Standard name | |
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Human Ortholog | |
Description | Conserved protein involved in exocytic transport from the Golgi; mutation is synthetically lethal with apl2 vps1 double mutation; member of a protein superfamily with orthologs in diverse organisms; relocalizes from bud neck to cytoplasm upon DNA replication stress |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 52 | 56 | 57 | 26 | 35 | 24 | 37 | 36 | 23 | 23 | 12 | 19 | 35 | 12 | 14 | 40 | 31 | 28 | 70 | 51 | 37 | 53 | 70 | 63 |
Bud | 29 | 32 | 25 | 15 | 28 | 31 | 28 | 22 | 22 | 28 | 26 | 27 | 22 | 14 | 9 | 10 | 9 | 3 | 35 | 46 | 43 | 74 | 144 | 116 |
Bud Neck | 0 | 1 | 2 | 1 | 0 | 0 | 1 | 1 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 1 | 4 | 0 | 14 | 17 | 19 | 16 | 17 | 10 |
Bud Site | 9 | 7 | 10 | 7 | 8 | 10 | 14 | 1 | 7 | 9 | 5 | 4 | 5 | 0 | 0 | 4 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 8 | 6 | 8 | 3 | 3 | 4 | 6 | 3 | 12 | 10 | 3 | 8 | 12 | 21 | 12 | 6 | 3 | 9 | 3 | 1 | 1 | 0 | 0 | 0 |
Cytoplasm | 97 | 95 | 88 | 63 | 76 | 133 | 172 | 213 | 137 | 116 | 113 | 125 | 177 | 260 | 304 | 44 | 31 | 49 | 55 | 69 | 51 | 5 | 23 | 32 |
Endoplasmic Reticulum | 11 | 18 | 9 | 7 | 2 | 4 | 17 | 20 | 7 | 0 | 5 | 4 | 5 | 14 | 30 | 4 | 5 | 5 | 23 | 11 | 13 | 9 | 22 | 23 |
Endosome | 15 | 14 | 24 | 19 | 15 | 37 | 16 | 20 | 5 | 6 | 5 | 7 | 15 | 6 | 7 | 28 | 11 | 14 | 22 | 19 | 24 | 8 | 18 | 13 |
Golgi | 6 | 9 | 2 | 5 | 6 | 1 | 3 | 0 | 0 | 0 | 0 | 0 | 6 | 4 | 4 | 39 | 28 | 33 | 5 | 8 | 8 | 1 | 8 | 4 |
Mitochondria | 28 | 10 | 5 | 19 | 7 | 36 | 34 | 20 | 68 | 99 | 89 | 59 | 8 | 2 | 5 | 11 | 10 | 6 | 11 | 13 | 24 | 11 | 10 | 9 |
Nucleus | 1 | 2 | 0 | 1 | 0 | 0 | 3 | 5 | 1 | 3 | 2 | 2 | 2 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 1 | 0 | 0 | 0 |
Nuclear Periphery | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 1 |
Nucleolus | 4 | 2 | 4 | 1 | 1 | 3 | 1 | 5 | 1 | 5 | 2 | 2 | 1 | 0 | 0 | 5 | 0 | 0 | 1 | 0 | 1 | 0 | 1 | 1 |
Peroxisomes | 9 | 6 | 11 | 5 | 4 | 15 | 0 | 0 | 0 | 2 | 1 | 2 | 11 | 3 | 0 | 3 | 2 | 0 | 1 | 1 | 2 | 5 | 5 | 0 |
SpindlePole | 2 | 5 | 34 | 6 | 18 | 14 | 8 | 16 | 3 | 8 | 7 | 11 | 1 | 3 | 2 | 28 | 12 | 21 | 3 | 9 | 16 | 7 | 10 | 9 |
