Standard name
Human Ortholog
Description Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C); APC/C is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Cytoplasm 0.84 0.97 0.97 0.91 0.96 0.92 0.93 0.9 0.76 0.79 0.72 0.74 0.96 0.98 1.0 – – – 0.74 0.82 0.68 0.73 0.79 0.8
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Mitochondria 0.21 0.06 0 0.09 0 0.13 0.09 0.07 0.39 0.38 0.45 0.41 0 0 0 – – – 0.13 0.08 0.13 0 0.05 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0.06 0 0 0.05 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 1 0 0 1 0 0 – – – 0 1 4 0 3 3
Bud 0 1 0 0 0 0 2 0 4 4 6 9 0 1 0 – – – 0 0 7 3 11 3
Bud Neck 0 0 4 1 6 0 10 13 2 4 2 4 1 2 1 – – – 1 0 0 1 2 1
Bud Site 0 0 0 0 0 0 0 0 1 1 1 1 0 0 0 – – – – – – – – –
Cell Periphery 0 1 1 1 0 0 0 4 0 0 1 1 0 1 1 – – – 0 1 0 0 0 0
Cytoplasm 56 197 180 104 210 204 269 416 172 166 177 239 156 212 285 – – – 76 166 131 149 256 226
Endoplasmic Reticulum 1 1 1 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 3 1 1 2
Endosome 0 0 1 0 0 2 2 6 0 0 0 2 1 2 0 – – – 1 1 4 3 1 5
Golgi 0 0 0 0 1 0 1 1 0 0 0 0 0 0 0 – – – 0 0 0 2 0 1
Mitochondria 14 12 0 10 3 28 26 34 88 81 111 133 3 0 0 – – – 14 15 24 8 14 9
Nucleus 0 0 0 4 0 2 7 7 2 3 2 1 1 1 1 – – – 0 1 1 3 0 1
Nuclear Periphery 0 1 0 0 0 0 0 0 1 0 0 0 0 0 0 – – – 0 0 1 1 0 0
Nucleolus 0 0 1 2 1 1 1 1 0 0 0 1 0 0 0 – – – 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 – – – 0 0 1 0 0 0
SpindlePole 0 0 1 0 3 0 3 10 1 4 6 11 0 0 0 – – – 1 0 1 8 11 11
Vac/Vac Membrane 0 0 1 3 7 7 4 5 3 2 3 2 1 0 1 – – – 5 5 2 10 11 10
Unique Cell Count 67 203 186 114 218 222 288 461 226 211 247 325 163 217 286 104 203 193 203 323 283
Labelled Cell Count 71 213 190 125 231 244 325 497 274 266 309 405 164 219 289 104 203 193 203 323 283


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.6 2.8 5.2 4.8 5.5 4.1 4.4 4.6 3.5 3.5 3.5 3.5 4.6 4.7 4.6 – – – 5.3 5.4 5.3
Std Deviation (1e-4) 0.4 0.5 2.3 2.2 3.5 1.7 2.5 2.4 1.9 1.5 1.6 1.3 1.2 1.4 1.9 – – – 2.2 1.9 1.9
Intensity Change (Log2) – – – -0.13 0.06 -0.35 -0.25 -0.2 -0.6 -0.6 -0.58 -0.59 -0.18 -0.15 -0.18 – – – 0.01 0.05 0.01


