Standard name | |
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Human Ortholog | |
Description | DNA replication initiation factor; recruited to MCM pre-RC complexes at replication origins; promotes release of MCM from Mcm10p, recruits elongation machinery; binds tightly to ssDNA, which disrupts interaction with the MCM helicase and stalls it during replication stress; mutants in human homolog may cause velocardiofacial and DiGeorge syndromes |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 |
Bud | 0 | 0 | 0 | 0 | 1 | 1 | 4 | 4 | 4 | 1 | 2 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 4 | 11 | 6 | 2 | 8 | 9 |
Bud Neck | 1 | 0 | 0 | 1 | 0 | 0 | 6 | 5 | 1 | 4 | 0 | 2 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 2 |
Bud Site | 0 | 0 | 0 | 1 | 0 | 2 | 4 | 3 | 10 | 7 | 9 | 4 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 23 | 21 | 29 | 27 | 27 | 34 | 50 | 69 | 37 | 50 | 41 | 56 | 31 | 48 | 73 | 41 | 43 | 63 | 10 | 11 | 11 | 22 | 40 | 46 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 13 | 13 | 10 | 0 | 1 | 0 | 0 | 0 | 3 |
Endosome | 1 | 4 | 1 | 6 | 1 | 11 | 2 | 5 | 2 | 4 | 1 | 0 | 4 | 0 | 3 | 9 | 8 | 18 | 5 | 5 | 2 | 3 | 4 | 4 |
Golgi | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 2 | 0 | 0 | 0 | 0 |
Mitochondria | 61 | 12 | 2 | 17 | 11 | 72 | 87 | 81 | 141 | 122 | 221 | 140 | 0 | 1 | 0 | 10 | 12 | 11 | 4 | 5 | 3 | 4 | 8 | 9 |
Nucleus | 235 | 373 | 210 | 155 | 197 | 289 | 265 | 282 | 194 | 216 | 175 | 139 | 121 | 107 | 143 | 157 | 140 | 214 | 241 | 361 | 190 | 122 | 115 | 104 |
Nuclear Periphery | 0 | 0 | 1 | 1 | 0 | 2 | 0 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 1 | 1 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 2 |
Nucleolus | 5 | 5 | 0 | 0 | 1 | 3 | 1 | 0 | 2 | 6 | 3 | 3 | 0 | 1 | 4 | 1 | 3 | 0 | 11 | 22 | 10 | 1 | 3 | 1 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 3 | 1 | 9 | 5 | 6 | 4 | 7 | 12 | 4 | 9 | 2 | 9 | 0 | 3 | 7 | 1 | 2 | 5 | 13 | 14 | 15 | 3 | 9 | 18 |
Vac/Vac Membrane | 31 | 55 | 9 | 8 | 24 | 32 | 28 | 28 | 24 | 34 | 22 | 21 | 18 | 6 | 18 | 28 | 29 | 67 | 44 | 42 | 50 | 47 | 63 | 89 |
Unique Cell Count | 309 | 443 | 248 | 204 | 251 | 396 | 388 | 433 | 326 | 356 | 348 | 297 | 164 | 162 | 238 | 232 | 222 | 345 | 343 | 486 | 299 | 214 | 262 | 304 |
Labelled Cell Count | 360 | 472 | 261 | 221 | 268 | 452 | 454 | 493 | 420 | 454 | 478 | 377 | 175 | 167 | 251 | 261 | 252 | 389 | 343 | 486 | 299 | 214 | 262 | 304 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 4.2 | 5.5 | 5.7 | 5.5 | 5.7 | 4.7 | 4.7 | 5.1 | 4.1 | 4.4 | 3.7 | 4.2 | 6.2 | 6.0 | 6.1 | 7.1 | 7.4 | 6.9 | 5.6 | 5.4 | 5.6 |