Vac/Vac Membrane | 7 | 5 | 14 | 5 | 5 | 13 | 5 | 18 | 5 | 6 | 4 | 8 | 3 | 8 | 3 | 22 | 7 | 15 | 13 | 24 | 13 | 3 | 7 | 4 |
Unique Cell Count | 208 | 202 | 216 | 140 | 155 | 264 | 272 | 326 | 228 | 244 | 211 | 226 | 253 | 314 | 360 | 176 | 108 | 141 | 265 | 280 | 265 | 202 | 348 | 298 |
Labelled Cell Count | 279 | 268 | 293 | 184 | 208 | 325 | 345 | 381 | 291 | 320 | 274 | 278 | 303 | 347 | 390 | 245 | 154 | 184 | 265 | 280 | 265 | 202 | 348 | 298 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 10.0 | 9.8 | 9.2 | 8.0 | 8.6 | 7.4 | 7.2 | 7.2 | 6.4 | 5.9 | 5.9 | 6.2 | 10.3 | 10.4 | 10.2 | 12.0 | 12.2 | 11.7 | 9.5 | 9.1 | 9.8 |
Std Deviation (1e-4) | 1.5 | 1.8 | 1.1 | 1.1 | 1.2 | 1.2 | 1.3 | 1.1 | 1.0 | 0.9 | 1.1 | 1.1 | 1.3 | 1.4 | 1.6 | 2.2 | 2.1 | 2.1 | 1.8 | 1.6 | 1.9 |
Intensity Change (Log2) | – | – | – | -0.19 | -0.09 | -0.31 | -0.36 | -0.34 | -0.52 | -0.63 | -0.64 | -0.56 | 0.16 | 0.19 | 0.15 | 0.39 | 0.41 | 0.35 | 0.05 | -0.01 | 0.09 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | -1.7 | -0.8 | -5.0 | -3.6 | -4.6 | -4.5 | -4.8 | -5.8 | -5.0 | -3.4 | -7.6 | -8.0 | -0.8 | 0.4 | -1.4 |
Bud | -0.3 | 1.8 | 0.1 | -0.5 | -2.0 | -0.7 | 0 | 0.2 | 0.1 | -1.0 | -3.1 | -4.5 | -2.0 | -0.9 | -3.2 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0.2 | 0.2 | -0.5 | 0.3 | 0 | -0.9 | -0.5 | -1.3 | -1.7 | -1.6 | 0 | 0 | -1.3 | 0 | 0 |
Cell Periphery | 0 | 0 | -1.5 | -1.0 | 0 | 0.8 | 0.2 | -1.5 | -0.1 | 0.6 | 1.5 | -0.2 | -0.2 | 0 | 1.2 |
Cytoplasm | 0.8 | 1.6 | 2.1 | 4.9 | 5.6 | 4.1 | 1.5 | 2.7 | 3.1 | 6.4 | 10.0 | 10.9 | -3.3 | -2.1 | -1.1 |
Endoplasmic Reticulum | 0.4 | 0 | -1.8 | 1.0 | 1.0 | -0.6 | 0 | -1.0 | -1.5 | -1.4 | 0.2 | 1.9 | -1.0 | 0 | -0.3 |
Endosome | 0.7 | -0.4 | 1.0 | -2.1 | -2.1 | -3.8 | -3.8 | -3.6 | -3.3 | -2.0 | -4.5 | -4.7 | 1.4 | -0.3 | -0.4 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 6.8 | 7.3 | 7.0 |
Mitochondria | 4.1 | 1.2 | 4.4 | 4.1 | 2.1 | 7.8 | 9.8 | 9.9 | 7.1 | 0.6 | 0 | 0 | 2.0 | 2.8 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | -0.7 | -1.2 | 0.3 | 0 | 0 | -3.5 | -2.8 | -2.9 | -2.6 | -0.4 | -2.9 | 0 | -1.8 | 0 | 0 |
SpindlePole | -3.3 | -1.1 | -3.8 | -5.0 | -4.3 | -5.5 | -4.6 | -4.4 | -3.8 | -6.3 | -6.6 | -7.3 | 0 | -1.1 | -0.2 |
