Standard name | |
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Human Ortholog | |
Description | Component of commitment complex; which defines first step in splicing pathway; essential protein that interacts with Mud2p and Prp40p, forming a bridge between the intron ends; also involved in nuclear retention of pre-mRNA; relocalizes to the cytosol in response to hypoxia |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 1 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 1 | 1 | 2 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 2 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 6 | 3 | 10 | 5 | 10 | 1 | 0 | 1 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 7 | 11 | 6 | 4 | 7 | 16 | 11 | 13 | 6 | 12 | 5 | 0 | 25 | 41 | 39 | 32 | 18 | 11 | 1 | 2 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 13 | 7 | 4 | 0 | 0 | 0 | 0 | 1 | 0 |
Endosome | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 8 | 4 | 7 | 2 | 1 | 2 | 0 | 0 | 1 | 1 | 1 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 0 | 1 | 1 | 0 | 1 | 0 |
Mitochondria | 1 | 2 | 1 | 2 | 9 | 23 | 40 | 54 | 34 | 71 | 45 | 49 | 0 | 0 | 0 | 8 | 4 | 5 | 0 | 2 | 0 | 0 | 3 | 2 |
Nucleus | 170 | 196 | 54 | 63 | 106 | 125 | 126 | 144 | 103 | 95 | 114 | 128 | 182 | 246 | 171 | 597 | 410 | 379 | 163 | 198 | 68 | 109 | 124 | 119 |
Nuclear Periphery | 2 | 0 | 0 | 3 | 0 | 2 | 5 | 1 | 1 | 1 | 1 | 2 | 0 | 1 | 0 | 6 | 3 | 1 | 0 | 0 | 0 | 0 | 1 | 0 |
Nucleolus | 15 | 13 | 5 | 13 | 12 | 28 | 45 | 47 | 51 | 34 | 55 | 55 | 12 | 27 | 21 | 17 | 9 | 16 | 5 | 3 | 0 | 20 | 35 | 29 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 6 | 3 | 2 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 2 | 3 | 1 | 4 | 2 | 0 | 1 | 0 | 0 | 1 | 0 | 2 |
Vac/Vac Membrane | 4 | 6 | 0 | 1 | 2 | 3 | 2 | 4 | 3 | 4 | 1 | 3 | 5 | 1 | 5 | 18 | 23 | 14 | 4 | 5 | 1 | 7 | 22 | 26 |
Unique Cell Count | 183 | 215 | 60 | 71 | 123 | 158 | 174 | 198 | 145 | 146 | 161 | 171 | 216 | 309 | 228 | 666 | 459 | 416 | 183 | 219 | 74 | 143 | 196 | 191 |
Labelled Cell Count | 201 | 229 | 66 | 86 | 138 | 198 | 230 | 272 | 202 | 229 | 229 | 250 | 229 | 333 | 245 | 705 | 481 | 432 | 183 | 219 | 74 | 143 | 196 | 191 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 8.5 | 9.5 | 7.9 | 7.1 | 7.1 | 6.3 | 5.5 | 5.5 | 6.1 | 4.6 | 5.3 | 5.1 | 10.1 | 9.0 | 8.9 | 9.5 | 10.9 | 10.0 | 8.0 | 8.6 | 9.3 |
Std Deviation (1e-4) | 1.5 | 1.8 | 2.2 | 1.9 | 2.4 | 1.6 | 1.9 | 1.6 | 3.3 | 2.0 | 1.4 | 1.2 | 4.1 | 3.0 | 3.1 | 2.9 | 3.1 | 2.9 | 1.7 | 1.9 | 1.9 |
Intensity Change (Log2) | – | – | – | -0.15 | -0.15 | -0.34 | -0.54 | -0.53 | -0.38 | -0.78 | -0.58 | -0.63 | 0.35 | 0.19 | 0.17 | 0.27 | 0.47 | 0.34 | 0.02 | 0.12 | 0.24 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0 | 0 | 0 | 0 | 0 | 0 | -0.4 | 0 | 0 | 0.3 | 0.7 | 1.3 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 2.7 | 3.7 | 4.2 | 3.8 | 0 | 4.3 | 4.4 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | -0.2 | -0.7 | -1.9 | -2.8 | -2.8 | -2.9 | -3.6 | -3.0 | -2.5 | -1.1 | -1.9 | -2.5 | -0.1 | -0.2 | 0.3 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 1.7 | 0.3 | 1.7 | 2.9 | 2.6 | 3.9 | 0 | 3.8 | 3.6 | 0 | 0.1 | 0.2 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Actin | 0.035 | 0 | 0.0022 | 0 | 0.049 | 0.0056 | 0.0157 | 0.0156 | 0.0288 | 0.0003 | 0.0221 | 0.0014 | 0.0017 | 0 | 0.0417 | 0 | 0.0004 | 0 |
Bud | 0.0011 | 0 | 0.0001 | 0 | 0.0007 | 0.0003 | 0.0004 | 0 | 0.0003 | 0.0003 | 0.0007 | 0.0001 | 0.0003 | 0 | 0.0008 | 0.0001 | 0.0001 | 0.0001 |