Std Deviation (1e-4) | 0.6 | 0.9 | 1.3 | 1.8 | 1.2 | 1.1 | 1.4 | 1.2 | 1.0 | 1.0 | 0.9 | 1.3 | 1.2 | 1.1 | 1.2 | 1.6 | 2.0 | 1.6 | 1.5 | 0.9 | 1.1 |
Intensity Change (Log2) | – | – | – | -0.04 | 0.0 | -0.28 | -0.26 | -0.15 | -0.47 | -0.39 | -0.62 | -0.44 | 0.13 | 0.09 | 0.09 | 0.31 | 0.39 | 0.28 | -0.02 | -0.09 | -0.03 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0.5 | -0.3 | -1.3 | 0.4 | 1.5 | -0.1 | 0.8 | 0 | 2.3 | 2.0 | 4.5 | 5.1 | 1.9 | 2.3 | 2.2 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.8 | 3.9 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.3 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 4.0 | 2.5 | 0 | 0 | 6.9 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2.5 | 2.9 | 2.0 |
Nucleus | -2.3 | -1.8 | -3.5 | -4.6 | -5.5 | -6.5 | -6.4 | -8.7 | -9.2 | -2.7 | -4.4 | -6.1 | -4.4 | -5.4 | -6.0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | -0.7 | -0.8 | 0 | 0 | -0.6 | 0 | 0 | 0 | 0 | 0 | 0 | -0.4 | 0 | -2.0 | -1.7 |
Vacuole | 0.2 | 2.7 | 2.3 | 1.9 | 1.6 | 1.9 | 2.8 | 1.5 | 1.8 | 2.9 | 0 | 1.9 | 3.5 | 3.7 | 5.7 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 1.8606 | 0.787 | 1.0443 | 0.5749 | 0.7729 | 0.533 | 0.978 | 0.9715 | 1.1339 | 0.9287 | 1.1945 | 0.6456 | 1.5105 | 1.004 | 1.1946 | 1.5455 | 0.7355 | 0.5962 |
Actin | 0.049 | 0.0006 | 0.0078 | 0.0019 | 0.0032 | 0.0011 | 0.0359 | 0.0001 | 0.0119 | 0.0094 | 0.0056 | 0.0062 | 0.0794 | 0.0001 | 0.0288 | 0.049 | 0.0007 | 0.0038 |
Bud | 0.002 | 0.0003 | 0.0001 | 0.0006 | 0.0004 | 0.0002 | 0.0009 | 0.0001 | 0.0013 | 0.0012 | 0.0005 | 0.0002 | 0.0016 | 0.0001 | 0.0006 | 0.0012 | 0.0002 | 0.0002 |
Bud Neck | 0.0143 | 0.0004 | 0.0005 | 0.0011 | 0.0016 | 0.0025 | 0.0056 | 0.0004 | 0.0026 | 0.0185 | 0.0012 | 0.0025 | 0.009 | 0.0003 | 0.0025 | 0.0132 | 0.0045 | 0.002 |
Bud Periphery | 0.0035 | 0.0002 | 0.0002 | 0.0007 | 0.0007 | 0.0002 | 0.0015 | 0.0001 | 0.005 | 0.0032 | 0.0011 | 0.0003 | 0.0031 | 0 | 0.0009 | 0.0017 | 0.0003 | 0.0004 |
Bud Site | 0.0123 | 0.0017 | 0.0008 | 0.0011 | 0.0018 | 0.0003 | 0.007 | 0.0005 | 0.0028 | 0.0049 | 0.0042 | 0.0003 | 0.0098 | 0.0004 | 0.0069 | 0.0102 | 0.0029 | 0.0002 |
Cell Periphery | 0.0013 | 0.0001 | 0.0001 | 0.0001 | 0.0003 | 0.0001 | 0.0004 | 0 | 0.0014 | 0.0003 | 0.0003 | 0 | 0.0008 | 0 | 0.0003 | 0.0006 | 0.0001 | 0 |
Cytoplasm | 0.015 | 0.0303 | 0.0056 | 0.0058 | 0.0262 | 0.0089 | 0.0352 | 0.0126 | 0.0049 | 0.0103 | 0.0213 | 0.0016 | 0.0177 | 0.0221 | 0.0042 | 0.0119 | 0.0034 | 0.0023 |
Cytoplasmic Foci | 0.0296 | 0.0069 | 0.0075 | 0.0088 | 0.0044 | 0.0165 | 0.025 | 0.0005 | 0.0057 | 0.0039 | 0.0235 | 0.0004 | 0.0197 | 0.0028 | 0.0038 | 0.0044 | 0.004 | 0.0004 |
Eisosomes | 0.0008 | 0 | 0.0002 | 0.0001 | 0 | 0.0001 | 0.0005 | 0 | 0.0018 | 0.0001 | 0.0001 | 0.0001 | 0.0006 | 0 | 0.0003 | 0.0003 | 0 | 0 |