Vacuole | -1.2 | -1.4 | -0.7 | -2.6 | -0.5 | -2.2 | -2.1 | -2.4 | -1.4 | -3.1 | -2.2 | -3.9 | 2.1 | 0 | 1.4 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 4.4528 | 5.4043 | 4.9934 | 4.676 | 5.1531 | 5.4351 | 5.9347 | 6.9547 | 6.2885 | 6.9706 | 6.3219 | 7.0962 | 4.926 | 5.8515 | 5.1597 | 5.1652 | 4.8558 | 5.6117 |
Actin | 0.1453 | 0.1036 | 0.1783 | 0.1793 | 0.3182 | 0.1035 | 0.2456 | 0.1626 | 0.2582 | 0.2871 | 0.3735 | 0.2021 | 0.1786 | 0.0792 | 0.196 | 0.2132 | 0.2734 | 0.141 |
Bud | 0.046 | 0.0408 | 0.1917 | 0.0395 | 0.0315 | 0.0083 | 0.0343 | 0.0344 | 0.1495 | 0.0633 | 0.0324 | 0.0083 | 0.051 | 0.0311 | 0.1744 | 0.0537 | 0.0522 | 0.0064 |
Bud Neck | 0.0408 | 0.0057 | 0.0063 | 0.0064 | 0.0088 | 0.4577 | 0.0293 | 0.004 | 0.0078 | 0.0058 | 0.0547 | 0.4408 | 0.0414 | 0.0072 | 0.0071 | 0.0739 | 0.0044 | 0.4641 |
Bud Periphery | 0.0426 | 0.027 | 0.1758 | 0.1214 | 0.0386 | 0.0135 | 0.0293 | 0.0203 | 0.1544 | 0.1364 | 0.0449 | 0.0084 | 0.0517 | 0.0158 | 0.1885 | 0.0421 | 0.0936 | 0.0082 |
Bud Site | 0.1053 | 0.3005 | 0.0844 | 0.029 | 0.0386 | 0.0097 | 0.1626 | 0.3268 | 0.0681 | 0.0131 | 0.009 | 0.0129 | 0.166 | 0.3789 | 0.0989 | 0.0315 | 0.0118 | 0.0042 |
Cell Periphery | 0.0027 | 0.0023 | 0.0016 | 0.0022 | 0.0012 | 0.0015 | 0.0021 | 0.0014 | 0.0016 | 0.0022 | 0.0037 | 0.0013 | 0.0052 | 0.0021 | 0.0028 | 0.002 | 0.0015 | 0.001 |
Cytoplasm | 0.1614 | 0.3213 | 0.1439 | 0.3263 | 0.1648 | 0.1268 | 0.1489 | 0.227 | 0.1485 | 0.2089 | 0.1988 | 0.0986 | 0.1574 | 0.277 | 0.1768 | 0.3566 | 0.218 | 0.1109 |
Cytoplasmic Foci | 0.1503 | 0.0677 | 0.0567 | 0.0885 | 0.1111 | 0.0968 | 0.1175 | 0.092 | 0.0443 | 0.0588 | 0.0569 | 0.0813 | 0.118 | 0.1048 | 0.0327 | 0.0492 | 0.0505 | 0.1098 |
Eisosomes | 0.0007 | 0.0003 | 0.0004 | 0.0002 | 0.0004 | 0.0002 | 0.0005 | 0.0002 | 0.0003 | 0.0002 | 0.0002 | 0.0025 | 0.0014 | 0.0001 | 0.0004 | 0.0001 | 0.0003 | 0.0004 |
Endoplasmic Reticulum | 0.012 | 0.0074 | 0.0025 | 0.0066 | 0.0068 | 0.0099 | 0.0164 | 0.0028 | 0.0035 | 0.003 | 0.0036 | 0.0044 | 0.0257 | 0.0049 | 0.0038 | 0.0121 | 0.0136 | 0.0038 |
Endosome | 0.0888 | 0.0613 | 0.0637 | 0.099 | 0.1373 | 0.0575 | 0.0719 | 0.0573 | 0.0548 | 0.1023 | 0.0662 | 0.0313 | 0.0609 | 0.0392 | 0.0387 | 0.086 | 0.0746 | 0.0428 |
Golgi | 0.0695 | 0.0353 | 0.0516 | 0.0623 | 0.069 | 0.0706 | 0.082 | 0.0447 | 0.0565 | 0.0962 | 0.1001 | 0.0761 | 0.055 | 0.0324 | 0.035 | 0.0238 | 0.09 | 0.0579 |
