Standard name | |
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Human Ortholog | |
Description | RNA exonuclease; required for maturation of the RNA component of RNase MRP; functions redundantly with Rnh70p and Rex2p in processing of U5 snRNA and RNase P RNA; member of RNase D family of exonucleases |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 1 |
Bud | 0 | 0 | 0 | 2 | 1 | 4 | 6 | 5 | 4 | 10 | 3 | 18 | 0 | 0 | 1 | 0 | 0 | 1 | 1 | 2 | 0 | 5 | 9 | 12 |
Bud Neck | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 4 | 6 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 4 | 8 | 7 | 4 | 10 | 8 | 15 | 0 | 0 | 0 | 0 | 1 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 3 | 17 | 29 | 32 | 47 | 73 | 58 | 68 | 28 | 56 | 27 | 55 | 77 | 140 | 147 | 34 | 38 | 28 | 3 | 20 | 12 | 34 | 55 | 55 |
Endoplasmic Reticulum | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 3 | 3 | 19 | 11 | 7 | 0 | 0 | 1 | 0 | 1 | 0 |
Endosome | 0 | 1 | 17 | 9 | 16 | 3 | 2 | 5 | 0 | 0 | 0 | 1 | 9 | 13 | 8 | 19 | 14 | 11 | 0 | 2 | 8 | 3 | 5 | 14 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 0 | 0 | 1 | 1 | 0 | 0 | 1 |
Mitochondria | 33 | 33 | 23 | 52 | 46 | 151 | 272 | 199 | 209 | 312 | 210 | 331 | 9 | 2 | 3 | 34 | 18 | 15 | 4 | 5 | 15 | 7 | 16 | 27 |
Nucleus | 14 | 74 | 63 | 60 | 39 | 66 | 98 | 79 | 71 | 93 | 62 | 104 | 234 | 242 | 129 | 183 | 144 | 98 | 39 | 81 | 81 | 127 | 147 | 132 |
Nuclear Periphery | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 4 | 0 | 5 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 1 | 1 | 3 |
Nucleolus | 0 | 1 | 1 | 1 | 2 | 2 | 1 | 0 | 2 | 0 | 1 | 1 | 0 | 1 | 0 | 4 | 3 | 1 | 0 | 1 | 3 | 4 | 11 | 9 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 2 | 0 | 3 | 5 | 2 | 5 | 3 | 2 | 1 | 2 | 9 | 5 | 3 | 7 | 11 | 1 | 3 | 9 | 14 | 13 | 38 | 41 |
Vac/Vac Membrane | 4 | 22 | 19 | 15 | 47 | 30 | 26 | 23 | 20 | 12 | 18 | 30 | 12 | 34 | 43 | 48 | 48 | 42 | 25 | 26 | 49 | 48 | 102 | 105 |
Unique Cell Count | 43 | 132 | 136 | 147 | 176 | 250 | 352 | 301 | 261 | 369 | 242 | 397 | 339 | 420 | 320 | 287 | 243 | 176 | 80 | 156 | 197 | 255 | 411 | 430 |
Labelled Cell Count | 54 | 150 | 154 | 171 | 203 | 340 | 473 | 393 | 341 | 500 | 331 | 564 | 354 | 441 | 337 | 350 | 290 | 205 | 80 | 156 | 197 | 255 | 411 | 430 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 3.3 | 4.3 | 4.4 | 4.4 | 4.1 | 3.6 | 3.2 | 3.4 | 3.1 | 3.0 | 2.8 | 2.9 | 5.4 | 5.5 | 5.3 | 6.1 | 6.2 | 6.2 | 5.2 | 5.7 | 5.9 |
