Standard name
Human Ortholog
Description DNA replication initiation factor; recruited to MCM pre-RC complexes at replication origins; promotes release of MCM from Mcm10p, recruits elongation machinery; binds tightly to ssDNA, which disrupts interaction with the MCM helicase and stalls it during replication stress; mutants in human homolog may cause velocardiofacial and DiGeorge syndromes

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.07 0.05 0.12 0.13 0.11 0.09 0.13 0.16 0.11 0.14 0.12 0.19 0.19 0.3 0.31 0.18 0.19 0.18 0 0 0 0.1 0.15 0.15
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.06 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.2 0 0 0.08 0 0.18 0.22 0.19 0.43 0.34 0.64 0.47 0 0 0 0 0.05 0 0 0 0 0 0 0
Nucleus 0.76 0.84 0.85 0.76 0.78 0.73 0.68 0.65 0.6 0.61 0.5 0.47 0.74 0.66 0.6 0.68 0.63 0.62 0.71 0.74 0.64 0.57 0.44 0.34
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0.06
Vac/Vac Membrane 0.1 0.12 0 0 0.1 0.08 0.07 0.06 0.07 0.1 0.06 0.07 0.11 0 0.08 0.12 0.13 0.19 0.13 0.09 0.17 0.22 0.24 0.3
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1
Bud 0 0 0 0 1 1 4 4 4 1 2 2 0 0 0 0 0 0 4 11 6 2 8 9
Bud Neck 1 0 0 1 0 0 6 5 1 4 0 2 0 0 1 0 0 0 0 0 2 0 0 2
Bud Site 0 0 0 1 0 2 4 3 10 7 9 4 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 23 21 29 27 27 34 50 69 37 50 41 56 31 48 73 41 43 63 10 11 11 22 40 46
Endoplasmic Reticulum 0 0 0 0 0 0 0 1 0 0 0 0 1 0 1 13 13 10 0 1 0 0 0 3
Endosome 1 4 1 6 1 11 2 5 2 4 1 0 4 0 3 9 8 18 5 5 2 3 4 4
Golgi 0 1 0 0 0 1 0 1 0 0 1 0 0 0 0 0 1 1 1 2 0 0 0 0
Mitochondria 61 12 2 17 11 72 87 81 141 122 221 140 0 1 0 10 12 11 4 5 3 4 8 9
Nucleus 235 373 210 155 197 289 265 282 194 216 175 139 121 107 143 157 140 214 241 361 190 122 115 104
Nuclear Periphery 0 0 1 1 0 2 0 1 1 1 1 1 0 0 1 1 1 0 1 0 0 0 0 2
Nucleolus 5 5 0 0 1 3 1 0 2 6 3 3 0 1 4 1 3 0 11 22 10 1 3 1
Peroxisomes 0 0 0 0 0 1 0 1 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0
SpindlePole 3 1 9 5 6 4 7 12 4 9 2 9 0 3 7 1 2 5 13 14 15 3 9 18
Vac/Vac Membrane 31 55 9 8 24 32 28 28 24 34 22 21 18 6 18 28 29 67 44 42 50 47 63 89
Unique Cell Count 309 443 248 204 251 396 388 433 326 356 348 297 164 162 238 232 222 345 343 486 299 214 262 304
Labelled Cell Count 360 472 261 221 268 452 454 493 420 454 478 377 175 167 251 261 252 389 343 486 299 214 262 304


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.2 5.5 5.7 5.5 5.7 4.7 4.7 5.1 4.1 4.4 3.7 4.2 6.2 6.0 6.1 7.1 7.4 6.9 5.6 5.4 5.6
Std Deviation (1e-4) 0.6 0.9 1.3 1.8 1.2 1.1 1.4 1.2 1.0 1.0 0.9 1.3 1.2 1.1 1.2 1.6 2.0 1.6 1.5 0.9 1.1
Intensity Change (Log2) -0.04 0.0 -0.28 -0.26 -0.15 -0.47 -0.39 -0.62 -0.44 0.13 0.09 0.09 0.31 0.39 0.28 -0.02 -0.09 -0.03


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.5 -0.3 -1.3 0.4 1.5 -0.1 0.8 0 2.3 2.0 4.5 5.1 1.9 2.3 2.2
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 3.8 3.9 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 3.3
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 4.0 2.5 0 0 6.9 0 0 0 0 0 0 0 2.5 2.9 2.0
Nucleus -2.3 -1.8 -3.5 -4.6 -5.5 -6.5 -6.4 -8.7 -9.2 -2.7 -4.4 -6.1 -4.4 -5.4 -6.0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole -0.7 -0.8 0 0 -0.6 0 0 0 0 0 0 -0.4 0 -2.0 -1.7
Vacuole 0.2 2.7 2.3 1.9 1.6 1.9 2.8 1.5 1.8 2.9 0 1.9 3.5 3.7 5.7