Endoplasmic Reticulum | 0.0073 | 0.0013 | 0.0022 | 0.0004 | 0.0118 | 0.001 | 0.0106 | 0.0004 | 0.0021 | 0.0023 | 0.0012 | 0.0005 | 0.0059 | 0.002 | 0.0036 | 0.0055 | 0.0005 | 0.0005 |
Endosome | 0.0807 | 0.0025 | 0.0051 | 0.0055 | 0.0356 | 0.0026 | 0.0419 | 0.0003 | 0.0123 | 0.0258 | 0.0229 | 0.0015 | 0.0272 | 0.0009 | 0.012 | 0.0144 | 0.0045 | 0.0026 |
Golgi | 0.0167 | 0.0015 | 0.0026 | 0.0022 | 0.0042 | 0.0023 | 0.0106 | 0 | 0.0054 | 0.0042 | 0.0082 | 0.0011 | 0.0114 | 0 | 0.0078 | 0.0071 | 0.0013 | 0.001 |
Lipid Particles | 0.043 | 0.0003 | 0.0079 | 0.0072 | 0.0076 | 0.0061 | 0.0318 | 0 | 0.0078 | 0.0048 | 0.0395 | 0.0008 | 0.0485 | 0.0002 | 0.0104 | 0.0066 | 0.0041 | 0.0039 |
Mitochondria | 0.0383 | 0.0068 | 0.0116 | 0.0064 | 0.0066 | 0.0109 | 0.0235 | 0.0004 | 0.0174 | 0.0047 | 0.0071 | 0.0086 | 0.0135 | 0.0003 | 0.0067 | 0.0067 | 0.0006 | 0.0008 |
None | 0.0121 | 0.0217 | 0.0184 | 0.007 | 0.0151 | 0.0121 | 0.016 | 0.039 | 0.0174 | 0.0184 | 0.0419 | 0.0181 | 0.0323 | 0.004 | 0.0023 | 0.0034 | 0.0008 | 0.001 |
Nuclear Periphery | 0.0559 | 0.0151 | 0.0089 | 0.0073 | 0.0538 | 0.0055 | 0.0364 | 0.0057 | 0.0206 | 0.0095 | 0.0139 | 0.0035 | 0.0371 | 0.006 | 0.0126 | 0.0201 | 0.0237 | 0.0125 |
Nucleolus | 0.0327 | 0.0134 | 0.0263 | 0.0088 | 0.0165 | 0.0191 | 0.0142 | 0.0123 | 0.0181 | 0.016 | 0.0216 | 0.0142 | 0.0111 | 0.0092 | 0.0284 | 0.0206 | 0.0252 | 0.0194 |
Nucleus | 0.492 | 0.8668 | 0.869 | 0.9009 | 0.7294 | 0.8448 | 0.6411 | 0.9024 | 0.8384 | 0.8189 | 0.6993 | 0.9107 | 0.6141 | 0.9208 | 0.8334 | 0.7782 | 0.8711 | 0.9162 |
Peroxisomes | 0.0143 | 0.0002 | 0.0036 | 0.0024 | 0.0011 | 0.0008 | 0.0179 | 0 | 0.0092 | 0.0049 | 0.0121 | 0.0022 | 0.0254 | 0 | 0.0043 | 0.0144 | 0.0019 | 0.0004 |
Punctate Nuclear | 0.0545 | 0.0277 | 0.0203 | 0.0298 | 0.0626 | 0.0638 | 0.0354 | 0.0247 | 0.0055 | 0.0216 | 0.0659 | 0.0269 | 0.0175 | 0.0298 | 0.0271 | 0.024 | 0.0489 | 0.0312 |
Vacuole | 0.0173 | 0.0015 | 0.0008 | 0.0013 | 0.0133 | 0.0006 | 0.0054 | 0.0004 | 0.0059 | 0.0133 | 0.0065 | 0.0002 | 0.0108 | 0.0008 | 0.002 | 0.0056 | 0.0011 | 0.0006 |
Vacuole Periphery | 0.0074 | 0.0005 | 0.0004 | 0.0007 | 0.0039 | 0.0006 | 0.0033 | 0.0001 | 0.0024 | 0.0039 | 0.0021 | 0.0001 | 0.0036 | 0.0001 | 0.0011 | 0.001 | 0.0003 | 0.0004 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 28.5315 | 8.0809 | 3.7477 | 12.5373 | 25.2719 | 23.716 | 7.5323 | 5.3423 | 8.8156 | 21.4765 |
Translational Efficiency | 0.7187 | 0.4324 | 0.6014 | 0.5641 | 0.4284 | 0.8765 | 0.6433 | 0.5434 | 0.4693 | 0.5628 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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