Standard name
Human Ortholog
Description Subunit of the tRNA splicing endonuclease; tRNA splicing endonuclease (Sen complex) is composed of Sen2p, Sen15p, Sen34p, and Sen54p; Sen complex also cleaves the CBP1 mRNA at the mitochondrial surface; Sen2p contains the active site for tRNA 5' splice site cleavage and has similarity to Sen34p and to Archaeal tRNA splicing endonuclease;
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0.06 0.06 0.05 0 0.06 0.06 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.09 0.05 0 0 0 0 0 0 0
Cytoplasm 0.2 0.2 0.39 0.45 0.4 0.26 0.2 0.28 0.21 0.31 0.21 0.22 0.58 0.58 0.67 0.52 0.49 0.42 0 0 0 0 0 0
Endoplasmic Reticulum 0 0.1 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.08 0.08 0 0 0 0 0 0
Endosome 0.08 0 0 0 0.08 0 0 0 0 0 0 0 0.07 0.17 0.07 0.1 0.07 0.16 0.08 0.09 0.07 0.05 0.05 0.06
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.73 0.78 0.31 0.47 0.41 0.73 0.8 0.73 0.83 0.7 0.84 0.75 0.19 0.09 0.1 0.13 0.16 0.12 0.72 0.75 0.73 0.75 0.67 0.68
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0.12 0.09 0.09 0.1 0.1 0 0 0 0 0 0
SpindlePole 0 0 0.16 0.11 0.11 0.07 0.1 0.08 0.05 0.07 0 0.08 0 0 0 0.11 0.09 0.1 0 0 0 0 0 0
Vac/Vac Membrane 0.1 0.06 0.08 0.05 0.08 0 0 0 0 0 0 0 0.13 0.16 0.09 0.06 0.1 0.12 0.06 0 0 0.05 0.05 0.06
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 3 7 2 0 2 0 1 0 0 1 0 2 1 1 0 2 4 6 0 8 2 8 16
Bud 11 2 7 5 4 14 23 26 22 16 24 20 4 0 2 0 4 6 2 0 1 2 7 4
Bud Neck 1 0 9 0 4 4 2 5 3 5 1 11 4 2 0 0 2 0 5 0 2 2 8 12
Bud Site 3 0 0 0 0 1 9 9 2 3 4 4 0 0 0 0 0 0 – – – – – –
Cell Periphery 6 0 3 7 2 2 5 1 3 2 1 3 5 8 7 9 7 6 0 0 2 0 0 2
Cytoplasm 83 10 75 99 111 86 76 127 84 125 82 77 125 120 176 54 66 61 10 0 6 5 13 9
Endoplasmic Reticulum 4 5 1 0 1 2 1 0 2 0 1 2 0 0 3 5 11 11 6 0 6 1 5 5
Endosome 33 0 5 8 22 2 0 2 0 2 0 0 15 34 18 10 9 24 37 8 22 13 17 23
Golgi 0 2 0 2 0 0 0 0 0 0 0 0 1 1 3 1 4 0 8 3 5 3 3 7
Mitochondria 306 39 59 104 112 236 304 327 335 281 327 266 40 19 25 14 22 17 349 65 236 188 242 292
Nucleus 1 0 1 1 2 8 7 10 5 11 4 2 0 0 1 3 2 3 0 0 0 0 1 1
Nuclear Periphery 2 1 0 0 0 0 1 2 0 0 1 0 0 0 0 0 0 0 2 0 2 2 1 2
Nucleolus 2 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 2 0 0 0 1 2 1
Peroxisomes 9 0 5 11 11 3 3 1 0 2 2 3 7 24 24 9 13 14 10 3 8 2 10 7
SpindlePole 4 1 30 23 29 23 38 37 20 27 17 28 8 6 4 11 12 14 5 0 4 7 8 8
Vac/Vac Membrane 40 3 15 10 23 14 12 10 11 15 13 12 27 33 23 6 13 18 28 3 8 12 19 26
Unique Cell Count 418 50 190 219 275 325 381 450 405 400 391 354 214 206 261 104 136 146 485 88 325 251 360 429
Labelled Cell Count 505 66 217 272 321 397 481 558 487 489 478 429 238 248 287 122 167 180 485 88 325 251 360 429


