Standard name
Human Ortholog
Description 3-keto sterol reductase; catalyzes the last of three steps required to remove two C-4 methyl groups from an intermediate in ergosterol biosynthesis; mutants are sterol auxotrophs; mutation is functionally complemented by human HSD17B7
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.08 0.13 0.22 0.17 0.24 0.11 0 0.07 0 0 0 0 0.12 0.05 0 0.08 0.1 0.13 0.19 0.2 0.18 0.14 0.15 0.12
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0.05 0 0.07 0 0 0 0 0 0 0 0.05 0 0.05 0.05 0 0 0 0 0 0 0
Endoplasmic Reticulum 0 0.05 0.06 0 0.14 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0 0 0
Endosome 0.11 0 0.05 0.06 0.09 0.06 0.07 0 0 0 0 0 0.09 0.07 0.08 0 0 0 0.17 0.25 0.18 0.15 0.14 0.14
Golgi 0.07 0.09 0.47 0.34 0.63 0.35 0.1 0.08 0 0 0 0 0.26 0.28 0.39 0.74 0.75 0.66 0.27 0.29 0.28 0.29 0.23 0.26
Mitochondria 0.73 0.77 0.43 0.48 0.23 0.47 0.73 0.7 0.8 0.76 0.84 0.78 0.35 0.11 0.1 0.1 0.09 0.08 0 0 0 0 0 0.05
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0.05 0.09 0.06 0.05 0 0.08 0.06 0.07 0.07 0.07 0.08 0.07 0 0.09 0.05 0 0 0 0 0 0 0 0 0
Nucleolus 0.24 0.2 0.13 0.16 0.08 0.16 0.21 0.26 0.27 0.24 0.26 0.23 0.33 0.5 0.45 0.16 0.21 0.21 0 0 0 0 0 0
Peroxisomes 0.08 0 0.09 0.08 0 0 0 0 0 0 0 0 0.07 0 0 0 0 0 0.23 0.09 0.2 0.27 0.29 0.31
SpindlePole 0 0 0.05 0.07 0 0 0 0 0 0 0 0 0.1 0.12 0.1 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0.07 0 0 0.06 0.07 0 0.06 0 0 0 0.07 0 0 0 0.05 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 19 12 26 32 56 28 17 22 9 5 2 3 15 7 3 34 14 40 50 25 27 16 29 21
Bud 0 1 2 4 4 3 5 4 4 4 0 5 0 1 0 2 1 4 0 0 0 0 2 1
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 1 0
Bud Site 0 0 0 1 0 0 0 1 1 3 0 1 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 1 1 0 2 0 2 0 0 0 1 0 0 1 0 1 2 0 0 0 0 0 0 0
Cytoplasm 7 0 2 9 6 17 13 7 10 4 5 7 2 7 1 20 7 13 0 0 0 0 0 0
Endoplasmic Reticulum 1 5 7 0 32 15 8 0 2 1 0 0 1 4 2 10 6 14 9 1 10 2 5 1
Endosome 26 0 6 12 20 15 30 10 1 4 0 11 11 9 13 8 3 5 46 31 28 18 28 24
Golgi 17 8 56 64 145 90 44 26 5 10 0 3 33 36 60 303 106 206 72 36 43 34 43 46
Mitochondria 177 72 51 91 53 122 323 229 259 308 300 312 44 14 16 39 12 26 6 5 3 4 7 9
Nucleus 0 0 0 0 0 0 1 2 1 2 4 2 0 1 0 3 0 1 0 0 0 0 0 0
Nuclear Periphery 13 8 7 10 5 20 27 22 21 27 27 29 4 12 8 11 1 7 2 4 3 2 4 2
Nucleolus 57 19 15 30 19 42 93 86 87 96 94 93 42 64 70 65 29 65 1 1 0 1 0 0
Peroxisomes 19 2 11 16 2 0 6 3 5 8 0 4 9 4 6 2 0 4 62 11 30 31 56 55
SpindlePole 6 0 6 13 7 5 4 4 0 4 0 3 13 15 16 13 3 8 8 0 0 1 4 6
Vac/Vac Membrane 3 2 5 3 10 19 8 5 18 29 11 24 2 2 4 30 2 12 4 6 3 3 6 5
Unique Cell Count 241 94 119 191 229 259 442 327 323 405 358 398 127 129 155 410 141 313 267 127 154 120 195 179
Labelled Cell Count 345 130 195 285 361 376 581 421 423 505 444 497 176 177 199 541 186 405 267 127 154 120 195 179