Lipid Particles | 0.0148 | 0.0011 | 0.0041 | 0.0014 | 0.0057 | 0.0047 | 0.0092 | 0.0008 | 0.0069 | 0.0024 | 0.003 | 0.0028 | 0.0117 | 0.0019 | 0.0029 | 0.0223 | 0.005 | 0.0027 |
Mitochondria | 0.0359 | 0.0037 | 0.0151 | 0.0091 | 0.0113 | 0.0182 | 0.0192 | 0.0059 | 0.0277 | 0.0074 | 0.011 | 0.0067 | 0.0098 | 0.0034 | 0.0131 | 0.005 | 0.0587 | 0.0081 |
None | 0.0072 | 0.0038 | 0.0034 | 0.0015 | 0.0014 | 0.0007 | 0.0025 | 0.0012 | 0.0009 | 0.001 | 0.0006 | 0.0004 | 0.0083 | 0.0018 | 0.0093 | 0.0018 | 0.0035 | 0.0068 |
Nuclear Periphery | 0.0187 | 0.0007 | 0.0012 | 0.0009 | 0.0025 | 0.0007 | 0.0036 | 0.0007 | 0.0012 | 0.0005 | 0.0006 | 0.0003 | 0.0128 | 0.0005 | 0.0012 | 0.0011 | 0.0193 | 0.0023 |
Nucleolus | 0.0023 | 0.0003 | 0.0005 | 0.0003 | 0.0001 | 0.0002 | 0.0005 | 0.0003 | 0.0002 | 0.0001 | 0.0002 | 0.0002 | 0.0016 | 0.0004 | 0.0007 | 0.0006 | 0.0006 | 0.0007 |
Nucleus | 0.0055 | 0.0011 | 0.0016 | 0.0011 | 0.0007 | 0.0006 | 0.0013 | 0.0008 | 0.0007 | 0.0006 | 0.0006 | 0.0003 | 0.0085 | 0.0007 | 0.0016 | 0.0013 | 0.0067 | 0.0013 |
Peroxisomes | 0.0249 | 0.0072 | 0.0055 | 0.0037 | 0.0421 | 0.012 | 0.0152 | 0.0093 | 0.0083 | 0.0036 | 0.0324 | 0.0175 | 0.0223 | 0.0136 | 0.0056 | 0.0105 | 0.0074 | 0.0188 |
Punctate Nuclear | 0.0144 | 0.0013 | 0.002 | 0.0012 | 0.0022 | 0.0012 | 0.0028 | 0.0007 | 0.0008 | 0.0003 | 0.0006 | 0.0014 | 0.0039 | 0.0014 | 0.0008 | 0.0011 | 0.0072 | 0.0061 |
Vacuole | 0.0078 | 0.0064 | 0.0075 | 0.0161 | 0.0048 | 0.0036 | 0.0039 | 0.0057 | 0.004 | 0.0051 | 0.0048 | 0.002 | 0.0057 | 0.0031 | 0.0079 | 0.0103 | 0.005 | 0.0017 |
Vacuole Periphery | 0.0033 | 0.0011 | 0.002 | 0.0041 | 0.0026 | 0.0018 | 0.0015 | 0.0012 | 0.0017 | 0.0016 | 0.002 | 0.0006 | 0.003 | 0.0006 | 0.0018 | 0.0018 | 0.0026 | 0.0007 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 20.9142 | 30.0855 | 23.2816 | 26.327 | 22.7527 | 13.9351 | 24.9874 | 28.3036 | 29.0126 | 26.9924 |
Translational Efficiency | 0.9249 | 0.7129 | 0.8274 | 0.7362 | 0.7105 | 1.1145 | 0.8311 | 0.5474 | 0.5709 | 0.606 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✔ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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