Std Deviation (1e-4) | 0.3 | 0.8 | 1.5 | 2.6 | 1.9 | 2.1 | 1.5 | 1.6 | 0.8 | 1.1 | 1.1 | 1.0 | 1.0 | 1.1 | 1.3 | 1.4 | 1.8 | 1.7 | 1.1 | 1.4 | 1.5 |
Intensity Change (Log2) | – | – | – | -0.01 | -0.09 | -0.27 | -0.43 | -0.36 | -0.5 | -0.57 | -0.63 | -0.62 | 0.29 | 0.32 | 0.27 | 0.48 | 0.51 | 0.51 | 0.25 | 0.38 | 0.43 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | 0.7 | 1.4 | 2.2 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 1.4113 | 0.564 | 0.6092 | 1.1349 | 0.9794 | 0.6734 | -0.2325 | -0.1535 | -0.2131 | -0.4881 | -0.1135 | -0.4647 | -1.2504 | -0.6245 | -1.0513 | -0.8759 | -1.263 | -0.9889 |
Actin | 0.0292 | 0 | 0.0016 | 0.0042 | 0.0024 | 0.0011 | 0.0252 | 0.0007 | 0.0064 | 0.0671 | 0.0212 | 0.0063 | 0.016 | 0.0002 | 0.0021 | 0.0145 | 0.0102 | 0.0072 |
Bud | 0.0003 | 0 | 0 | 0.0009 | 0.0001 | 0.0001 | 0.0012 | 0.0003 | 0.0003 | 0.0003 | 0.0024 | 0.0001 | 0.0003 | 0 | 0.0002 | 0.0006 | 0.0001 | 0.0002 |
Bud Neck | 0.012 | 0.0003 | 0.0009 | 0.0009 | 0.0003 | 0.0017 | 0.0033 | 0.0005 | 0.0019 | 0.0005 | 0.0047 | 0.0028 | 0.0088 | 0.0003 | 0.0025 | 0.0034 | 0.0007 | 0.0025 |
Bud Periphery | 0.0004 | 0 | 0.0001 | 0.0007 | 0.0001 | 0.0001 | 0.0022 | 0.0002 | 0.0003 | 0.0004 | 0.0033 | 0.0002 | 0.0006 | 0 | 0.0005 | 0.0017 | 0.0002 | 0.0005 |
Bud Site | 0.0023 | 0.0002 | 0.0003 | 0.0004 | 0.0002 | 0.0002 | 0.0087 | 0.0022 | 0.0036 | 0.0012 | 0.0097 | 0.0002 | 0.0029 | 0.0003 | 0.0005 | 0.0017 | 0.0003 | 0.0005 |
Cell Periphery | 0.0002 | 0 | 0.0002 | 0.0001 | 0.0001 | 0 | 0.0007 | 0 | 0.0001 | 0.0001 | 0.0004 | 0 | 0.0002 | 0 | 0.0001 | 0.0002 | 0 | 0 |
Cytoplasm | 0.0339 | 0.0096 | 0.0276 | 0.0465 | 0.0056 | 0.0106 | 0.0244 | 0.0209 | 0.0248 | 0.0128 | 0.0238 | 0.0068 | 0.0151 | 0.0087 | 0.0143 | 0.0283 | 0.0042 | 0.0089 |
Cytoplasmic Foci | 0.013 | 0.0001 | 0.0007 | 0.0021 | 0.0011 | 0.0011 | 0.037 | 0.0024 | 0.0124 | 0.0147 | 0.0209 | 0.0005 | 0.0055 | 0.0004 | 0.005 | 0.0007 | 0.0119 | 0.0021 |
Eisosomes | 0.0004 | 0 | 0.0001 | 0.0001 | 0.0002 | 0 | 0.0005 | 0 | 0.0001 | 0.0002 | 0.0001 | 0 | 0.0004 | 0 | 0.0001 | 0.0002 | 0 | 0.0001 |
Endoplasmic Reticulum | 0.0092 | 0.0004 | 0.0052 | 0.0022 | 0.0012 | 0.0049 | 0.0067 | 0.0012 | 0.0012 | 0.0002 | 0.0022 | 0.0008 | 0.0027 | 0.0008 | 0.0011 | 0.0016 | 0.001 | 0.0016 |