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.8 4.7 5.0 4.7 4.8 3.7 3.5 4.1 3.5 3.6 3.3 3.6 5.2 5.7 5.5 5.4 5.8 5.6 5.0 5.4 5.6
Std Deviation (1e-4) 0.6 0.5 1.5 1.5 1.9 1.2 1.3 1.6 1.2 1.1 1.1 0.8 1.1 1.8 1.4 1.3 1.9 1.6 1.4 1.8 1.6
Intensity Change (Log2) – – – -0.09 -0.05 -0.45 -0.5 -0.29 -0.53 -0.48 -0.62 -0.5 0.06 0.18 0.13 0.12 0.22 0.16 -0.0 0.11 0.16


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – 0 0 0
Bud Neck – – – – – – – – – – – – 0 0 0
Bud Site – – – – – – – – – – – – 0 0 0
Cell Periphery – – – – – – – – – – – – 0 0 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – 0 0 0
Endosome – – – – – – – – – – – – 0 0 0
Golgi – – – – – – – – – – – – 0 0 0
Mitochondria – – – – – – – – – – – – 0 0 0
Nuclear Periphery – – – – – – – – – – – – 0 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – 0 0 0
Peroxisomes – – – – – – – – – – – – 0 0 0
SpindlePole – – – – – – – – – – – – 0 0 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – 0 0 0
Cytoplasm – – – – – – – – – – – – 0 0 0
Nucleus – – – – – – – – – – – – 0 0 0
Vacuole – – – – – – – – – – – – -0.7 0.5 1.4