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -0.6912 -0.6011 -0.7921 -1.302 -0.876 -0.786 2.3395 2.3093 2.4657 2.5519 1.2274 2.6336 1.5243 0.4048 0.375 0.5219 0.9263 0.4313
Actin 0.0172 0.0007 0.0074 0.0006 0.0236 0.0059 0.0376 0.0001 0.0085 0.0003 0.007 0.0018 0.0543 0.0001 0.006 0.0065 0.026 0.009
Bud 0.001 0.0009 0.0005 0.0003 0.0005 0.0002 0.0008 0 0.0001 0.0001 0.0002 0.0001 0.0015 0 0.0019 0.0017 0.0037 0.0003
Bud Neck 0.0111 0.0003 0.0016 0.0005 0.0096 0.0029 0.013 0.0002 0.0002 0.0002 0.001 0.0012 0.0036 0.0001 0.0007 0.0007 0.0006 0.0081
Bud Periphery 0.0019 0.0007 0.0006 0.0003 0.0007 0.0002 0.002 0.0001 0.0001 0.0001 0.0004 0.0002 0.003 0.0001 0.0011 0.0032 0.0307 0.0004
Bud Site 0.0072 0.0022 0.0025 0.0002 0.0016 0.0001 0.0019 0.0005 0.0003 0.0001 0.0003 0.0003 0.0074 0.0002 0.0163 0.0045 0.0021 0.0004
Cell Periphery 0.0003 0.0002 0.0001 0 0.0001 0 0.0003 0.0001 0 0 0 0 0.0007 0.0001 0.0001 0.0001 0.0004 0
Cytoplasm 0.1458 0.1323 0.1091 0.1772 0.2464 0.1824 0.0732 0.1333 0.0665 0.0668 0.1231 0.1317 0.0933 0.1304 0.1134 0.0718 0.1183 0.1734
Cytoplasmic Foci 0.0203 0.007 0.0108 0.0143 0.0237 0.0109 0.0095 0.0057 0.0037 0.0055 0.0088 0.0057 0.0542 0.0144 0.0062 0.0105 0.0093 0.0079
Eisosomes 0.0004 0.0002 0.0003 0.0001 0.0002 0.0001 0.0007 0.0001 0.002 0.0001 0.0001 0.0001 0.0004 0.0001 0.0001 0.0001 0.0009 0
Endoplasmic Reticulum 0.0071 0.0026 0.003 0.0015 0.0026 0.0016 0.0048 0.0012 0.0011 0.0017 0.0014 0.0013 0.0027 0.0011 0.0055 0.0039 0.0015 0.0018
Endosome 0.012 0.0026 0.0056 0.0031 0.0135 0.0039 0.0163 0.0022 0.0014 0.0009 0.0139 0.0021 0.0245 0.0009 0.0022 0.0089 0.017 0.0107
Golgi 0.0029 0.0001 0.0013 0.0001 0.0045 0.0017 0.0034 0.0003 0.0005 0.0001 0.0089 0.0006 0.0118 0.0001 0.0009 0.0033 0.0088 0.0038
Lipid Particles 0.0103 0.0011 0.0029 0.0004 0.0092 0.0015 0.0078 0.0156 0.0014 0.0001 0.0087 0.0031 0.0116 0.0002 0.0009 0.0054 0.0299 0.001
Mitochondria 0.0028 0.0004 0.0012 0.0006 0.0011 0.0012 0.0085 0.0024 0.0005 0.0002 0.0022 0.0007 0.0047 0.0002 0.0009 0.0257 0.0639 0.002
None 0.6196 0.774 0.7146 0.6573 0.5192 0.5969 0.755 0.7236 0.8589 0.8894 0.7291 0.797 0.6212 0.8354 0.8079 0.7859 0.654 0.7176
Nuclear Periphery 0.0211 0.0055 0.0079 0.0074 0.0139 0.0067 0.0107 0.0013 0.0066 0.0024 0.003 0.0033 0.0145 0.0013 0.0024 0.0103 0.0051 0.0054
Nucleolus 0.0031 0.0014 0.0022 0.0022 0.004 0.0025 0.0019 0.0017 0.0004 0.0004 0.0022 0.0008 0.0005 0.0002 0.0004 0.002 0.002 0.0005
Nucleus 0.0664 0.0322 0.0886 0.0747 0.0216 0.1351 0.0328 0.1012 0.0384 0.0051 0.0612 0.037 0.0683 0.0118 0.0245 0.0317 0.0059 0.0488
Peroxisomes 0.0083 0.0002 0.0019 0.0003 0.0065 0.0005 0.0052 0.0036 0.0031 0.0002 0.0021 0.0025 0.0133 0.0001 0.003 0.0119 0.0103 0.0021
Punctate Nuclear 0.0384 0.0337 0.0358 0.0577 0.0964 0.0447 0.0104 0.0046 0.006 0.026 0.0248 0.0098 0.0055 0.0028 0.0047 0.0089 0.0016 0.0049
Vacuole 0.0019 0.0014 0.0015 0.0011 0.0007 0.0008 0.0025 0.0018 0.0004 0.0005 0.001 0.0005 0.0022 0.0006 0.0006 0.0023 0.0054 0.0009
Vacuole Periphery 0.0009 0.0002 0.0005 0.0003 0.0005 0.0002 0.0017 0.0002 0.0001 0.0001 0.0004 0.0001 0.0008 0.0001 0.0001 0.0007 0.0027 0.0008

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 7.6049 4.9442 4.5861 6.7081 2.6891 4.1458 5.624 10.1222 8.3659 6.6896
Translational Efficiency 1.2695 2.6768 2.7277 2.0045 4.2102 1.9201 2.4355 1.6927 2.0195 1.4557

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C); APC/C is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition
Localization
Cell Percentages cytoplasm (30%), nucleus (4%), mixed (39%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Apc9

Apc9


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Apc9-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available