0 0 0 1 1 0 0 0 2 0 2 1 – – – 0 1 1 2 1 2
Endosome 1 0 0 1 0 0 0 0 0 2 1 0 5 1 5 – – – 3 0 2 5 5 1
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 – – – 0 0 0 0 0 1
Mitochondria 6 9 27 32 48 77 77 135 147 136 128 196 0 0 1 – – – 14 10 17 14 22 11
Nucleus 2 6 1 6 4 7 7 18 14 27 21 26 10 7 5 – – – 4 12 3 5 6 14
Nuclear Periphery 0 0 0 0 0 0 1 1 0 0 1 0 0 0 0 – – – 0 1 0 0 0 1
Nucleolus 0 0 0 0 0 0 0 1 1 0 1 3 0 0 0 – – – 0 0 0 1 1 2
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 – – – 0 0 0 1 0 0
SpindlePole 0 0 1 1 1 3 5 7 5 11 2 8 2 0 1 – – – 2 4 3 9 8 6
Vac/Vac Membrane 8 11 2 2 8 1 0 0 2 0 2 2 4 6 16 – – – 18 18 7 15 28 28
Unique Cell Count 127 114 99 148 180 231 230 277 263 300 195 301 111 178 220 146 148 96 156 220 198
Labelled Cell Count 134 124 105 169 209 270 274 345 343 382 252 392 114 183 227 146 148 96 156 220 198


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.8 4.1 2.9 3.2 3.2 3.1 3.1 2.9 2.8 2.7 2.8 2.6 5.0 5.2 5.5 – – – 5.5 5.4 5.6
Std Deviation (1e-4) 0.9 0.9 0.6 1.7 1.7 1.7 1.7 1.6 1.8 1.5 1.8 1.3 1.4 2.6 2.4 – – – 2.2 2.3 2.2
Intensity Change (Log2) – – – 0.13 0.15 0.1 0.1 0.0 -0.09 -0.12 -0.06 -0.15 0.77 0.82 0.89 – – – 0.91 0.89 0.93