Lipid Particles

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 13.7 17.9 15.0 14.7 15.8 14.8 14.5 15.6 13.9 14.7 13.5 14.1 17.8 18.8 18.8 21.8 24.7 23.6 14.7 15.4 15.8
Std Deviation (1e-4) 2.8 4.4 2.6 2.3 2.5 2.6 2.8 3.2 3.3 3.4 3.1 3.3 3.7 4.0 3.1 5.4 6.1 6.1 2.8 3.5 3.4
Intensity Change (Log2) – – – -0.03 0.08 -0.02 -0.05 0.05 -0.12 -0.04 -0.16 -0.09 0.25 0.33 0.32 0.54 0.72 0.65 -0.03 0.04 0.08


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches -1.1 0.5 -2.8 -6.6 -4.6 -6.6 -8.4 -8.6 -8.8 -2.1 -3.8 -5.3 -4.1 -2.7 -2.3
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 2.0 0 0 0 0 0 0 0 0 0 0 0 0
Endoplasmic Reticulum 0 2.3 0 0 0 0 0 0 0 0 -1.1 0 0 -0.6 -0.6
Endosome 0.5 1.2 0.3 0.7 0 0 0 0 0 1.1 0.6 1.1 0 0 0
Golgi -2.4 2.9 -2.3 -9.4 -9.4 -12.3 -12.9 -13.8 -13.9 -3.4 -3.1 -1.4 5.5 4.7 3.6
Mitochondria 0.8 -3.8 0.8 6.2 5.3 7.6 6.9 8.8 7.4 -1.3 -5.7 -6.2 -8.5 -6.4 -8.4
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery -0.2 0 0.6 0.1 0.3 0.2 0.3 0.6 0.5 -1.0 1.0 -0.3 0 0 0
Nucleolus 0.8 -1.3 0.9 2.1 3.1 3.2 2.6 3.1 2.5 3.8 6.2 5.8 0.9 1.7 2.0
Peroxisomes -0.3 0 0 0 0 0 0 0 0 -0.6 -2.0 -1.8 0 -3.7 0
SpindlePole 0.6 0 0 0 0 0 0 0 0 1.5 1.9 1.6 0 0 0
Vacuole 0 0.1 1.2 0 0 0.6 1.2 0 0.8 0 0 0 1.2 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 7.119 10.3679 9.3564 8.0656 8.4028 9.6533 7.8502 9.6932 8.7427 8.8421 7.7909 9.0553 7.3571 10.7697 9.1182 7.7607 7.3324 10.3102
Actin 0.0277 0.0027 0.0083 0.0039 0.0011 0.0132 0.0049 0.0054 0.0111 0.0006 0.0008 0.0008 0.0562 0.0016 0.0177 0.0282 0.0096 0.0243
Bud 0.0004 0.0002 0.0002 0.0001 0.0001 0.0001 0.0001 0 0.0001 0 0 0 0.0005 0 0.0003 0.0002 0.0001 0
Bud Neck 0.0007 0.0004 0.0013 0.0004 0.0001 0.0005 0.0007 0.0002 0.0003 0.0002 0.0001 0.0001 0.0032 0.0001 0.0015 0.0015 0.0003 0.0002
Bud Periphery 0.0006 0.0003 0.0003 0.0002 0.0001 0.0002 0.0001 0 0.0002 0.0001 0 0 0.0006 0 0.0005 0.0003 0.0002 0
Bud Site 0.0039 0.0013 0.0047 0.0006 0.0003 0.0003 0.0016 0.0001 0.0012 0.0001 0.0001 0.0001 0.0098 0.0001 0.0066 0.0008 0.0002 0.0001
Cell Periphery 0.0004 0.0005 0.0005 0.0002 0 0.0002 0.0002 0.0001 0.0001 0.0004 0.0001 0.0001 0.0005 0.0003 0.0003 0.0002 0.0002 0.0001
Cytoplasm 0.013 0.0006 0.0009 0.0003 0.0001 0.0008 0.0009 0.0001 0.0001 0.0001 0 0 0.0174 0.0001 0.0004 0.0008 0.0066 0
Cytoplasmic Foci 0.0674 0.0667 0.0587 0.047 0.0562 0.0308 0.0537 0.0433 0.0536 0.0589 0.0505 0.026 0.0629 0.0474 0.0576 0.0374 0.0258 0.027
Eisosomes 0.0006 0 0.0001 0.0001 0 0.0001 0.0002 0 0.0001 0 0 0 0.0003 0 0.0002 0.0003 0.0002 0