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.3596 1.4812 1.3368 1.2502 1.391 1.3679 0.3323 0.0502 0.5241 0.7297 -0.1257 0.305 0.6544 0.2688 0.0797 0.5378 0.2353 0.1621
Actin 0.024 0.0156 0.0103 0.0021 0.0042 0.0139 0.0463 0.0061 0.028 0.0994 0.0078 0.0061 0.0442 0.0092 0.028 0.0047 0.006 0.0103
Bud 0.0022 0.0058 0.0053 0.0029 0.0021 0.0025 0.0019 0.0107 0.0093 0.0074 0.0026 0.002 0.0043 0.0086 0.0079 0.0074 0.003 0.0023
Bud Neck 0.007 0.0023 0.0043 0.001 0.0016 0.0048 0.0132 0.0031 0.0079 0.0131 0.0027 0.0132 0.0075 0.0018 0.0033 0.0209 0.0013 0.0069
Bud Periphery 0.0056 0.0125 0.0177 0.0089 0.0057 0.0084 0.0052 0.0153 0.0098 0.0224 0.0082 0.0078 0.008 0.0106 0.0149 0.0119 0.0162 0.0068
Bud Site 0.0176 0.0343 0.0213 0.0032 0.0042 0.0031 0.013 0.0871 0.0402 0.0165 0.0015 0.0034 0.016 0.0531 0.0244 0.0077 0.0017 0.0021
Cell Periphery 0.0041 0.0017 0.0043 0.002 0.0005 0.0024 0.0027 0.005 0.003 0.0053 0.0009 0.0028 0.0023 0.0024 0.0014 0.001 0.0021 0.001
Cytoplasm 0.0633 0.0905 0.0861 0.0436 0.0241 0.082 0.087 0.116 0.118 0.0779 0.1254 0.1109 0.0957 0.1399 0.089 0.0736 0.1294 0.1072
Cytoplasmic Foci 0.1219 0.1435 0.1076 0.0958 0.0899 0.1132 0.1211 0.1199 0.1233 0.077 0.144 0.1497 0.1158 0.1267 0.1174 0.1106 0.0352 0.1315
Eisosomes 0.0019 0.0007 0.0009 0.0008 0.0003 0.0008 0.0012 0.0008 0.0037 0.0152 0.0005 0.0005 0.0012 0.0005 0.0006 0.0003 0.0003 0.0005
Endoplasmic Reticulum 0.0065 0.0025 0.0042 0.0036 0.0015 0.0031 0.0099 0.0057 0.0063 0.0056 0.0143 0.0072 0.0125 0.0145 0.0072 0.0031 0.0014 0.0101
Endosome 0.1558 0.1229 0.1249 0.145 0.118 0.1141 0.1315 0.146 0.0929 0.1409 0.0772 0.1252 0.2244 0.1991 0.1598 0.2137 0.0951 0.2103
Golgi 0.0555 0.0465 0.0367 0.0308 0.0497 0.0472 0.0455 0.0472 0.0377 0.0206 0.0343 0.0332 0.0518 0.0508 0.05 0.0591 0.063 0.0474
Lipid Particles 0.0352 0.0112 0.0173 0.0119 0.0212 0.0148 0.0245 0.015 0.0122 0.0177 0.0221 0.0227 0.0277 0.0249 0.01 0.004 0.0089 0.0224
Mitochondria 0.2649 0.284 0.352 0.4259 0.4638 0.383 0.2296 0.1863 0.2111 0.2158 0.2039 0.2953 0.1915 0.1786 0.3044 0.3781 0.5486 0.2733
None 0.1411 0.1413 0.133 0.1505 0.0804 0.1352 0.2174 0.1637 0.2308 0.1214 0.2435 0.1218 0.1052 0.0989 0.1225 0.0552 0.036 0.1093
Nuclear Periphery 0.0075 0.0013 0.0024 0.0016 0.0199 0.0036 0.0049 0.0019 0.0051 0.0063 0.0024 0.0091 0.0183 0.0046 0.0049 0.0011 0.0132 0.0048
Nucleolus 0.0026 0.0019 0.0025 0.0018 0.0102 0.001 0.002 0.0031 0.0027 0.0077 0.0011 0.0031 0.0043 0.0018 0.001 0.0011 0.0008 0.0012
Nucleus 0.0063 0.0047 0.0028 0.0023 0.0039 0.0017 0.0034 0.004 0.0042 0.008 0.0037 0.007 0.0091 0.0038 0.0026 0.0021 0.0024 0.0081
Peroxisomes 0.0497 0.0599 0.043 0.0502 0.071 0.052 0.0199 0.0349 0.0317 0.0514 0.0894 0.0522 0.0234 0.0231 0.0245 0.0191 0.0041 0.0215
Punctate Nuclear 0.0056 0.0035 0.002 0.0018 0.0173 0.0034 0.0039 0.0027 0.0053 0.0551 0.0043 0.0045 0.0066 0.0029 0.0021 0.001 0.0232 0.0041
Vacuole 0.0168 0.0097 0.016 0.0101 0.0066 0.0063 0.0123 0.0203 0.0129 0.0108 0.0073 0.0167 0.021 0.0349 0.0172 0.0172 0.0046 0.0119
Vacuole Periphery 0.005 0.0037 0.0053 0.0041 0.0042 0.0035 0.0035 0.0051 0.0041 0.0044 0.0028 0.0055 0.0094 0.0095 0.0069 0.0069 0.0034 0.0069

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 13.6223 12.1775 10.3271 13.6808 10.0925 15.5597 14.5113 16.9172 12.8587 15.8813
Translational Efficiency 0.7321 0.6792 0.6906 0.4783 0.8518 0.6078 0.5347 0.4626 0.6424 0.5315

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✔
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Subunit of the tRNA splicing endonuclease; tRNA splicing endonuclease (Sen complex) is composed of Sen2p, Sen15p, Sen34p, and Sen54p; Sen complex also cleaves the CBP1 mRNA at the mitochondrial surface; Sen2p contains the active site for tRNA 5' splice site cleavage and has similarity to Sen34p and to Archaeal tRNA splicing endonuclease;
Localization
Cell Percentages mitochondrion (91%)
Cell Cycle Regulation No
Subcompartmental Group mito-1

Sen2

Sen2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Sen2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available