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -0.474 -0.2709 -0.3139 -0.4878 -0.3561 -0.059 0.8688 0.9766 0.9102 0.6457 0.7659 0.9117 -1.4437 -1.57 -1.2954 -1.823 -2.895 -2.2798
Actin 0.0017 0.0003 0.0005 0.0002 0.0001 0.001 0.029 0.0097 0.0047 0.0003 0.0004 0.0029 0 0.0002 0.0975 0.0102 0.0002 0.03
Bud 0.0008 0.0025 0.0025 0.001 0.0004 0.0047 0.0005 0.0011 0.0026 0.0001 0.0001 0.0004 0 0 0.0044 0 0 0.0003
Bud Neck 0.0008 0.0004 0.0009 0.0005 0.0002 0.0066 0.0023 0.0002 0.0018 0.0004 0.0001 0.002 0 0.0036 0.0003 0 0.0001 0.0015
Bud Periphery 0.0009 0.0011 0.0013 0.0004 0.0004 0.0024 0.0009 0.0012 0.0022 0.0001 0 0.001 0 0 0.0054 0.0001 0 0.0005
Bud Site 0.0038 0.009 0.0106 0.0003 0.0001 0.0009 0.0018 0.0128 0.0067 0.0002 0.0001 0.0004 0.0001 0.0004 0.0243 0.0002 0 0.0003
Cell Periphery 0.0001 0.0001 0.0003 0.0001 0 0.0002 0.0003 0.0002 0.0005 0.0001 0.0001 0.0001 0 0.0005 0.0002 0.0001 0.0001 0.0001
Cytoplasm 0.0703 0.0611 0.0585 0.0484 0.0611 0.1304 0.0553 0.0616 0.0851 0.0928 0.1224 0.1365 0.0063 0.0586 0.0707 0.0768 0.3741 0.0252
Cytoplasmic Foci 0.0236 0.007 0.0178 0.0107 0.0117 0.0242 0.0333 0.0094 0.0105 0.0092 0.0131 0.0268 0.1119 0.0177 0.0136 0.0112 0.0095 0.0137
Eisosomes 0.0009 0.0001 0.0002 0.0001 0 0.0001 0.0032 0.0002 0.0003 0.0001 0.0001 0.0001 0 0.0001 0.0009 0.0002 0 0.0001
Endoplasmic Reticulum 0.0008 0.0015 0.0009 0.0007 0.0004 0.0013 0.0053 0.0016 0.0013 0.0023 0.0014 0.0013 0.0004 0.0041 0.0011 0.0009 0.0206 0.0041
Endosome 0.0071 0.0028 0.0117 0.0131 0.0266 0.0208 0.0262 0.007 0.0045 0.0254 0.0484 0.0232 0.3468 0.0234 0.0021 0.0008 0.0056 0.0185
Golgi 0.0003 0.0002 0.0002 0.0001 0.0001 0.0003 0.0064 0.0005 0.001 0.0002 0.0003 0.0011 0.008 0.0015 0.0039 0.0009 0.0001 0.0064
Lipid Particles 0.0017 0.0007 0.0016 0.0004 0.0002 0.0006 0.0103 0.0012 0.0021 0.0008 0.001 0.0042 0.0194 0.0332 0.0039 0.0018 0.0018 0.0948
Mitochondria 0.0012 0.0008 0.0011 0.0007 0.0003 0.0008 0.0058 0.0061 0.0014 0.001 0.0004 0.0047 0.0001 0.0005 0.0134 0.0007 0.0001 0.0017
None 0.7691 0.8193 0.789 0.8714 0.8599 0.6769 0.7254 0.849 0.809 0.7207 0.7834 0.6759 0.4988 0.8151 0.7482 0.8037 0.578 0.7547
Nuclear Periphery 0.0019 0.0037 0.0026 0.0017 0.0005 0.0023 0.006 0.0023 0.0022 0.0031 0.002 0.0017 0.0002 0.0056 0.0007 0.0004 0.0038 0.0192
Nucleolus 0.0027 0.0075 0.0023 0.0015 0.0007 0.0031 0.0043 0.0009 0.0011 0.0016 0.0002 0.0013 0.0001 0.0099 0.0003 0.0002 0.0001 0.0004
Nucleus 0.0915 0.0472 0.0711 0.0205 0.0023 0.0754 0.0488 0.0143 0.0499 0.1198 0.0034 0.0901 0.0001 0.0024 0.0003 0.0003 0.002 0.0037
Peroxisomes 0.0007 0.0004 0.0004 0.0002 0.0001 0.0003 0.0127 0.0007 0.0011 0.0002 0.0003 0.0051 0.0017 0.0029 0.0066 0.0903 0.0001 0.0104
Punctate Nuclear 0.0157 0.032 0.0177 0.0175 0.0043 0.0309 0.017 0.0137 0.0072 0.0101 0.0034 0.0123 0.0003 0.0047 0.0015 0.0004 0.0025 0.0135
Vacuole 0.0039 0.0019 0.0077 0.0087 0.0289 0.0156 0.0042 0.005 0.0043 0.0098 0.0173 0.0081 0.0052 0.0147 0.0005 0.0008 0.0012 0.0005
Vacuole Periphery 0.0005 0.0004 0.0009 0.0018 0.0016 0.0014 0.0011 0.0014 0.0005 0.0016 0.0022 0.0009 0.0006 0.0008 0.0002 0.0001 0.0001 0.0004

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 11.3105 10.4854 16.6727 15.167 17.3803 5.0241 15.1454 20.1887 14.5402 15.9017
Translational Efficiency 1.1345 0.9383 0.6867 0.8273 0.6652 2.1819 0.7633 0.5371 0.5887 0.5341

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘
Standard name
Human Ortholog
Description Actin-related protein that binds nucleosomes; a component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A
Localization
Cell Percentages cytoplasm (19%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Arp6

Arp6


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Arp6-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available