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.8606 0.787 1.0443 0.5749 0.7729 0.533 0.978 0.9715 1.1339 0.9287 1.1945 0.6456 1.5105 1.004 1.1946 1.5455 0.7355 0.5962
Actin 0.049 0.0006 0.0078 0.0019 0.0032 0.0011 0.0359 0.0001 0.0119 0.0094 0.0056 0.0062 0.0794 0.0001 0.0288 0.049 0.0007 0.0038
Bud 0.002 0.0003 0.0001 0.0006 0.0004 0.0002 0.0009 0.0001 0.0013 0.0012 0.0005 0.0002 0.0016 0.0001 0.0006 0.0012 0.0002 0.0002
Bud Neck 0.0143 0.0004 0.0005 0.0011 0.0016 0.0025 0.0056 0.0004 0.0026 0.0185 0.0012 0.0025 0.009 0.0003 0.0025 0.0132 0.0045 0.002
Bud Periphery 0.0035 0.0002 0.0002 0.0007 0.0007 0.0002 0.0015 0.0001 0.005 0.0032 0.0011 0.0003 0.0031 0 0.0009 0.0017 0.0003 0.0004
Bud Site 0.0123 0.0017 0.0008 0.0011 0.0018 0.0003 0.007 0.0005 0.0028 0.0049 0.0042 0.0003 0.0098 0.0004 0.0069 0.0102 0.0029 0.0002
Cell Periphery 0.0013 0.0001 0.0001 0.0001 0.0003 0.0001 0.0004 0 0.0014 0.0003 0.0003 0 0.0008 0 0.0003 0.0006 0.0001 0
Cytoplasm 0.015 0.0303 0.0056 0.0058 0.0262 0.0089 0.0352 0.0126 0.0049 0.0103 0.0213 0.0016 0.0177 0.0221 0.0042 0.0119 0.0034 0.0023
Cytoplasmic Foci 0.0296 0.0069 0.0075 0.0088 0.0044 0.0165 0.025 0.0005 0.0057 0.0039 0.0235 0.0004 0.0197 0.0028 0.0038 0.0044 0.004 0.0004
Eisosomes 0.0008 0 0.0002 0.0001 0 0.0001 0.0005 0 0.0018 0.0001 0.0001 0.0001 0.0006 0 0.0003 0.0003 0 0
Endoplasmic Reticulum 0.0073 0.0013 0.0022 0.0004 0.0118 0.001 0.0106 0.0004 0.0021 0.0023 0.0012 0.0005 0.0059 0.002 0.0036 0.0055 0.0005 0.0005
Endosome 0.0807 0.0025 0.0051 0.0055 0.0356 0.0026 0.0419 0.0003 0.0123 0.0258 0.0229 0.0015 0.0272 0.0009 0.012 0.0144 0.0045 0.0026
Golgi 0.0167 0.0015 0.0026 0.0022 0.0042 0.0023 0.0106 0 0.0054 0.0042 0.0082 0.0011 0.0114 0 0.0078 0.0071 0.0013 0.001
Lipid Particles 0.043 0.0003 0.0079 0.0072 0.0076 0.0061 0.0318 0 0.0078 0.0048 0.0395 0.0008 0.0485 0.0002 0.0104 0.0066 0.0041 0.0039
Mitochondria 0.0383 0.0068 0.0116 0.0064 0.0066 0.0109 0.0235 0.0004 0.0174 0.0047 0.0071 0.0086 0.0135 0.0003 0.0067 0.0067 0.0006 0.0008
None 0.0121 0.0217 0.0184 0.007 0.0151 0.0121 0.016 0.039 0.0174 0.0184 0.0419 0.0181 0.0323 0.004 0.0023 0.0034 0.0008 0.001
Nuclear Periphery 0.0559 0.0151 0.0089 0.0073 0.0538 0.0055 0.0364 0.0057 0.0206 0.0095 0.0139 0.0035 0.0371 0.006 0.0126 0.0201 0.0237 0.0125
Nucleolus 0.0327 0.0134 0.0263 0.0088 0.0165 0.0191 0.0142 0.0123 0.0181 0.016 0.0216 0.0142 0.0111 0.0092 0.0284 0.0206 0.0252 0.0194
Nucleus 0.492 0.8668 0.869 0.9009 0.7294 0.8448 0.6411 0.9024 0.8384 0.8189 0.6993 0.9107 0.6141 0.9208 0.8334 0.7782 0.8711 0.9162
Peroxisomes 0.0143 0.0002 0.0036 0.0024 0.0011 0.0008 0.0179 0 0.0092 0.0049 0.0121 0.0022 0.0254 0 0.0043 0.0144 0.0019 0.0004
Punctate Nuclear 0.0545 0.0277 0.0203 0.0298 0.0626 0.0638 0.0354 0.0247 0.0055 0.0216 0.0659 0.0269 0.0175 0.0298 0.0271 0.024 0.0489 0.0312
Vacuole 0.0173 0.0015 0.0008 0.0013 0.0133 0.0006 0.0054 0.0004 0.0059 0.0133 0.0065 0.0002 0.0108 0.0008 0.002 0.0056 0.0011 0.0006
Vacuole Periphery 0.0074 0.0005 0.0004 0.0007 0.0039 0.0006 0.0033 0.0001 0.0024 0.0039 0.0021 0.0001 0.0036 0.0001 0.0011 0.001 0.0003 0.0004

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 28.5315 8.0809 3.7477 12.5373 25.2719 23.716 7.5323 5.3423 8.8156 21.4765
Translational Efficiency 0.7187 0.4324 0.6014 0.5641 0.4284 0.8765 0.6433 0.5434 0.4693 0.5628

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency
Standard name
Human Ortholog
Description DNA replication initiation factor; recruited to MCM pre-RC complexes at replication origins; promotes release of MCM from Mcm10p, recruits elongation machinery; binds tightly to ssDNA, which disrupts interaction with the MCM helicase and stalls it during replication stress; mutants in human homolog may cause velocardiofacial and DiGeorge syndromes
Localization
Cell Percentages nucleus (95%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-3

Cdc45

Cdc45


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Cdc45-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available