Bud Neck | 0.0017 | 0.0001 | 0.0002 | 0.0001 | 0.0023 | 0.0007 | 0.0011 | 0 | 0.0009 | 0.0009 | 0.0014 | 0.0007 | 0.0004 | 0 | 0.0006 | 0.0002 | 0.0003 | 0.0019 |
Bud Periphery | 0.0018 | 0 | 0.0002 | 0 | 0.002 | 0.0004 | 0.0008 | 0 | 0.0009 | 0.0008 | 0.001 | 0.0001 | 0.0004 | 0 | 0.0009 | 0.0001 | 0.0002 | 0.0002 |
Bud Site | 0.0079 | 0 | 0.0004 | 0 | 0.003 | 0.0072 | 0.0012 | 0.0001 | 0.0063 | 0.0005 | 0.0024 | 0.0002 | 0.0002 | 0 | 0.0039 | 0 | 0 | 0.0001 |
Cell Periphery | 0.0005 | 0 | 0.0001 | 0 | 0.0006 | 0.0001 | 0.0001 | 0 | 0.0055 | 0.0001 | 0.0002 | 0 | 0.0001 | 0 | 0.0003 | 0 | 0 | 0 |
Cytoplasm | 0.0089 | 0 | 0.004 | 0 | 0.0047 | 0.0002 | 0.0046 | 0 | 0.0021 | 0.0008 | 0.0164 | 0.0019 | 0.0119 | 0 | 0.0037 | 0.0001 | 0.0001 | 0 |
Cytoplasmic Foci | 0.0192 | 0 | 0.0029 | 0 | 0.0081 | 0.003 | 0.0068 | 0 | 0.0035 | 0.0002 | 0.0169 | 0.0007 | 0.0137 | 0 | 0.0319 | 0 | 0 | 0 |
Eisosomes | 0.001 | 0 | 0 | 0 | 0.0006 | 0.0001 | 0.0001 | 0.0001 | 0.0003 | 0 | 0.0002 | 0 | 0 | 0 | 0.0004 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.003 | 0 | 0.002 | 0 | 0.0025 | 0.0002 | 0.0038 | 0 | 0.0024 | 0.0004 | 0.0016 | 0.0029 | 0.0039 | 0 | 0.0159 | 0 | 0.0001 | 0 |
Endosome | 0.0127 | 0 | 0.0114 | 0 | 0.0146 | 0.0025 | 0.0123 | 0.0001 | 0.0085 | 0.0014 | 0.0223 | 0.0038 | 0.0059 | 0 | 0.1645 | 0 | 0.0001 | 0 |
Golgi | 0.0071 | 0 | 0.002 | 0 | 0.0135 | 0.0011 | 0.0039 | 0.0004 | 0.0056 | 0.0001 | 0.0322 | 0.0007 | 0.0007 | 0 | 0.0841 | 0 | 0 | 0 |
Lipid Particles | 0.0221 | 0 | 0.0054 | 0 | 0.0288 | 0.0045 | 0.0053 | 0 | 0.0099 | 0.0003 | 0.0054 | 0.0024 | 0.0062 | 0 | 0.0959 | 0 | 0 | 0 |
Mitochondria | 0.0174 | 0.0001 | 0.0022 | 0.0001 | 0.0361 | 0.0008 | 0.0031 | 0.0003 | 0.0083 | 0.0012 | 0.0204 | 0.0017 | 0.0007 | 0.0001 | 0.0238 | 0.0002 | 0.0026 | 0.0003 |
None | 0.0058 | 0 | 0.0006 | 0 | 0.0024 | 0.0003 | 0.0016 | 0 | 0.0005 | 0.0004 | 0.0379 | 0.0005 | 0.0052 | 0 | 0.0018 | 0 | 0.0001 | 0 |
Nuclear Periphery | 0.0092 | 0.0013 | 0.0077 | 0.0002 | 0.0079 | 0.0049 | 0.0216 | 0.0004 | 0.0028 | 0.0068 | 0.0332 | 0.0022 | 0.3297 | 0.0002 | 0.0113 | 0.001 | 0.2171 | 0.0002 |
Nucleolus | 0.0193 | 0.0145 | 0.0134 | 0.0336 | 0.0785 | 0.0434 | 0.0222 | 0.0062 | 0.005 | 0.0197 | 0.0549 | 0.0335 | 0.0381 | 0.0262 | 0.029 | 0.0483 | 0.0803 | 0.034 |
Nucleus | 0.7979 | 0.982 | 0.9333 | 0.9655 | 0.7218 | 0.9177 | 0.8804 | 0.9764 | 0.8985 | 0.9592 | 0.714 | 0.945 | 0.1742 | 0.9731 | 0.4675 | 0.9475 | 0.6932 | 0.9629 |
Peroxisomes | 0.0172 | 0 | 0.0052 | 0 | 0.0089 | 0.0032 | 0.0039 | 0.0001 | 0.0057 | 0.0003 | 0.0062 | 0.0004 | 0.0004 | 0 | 0.0069 | 0 | 0 | 0 |
Punctate Nuclear | 0.006 | 0.002 | 0.0022 | 0.0004 | 0.0063 | 0.0034 | 0.0066 | 0.0002 | 0.0007 | 0.0044 | 0.005 | 0.0011 | 0.3983 | 0.0003 | 0.0084 | 0.0024 | 0.0008 | 0.0001 |
Vacuole | 0.0031 | 0 | 0.0018 | 0 | 0.0051 | 0.0002 | 0.0026 | 0 | 0.003 | 0.0014 | 0.0019 | 0.0003 | 0.0024 | 0 | 0.004 | 0 | 0.0003 | 0.0001 |
Vacuole Periphery | 0.002 | 0 | 0.0026 | 0 | 0.0027 | 0.0002 | 0.0016 | 0 | 0.0008 | 0.0005 | 0.0037 | 0.0002 | 0.0057 | 0 | 0.0029 | 0 | 0.0041 | 0.0001 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 18.8913 | 20.6787 | 17.9019 | 20.7129 | 23.4936 | 12.3303 | 16.2039 | 21.4027 | 16.3284 | 18.8163 |
Translational Efficiency | 0.6027 | 0.6125 | 0.6365 | 0.5799 | 0.5525 | 0.8385 | 0.854 | 0.5009 | 0.7705 | 0.6896 |
Dataset | Hit |
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Protein Concentration | – |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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