Endosome | 0.0193 | 0.0003 | 0.0019 | 0.0024 | 0.0096 | 0.0028 | 0.0315 | 0.0015 | 0.0089 | 0.0095 | 0.047 | 0.0018 | 0.0118 | 0.0007 | 0.0057 | 0.0063 | 0.0086 | 0.006 |
Golgi | 0.0041 | 0 | 0.0002 | 0.0002 | 0.0033 | 0.0003 | 0.0103 | 0.0002 | 0.0087 | 0.018 | 0.0272 | 0.0004 | 0.0029 | 0 | 0.0011 | 0.0047 | 0.0051 | 0.0025 |
Lipid Particles | 0.0055 | 0 | 0.0004 | 0.0002 | 0.0343 | 0.0003 | 0.0201 | 0.0002 | 0.0061 | 0.0221 | 0.0196 | 0.0003 | 0.0067 | 0.0001 | 0.0042 | 0.0018 | 0.018 | 0.0042 |
Mitochondria | 0.0021 | 0.0003 | 0.0006 | 0.0027 | 0.0303 | 0.0005 | 0.0297 | 0.0006 | 0.0098 | 0.018 | 0.0066 | 0.0004 | 0.0101 | 0.0003 | 0.0055 | 0.0017 | 0.0009 | 0.0014 |
None | 0.1012 | 0.0209 | 0.0585 | 0.1215 | 0.0095 | 0.0481 | 0.0177 | 0.0242 | 0.0321 | 0.0008 | 0.0033 | 0.0019 | 0.0243 | 0.0114 | 0.033 | 0.006 | 0.001 | 0.0029 |
Nuclear Periphery | 0.0268 | 0.0065 | 0.0342 | 0.0447 | 0.0482 | 0.0186 | 0.0352 | 0.021 | 0.0232 | 0.02 | 0.0116 | 0.0092 | 0.0333 | 0.0256 | 0.0248 | 0.0184 | 0.0521 | 0.0128 |
Nucleolus | 0.013 | 0.0085 | 0.0069 | 0.0048 | 0.0099 | 0.0198 | 0.0128 | 0.011 | 0.0083 | 0.0047 | 0.0177 | 0.0182 | 0.0095 | 0.0071 | 0.0089 | 0.0072 | 0.0081 | 0.014 |
Nucleus | 0.6806 | 0.9322 | 0.8264 | 0.7253 | 0.8255 | 0.8615 | 0.6888 | 0.8901 | 0.8156 | 0.7755 | 0.7381 | 0.9279 | 0.826 | 0.9092 | 0.8565 | 0.8664 | 0.8099 | 0.9015 |
Peroxisomes | 0.0044 | 0 | 0 | 0.0003 | 0.0024 | 0.0001 | 0.0251 | 0.0003 | 0.0065 | 0.0292 | 0.0078 | 0.0001 | 0.0016 | 0.0001 | 0.0014 | 0.0003 | 0.0211 | 0.0014 |
Punctate Nuclear | 0.0393 | 0.0197 | 0.0332 | 0.0376 | 0.0122 | 0.0271 | 0.012 | 0.0203 | 0.0268 | 0.0038 | 0.0259 | 0.0213 | 0.018 | 0.0343 | 0.0307 | 0.0323 | 0.0458 | 0.0283 |
Vacuole | 0.0016 | 0.0007 | 0.0007 | 0.0013 | 0.0012 | 0.0009 | 0.0054 | 0.0018 | 0.0019 | 0.0005 | 0.0046 | 0.0005 | 0.0018 | 0.0005 | 0.0011 | 0.0014 | 0.0003 | 0.0009 |
Vacuole Periphery | 0.0011 | 0.0001 | 0.0003 | 0.0011 | 0.0025 | 0.0003 | 0.0016 | 0.0004 | 0.0012 | 0.0005 | 0.0017 | 0.0002 | 0.0012 | 0.0002 | 0.0008 | 0.0007 | 0.0004 | 0.0005 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 18.163 | 17.8603 | 20.4821 | 15.094 | 13.5408 | 18.153 | 17.0325 | 14.9584 | 15.3271 | 18.6368 |
Translational Efficiency | 0.8247 | 0.7855 | 0.6309 | 0.6985 | 0.8395 | 0.7958 | 0.7148 | 1.0648 | 0.829 | 0.7658 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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