Endoplasmic Reticulum 0.0089 0.0007 0.0012 0.0009 0.0001 0.0024 0.001 0.0001 0.0003 0.0003 0 0.0003 0.0015 0.0004 0.0014 0.0038 0.0093 0.0002
Endosome 0.06 0.0363 0.031 0.0373 0.0083 0.0206 0.0144 0.0152 0.0221 0.0388 0.0165 0.0075 0.0137 0.0126 0.022 0.0396 0.0429 0.0043
Golgi 0.0505 0.0551 0.0647 0.041 0.0283 0.0409 0.0273 0.03 0.0615 0.0866 0.0542 0.0301 0.0271 0.0458 0.0491 0.0951 0.0585 0.0279
Lipid Particles 0.6384 0.7168 0.7257 0.7703 0.8188 0.8117 0.801 0.8558 0.7661 0.7244 0.7859 0.8922 0.6922 0.8571 0.7603 0.7408 0.7131 0.8628
Mitochondria 0.0363 0.0126 0.013 0.0471 0.0113 0.0428 0.0128 0.0064 0.0193 0.0089 0.0109 0.0064 0.0315 0.0023 0.0193 0.0143 0.0802 0.0049
None 0.0078 0.0002 0.0005 0.0001 0.0001 0.0004 0.0033 0 0.0002 0 0 0 0.0167 0 0.0009 0.0007 0.0108 0
Nuclear Periphery 0.0108 0.0156 0.0027 0.0009 0.0004 0.0048 0.0051 0.001 0.0035 0.0004 0.0001 0.0024 0.0032 0.0004 0.0022 0.0052 0.0199 0.0006
Nucleolus 0.0146 0.0266 0.0308 0.0003 0.001 0.003 0.0291 0.0133 0.0007 0.0007 0.0004 0.006 0.0104 0.0018 0.0012 0.0005 0.0007 0.0165
Nucleus 0.0112 0.0026 0.0022 0.0002 0.0002 0.001 0.002 0.0005 0.0001 0.0001 0 0.0002 0.0038 0.0001 0.0005 0.0005 0.0049 0.0006
Peroxisomes 0.025 0.0222 0.0388 0.0442 0.0359 0.0171 0.0255 0.0232 0.0539 0.0726 0.0781 0.022 0.023 0.018 0.0442 0.0229 0.0096 0.0174
Punctate Nuclear 0.0138 0.0242 0.0036 0.0011 0.0363 0.0034 0.0119 0.0008 0.0011 0.0007 0.0005 0.0014 0.0209 0.0073 0.0058 0.0031 0.0017 0.0109
Vacuole 0.0032 0.0071 0.0064 0.0023 0.0003 0.002 0.0013 0.0013 0.0013 0.0024 0.0005 0.0008 0.0013 0.001 0.0026 0.0013 0.0017 0.0004
Vacuole Periphery 0.0048 0.007 0.0044 0.0015 0.0009 0.0037 0.003 0.0031 0.0029 0.0036 0.0012 0.0035 0.0034 0.0034 0.0053 0.0026 0.0037 0.0017

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 96.178 102.0389 114.9781 122.822 92.4965 98.2374 135.1737 137.3333 142.364 134.7439
Translational Efficiency 1.3385 1.347 1.35 1.2721 1.1229 1.4371 1.2992 1.3859 1.163 1.0922

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘
Standard name
Human Ortholog
Description 3-keto sterol reductase; catalyzes the last of three steps required to remove two C-4 methyl groups from an intermediate in ergosterol biosynthesis; mutants are sterol auxotrophs; mutation is functionally complemented by human HSD17B7
Localization
Cell Percentages —
Cell Cycle Regulation No
Subcompartmental Group N/A

Erg27

Erg27


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Erg27-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available